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Q08378 (GOGA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Golgin subfamily A member 3
Alternative name(s):
Golgi complex-associated protein of 170 kDa
Short name=GCP170
Golgin-160
Gene names
Name:GOLGA3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1498 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Golgi auto-antigen; probably involved in maintaining Golgi structure.

Subunit structure

Homodimer. Interacts with GOLGA7. Isoform 1 interacts with GOPC while isoform 3 does not. Ref.6 Ref.7 Ref.8

Subcellular location

Cytoplasm. Golgi apparatusGolgi stack membrane; Peripheral membrane protein Ref.1 Ref.2 Ref.6 Ref.7 Ref.8.

Tissue specificity

Expressed in all tissues tested. Expressed in liver, testis, lung, heart, salivary gland and kidney. Ref.8

Domain

Extended rod-like protein with coiled-coil domains.

Post-translational modification

Cleaved by caspases in apoptotic cells.

Sequence caution

The sequence AAA35921.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH60826.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence BAA23661.1 differs from that shown. Reason: Frameshift at position 1378.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q08378-1)

Also known as: Golgin-160B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q08378-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1382-1390: RKEPKGEAS → VLRPASLPG
     1391-1498: Missing.
Note: No experimental confirmation available. May be due to an intron retention.
Isoform 3 (identifier: Q08378-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1090-1134: LQESRGFRKK...GQSNAALREH → VRPGHLLWRQ...SVATFGVATF
     1135-1498: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14981498Golgin subfamily A member 3
PRO_0000190057

Regions

Region121 – 14121Interaction with GOPC
Region172 – 25786Golgi-targeting domain
Coiled coil394 – 14591066 Potential
Compositional bias20 – 9576Pro-rich
Compositional bias538 – 744207Gln-rich
Compositional bias1231 – 1369139Gln-rich

Sites

Site59 – 602Cleavage; by caspase-2
Site139 – 1402Cleavage; by caspase-3
Site311 – 3122Cleavage; by caspase-7

Amino acid modifications

Modified residue11N-acetylmethionine Ref.9 Ref.12 Ref.13
Modified residue571Phosphoserine By similarity
Modified residue3851Phosphoserine Ref.10
Modified residue9831Phosphoserine By similarity

Natural variations

Alternative sequence1090 – 113445LQESR…ALREH → VRPGHLLWRQRGAGHVSPGH AATRETRRTKLHRVPSVATF GVATF in isoform 3.
VSP_038000
Alternative sequence1135 – 1498364Missing in isoform 3.
VSP_038001
Alternative sequence1382 – 13909RKEPKGEAS → VLRPASLPG in isoform 2.
VSP_007728
Alternative sequence1391 – 1498108Missing in isoform 2.
VSP_007729
Natural variant701G → E.
Corresponds to variant rs2291256 [ dbSNP | Ensembl ].
VAR_020153
Natural variant2641P → L.
Corresponds to variant rs3741486 [ dbSNP | Ensembl ].
VAR_021901
Natural variant11851K → R.
Corresponds to variant rs2291260 [ dbSNP | Ensembl ].
VAR_020154

Experimental info

Mutagenesis591D → A: Abolishes cleavage by caspase-2. Ref.2
Mutagenesis1211L → A: Loss of interaction with GOPC; when associated with A-128 and A-135. Ref.8
Mutagenesis1281L → A: Loss of interaction with GOPC; when associated with A-121 and A-135. Ref.8
Mutagenesis1351L → A: Loss of interaction with GOPC; when associated with A-121 and A-128. Ref.8
Mutagenesis1391D → A: Abolishes cleavage by caspase-3. Ref.2
Mutagenesis3111D → A: Abolishes cleavage by caspase-7. Ref.2
Sequence conflict1591E → K in AAL93149. Ref.2
Sequence conflict6091L → I in BAA23661. Ref.1
Sequence conflict7461H → R in AAL93149. Ref.2
Sequence conflict7851A → V in AAA35921. Ref.4
Sequence conflict9321M → V in AAL93149. Ref.2
Sequence conflict10171K → R in AAL93149. Ref.2
Sequence conflict12811V → A in AAL93149. Ref.2
Sequence conflict13151R → W in AAL93149. Ref.2
Sequence conflict14431Q → R in AAL93149. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Golgin-160B) [UniParc].

Last modified July 3, 2003. Version 2.
Checksum: 4B95CDCFD3D64667

FASTA1,498167,355
        10         20         30         40         50         60 
MDGASAEQDG LQEDRSHSGP SSLPEAPLKP PGPLVPPDQQ DKVQCAEVNR ASTEGESPDG 

        70         80         90        100        110        120 
PGQGGLCQNG PTPPFPDPPS SLDPTTSPVG PDASPGVAGF HDNLRKSQGT SAEGSVRKEA 

       130        140        150        160        170        180 
LQSLRLSLPM QETQLCSTDS PLPLEKEEQV RLQARKWLEE QLKQYRVKRQ QERSSQPATK 

       190        200        210        220        230        240 
TRLFSTLDPE LMLNPENLPR ASTLAMTKEY SFLRTSVPRG PKVGSLGLPA HPREKKTSKS 

       250        260        270        280        290        300 
SKIRSLADYR TEDSNAGNSG GNVPAPDSTK GSLKQNRSSA ASVVSEISLS PDTDDRLENT 

       310        320        330        340        350        360 
SLAGDSVSEV DGNDSDSSSY SSASTRGTYG ILSKTVGTQD TPYMVNGQEI PADTLGQFPS 

       370        380        390        400        410        420 
IKDVLQAAAA EHQDQGQEVN GEVRSRRDSI CSSVSLESSA AETQEEMLQV LKEKMRLEGQ 

       430        440        450        460        470        480 
LEALSLEASQ ALKEKAELQA QLAALSTKLQ AQVECSHSSQ QRQDSLSSEV DTLKQSCWDL 

       490        500        510        520        530        540 
ERAMTDLQNM LEAKNASLAS SNNDLQVAEE QYQRLMAKVE DMQRSMLSKD NTVHDLRQQM 

       550        560        570        580        590        600 
TALQSQLQQV QLERTTLTSK LKASQAEISS LQSVRQWYQQ QLALAQEARV RLQGEMAHIQ 

       610        620        630        640        650        660 
VGQMTQAGLL EHLKLENVSL SQQLTETQHR SMKEKGRIAA QLQGIEADML DQEAAFMQIQ 

       670        680        690        700        710        720 
EAKTMVEEDL QRRLEEFEGE RERLQRMADS AASLEQQLEQ VKLTLLQRDQ QLEALQQEHL 

       730        740        750        760        770        780 
DLMKQLTLTQ EALQSREQSL DALQTHYDEL QARLGELQGE AASREDTICL LQNEKIILEA 

       790        800        810        820        830        840 
ALQAAKSGKE ELDRGARRLE EGTEETSETL EKLREELAIK SGQVEHLQQE TAALKKQMQK 

       850        860        870        880        890        900 
IKEQFLQQKV MVEAYRRDAT SKDQLISELK ATRKRLDSEL KELRQELMQV HGEKRTAEAE 

       910        920        930        940        950        960 
LSRLHREVAQ VRQHMADLEG HLQSAQKERD EMETHLQSLQ FDKEQMVAVT EANEALKKQI 

       970        980        990       1000       1010       1020 
EELQQEARKA ITEQKQKMRR LGSDLTSAQK EMKTKHKAYE NAVGILSRRL QEALAAKEAA 

      1030       1040       1050       1060       1070       1080 
DAELGQLRAQ GGSSDSSLAL HERIQALEAE LQAVSHSKTL LEKELQEVIA LTSQELEESR 

      1090       1100       1110       1120       1130       1140 
EKVLELEDEL QESRGFRKKI KRLEESNKKL ALELEHEKGK LTGLGQSNAA LREHNSILET 

      1150       1160       1170       1180       1190       1200 
ALAKREADLV QLNLQVQAVL QRKEEEDRQM KHLVQALQAS LEKEKEKVNS LKEQVAAAKV 

      1210       1220       1230       1240       1250       1260 
EAGHNRRHFK AASLELSEVK KELQAKEHLV QKLQAEADDL QIREGKHSQE IAQFQAELAE 

      1270       1280       1290       1300       1310       1320 
ARAQLQLLQK QLDEQLSKQP VGNQEMENLK WEVDQKEREI QSLKQQLDLT EQQGRKELEG 

      1330       1340       1350       1360       1370       1380 
LQQLLQNVKS ELEMAQEDLS MTQKDKFMLQ AKVSELKNNM KTLLQQNQQL KLDLRRGAAK 

      1390       1400       1410       1420       1430       1440 
TRKEPKGEAS SSNPATPIKI PDCPVPASLL EELLRPPPAV SKEPLKNLNS CLQQLKQEMD 

      1450       1460       1470       1480       1490 
SLQRQMEEHA LTVHESLSSW TPLEPATASP VPPGGHAGPR GDPQRHSQSR ASKEGPGE 

« Hide

Isoform 2 [UniParc].

Checksum: A7A2BA5DD87ACE30
Show »

FASTA1,390155,683
Isoform 3 [UniParc].

Checksum: 29EB52DCDD5B21D1
Show »

FASTA1,134126,129

References

« Hide 'large scale' references
[1]"Molecular characterization of GCP170, a 170-kDa protein associated with the cytoplasmic face of the Golgi membrane."
Misumi Y., Sohda M., Yano A., Fujiwara T., Ikehara Y.
J. Biol. Chem. 272:23851-23858(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PHOSPHORYLATION, SUBCELLULAR LOCATION.
Tissue: Pancreatic carcinoma.
[2]"Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis."
Mancini M., Machamer C.E., Roy S., Nicholson D.W., Thornberry N.A., Casciola-Rosen L.A., Rosen A.
J. Cell Biol. 149:603-612(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), MUTAGENESIS OF ASP-59; ASP-139 AND ASP-311, SUBCELLULAR LOCATION, PHOSPHORYLATION, CLEAVAGE BY CASPASES.
[3]"Molecular characterization of Mea-2/golgin-160/GCP170 gene encoding a Golgi membrane associated protein."
Kondo M., Matsukuma S., Hirose F., Matsuda M., Yoshihara M., Misumi Y., Aida M., Ikehara M., Sutou S.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-840 (ISOFORMS 1/2/3).
Tissue: Placenta.
[5]"Molecular characterization of two human autoantigens: unique cDNAs encoding 95- and 160-kD proteins of a putative family in the Golgi complex."
Fritzler M.J., Hamel J.C., Ochs R.L., Chan E.K.L.
J. Exp. Med. 178:49-62(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 780-1348.
Tissue: Liver.
[6]"The NH2-terminal domain of Golgin-160 contains both Golgi and nuclear targeting information."
Hicks S.W., Machamer C.E.
J. Biol. Chem. 277:35833-35839(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DIMERIZATION, SUBCELLULAR LOCATION.
[7]"Identification and characterization of GCP16, a novel acylated Golgi protein that interacts with GCP170."
Ohta E., Misumi Y., Sohda M., Fujiwara T., Yano A., Ikehara Y.
J. Biol. Chem. 278:51957-51967(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GOLGA7, SUBCELLULAR LOCATION.
[8]"Isoform-specific interaction of golgin-160 with the Golgi-associated protein PIST."
Hicks S.W., Machamer C.E.
J. Biol. Chem. 280:28944-28951(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GOPC, TISSUE SPECIFICITY, ALTERNATIVE SPLICING (ISOFORM 3), SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-121; LEU-128 AND LEU-135.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-385, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D63997 mRNA. Translation: BAA23661.1. Frameshift.
AF485338 mRNA. Translation: AAL93149.1.
AB027133 mRNA. Translation: BAB71953.1.
BC060826 mRNA. Translation: AAH60826.1. Sequence problems.
BC142658 mRNA. Translation: AAI42659.1.
BC146675 mRNA. Translation: AAI46676.1.
L06148 mRNA. Translation: AAA35921.1. Different initiation.
CCDSCCDS53846.1. [Q08378-4]
CCDS9281.1. [Q08378-1]
PIRJH0820.
RefSeqNP_001166028.1. NM_001172557.1. [Q08378-4]
NP_005886.2. NM_005895.3. [Q08378-1]
XP_005266219.1. XM_005266162.1. [Q08378-1]
XP_006719799.1. XM_006719736.1. [Q08378-1]
XP_006719800.1. XM_006719737.1. [Q08378-1]
XP_006719801.1. XM_006719738.1. [Q08378-1]
UniGeneHs.507333.

3D structure databases

ProteinModelPortalQ08378.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109064. 11 interactions.
IntActQ08378. 5 interactions.
MINTMINT-1157195.
STRING9606.ENSP00000204726.

PTM databases

PhosphoSiteQ08378.

Polymorphism databases

DMDM32470610.

Proteomic databases

MaxQBQ08378.
PaxDbQ08378.
PRIDEQ08378.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000204726; ENSP00000204726; ENSG00000090615. [Q08378-1]
ENST00000450791; ENSP00000410378; ENSG00000090615. [Q08378-1]
ENST00000456883; ENSP00000409303; ENSG00000090615. [Q08378-2]
ENST00000537452; ENSP00000442143; ENSG00000090615. [Q08378-4]
ENST00000545875; ENSP00000442603; ENSG00000090615. [Q08378-4]
GeneID2802.
KEGGhsa:2802.
UCSCuc001ukz.1. human. [Q08378-1]
uc001ula.1. human. [Q08378-2]
uc001ulb.3. human. [Q08378-4]

Organism-specific databases

CTD2802.
GeneCardsGC12M133345.
HGNCHGNC:4426. GOLGA3.
HPAHPA039809.
HPA040044.
MIM602581. gene.
neXtProtNX_Q08378.
PharmGKBPA28807.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG149564.
HOVERGENHBG051753.
InParanoidQ08378.
OMAQIREAKT.
OrthoDBEOG7XSTCV.
PhylomeDBQ08378.
TreeFamTF332014.

Gene expression databases

BgeeQ08378.
CleanExHS_GOLGA3.
GenevestigatorQ08378.

Family and domain databases

ProtoNetSearch...

Other

ChiTaRSGOLGA3. human.
GeneWikiGOLGA3.
GenomeRNAi2802.
NextBio11045.
PMAP-CutDBQ08378.
PROQ08378.
SOURCESearch...

Entry information

Entry nameGOGA3_HUMAN
AccessionPrimary (citable) accession number: Q08378
Secondary accession number(s): A5PKX6 expand/collapse secondary AC list , O43241, Q6P9C7, Q86XW3, Q8TDA9, Q8WZA3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 3, 2003
Last modified: July 9, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM