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Q08358

- PP220_ASFB7

UniProt

Q08358 - PP220_ASFB7

Protein

Polyprotein pp220

Gene

Ba71V-92

Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Polyprotein pp220 is essential for the core assembly. Its myristoyl moiety may function as a membrane-anchoring signal to bind the developing core shell to the inner viral envelope.
    The structural protein p34 is a component of the virus core shell.
    The structural protein p14 is a component of the virus core shell.
    The structural protein p37 is a component of the virus core shell.
    The structural protein p150 is a component of the virus core shell.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei45 – 462Cleavage; by viral protease S273RSequence Analysis
    Sitei369 – 3702Cleavage; by viral protease S273RSequence Analysis
    Sitei523 – 5242Cleavage; by viral protease S273RSequence Analysis
    Sitei894 – 8952Cleavage; by viral protease S273RSequence Analysis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyprotein pp220
    Alternative name(s):
    220 kDa polyprotein
    Cleaved into the following 4 chains:
    Gene namesi
    Ordered Locus Names:Ba71V-92
    ORF Names:CP2475L
    OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
    Taxonomic identifieri10498 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
    Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000000624: Genome

    Subcellular locationi

    Chain Polyprotein pp220 : Host cytoplasmhost perinuclear region
    Note: Found in perinuclear cytoplasmic viral factories during assembly.
    Chain p34 : Virion Curated. Host cytoplasmhost perinuclear region 1 Publication
    Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.
    Chain p14 : Virion Curated. Host cytoplasmhost perinuclear region
    Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.
    Chain p37 : Virion Curated. Host cytoplasmhost perinuclear region 1 Publication. Host nucleus 1 Publication
    Note: Nuclear at early stages of infection. Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.
    Chain p150 : Virion Curated. Host cytoplasmhost perinuclear region 1 Publication
    Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
    3. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed; by host
    Propeptidei2 – 4544PRO_0000036722Add
    BLAST
    Chaini46 – 894849Polyprotein pp220Sequence AnalysisPRO_0000355531Add
    BLAST
    Chaini46 – 369324p34Sequence AnalysisPRO_0000036723Add
    BLAST
    Chaini370 – 523154p14Sequence AnalysisPRO_0000036724Add
    BLAST
    Chaini524 – 894371p37Sequence AnalysisPRO_0000036725Add
    BLAST
    Chaini895 – 24751581p150Sequence AnalysisPRO_0000036726Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine; by host1 Publication

    Post-translational modificationi

    The polyprotein is not glycosylated.
    Specific enzymatic cleavages in vivo yield mature proteins.1 Publication

    Keywords - PTMi

    Lipoprotein, Myristate

    Expressioni

    Inductioni

    Expressed late in the infection.

    Keywords - Developmental stagei

    Late protein

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili2185 – 221228Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi551 – 5544Poly-Tyr

    Sequence similaritiesi

    Belongs to the asfivirus polyprotein pp220 family.Curated

    Keywords - Domaini

    Coiled coil

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q08358-1 [UniParc]FASTAAdd to Basket

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    MGNRGSSTSS RPPLSSEANL YAKLQDHIQR QTRPFSGGGY FNGGGDKNPV     50
    QHIKDYHIDS VSSKAKLRVI EGIIRAIAKI GFKVDTKQPI EDILKDIKKQ 100
    LPDPRAGSTF VKNAEKQETV CKMIADAINQ EFIDLGQDKL IDTTDGAASI 150
    CRQIVLYINS LTHGLRAEYL DVHGSIENTL ENIKLLNDAI KQLHERMVTE 200
    VTKAAPNEEV INAVTMIEAV YRRLLNEQNL QINILTNFID NILTPTQKEL 250
    DKLQTDEVDI IKLLNDTNSV LGTKNFGKVL SYTLCNLGIA ASVANKINKA 300
    LQKVGLKVEQ YLQSKNWAEF DKELDLKRFS GLVSAENIAE FEKAVNLLRQ 350
    TFNERHKILE NSCAKKGGDE EKTPLDRRIE AQRLDRKHIL MEFLNKSTQA 400
    YNDFLENVKK IGIKLVKEIA LTPNITRLRD ALSRINDMGT IALDLSLIGF 450
    YTNAAAREER ETFLTQFMLV KNVLEEQSKI DPNFKNLYDS CSRLLQIIDF 500
    YTDIVQKKYG GGEDCECTRV GGAALTVEEL GLSKAARSQV DLNQAINTFM 550
    YYYYVAQIYS NLTHNKQEFQ SYEENYATIL GDAIAGRLMQ LDTEKNARIN 600
    SPAVDLARGH VGPNPGGAQE ADWKAAVSAI ELEYDVKRRF YRALEGLDLY 650
    LKNITKTFVN NIDSIQTVQQ MLDGVRIIGR WFTEATGDTL AQVFESFPTS 700
    AGNDSNVFTD NAPAGHYYEK VAAEIQQGRS VGTLRPVRAS QAKNIRDLIG 750
    RSLSNFQALK NIINAFARIG DMLGGEELRQ MVPMSPLQIY KTLLEYIQHS 800
    ALSVGLKNLN QSEIGGQRVA LARTPEEAAQ RVYLSTVRVN DALSTRWETE 850
    DVFFTFMLKS MAAKIFIVLG IYDMFERPEP VYKLIPTRMI LGGADELEPE 900
    VIPEAAELYF RLPRLAEFYQ KLFSFRDENV QISMLPELEG IFSGLIRIIF 950
    MRPIELINIG DYSETEIRQL IKEINVIYQH FNLEYGEQEA TKKALIHFVN 1000
    EINRRFGVIT RTEWEKFQRI VQEARTMNDF GMMNQTNYSI LPDEDGYTQS 1050
    SQLLPSDRFI SPSTQPTPKW RPALYNIDSV DVQTGMLQPN SQWDLVQKFR 1100
    KQLSEMFEDP SLQQELGKVS YQELIRQAIN ELKKEHTDKI QIVSKLIQGS 1150
    ESLADTDVNK IFLFHETVIT GLNLLSAIYV LLNNFRNNIK GLDLDTIQKS 1200
    IIEWLRETQA ANVNRANLID WLGRKHGAIS EIRNPGLVVK ENDVRLSRVY 1250
    PDPTTNATAP QDQNLVTETL FAWIVPYVGI PAGGGVRAEQ ELAARYLVDN 1300
    QRIMQLLLTN IFEMTSSFNK MVQVRFPETS TAQVHLDFTG LISLIDSLMA 1350
    DTKYFLNLLR PHIDKNIIQY YENRSNPGSF YWLEEHLIDK LIKPPTDAGG 1400
    RPLPGGELGL EGVNQIINKT YILLTKPYNV LQLRGGVQRR DAANIQINNN 1450
    PQPSERFEQY GRVFSRLVFY DALENNSGLR VEQVVLGDFR LSNLIRTNNA 1500
    QEENTLSYWD NMAPRTYANV NDAANNLRRY RLYGSDYGIQ NNRSMMMVFN 1550
    QLVASYIARF YDAPSGKIYL NLINAFANGN FSQAVMELGY THPDLARDNI 1600
    AFGHRGDPTE QSVLLLSLGL MLQRLIKDTN RQGLSQHLIS TLTEIPIYLK 1650
    ENYRANLPLF NKMFNILISQ GELLKQFIQY TNVQLARPNL MGLLGANNDS 1700
    VIYYNNNINV PMTGLSVGQA ALRGIGGVFR PNVTLMPLGD AQNNTSDVVR 1750
    KRLVAVIDGI IRGSHTLADS AMEVLHELTD HPIYLETEEH FIQNYMSRYN 1800
    KEPLMPFSLS LYYLRDLRIE NNEVYDPLLY PNLESGSPEF KLLYGTRKLL 1850
    GNDPVQLSDM PGVQLIMKNY NETVVAREQI TPTRFEHFYT HAIQALRFIV 1900
    NIRSFKTVMM YNENTFGGVN LISENRDDKP IITAGIGMNA VYSLRKTLQD 1950
    VISFVESSYQ EEQINHIHKI VSPKGQTRTL GSNRERERIF NLFDMNIIPI 2000
    NVNALMRSIP LANIYNYDYS FEEIACLMYG ISAEKVRSLN TAAPQPDIAE 2050
    VLNIPNRPPM NTREFMLKLL INPYVSVSIT QYGNELMSKG SAGYMSRIFR 2100
    GDNALNMGRP KFLSDQIFNK VLFGSLYPTQ FDYDEAGPGL AAGIQRGREQ 2150
    WGQPLSEYIN QALHELVRTI RIPQKLRVLR NIIVKNQLIA DLTTIREQLV 2200
    SMRREVENMI QTPEIQNNPT PEVIAAAQNW TQQYRARVDT LINFIGNIGQ 2250
    PNSMLDLIQT ITPVTVRAQL GVIFNRHGIP VPHPRQILQT DDEATQWFMT 2300
    NILNIPAIIM TPFTDLANDL RTFLETLERY VFNVPRWLGP STGRVARAPV 2350
    RMAPRDMRHP ISYTENSVLT YITEQNREEG PWSIVKQVGV GIQKPTLVQI 2400
    GKDRFDTRLI RNLIFITNIQ RLLRLRLNLE LSQFRNVLVS PDHIINPSIT 2450
    EYGFSITGPS ETFSDKQYDS DIRIL 2475
    Length:2,475
    Mass (Da):281,482
    Last modified:January 23, 2007 - v3
    Checksum:i5D0415A506BE218E
    GO

    Sequence cautioni

    The sequence CAA61203.1 differs from that shown. Reason: Frameshift at position 713.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22777 Genomic DNA. Translation: CAA80455.1.
    U18466 Genomic DNA. Translation: AAA65321.1.
    X87965 Genomic DNA. Translation: CAA61203.1. Frameshift.
    PIRiS35307.
    RefSeqiNP_042785.1. NC_001659.1.

    Genome annotation databases

    GeneIDi1488856.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22777 Genomic DNA. Translation: CAA80455.1 .
    U18466 Genomic DNA. Translation: AAA65321.1 .
    X87965 Genomic DNA. Translation: CAA61203.1 . Frameshift.
    PIRi S35307.
    RefSeqi NP_042785.1. NC_001659.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1488856.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Polyprotein processing in African swine fever virus: a novel gene expression strategy for a DNA virus."
      Simon-Mateo C., Andres G., Vinuela E.
      EMBO J. 12:2977-2987(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEOLYTIC PROCESSING OF POLYPROTEIN, MYRISTOYLATION AT GLY-2.
    2. "Analysis of the complete nucleotide sequence of African swine fever virus."
      Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
      Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Mapping and sequence of the gene encoding protein p37, a major structural protein of African swine fever virus."
      Lopez-Otin C., Simon C., Mendez E., Vinuela E.
      Virus Genes 1:291-303(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 391-808.
    4. "Assembly of African swine fever virus: role of polyprotein pp220."
      Andres G., Simon-Mateo C., Vinuela E.
      J. Virol. 71:2331-2341(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION OF POLYPROTEIN PP220.
    5. "Repression of African swine fever virus polyprotein pp220-encoding gene leads to the assembly of icosahedral core-less particles."
      Andres G., Garcia-Escudero R., Salas M.L., Rodriguez J.M.
      J. Virol. 76:2654-2666(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF POLYPROTEIN PP220.
    6. "African swine fever virus polyproteins pp220 and pp62 assemble into the core shell."
      Andres G., Alejo A., Salas J., Salas M.L.
      J. Virol. 76:12473-12482(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "Membrane association facilitates the correct processing of pp220 during production of the major matrix proteins of African swine fever virus."
      Heath C.M., Windsor M., Wileman T.
      J. Virol. 77:1682-1690(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION OF P150 AND P34.
    8. "African swine fever virus p37 structural protein is localized in nuclear foci containing the viral DNA at early post-infection times."
      Eulalio A., Nunes-Correia I., Salas J., Salas M.L., Simoes S., Pedroso de Lima M.C.
      Virus Res. 130:18-27(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION OF P37.

    Entry informationi

    Entry nameiPP220_ASFB7
    AccessioniPrimary (citable) accession number: Q08358
    Secondary accession number(s): Q65287
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 59 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3