Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor GATA-4

Gene

Gata4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri216 – 24025GATA-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri270 – 29425GATA-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • atrial septum morphogenesis Source: MGI
  • atrial septum primum morphogenesis Source: BHF-UCL
  • atrial septum secundum morphogenesis Source: MGI
  • atrioventricular canal development Source: BHF-UCL
  • atrioventricular valve formation Source: BHF-UCL
  • atrioventricular valve morphogenesis Source: BHF-UCL
  • BMP signaling pathway Source: MGI
  • canonical Wnt signaling pathway Source: MGI
  • cardiac muscle cell differentiation Source: UniProtKB
  • cardiac muscle hypertrophy in response to stress Source: MGI
  • cardiac muscle tissue development Source: MGI
  • cardiac right ventricle morphogenesis Source: BHF-UCL
  • cardiac septum development Source: BHF-UCL
  • cell-cell signaling Source: MGI
  • cell growth involved in cardiac muscle cell development Source: Ensembl
  • cellular response to follicle-stimulating hormone stimulus Source: MGI
  • cellular response to glucose stimulus Source: Ensembl
  • cellular response to gonadotropin stimulus Source: MGI
  • diaphragm morphogenesis Source: BHF-UCL
  • embryonic digestive tract morphogenesis Source: MGI
  • embryonic foregut morphogenesis Source: MGI
  • embryonic heart tube anterior/posterior pattern specification Source: MGI
  • embryonic heart tube development Source: MGI
  • embryonic morphogenesis Source: MGI
  • endocardial cushion development Source: BHF-UCL
  • endoderm formation Source: MGI
  • epithelial cell fate commitment Source: MGI
  • gastrulation with mouth forming second Source: MGI
  • heart development Source: MGI
  • heart looping Source: BHF-UCL
  • heart morphogenesis Source: BHF-UCL
  • intestinal epithelial cell differentiation Source: MGI
  • in utero embryonic development Source: MGI
  • lung lobe formation Source: MGI
  • lung morphogenesis Source: BHF-UCL
  • male gonad development Source: MGI
  • mitral valve formation Source: BHF-UCL
  • negative regulation of autophagy Source: Ensembl
  • outflow tract morphogenesis Source: BHF-UCL
  • positive regulation of angiogenesis Source: BHF-UCL
  • positive regulation of BMP signaling pathway Source: Ensembl
  • positive regulation of cardiac muscle cell proliferation Source: MGI
  • positive regulation of cardioblast differentiation Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of vascular endothelial growth factor production Source: BHF-UCL
  • regulation of cardiac muscle cell contraction Source: Ensembl
  • regulation of cardiac muscle cell proliferation Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of transcription, DNA-templated Source: DFLAT
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • response to drug Source: MGI
  • response to estrogen Source: MGI
  • response to mechanical stimulus Source: Ensembl
  • response to retinoic acid Source: BHF-UCL
  • response to vitamin A Source: Ensembl
  • seminiferous tubule development Source: MGI
  • Sertoli cell differentiation Source: MGI
  • signal transduction involved in regulation of gene expression Source: MGI
  • skeletal muscle cell differentiation Source: UniProtKB
  • SMAD protein signal transduction Source: MGI
  • spermatogenesis Source: MGI
  • transcription from RNA polymerase II promoter Source: BHF-UCL
  • transdifferentiation Source: Ensembl
  • tricuspid valve formation Source: BHF-UCL
  • tube morphogenesis Source: MGI
  • vasculogenesis involved in coronary vascular morphogenesis Source: DFLAT
  • ventricular cardiac muscle tissue development Source: MGI
  • ventricular septum development Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_280010. Factors involved in megakaryocyte development and platelet production.
REACT_287523. YAP1- and WWTR1 (TAZ)-stimulated gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor GATA-4
Alternative name(s):
GATA-binding factor 4
Gene namesi
Name:Gata4
Synonyms:Gata-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:95664. Gata4.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • nuclear chromatin Source: BHF-UCL
  • nucleus Source: BHF-UCL
  • RNA polymerase II transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441Transcription factor GATA-4PRO_0000083414Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei299 – 2991N6-methyllysine; by EZH21 Publication

Post-translational modificationi

Methylation at Lys-299 attenuates transcriptional activity.1 Publication

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ08369.
PRIDEiQ08369.

PTM databases

PhosphoSiteiQ08369.

Expressioni

Tissue specificityi

Heart, intestine, liver, primative endoderm and gonads.

Inductioni

By retinoic acid.

Gene expression databases

CleanExiMM_GATA4.
ExpressionAtlasiQ08369. baseline and differential.
GenevisibleiQ08369. MM.

Interactioni

Subunit structurei

Interacts with ZNF260 (By similarity). Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with NFATC4 and LMCD1. Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes. Interacts with NR5A1, ZFPM2 and TBX5. Interacts with TBX18.By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Jarid2Q623153EBI-297008,EBI-493592
Nkx2-5P425824EBI-297008,EBI-297021
TBX5Q995932EBI-297008,EBI-297043From a different organism.

Protein-protein interaction databases

BioGridi199841. 18 interactions.
DIPiDIP-33032N.
IntActiQ08369. 3 interactions.
MINTiMINT-206005.
STRINGi10090.ENSMUSP00000113891.

Structurei

3D structure databases

ProteinModelPortaliQ08369.
SMRiQ08369. Positions 212-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi118 – 1269Poly-Ala
Compositional biasi174 – 1818Poly-Ala
Compositional biasi275 – 2795Poly-Thr
Compositional biasi354 – 3596Poly-Ser

Sequence similaritiesi

Contains 2 GATA-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri216 – 24025GATA-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri270 – 29425GATA-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5641.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047700.
HOVERGENiHBG051703.
InParanoidiQ08369.
KOiK09183.
OrthoDBiEOG7CCBRF.
PhylomeDBiQ08369.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR008013. GATA_N.
IPR028436. TF_GATA_4.
IPR016375. TF_GATA_4/5/6.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF154. PTHR10071:SF154. 1 hit.
PfamiPF00320. GATA. 2 hits.
PF05349. GATA-N. 1 hit.
[Graphical view]
PIRSFiPIRSF003028. TF_GATA_4/5/6. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYQSLAMAAN HGPPPGAYEA GGPGAFMHSA GAASSPVYVP TPRVPSSVLG
60 70 80 90 100
LSYLQGGGSA AAAGTTSGGS SGAGPSGAGP GTQQGSPGWS QAGAEGAAYT
110 120 130 140 150
PPPVSPRFSF PGTTGSLAAA AAAAAAREAA AYGSGGGAAG AGLAGREQYG
160 170 180 190 200
RPGFAGSYSS PYPAYMADVG ASWAAAAAAS AGPFDSPVLH SLPGRANPGR
210 220 230 240 250
HPNLDMFDDF SEGRECVNCG AMSTPLWRRD GTGHYLCNAC GLYHKMNGIN
260 270 280 290 300
RPLIKPQRRL SASRRVGLSC ANCQTTTTTL WRRNAEGEPV CNACGLYMKL
310 320 330 340 350
HGVPRPLAMR KEGIQTRKRK PKNLNKSKTP AGPAGETLPP SSGASSGNSS
360 370 380 390 400
NATSSSSSSE EMRPIKTEPG LSSHYGHSSS MSQTFSTVSG HGPSIHPVLS
410 420 430 440
ALKLSPQGYA SPVTQTSQAS SKQDSWNSLV LADSHGDIIT A
Length:441
Mass (Da):44,580
Last modified:March 19, 2014 - v4
Checksum:iE9521771741DD08E
GO

Sequence cautioni

The sequence AAA37662.1 differs from that shown. Reason: Frameshift at positions 6, 40 and 69. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti385 – 40218FSTVS…VLSAL → SVCVRPRALHPSSAVCS in AAA37662 (PubMed:8455608).CuratedAdd
BLAST
Sequence conflicti385 – 40218FSTVS…VLSAL → SVCVRPRALHPSSAVCS in AAB42015 (Ref. 3) CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98339 mRNA. Translation: AAA37662.1. Frameshift.
U85046 mRNA. Translation: AAB42015.1.
AF179424 mRNA. Translation: AAD55266.1.
AC090654 Genomic DNA. No translation available.
AC090962 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36067.1.
BC137824 mRNA. Translation: AAI37825.1.
CCDSiCCDS49519.1.
PIRiA48099.
RefSeqiNP_032118.2. NM_008092.3.
UniGeneiMm.247669.

Genome annotation databases

EnsembliENSMUST00000118022; ENSMUSP00000113891; ENSMUSG00000021944.
GeneIDi14463.
KEGGimmu:14463.
UCSCiuc007uhn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98339 mRNA. Translation: AAA37662.1. Frameshift.
U85046 mRNA. Translation: AAB42015.1.
AF179424 mRNA. Translation: AAD55266.1.
AC090654 Genomic DNA. No translation available.
AC090962 Genomic DNA. No translation available.
CH466535 Genomic DNA. Translation: EDL36067.1.
BC137824 mRNA. Translation: AAI37825.1.
CCDSiCCDS49519.1.
PIRiA48099.
RefSeqiNP_032118.2. NM_008092.3.
UniGeneiMm.247669.

3D structure databases

ProteinModelPortaliQ08369.
SMRiQ08369. Positions 212-320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199841. 18 interactions.
DIPiDIP-33032N.
IntActiQ08369. 3 interactions.
MINTiMINT-206005.
STRINGi10090.ENSMUSP00000113891.

Chemistry

ChEMBLiCHEMBL1687680.

PTM databases

PhosphoSiteiQ08369.

Proteomic databases

PaxDbiQ08369.
PRIDEiQ08369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000118022; ENSMUSP00000113891; ENSMUSG00000021944.
GeneIDi14463.
KEGGimmu:14463.
UCSCiuc007uhn.1. mouse.

Organism-specific databases

CTDi2626.
MGIiMGI:95664. Gata4.

Phylogenomic databases

eggNOGiCOG5641.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047700.
HOVERGENiHBG051703.
InParanoidiQ08369.
KOiK09183.
OrthoDBiEOG7CCBRF.
PhylomeDBiQ08369.

Enzyme and pathway databases

ReactomeiREACT_280010. Factors involved in megakaryocyte development and platelet production.
REACT_287523. YAP1- and WWTR1 (TAZ)-stimulated gene expression.

Miscellaneous databases

NextBioi286104.
PROiQ08369.
SOURCEiSearch...

Gene expression databases

CleanExiMM_GATA4.
ExpressionAtlasiQ08369. baseline and differential.
GenevisibleiQ08369. MM.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR008013. GATA_N.
IPR028436. TF_GATA_4.
IPR016375. TF_GATA_4/5/6.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF154. PTHR10071:SF154. 1 hit.
PfamiPF00320. GATA. 2 hits.
PF05349. GATA-N. 1 hit.
[Graphical view]
PIRSFiPIRSF003028. TF_GATA_4/5/6. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart."
    Arceci R.J., King A.A., Simon M.C., Orkin S.H., Wilson D.B.
    Mol. Cell. Biol. 13:2235-2246(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "GATA-4/5/6, a subfamily of three transcription factors transcribed in developing heart and gut."
    Laverriere A.C., Macneill C., Mueller C., Poelmann R.E., Burch J.B.E., Evans T.
    J. Biol. Chem. 269:23177-23184(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFTS.
  3. "Murine GATA-4 is expressed in heart and gut tissues."
    Morrisey E.E., Parmacek M.S.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. Katsuoka F., Motohashi H., Yamamoto M.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X DBA/2.
    Tissue: Heart.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  8. "Regulation of J6 gene expression by transcription factor GATA-4."
    Bielinska M., Wilson D.B.
    Biochem. J. 307:183-189(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  9. "A calcineurin-dependent transcriptional pathway for cardiac hypertrophy."
    Molkentin J.D., Lu J.-R., Antos C.L., Markham B., Richardson J., Robbins J., Grant S.R., Olson E.N.
    Cell 93:215-228(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NFATC4.
  10. "The cardiac tissue-restricted homeobox protein Csx/Nkx2.5 physically associates with the zinc finger protein GATA4 and cooperatively activates atrial natriuretic factor gene expression."
    Lee Y., Shioi T., Kasahara H., Jobe S.M., Wiese R.J., Markham B.E., Izumo S.
    Mol. Cell. Biol. 18:3120-3129(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NKX2-5.
  11. "Jumonji represses atrial natriuretic factor gene expression by inhibiting transcriptional activities of cardiac transcription factors."
    Kim T.-G., Chen J., Sadoshima J., Lee Y.
    Mol. Cell. Biol. 24:10151-10160(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH JARID2.
  12. "LMCD1/Dyxin is a novel transcriptional cofactor that restricts GATA6 function by inhibiting DNA binding."
    Rath N., Wang Z., Lu M.M., Morrisey E.E.
    Mol. Cell. Biol. 25:8864-8873(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LMCD1.
  13. "Transcriptional repression by the T-box proteins Tbx18 and Tbx15 depends on Groucho corepressors."
    Farin H.F., Bussen M., Schmidt M.K., Singh M.K., Schuster-Gossler K., Kispert A.
    J. Biol. Chem. 282:25748-25759(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TBX18.
  14. "PRC2 directly methylates GATA4 and represses its transcriptional activity."
    He A., Shen X., Ma Q., Cao J., von Gise A., Zhou P., Wang G., Marquez V.E., Orkin S.H., Pu W.T.
    Genes Dev. 26:37-42(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT LYS-299.

Entry informationi

Entry nameiGATA4_MOUSE
AccessioniPrimary (citable) accession number: Q08369
Secondary accession number(s): B9EHF7, P97491, Q9QZK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: March 19, 2014
Last modified: July 22, 2015
This is version 140 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.