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Protein

Transcription factor GATA-4

Gene

Gata4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (By similarity). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (By similarity). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (PubMed:9584153). Promotes cardiac myocyte enlargement (By similarity). Required during testicular development (By similarity). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri216 – 240GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri270 – 294GATA-type 2PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • atrial septum morphogenesis Source: MGI
  • atrial septum primum morphogenesis Source: BHF-UCL
  • atrial septum secundum morphogenesis Source: MGI
  • atrioventricular canal development Source: BHF-UCL
  • atrioventricular valve formation Source: BHF-UCL
  • atrioventricular valve morphogenesis Source: MGI
  • BMP signaling pathway Source: MGI
  • canonical Wnt signaling pathway Source: MGI
  • cardiac muscle cell differentiation Source: MGI
  • cardiac muscle hypertrophy in response to stress Source: MGI
  • cardiac muscle tissue development Source: MGI
  • cardiac muscle tissue regeneration Source: BHF-UCL
  • cardiac right ventricle morphogenesis Source: BHF-UCL
  • cardiac septum development Source: BHF-UCL
  • cell-cell signaling Source: MGI
  • cellular response to follicle-stimulating hormone stimulus Source: MGI
  • cellular response to gonadotropin stimulus Source: MGI
  • diaphragm morphogenesis Source: BHF-UCL
  • embryonic digestive tract morphogenesis Source: MGI
  • embryonic foregut morphogenesis Source: MGI
  • embryonic heart tube anterior/posterior pattern specification Source: MGI
  • embryonic heart tube development Source: MGI
  • embryonic morphogenesis Source: MGI
  • endocardial cushion development Source: BHF-UCL
  • endoderm formation Source: MGI
  • epithelial cell fate commitment Source: MGI
  • gastrulation with mouth forming second Source: MGI
  • heart development Source: MGI
  • heart looping Source: BHF-UCL
  • heart morphogenesis Source: BHF-UCL
  • intestinal epithelial cell differentiation Source: MGI
  • in utero embryonic development Source: MGI
  • lung lobe formation Source: MGI
  • lung morphogenesis Source: BHF-UCL
  • male gonad development Source: MGI
  • mitral valve formation Source: BHF-UCL
  • negative regulation of apoptotic process Source: BHF-UCL
  • negative regulation of apoptotic signaling pathway Source: BHF-UCL
  • negative regulation of cardiac muscle cell apoptotic process Source: BHF-UCL
  • negative regulation of connective tissue replacement Source: BHF-UCL
  • negative regulation of gene expression Source: BHF-UCL
  • negative regulation of oxidative stress-induced cell death Source: BHF-UCL
  • outflow tract morphogenesis Source: BHF-UCL
  • positive regulation of angiogenesis Source: BHF-UCL
  • positive regulation of cardiac muscle cell proliferation Source: MGI
  • positive regulation of cardioblast differentiation Source: BHF-UCL
  • positive regulation of cell cycle Source: BHF-UCL
  • positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
  • positive regulation of gene expression Source: BHF-UCL
  • positive regulation of protein phosphorylation Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of vascular endothelial growth factor production Source: BHF-UCL
  • regulation of cardiac muscle cell proliferation Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of protein kinase B signaling Source: BHF-UCL
  • regulation of transcription, DNA-templated Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • response to drug Source: MGI
  • response to estrogen Source: MGI
  • response to retinoic acid Source: BHF-UCL
  • seminiferous tubule development Source: MGI
  • Sertoli cell differentiation Source: MGI
  • signal transduction involved in regulation of gene expression Source: MGI
  • skeletal muscle cell differentiation Source: UniProtKB
  • SMAD protein signal transduction Source: MGI
  • spermatogenesis Source: MGI
  • transcription from RNA polymerase II promoter Source: BHF-UCL
  • tricuspid valve formation Source: BHF-UCL
  • tube morphogenesis Source: MGI
  • vasculogenesis involved in coronary vascular morphogenesis Source: DFLAT
  • ventricular cardiac muscle tissue development Source: MGI
  • ventricular septum development Source: BHF-UCL

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor GATA-4
Alternative name(s):
GATA-binding factor 4
Gene namesi
Name:Gata4
Synonyms:Gata-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:95664. Gata4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1687680.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834141 – 441Transcription factor GATA-4Add BLAST441

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei299N6-methyllysine; by EZH21 Publication1

Post-translational modificationi

Methylation at Lys-299 attenuates transcriptional activity.1 Publication

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiQ08369.
PaxDbiQ08369.
PRIDEiQ08369.

PTM databases

iPTMnetiQ08369.
PhosphoSitePlusiQ08369.

Expressioni

Tissue specificityi

Heart, intestine, liver, primative endoderm and gonads.

Inductioni

By retinoic acid.

Gene expression databases

BgeeiENSMUSG00000021944.
CleanExiMM_GATA4.
ExpressionAtlasiQ08369. baseline and differential.
GenevisibleiQ08369. MM.

Interactioni

Subunit structurei

Interacts with ZNF260 (By similarity). Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with NFATC4 and LMCD1. Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes. Interacts with NR5A1, ZFPM2 and TBX5. Interacts with TBX18.By similarity5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199841. 18 interactors.
DIPiDIP-33032N.
IntActiQ08369. 4 interactors.
MINTiMINT-206005.
STRINGi10090.ENSMUSP00000113891.

Structurei

3D structure databases

ProteinModelPortaliQ08369.
SMRiQ08369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi118 – 126Poly-Ala9
Compositional biasi174 – 181Poly-Ala8
Compositional biasi275 – 279Poly-Thr5
Compositional biasi354 – 359Poly-Ser6

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri216 – 240GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri270 – 294GATA-type 2PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047700.
HOVERGENiHBG051703.
InParanoidiQ08369.
KOiK09183.
PhylomeDBiQ08369.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiView protein in InterPro
IPR008013. GATA_N.
IPR016375. TF_GATA_4/5/6.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
PfamiView protein in Pfam
PF00320. GATA. 2 hits.
PF05349. GATA-N. 1 hit.
PIRSFiPIRSF003028. TF_GATA_4/5/6. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiView protein in SMART
SM00401. ZnF_GATA. 2 hits.
PROSITEiView protein in PROSITE
PS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.

Sequencei

Sequence statusi: Complete.

Q08369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYQSLAMAAN HGPPPGAYEA GGPGAFMHSA GAASSPVYVP TPRVPSSVLG
60 70 80 90 100
LSYLQGGGSA AAAGTTSGGS SGAGPSGAGP GTQQGSPGWS QAGAEGAAYT
110 120 130 140 150
PPPVSPRFSF PGTTGSLAAA AAAAAAREAA AYGSGGGAAG AGLAGREQYG
160 170 180 190 200
RPGFAGSYSS PYPAYMADVG ASWAAAAAAS AGPFDSPVLH SLPGRANPGR
210 220 230 240 250
HPNLDMFDDF SEGRECVNCG AMSTPLWRRD GTGHYLCNAC GLYHKMNGIN
260 270 280 290 300
RPLIKPQRRL SASRRVGLSC ANCQTTTTTL WRRNAEGEPV CNACGLYMKL
310 320 330 340 350
HGVPRPLAMR KEGIQTRKRK PKNLNKSKTP AGPAGETLPP SSGASSGNSS
360 370 380 390 400
NATSSSSSSE EMRPIKTEPG LSSHYGHSSS MSQTFSTVSG HGPSIHPVLS
410 420 430 440
ALKLSPQGYA SPVTQTSQAS SKQDSWNSLV LADSHGDIIT A
Length:441
Mass (Da):44,580
Last modified:March 19, 2014 - v4
Checksum:iE9521771741DD08E
GO

Sequence cautioni

The sequence AAA37662 differs from that shown. Reason: Frameshift at positions 6, 40 and 69.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti385 – 402FSTVS…VLSAL → SVCVRPRALHPSSAVCS in AAA37662 (PubMed:8455608).CuratedAdd BLAST18
Sequence conflicti385 – 402FSTVS…VLSAL → SVCVRPRALHPSSAVCS in AAB42015 (Ref. 3) CuratedAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98339 mRNA. Translation: AAA37662.1. Frameshift.
U85046 mRNA. Translation: AAB42015.1.
AF179424 mRNA. Translation: AAD55266.1.
AC090654 Genomic DNA. No translation available.
AC090962 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36067.1.
BC137824 mRNA. Translation: AAI37825.1.
CCDSiCCDS49519.1.
PIRiA48099.
RefSeqiNP_032118.2. NM_008092.4.
UniGeneiMm.247669.

Genome annotation databases

EnsembliENSMUST00000118022; ENSMUSP00000113891; ENSMUSG00000021944.
GeneIDi14463.
KEGGimmu:14463.
UCSCiuc007uhn.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiGATA4_MOUSE
AccessioniPrimary (citable) accession number: Q08369
Secondary accession number(s): B9EHF7, P97491, Q9QZK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: March 19, 2014
Last modified: September 27, 2017
This is version 159 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot