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Q08358

- PP220_ASFB7

UniProt

Q08358 - PP220_ASFB7

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Protein

Polyprotein pp220

Gene

Ba71V-92

Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Polyprotein pp220 is essential for the core assembly. Its myristoyl moiety may function as a membrane-anchoring signal to bind the developing core shell to the inner viral envelope.
The structural protein p34 is a component of the virus core shell.
The structural protein p14 is a component of the virus core shell.
The structural protein p37 is a component of the virus core shell.
The structural protein p150 is a component of the virus core shell.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei45 – 462Cleavage; by viral protease S273RSequence Analysis
Sitei369 – 3702Cleavage; by viral protease S273RSequence Analysis
Sitei523 – 5242Cleavage; by viral protease S273RSequence Analysis
Sitei894 – 8952Cleavage; by viral protease S273RSequence Analysis

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprotein pp220
Alternative name(s):
220 kDa polyprotein
Cleaved into the following 4 chains:
Gene namesi
Ordered Locus Names:Ba71V-92
ORF Names:CP2475L
OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Taxonomic identifieri10498 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000624: Genome

Subcellular locationi

Chain Polyprotein pp220 : Host cytoplasmhost perinuclear region
Note: Found in perinuclear cytoplasmic viral factories during assembly.
Chain p34 : Virion Curated. Host cytoplasmhost perinuclear region 1 Publication
Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.
Chain p14 : Virion Curated. Host cytoplasmhost perinuclear region
Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.
Chain p37 : Virion Curated. Host cytoplasmhost perinuclear region 1 Publication. Host nucleus 1 Publication
Note: Nuclear at early stages of infection. Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.
Chain p150 : Virion Curated. Host cytoplasmhost perinuclear region 1 Publication
Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by host
Propeptidei2 – 4544PRO_0000036722Add
BLAST
Chaini46 – 894849Polyprotein pp220Sequence AnalysisPRO_0000355531Add
BLAST
Chaini46 – 369324p34Sequence AnalysisPRO_0000036723Add
BLAST
Chaini370 – 523154p14Sequence AnalysisPRO_0000036724Add
BLAST
Chaini524 – 894371p37Sequence AnalysisPRO_0000036725Add
BLAST
Chaini895 – 24751581p150Sequence AnalysisPRO_0000036726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by host1 Publication

Post-translational modificationi

The polyprotein is not glycosylated.
Specific enzymatic cleavages in vivo yield mature proteins.1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Expressioni

Inductioni

Expressed late in the infection.

Keywords - Developmental stagei

Late protein

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili2185 – 221228Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi551 – 5544Poly-Tyr

Sequence similaritiesi

Belongs to the asfivirus polyprotein pp220 family.Curated

Keywords - Domaini

Coiled coil

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08358-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MGNRGSSTSS RPPLSSEANL YAKLQDHIQR QTRPFSGGGY FNGGGDKNPV
60 70 80 90 100
QHIKDYHIDS VSSKAKLRVI EGIIRAIAKI GFKVDTKQPI EDILKDIKKQ
110 120 130 140 150
LPDPRAGSTF VKNAEKQETV CKMIADAINQ EFIDLGQDKL IDTTDGAASI
160 170 180 190 200
CRQIVLYINS LTHGLRAEYL DVHGSIENTL ENIKLLNDAI KQLHERMVTE
210 220 230 240 250
VTKAAPNEEV INAVTMIEAV YRRLLNEQNL QINILTNFID NILTPTQKEL
260 270 280 290 300
DKLQTDEVDI IKLLNDTNSV LGTKNFGKVL SYTLCNLGIA ASVANKINKA
310 320 330 340 350
LQKVGLKVEQ YLQSKNWAEF DKELDLKRFS GLVSAENIAE FEKAVNLLRQ
360 370 380 390 400
TFNERHKILE NSCAKKGGDE EKTPLDRRIE AQRLDRKHIL MEFLNKSTQA
410 420 430 440 450
YNDFLENVKK IGIKLVKEIA LTPNITRLRD ALSRINDMGT IALDLSLIGF
460 470 480 490 500
YTNAAAREER ETFLTQFMLV KNVLEEQSKI DPNFKNLYDS CSRLLQIIDF
510 520 530 540 550
YTDIVQKKYG GGEDCECTRV GGAALTVEEL GLSKAARSQV DLNQAINTFM
560 570 580 590 600
YYYYVAQIYS NLTHNKQEFQ SYEENYATIL GDAIAGRLMQ LDTEKNARIN
610 620 630 640 650
SPAVDLARGH VGPNPGGAQE ADWKAAVSAI ELEYDVKRRF YRALEGLDLY
660 670 680 690 700
LKNITKTFVN NIDSIQTVQQ MLDGVRIIGR WFTEATGDTL AQVFESFPTS
710 720 730 740 750
AGNDSNVFTD NAPAGHYYEK VAAEIQQGRS VGTLRPVRAS QAKNIRDLIG
760 770 780 790 800
RSLSNFQALK NIINAFARIG DMLGGEELRQ MVPMSPLQIY KTLLEYIQHS
810 820 830 840 850
ALSVGLKNLN QSEIGGQRVA LARTPEEAAQ RVYLSTVRVN DALSTRWETE
860 870 880 890 900
DVFFTFMLKS MAAKIFIVLG IYDMFERPEP VYKLIPTRMI LGGADELEPE
910 920 930 940 950
VIPEAAELYF RLPRLAEFYQ KLFSFRDENV QISMLPELEG IFSGLIRIIF
960 970 980 990 1000
MRPIELINIG DYSETEIRQL IKEINVIYQH FNLEYGEQEA TKKALIHFVN
1010 1020 1030 1040 1050
EINRRFGVIT RTEWEKFQRI VQEARTMNDF GMMNQTNYSI LPDEDGYTQS
1060 1070 1080 1090 1100
SQLLPSDRFI SPSTQPTPKW RPALYNIDSV DVQTGMLQPN SQWDLVQKFR
1110 1120 1130 1140 1150
KQLSEMFEDP SLQQELGKVS YQELIRQAIN ELKKEHTDKI QIVSKLIQGS
1160 1170 1180 1190 1200
ESLADTDVNK IFLFHETVIT GLNLLSAIYV LLNNFRNNIK GLDLDTIQKS
1210 1220 1230 1240 1250
IIEWLRETQA ANVNRANLID WLGRKHGAIS EIRNPGLVVK ENDVRLSRVY
1260 1270 1280 1290 1300
PDPTTNATAP QDQNLVTETL FAWIVPYVGI PAGGGVRAEQ ELAARYLVDN
1310 1320 1330 1340 1350
QRIMQLLLTN IFEMTSSFNK MVQVRFPETS TAQVHLDFTG LISLIDSLMA
1360 1370 1380 1390 1400
DTKYFLNLLR PHIDKNIIQY YENRSNPGSF YWLEEHLIDK LIKPPTDAGG
1410 1420 1430 1440 1450
RPLPGGELGL EGVNQIINKT YILLTKPYNV LQLRGGVQRR DAANIQINNN
1460 1470 1480 1490 1500
PQPSERFEQY GRVFSRLVFY DALENNSGLR VEQVVLGDFR LSNLIRTNNA
1510 1520 1530 1540 1550
QEENTLSYWD NMAPRTYANV NDAANNLRRY RLYGSDYGIQ NNRSMMMVFN
1560 1570 1580 1590 1600
QLVASYIARF YDAPSGKIYL NLINAFANGN FSQAVMELGY THPDLARDNI
1610 1620 1630 1640 1650
AFGHRGDPTE QSVLLLSLGL MLQRLIKDTN RQGLSQHLIS TLTEIPIYLK
1660 1670 1680 1690 1700
ENYRANLPLF NKMFNILISQ GELLKQFIQY TNVQLARPNL MGLLGANNDS
1710 1720 1730 1740 1750
VIYYNNNINV PMTGLSVGQA ALRGIGGVFR PNVTLMPLGD AQNNTSDVVR
1760 1770 1780 1790 1800
KRLVAVIDGI IRGSHTLADS AMEVLHELTD HPIYLETEEH FIQNYMSRYN
1810 1820 1830 1840 1850
KEPLMPFSLS LYYLRDLRIE NNEVYDPLLY PNLESGSPEF KLLYGTRKLL
1860 1870 1880 1890 1900
GNDPVQLSDM PGVQLIMKNY NETVVAREQI TPTRFEHFYT HAIQALRFIV
1910 1920 1930 1940 1950
NIRSFKTVMM YNENTFGGVN LISENRDDKP IITAGIGMNA VYSLRKTLQD
1960 1970 1980 1990 2000
VISFVESSYQ EEQINHIHKI VSPKGQTRTL GSNRERERIF NLFDMNIIPI
2010 2020 2030 2040 2050
NVNALMRSIP LANIYNYDYS FEEIACLMYG ISAEKVRSLN TAAPQPDIAE
2060 2070 2080 2090 2100
VLNIPNRPPM NTREFMLKLL INPYVSVSIT QYGNELMSKG SAGYMSRIFR
2110 2120 2130 2140 2150
GDNALNMGRP KFLSDQIFNK VLFGSLYPTQ FDYDEAGPGL AAGIQRGREQ
2160 2170 2180 2190 2200
WGQPLSEYIN QALHELVRTI RIPQKLRVLR NIIVKNQLIA DLTTIREQLV
2210 2220 2230 2240 2250
SMRREVENMI QTPEIQNNPT PEVIAAAQNW TQQYRARVDT LINFIGNIGQ
2260 2270 2280 2290 2300
PNSMLDLIQT ITPVTVRAQL GVIFNRHGIP VPHPRQILQT DDEATQWFMT
2310 2320 2330 2340 2350
NILNIPAIIM TPFTDLANDL RTFLETLERY VFNVPRWLGP STGRVARAPV
2360 2370 2380 2390 2400
RMAPRDMRHP ISYTENSVLT YITEQNREEG PWSIVKQVGV GIQKPTLVQI
2410 2420 2430 2440 2450
GKDRFDTRLI RNLIFITNIQ RLLRLRLNLE LSQFRNVLVS PDHIINPSIT
2460 2470
EYGFSITGPS ETFSDKQYDS DIRIL
Length:2,475
Mass (Da):281,482
Last modified:January 23, 2007 - v3
Checksum:i5D0415A506BE218E
GO

Sequence cautioni

The sequence CAA61203.1 differs from that shown. Reason: Frameshift at position 713. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22777 Genomic DNA. Translation: CAA80455.1.
U18466 Genomic DNA. Translation: AAA65321.1.
X87965 Genomic DNA. Translation: CAA61203.1. Frameshift.
PIRiS35307.
RefSeqiNP_042785.1. NC_001659.1.

Genome annotation databases

GeneIDi1488856.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22777 Genomic DNA. Translation: CAA80455.1 .
U18466 Genomic DNA. Translation: AAA65321.1 .
X87965 Genomic DNA. Translation: CAA61203.1 . Frameshift.
PIRi S35307.
RefSeqi NP_042785.1. NC_001659.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1488856.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Polyprotein processing in African swine fever virus: a novel gene expression strategy for a DNA virus."
    Simon-Mateo C., Andres G., Vinuela E.
    EMBO J. 12:2977-2987(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEOLYTIC PROCESSING OF POLYPROTEIN, MYRISTOYLATION AT GLY-2.
  2. "Analysis of the complete nucleotide sequence of African swine fever virus."
    Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
    Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Mapping and sequence of the gene encoding protein p37, a major structural protein of African swine fever virus."
    Lopez-Otin C., Simon C., Mendez E., Vinuela E.
    Virus Genes 1:291-303(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 391-808.
  4. "Assembly of African swine fever virus: role of polyprotein pp220."
    Andres G., Simon-Mateo C., Vinuela E.
    J. Virol. 71:2331-2341(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION OF POLYPROTEIN PP220.
  5. "Repression of African swine fever virus polyprotein pp220-encoding gene leads to the assembly of icosahedral core-less particles."
    Andres G., Garcia-Escudero R., Salas M.L., Rodriguez J.M.
    J. Virol. 76:2654-2666(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF POLYPROTEIN PP220.
  6. "African swine fever virus polyproteins pp220 and pp62 assemble into the core shell."
    Andres G., Alejo A., Salas J., Salas M.L.
    J. Virol. 76:12473-12482(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Membrane association facilitates the correct processing of pp220 during production of the major matrix proteins of African swine fever virus."
    Heath C.M., Windsor M., Wileman T.
    J. Virol. 77:1682-1690(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION OF P150 AND P34.
  8. "African swine fever virus p37 structural protein is localized in nuclear foci containing the viral DNA at early post-infection times."
    Eulalio A., Nunes-Correia I., Salas J., Salas M.L., Simoes S., Pedroso de Lima M.C.
    Virus Res. 130:18-27(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION OF P37.

Entry informationi

Entry nameiPP220_ASFB7
AccessioniPrimary (citable) accession number: Q08358
Secondary accession number(s): Q65287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 60 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3