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Q08369

- GATA4_MOUSE

UniProt

Q08369 - GATA4_MOUSE

Protein

Transcription factor GATA-4

Gene

Gata4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 4 (19 Mar 2014)
      Previous versions | rss
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    Functioni

    Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri216 – 24025GATA-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri270 – 29425GATA-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: MGI
    3. protein binding Source: UniProtKB
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: BHF-UCL
    5. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: BHF-UCL
    6. RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
    7. RNA polymerase II transcription factor binding transcription factor activity Source: UniProtKB
    8. sequence-specific DNA binding Source: MGI
    9. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: BHF-UCL
    10. sequence-specific DNA binding transcription factor activity Source: MGI
    11. transcription regulatory region DNA binding Source: MGI
    12. zinc ion binding Source: InterPro

    GO - Biological processi

    1. atrial septum primum morphogenesis Source: BHF-UCL
    2. atrioventricular canal development Source: BHF-UCL
    3. atrioventricular valve formation Source: BHF-UCL
    4. atrioventricular valve morphogenesis Source: BHF-UCL
    5. BMP signaling pathway Source: MGI
    6. canonical Wnt signaling pathway Source: MGI
    7. cardiac muscle cell differentiation Source: UniProtKB
    8. cardiac muscle hypertrophy in response to stress Source: MGI
    9. cardiac muscle tissue development Source: MGI
    10. cardiac right ventricle morphogenesis Source: BHF-UCL
    11. cardiac septum development Source: BHF-UCL
    12. cell-cell signaling Source: MGI
    13. cellular response to follicle-stimulating hormone stimulus Source: MGI
    14. cellular response to gonadotropin stimulus Source: MGI
    15. diaphragm morphogenesis Source: BHF-UCL
    16. embryonic digestive tract morphogenesis Source: MGI
    17. embryonic foregut morphogenesis Source: MGI
    18. embryonic heart tube anterior/posterior pattern specification Source: MGI
    19. embryonic heart tube development Source: MGI
    20. embryonic morphogenesis Source: MGI
    21. endocardial cushion development Source: BHF-UCL
    22. endoderm formation Source: MGI
    23. epithelial cell fate commitment Source: MGI
    24. gastrulation with mouth forming second Source: MGI
    25. heart development Source: MGI
    26. heart looping Source: BHF-UCL
    27. heart morphogenesis Source: BHF-UCL
    28. intestinal epithelial cell differentiation Source: MGI
    29. in utero embryonic development Source: MGI
    30. lung lobe formation Source: MGI
    31. lung morphogenesis Source: BHF-UCL
    32. mitral valve formation Source: BHF-UCL
    33. outflow tract morphogenesis Source: BHF-UCL
    34. positive regulation of angiogenesis Source: BHF-UCL
    35. positive regulation of cardiac muscle cell proliferation Source: MGI
    36. positive regulation of cardioblast differentiation Source: BHF-UCL
    37. positive regulation of transcription, DNA-templated Source: UniProtKB
    38. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    39. positive regulation vascular endothelial growth factor production Source: BHF-UCL
    40. regulation of cardiac muscle cell proliferation Source: MGI
    41. regulation of gene expression Source: MGI
    42. regulation of transcription, DNA-templated Source: DFLAT
    43. regulation of transcription from RNA polymerase II promoter Source: MGI
    44. response to estrogen Source: MGI
    45. response to retinoic acid Source: BHF-UCL
    46. seminiferous tubule development Source: MGI
    47. Sertoli cell differentiation Source: MGI
    48. signal transduction involved in regulation of gene expression Source: MGI
    49. skeletal muscle cell differentiation Source: UniProtKB
    50. SMAD protein signal transduction Source: MGI
    51. spermatogenesis Source: MGI
    52. transcription from RNA polymerase II promoter Source: BHF-UCL
    53. tricuspid valve formation Source: BHF-UCL
    54. tube morphogenesis Source: MGI
    55. vasculogenesis involved in coronary vascular morphogenesis Source: DFLAT
    56. ventricular cardiac muscle tissue development Source: MGI
    57. ventricular septum development Source: BHF-UCL

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor GATA-4
    Alternative name(s):
    GATA-binding factor 4
    Gene namesi
    Name:Gata4
    Synonyms:Gata-4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:95664. Gata4.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear chromatin Source: BHF-UCL
    2. nucleus Source: BHF-UCL

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 441441Transcription factor GATA-4PRO_0000083414Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei299 – 2991N6-methyllysine; by EZH21 Publication

    Post-translational modificationi

    Methylation at Lys-299 attenuates transcriptional activity.1 Publication

    Keywords - PTMi

    Methylation

    Proteomic databases

    PaxDbiQ08369.
    PRIDEiQ08369.

    PTM databases

    PhosphoSiteiQ08369.

    Expressioni

    Tissue specificityi

    Heart, intestine, liver, primative endoderm and gonads.

    Inductioni

    By retinoic acid.

    Gene expression databases

    CleanExiMM_GATA4.
    GenevestigatoriQ08369.

    Interactioni

    Subunit structurei

    Interacts with ZNF260 By similarity. Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with NFATC4 and LMCD1. Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes. Interacts with NR5A1 and ZFPM2 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Jarid2Q623153EBI-297008,EBI-493592
    Nkx2-5P425824EBI-297008,EBI-297021
    TBX5Q995932EBI-297008,EBI-297043From a different organism.

    Protein-protein interaction databases

    BioGridi199841. 18 interactions.
    DIPiDIP-33032N.
    IntActiQ08369. 3 interactions.
    MINTiMINT-206005.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08369.
    SMRiQ08369. Positions 212-320.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi118 – 1269Poly-Ala
    Compositional biasi174 – 1818Poly-Ala
    Compositional biasi275 – 2795Poly-Thr
    Compositional biasi354 – 3596Poly-Ser

    Sequence similaritiesi

    Contains 2 GATA-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri216 – 24025GATA-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri270 – 29425GATA-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5641.
    GeneTreeiENSGT00750000117615.
    HOVERGENiHBG051703.
    KOiK09183.
    PhylomeDBiQ08369.

    Family and domain databases

    Gene3Di3.30.50.10. 2 hits.
    InterProiIPR008013. GATA_N.
    IPR028436. TF_GATA_4.
    IPR016375. TF_GATA_4/5/6.
    IPR000679. Znf_GATA.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PANTHERiPTHR10071:SF154. PTHR10071:SF154. 1 hit.
    PfamiPF00320. GATA. 2 hits.
    PF05349. GATA-N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003028. TF_GATA_4/5/6. 1 hit.
    PRINTSiPR00619. GATAZNFINGER.
    SMARTiSM00401. ZnF_GATA. 2 hits.
    [Graphical view]
    PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
    PS50114. GATA_ZN_FINGER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08369-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYQSLAMAAN HGPPPGAYEA GGPGAFMHSA GAASSPVYVP TPRVPSSVLG    50
    LSYLQGGGSA AAAGTTSGGS SGAGPSGAGP GTQQGSPGWS QAGAEGAAYT 100
    PPPVSPRFSF PGTTGSLAAA AAAAAAREAA AYGSGGGAAG AGLAGREQYG 150
    RPGFAGSYSS PYPAYMADVG ASWAAAAAAS AGPFDSPVLH SLPGRANPGR 200
    HPNLDMFDDF SEGRECVNCG AMSTPLWRRD GTGHYLCNAC GLYHKMNGIN 250
    RPLIKPQRRL SASRRVGLSC ANCQTTTTTL WRRNAEGEPV CNACGLYMKL 300
    HGVPRPLAMR KEGIQTRKRK PKNLNKSKTP AGPAGETLPP SSGASSGNSS 350
    NATSSSSSSE EMRPIKTEPG LSSHYGHSSS MSQTFSTVSG HGPSIHPVLS 400
    ALKLSPQGYA SPVTQTSQAS SKQDSWNSLV LADSHGDIIT A 441
    Length:441
    Mass (Da):44,580
    Last modified:March 19, 2014 - v4
    Checksum:iE9521771741DD08E
    GO

    Sequence cautioni

    The sequence AAA37662.1 differs from that shown. Reason: Frameshift at positions 6, 40 and 69.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti385 – 40218FSTVS…VLSAL → SVCVRPRALHPSSAVCS in AAA37662. (PubMed:8455608)CuratedAdd
    BLAST
    Sequence conflicti385 – 40218FSTVS…VLSAL → SVCVRPRALHPSSAVCS in AAB42015. 1 PublicationCuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98339 mRNA. Translation: AAA37662.1. Frameshift.
    U85046 mRNA. Translation: AAB42015.1.
    AF179424 mRNA. Translation: AAD55266.1.
    AC090654 Genomic DNA. No translation available.
    AC090962 Genomic DNA. No translation available.
    CH466535 Genomic DNA. Translation: EDL36067.1.
    BC137824 mRNA. Translation: AAI37825.1.
    CCDSiCCDS49519.1.
    PIRiA48099.
    RefSeqiNP_032118.2. NM_008092.3.
    UniGeneiMm.247669.

    Genome annotation databases

    EnsembliENSMUST00000118022; ENSMUSP00000113891; ENSMUSG00000021944.
    GeneIDi14463.
    KEGGimmu:14463.
    UCSCiuc007uhn.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98339 mRNA. Translation: AAA37662.1 . Frameshift.
    U85046 mRNA. Translation: AAB42015.1 .
    AF179424 mRNA. Translation: AAD55266.1 .
    AC090654 Genomic DNA. No translation available.
    AC090962 Genomic DNA. No translation available.
    CH466535 Genomic DNA. Translation: EDL36067.1 .
    BC137824 mRNA. Translation: AAI37825.1 .
    CCDSi CCDS49519.1.
    PIRi A48099.
    RefSeqi NP_032118.2. NM_008092.3.
    UniGenei Mm.247669.

    3D structure databases

    ProteinModelPortali Q08369.
    SMRi Q08369. Positions 212-320.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199841. 18 interactions.
    DIPi DIP-33032N.
    IntActi Q08369. 3 interactions.
    MINTi MINT-206005.

    Chemistry

    ChEMBLi CHEMBL1687680.

    PTM databases

    PhosphoSitei Q08369.

    Proteomic databases

    PaxDbi Q08369.
    PRIDEi Q08369.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000118022 ; ENSMUSP00000113891 ; ENSMUSG00000021944 .
    GeneIDi 14463.
    KEGGi mmu:14463.
    UCSCi uc007uhn.1. mouse.

    Organism-specific databases

    CTDi 2626.
    MGIi MGI:95664. Gata4.

    Phylogenomic databases

    eggNOGi COG5641.
    GeneTreei ENSGT00750000117615.
    HOVERGENi HBG051703.
    KOi K09183.
    PhylomeDBi Q08369.

    Enzyme and pathway databases

    Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

    Miscellaneous databases

    NextBioi 286104.
    PROi Q08369.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_GATA4.
    Genevestigatori Q08369.

    Family and domain databases

    Gene3Di 3.30.50.10. 2 hits.
    InterProi IPR008013. GATA_N.
    IPR028436. TF_GATA_4.
    IPR016375. TF_GATA_4/5/6.
    IPR000679. Znf_GATA.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    PANTHERi PTHR10071:SF154. PTHR10071:SF154. 1 hit.
    Pfami PF00320. GATA. 2 hits.
    PF05349. GATA-N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003028. TF_GATA_4/5/6. 1 hit.
    PRINTSi PR00619. GATAZNFINGER.
    SMARTi SM00401. ZnF_GATA. 2 hits.
    [Graphical view ]
    PROSITEi PS00344. GATA_ZN_FINGER_1. 2 hits.
    PS50114. GATA_ZN_FINGER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart."
      Arceci R.J., King A.A., Simon M.C., Orkin S.H., Wilson D.B.
      Mol. Cell. Biol. 13:2235-2246(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "GATA-4/5/6, a subfamily of three transcription factors transcribed in developing heart and gut."
      Laverriere A.C., Macneill C., Mueller C., Poelmann R.E., Burch J.B.E., Evans T.
      J. Biol. Chem. 269:23177-23184(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFTS.
    3. "Murine GATA-4 is expressed in heart and gut tissues."
      Morrisey E.E., Parmacek M.S.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. Katsuoka F., Motohashi H., Yamamoto M.
      Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X DBA/2.
      Tissue: Heart.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    6. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    8. "Regulation of J6 gene expression by transcription factor GATA-4."
      Bielinska M., Wilson D.B.
      Biochem. J. 307:183-189(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    9. "A calcineurin-dependent transcriptional pathway for cardiac hypertrophy."
      Molkentin J.D., Lu J.-R., Antos C.L., Markham B., Richardson J., Robbins J., Grant S.R., Olson E.N.
      Cell 93:215-228(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NFATC4.
    10. "The cardiac tissue-restricted homeobox protein Csx/Nkx2.5 physically associates with the zinc finger protein GATA4 and cooperatively activates atrial natriuretic factor gene expression."
      Lee Y., Shioi T., Kasahara H., Jobe S.M., Wiese R.J., Markham B.E., Izumo S.
      Mol. Cell. Biol. 18:3120-3129(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NKX2-5.
    11. "Jumonji represses atrial natriuretic factor gene expression by inhibiting transcriptional activities of cardiac transcription factors."
      Kim T.-G., Chen J., Sadoshima J., Lee Y.
      Mol. Cell. Biol. 24:10151-10160(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH JARID2.
    12. "LMCD1/Dyxin is a novel transcriptional cofactor that restricts GATA6 function by inhibiting DNA binding."
      Rath N., Wang Z., Lu M.M., Morrisey E.E.
      Mol. Cell. Biol. 25:8864-8873(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LMCD1.
    13. "PRC2 directly methylates GATA4 and represses its transcriptional activity."
      He A., Shen X., Ma Q., Cao J., von Gise A., Zhou P., Wang G., Marquez V.E., Orkin S.H., Pu W.T.
      Genes Dev. 26:37-42(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT LYS-299.

    Entry informationi

    Entry nameiGATA4_MOUSE
    AccessioniPrimary (citable) accession number: Q08369
    Secondary accession number(s): B9EHF7, P97491, Q9QZK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: March 19, 2014
    Last modified: October 1, 2014
    This is version 131 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3