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Q08369 (GATA4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor GATA-4
Alternative name(s):
GATA-binding factor 4
Gene names
Name:Gata4
Synonyms:Gata-4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length441 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. Ref.10

Subunit structure

Interacts with ZNF260 By similarity. Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with NFATC4 and LMCD1. Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes. Interacts with NR5A1 and ZFPM2 By similarity. Ref.9 Ref.10 Ref.11 Ref.12

Subcellular location

Nucleus.

Tissue specificity

Heart, intestine, liver, primative endoderm and gonads.

Induction

By retinoic acid.

Post-translational modification

Methylation at Lys-299 attenuates transcriptional activity. Ref.13

Sequence similarities

Contains 2 GATA-type zinc fingers.

Sequence caution

The sequence AAA37662.1 differs from that shown. Reason: Frameshift at positions 6, 40 and 69.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   PTMMethylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processBMP signaling pathway

Inferred from direct assay PubMed 17360443. Source: MGI

SMAD protein signal transduction

Inferred from direct assay PubMed 9420335. Source: MGI

Sertoli cell differentiation

Inferred from mutant phenotype PubMed 21172404. Source: MGI

atrial septum primum morphogenesis

Inferred from mutant phenotype PubMed 17643447. Source: BHF-UCL

atrial septum secundum morphogenesis

Inferred from electronic annotation. Source: Ensembl

atrioventricular canal development

Inferred from genetic interaction PubMed 21330551. Source: BHF-UCL

atrioventricular valve formation

Inferred from genetic interaction PubMed 21330551. Source: BHF-UCL

atrioventricular valve morphogenesis

Inferred from genetic interaction. Source: MGI

canonical Wnt signaling pathway

Inferred from direct assay PubMed 17360443. Source: MGI

cardiac muscle cell differentiation

Inferred from mutant phenotype. Source: MGI

cardiac muscle hypertrophy in response to stress

Inferred from mutant phenotype PubMed 20705924. Source: MGI

cardiac muscle tissue development

Inferred from genetic interaction PubMed 20705924. Source: MGI

cardiac right ventricle morphogenesis

Inferred from mutant phenotype PubMed 17643447. Source: BHF-UCL

cardiac septum development

Inferred from genetic interaction PubMed 21330551. Source: BHF-UCL

cell growth involved in cardiac muscle cell development

Inferred from electronic annotation. Source: Ensembl

cell-cell signaling

Inferred from mutant phenotype PubMed 16127717. Source: MGI

cellular response to follicle-stimulating hormone stimulus

Inferred from direct assay PubMed 9231805. Source: MGI

cellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to gonadotropin stimulus

Inferred from direct assay PubMed 9231805. Source: MGI

diaphragm morphogenesis

Traceable author statement PubMed 17643447. Source: BHF-UCL

embryonic digestive tract morphogenesis

Inferred from mutant phenotype PubMed 16127717. Source: MGI

embryonic foregut morphogenesis

Inferred from mutant phenotype PubMed 9136932PubMed 9136933. Source: MGI

embryonic heart tube anterior/posterior pattern specification

Inferred from mutant phenotype PubMed 9136932. Source: MGI

embryonic heart tube development

Inferred from mutant phenotype PubMed 9136932PubMed 9136933. Source: MGI

embryonic morphogenesis

Inferred from mutant phenotype PubMed 9136932PubMed 9136933. Source: MGI

endocardial cushion development

Inferred from genetic interaction. Source: MGI

endoderm formation

Inferred from mutant phenotype PubMed 8582296. Source: MGI

epithelial cell fate commitment

Inferred from mutant phenotype PubMed 16940177. Source: MGI

gastrulation with mouth forming second

Inferred from mutant phenotype PubMed 9136933. Source: MGI

heart development

Inferred from mutant phenotype PubMed 11297508PubMed 15464586. Source: MGI

heart looping

Inferred from mutant phenotype PubMed 15310850. Source: BHF-UCL

heart morphogenesis

Inferred from mutant phenotype PubMed 17975667. Source: BHF-UCL

in utero embryonic development

Inferred from mutant phenotype PubMed 15310850PubMed 20123909PubMed 9136932PubMed 9136933. Source: MGI

intestinal epithelial cell differentiation

Inferred from sequence orthology PubMed 9566909. Source: MGI

lung lobe formation

Inferred from mutant phenotype PubMed 17142311. Source: MGI

lung morphogenesis

Traceable author statement PubMed 17643447. Source: BHF-UCL

mitral valve formation

Traceable author statement PubMed 14614148. Source: BHF-UCL

negative regulation of autophagy

Inferred from electronic annotation. Source: Ensembl

outflow tract morphogenesis

Traceable author statement PubMed 14614148. Source: BHF-UCL

positive regulation of BMP signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of angiogenesis

Inferred from direct assay PubMed 17975667. Source: BHF-UCL

positive regulation of cardiac muscle cell proliferation

Inferred from genetic interaction PubMed 19084512. Source: MGI

positive regulation of cardioblast differentiation

Inferred from expression pattern PubMed 16137232. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from direct assay. Source: MGI

positive regulation vascular endothelial growth factor production

Inferred from direct assay PubMed 17975667. Source: BHF-UCL

regulation of cardiac muscle cell contraction

Inferred from electronic annotation. Source: Ensembl

regulation of cardiac muscle cell proliferation

Inferred from mutant phenotype PubMed 15902305. Source: MGI

regulation of gene expression

Inferred from direct assay PubMed 20833366. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 12606418PubMed 14978031PubMed 15082719PubMed 8007990Ref.1. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to estrogen

Inferred from direct assay PubMed 9231805. Source: MGI

response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

response to retinoic acid

Inferred from direct assay PubMed 18439490. Source: BHF-UCL

seminiferous tubule development

Inferred from mutant phenotype PubMed 21172404. Source: MGI

signal transduction involved in regulation of gene expression

Inferred from direct assay PubMed 18462699. Source: MGI

skeletal muscle cell differentiation

Non-traceable author statement PubMed 21556048. Source: UniProtKB

spermatogenesis

Inferred from mutant phenotype PubMed 21172404. Source: MGI

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 12845333. Source: BHF-UCL

tricuspid valve formation

Traceable author statement PubMed 14614148. Source: BHF-UCL

tube morphogenesis

Inferred from genetic interaction PubMed 18400219. Source: MGI

vasculogenesis involved in coronary vascular morphogenesis

Traceable author statement PubMed 20299672. Source: DFLAT

ventricular cardiac muscle tissue development

Inferred from genetic interaction PubMed 19084512. Source: MGI

ventricular septum development

Inferred from mutant phenotype PubMed 17643447. Source: BHF-UCL

   Cellular_componentnuclear chromatin

Inferred from direct assay PubMed 21029371. Source: BHF-UCL

nucleus

Inferred from direct assay. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 15539431PubMed 8007990PubMed 9738004. Source: MGI

RNA polymerase II core promoter proximal region sequence-specific DNA binding

Inferred from direct assay PubMed 21029371. Source: BHF-UCL

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay. Source: MGI

RNA polymerase II regulatory region sequence-specific DNA binding

Inferred from direct assay PubMed 16603706. Source: MGI

RNA polymerase II transcription factor binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

chromatin binding

Inferred from direct assay PubMed 21379568. Source: MGI

enhancer sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

sequence-specific DNA binding

Inferred from direct assay Ref.1PubMed 8754853. Source: MGI

sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from direct assay. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 15082719PubMed 9738004. Source: MGI

transcription regulatory region DNA binding

Inferred from sequence orthology PubMed 9566909. Source: MGI

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Jarid2Q623153EBI-297008,EBI-493592
Nkx2-5P425824EBI-297008,EBI-297021
TBX5Q995932EBI-297008,EBI-297043From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 441441Transcription factor GATA-4
PRO_0000083414

Regions

Zinc finger216 – 24025GATA-type 1
Zinc finger270 – 29425GATA-type 2
Compositional bias118 – 1269Poly-Ala
Compositional bias174 – 1818Poly-Ala
Compositional bias275 – 2795Poly-Thr
Compositional bias354 – 3596Poly-Ser

Amino acid modifications

Modified residue2991N6-methyllysine; by EZH2 Ref.13

Experimental info

Sequence conflict385 – 40218FSTVS…VLSAL → SVCVRPRALHPSSAVCS in AAA37662. Ref.1
Sequence conflict385 – 40218FSTVS…VLSAL → SVCVRPRALHPSSAVCS in AAB42015. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q08369 [UniParc].

Last modified March 19, 2014. Version 4.
Checksum: E9521771741DD08E

FASTA44144,580
        10         20         30         40         50         60 
MYQSLAMAAN HGPPPGAYEA GGPGAFMHSA GAASSPVYVP TPRVPSSVLG LSYLQGGGSA 

        70         80         90        100        110        120 
AAAGTTSGGS SGAGPSGAGP GTQQGSPGWS QAGAEGAAYT PPPVSPRFSF PGTTGSLAAA 

       130        140        150        160        170        180 
AAAAAAREAA AYGSGGGAAG AGLAGREQYG RPGFAGSYSS PYPAYMADVG ASWAAAAAAS 

       190        200        210        220        230        240 
AGPFDSPVLH SLPGRANPGR HPNLDMFDDF SEGRECVNCG AMSTPLWRRD GTGHYLCNAC 

       250        260        270        280        290        300 
GLYHKMNGIN RPLIKPQRRL SASRRVGLSC ANCQTTTTTL WRRNAEGEPV CNACGLYMKL 

       310        320        330        340        350        360 
HGVPRPLAMR KEGIQTRKRK PKNLNKSKTP AGPAGETLPP SSGASSGNSS NATSSSSSSE 

       370        380        390        400        410        420 
EMRPIKTEPG LSSHYGHSSS MSQTFSTVSG HGPSIHPVLS ALKLSPQGYA SPVTQTSQAS 

       430        440 
SKQDSWNSLV LADSHGDIIT A 

« Hide

References

« Hide 'large scale' references
[1]"Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart."
Arceci R.J., King A.A., Simon M.C., Orkin S.H., Wilson D.B.
Mol. Cell. Biol. 13:2235-2246(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"GATA-4/5/6, a subfamily of three transcription factors transcribed in developing heart and gut."
Laverriere A.C., Macneill C., Mueller C., Poelmann R.E., Burch J.B.E., Evans T.
J. Biol. Chem. 269:23177-23184(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFTS.
[3]"Murine GATA-4 is expressed in heart and gut tissues."
Morrisey E.E., Parmacek M.S.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]Katsuoka F., Motohashi H., Yamamoto M.
Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X DBA/2.
Tissue: Heart.
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[6]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[8]"Regulation of J6 gene expression by transcription factor GATA-4."
Bielinska M., Wilson D.B.
Biochem. J. 307:183-189(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[9]"A calcineurin-dependent transcriptional pathway for cardiac hypertrophy."
Molkentin J.D., Lu J.-R., Antos C.L., Markham B., Richardson J., Robbins J., Grant S.R., Olson E.N.
Cell 93:215-228(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NFATC4.
[10]"The cardiac tissue-restricted homeobox protein Csx/Nkx2.5 physically associates with the zinc finger protein GATA4 and cooperatively activates atrial natriuretic factor gene expression."
Lee Y., Shioi T., Kasahara H., Jobe S.M., Wiese R.J., Markham B.E., Izumo S.
Mol. Cell. Biol. 18:3120-3129(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NKX2-5.
[11]"Jumonji represses atrial natriuretic factor gene expression by inhibiting transcriptional activities of cardiac transcription factors."
Kim T.-G., Chen J., Sadoshima J., Lee Y.
Mol. Cell. Biol. 24:10151-10160(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH JARID2.
[12]"LMCD1/Dyxin is a novel transcriptional cofactor that restricts GATA6 function by inhibiting DNA binding."
Rath N., Wang Z., Lu M.M., Morrisey E.E.
Mol. Cell. Biol. 25:8864-8873(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LMCD1.
[13]"PRC2 directly methylates GATA4 and represses its transcriptional activity."
He A., Shen X., Ma Q., Cao J., von Gise A., Zhou P., Wang G., Marquez V.E., Orkin S.H., Pu W.T.
Genes Dev. 26:37-42(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT LYS-299.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M98339 mRNA. Translation: AAA37662.1. Frameshift.
U85046 mRNA. Translation: AAB42015.1.
AF179424 mRNA. Translation: AAD55266.1.
AC090654 Genomic DNA. No translation available.
AC090962 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36067.1.
BC137824 mRNA. Translation: AAI37825.1.
PIRA48099.
RefSeqNP_032118.2. NM_008092.3.
UniGeneMm.247669.

3D structure databases

ProteinModelPortalQ08369.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199841. 17 interactions.
DIPDIP-33032N.
IntActQ08369. 3 interactions.
MINTMINT-206005.

Chemistry

ChEMBLCHEMBL1687680.

PTM databases

PhosphoSiteQ08369.

Proteomic databases

PaxDbQ08369.
PRIDEQ08369.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000118022; ENSMUSP00000113891; ENSMUSG00000021944.
GeneID14463.
KEGGmmu:14463.

Organism-specific databases

CTD2626.
MGIMGI:95664. Gata4.

Phylogenomic databases

eggNOGCOG5641.
GeneTreeENSGT00750000117615.
HOVERGENHBG051703.
KOK09183.

Gene expression databases

CleanExMM_GATA4.
GenevestigatorQ08369.

Family and domain databases

Gene3D3.30.50.10. 2 hits.
InterProIPR008013. GATA_N.
IPR028436. TF_GATA_4.
IPR016375. TF_GATA_4/5/6.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERPTHR10071:SF25. PTHR10071:SF25. 1 hit.
PfamPF00320. GATA. 2 hits.
PF05349. GATA-N. 1 hit.
[Graphical view]
PIRSFPIRSF003028. TF_GATA_4/5/6. 1 hit.
PRINTSPR00619. GATAZNFINGER.
SMARTSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio286104.
PROQ08369.
SOURCESearch...

Entry information

Entry nameGATA4_MOUSE
AccessionPrimary (citable) accession number: Q08369
Secondary accession number(s): B9EHF7, P97491, Q9QZK4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: March 19, 2014
Last modified: April 16, 2014
This is version 126 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot