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Q08358

- PP220_ASFB7

UniProt

Q08358 - PP220_ASFB7

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Protein

Polyprotein pp220

Gene
Ba71V-92, CP2475L
Organism
African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Polyprotein pp220 is essential for the core assembly. Its myristoyl moiety may function as a membrane-anchoring signal to bind the developing core shell to the inner viral envelope.2 Publications
The structural protein p34 is a component of the virus core shell.2 Publications
The structural protein p14 is a component of the virus core shell.2 Publications
The structural protein p37 is a component of the virus core shell.2 Publications
The structural protein p150 is a component of the virus core shell.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei45 – 462Cleavage; by viral protease S273R Reviewed prediction
Sitei369 – 3702Cleavage; by viral protease S273R Reviewed prediction
Sitei523 – 5242Cleavage; by viral protease S273R Reviewed prediction
Sitei894 – 8952Cleavage; by viral protease S273R Reviewed prediction

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprotein pp220
Alternative name(s):
220 kDa polyprotein
Cleaved into the following 4 chains:
Gene namesi
Ordered Locus Names:Ba71V-92
ORF Names:CP2475L
OrganismiAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV)
Taxonomic identifieri10498 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000624: Genome

Subcellular locationi

Chain Polyprotein pp220 : Host cytoplasmhost perinuclear region
Note: Found in perinuclear cytoplasmic viral factories during assembly.4 Publications
Chain p34 : Virion Reviewed prediction. Host cytoplasmhost perinuclear region
Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.4 Publications
Chain p14 : Virion Reviewed prediction. Host cytoplasmhost perinuclear region
Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.4 Publications
Chain p37 : Virion Reviewed prediction. Host cytoplasmhost perinuclear region. Host nucleus
Note: Nuclear at early stages of infection. Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.4 Publications
Chain p150 : Virion Reviewed prediction. Host cytoplasmhost perinuclear region
Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope.4 Publications

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
  2. host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  3. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by host
Propeptidei2 – 4544PRO_0000036722Add
BLAST
Chaini46 – 894849Polyprotein pp220 Reviewed predictionPRO_0000355531Add
BLAST
Chaini46 – 369324p34 Reviewed predictionPRO_0000036723Add
BLAST
Chaini370 – 523154p14 Reviewed predictionPRO_0000036724Add
BLAST
Chaini524 – 894371p37 Reviewed predictionPRO_0000036725Add
BLAST
Chaini895 – 24751581p150 Reviewed predictionPRO_0000036726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by host1 Publication

Post-translational modificationi

The polyprotein is not glycosylated.
Specific enzymatic cleavages in vivo yield mature proteins.

Keywords - PTMi

Lipoprotein, Myristate

Expressioni

Inductioni

Expressed late in the infection.

Keywords - Developmental stagei

Late protein

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili2185 – 221228 Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi551 – 5544Poly-Tyr

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08358-1 [UniParc]FASTAAdd to Basket

« Hide

MGNRGSSTSS RPPLSSEANL YAKLQDHIQR QTRPFSGGGY FNGGGDKNPV     50
QHIKDYHIDS VSSKAKLRVI EGIIRAIAKI GFKVDTKQPI EDILKDIKKQ 100
LPDPRAGSTF VKNAEKQETV CKMIADAINQ EFIDLGQDKL IDTTDGAASI 150
CRQIVLYINS LTHGLRAEYL DVHGSIENTL ENIKLLNDAI KQLHERMVTE 200
VTKAAPNEEV INAVTMIEAV YRRLLNEQNL QINILTNFID NILTPTQKEL 250
DKLQTDEVDI IKLLNDTNSV LGTKNFGKVL SYTLCNLGIA ASVANKINKA 300
LQKVGLKVEQ YLQSKNWAEF DKELDLKRFS GLVSAENIAE FEKAVNLLRQ 350
TFNERHKILE NSCAKKGGDE EKTPLDRRIE AQRLDRKHIL MEFLNKSTQA 400
YNDFLENVKK IGIKLVKEIA LTPNITRLRD ALSRINDMGT IALDLSLIGF 450
YTNAAAREER ETFLTQFMLV KNVLEEQSKI DPNFKNLYDS CSRLLQIIDF 500
YTDIVQKKYG GGEDCECTRV GGAALTVEEL GLSKAARSQV DLNQAINTFM 550
YYYYVAQIYS NLTHNKQEFQ SYEENYATIL GDAIAGRLMQ LDTEKNARIN 600
SPAVDLARGH VGPNPGGAQE ADWKAAVSAI ELEYDVKRRF YRALEGLDLY 650
LKNITKTFVN NIDSIQTVQQ MLDGVRIIGR WFTEATGDTL AQVFESFPTS 700
AGNDSNVFTD NAPAGHYYEK VAAEIQQGRS VGTLRPVRAS QAKNIRDLIG 750
RSLSNFQALK NIINAFARIG DMLGGEELRQ MVPMSPLQIY KTLLEYIQHS 800
ALSVGLKNLN QSEIGGQRVA LARTPEEAAQ RVYLSTVRVN DALSTRWETE 850
DVFFTFMLKS MAAKIFIVLG IYDMFERPEP VYKLIPTRMI LGGADELEPE 900
VIPEAAELYF RLPRLAEFYQ KLFSFRDENV QISMLPELEG IFSGLIRIIF 950
MRPIELINIG DYSETEIRQL IKEINVIYQH FNLEYGEQEA TKKALIHFVN 1000
EINRRFGVIT RTEWEKFQRI VQEARTMNDF GMMNQTNYSI LPDEDGYTQS 1050
SQLLPSDRFI SPSTQPTPKW RPALYNIDSV DVQTGMLQPN SQWDLVQKFR 1100
KQLSEMFEDP SLQQELGKVS YQELIRQAIN ELKKEHTDKI QIVSKLIQGS 1150
ESLADTDVNK IFLFHETVIT GLNLLSAIYV LLNNFRNNIK GLDLDTIQKS 1200
IIEWLRETQA ANVNRANLID WLGRKHGAIS EIRNPGLVVK ENDVRLSRVY 1250
PDPTTNATAP QDQNLVTETL FAWIVPYVGI PAGGGVRAEQ ELAARYLVDN 1300
QRIMQLLLTN IFEMTSSFNK MVQVRFPETS TAQVHLDFTG LISLIDSLMA 1350
DTKYFLNLLR PHIDKNIIQY YENRSNPGSF YWLEEHLIDK LIKPPTDAGG 1400
RPLPGGELGL EGVNQIINKT YILLTKPYNV LQLRGGVQRR DAANIQINNN 1450
PQPSERFEQY GRVFSRLVFY DALENNSGLR VEQVVLGDFR LSNLIRTNNA 1500
QEENTLSYWD NMAPRTYANV NDAANNLRRY RLYGSDYGIQ NNRSMMMVFN 1550
QLVASYIARF YDAPSGKIYL NLINAFANGN FSQAVMELGY THPDLARDNI 1600
AFGHRGDPTE QSVLLLSLGL MLQRLIKDTN RQGLSQHLIS TLTEIPIYLK 1650
ENYRANLPLF NKMFNILISQ GELLKQFIQY TNVQLARPNL MGLLGANNDS 1700
VIYYNNNINV PMTGLSVGQA ALRGIGGVFR PNVTLMPLGD AQNNTSDVVR 1750
KRLVAVIDGI IRGSHTLADS AMEVLHELTD HPIYLETEEH FIQNYMSRYN 1800
KEPLMPFSLS LYYLRDLRIE NNEVYDPLLY PNLESGSPEF KLLYGTRKLL 1850
GNDPVQLSDM PGVQLIMKNY NETVVAREQI TPTRFEHFYT HAIQALRFIV 1900
NIRSFKTVMM YNENTFGGVN LISENRDDKP IITAGIGMNA VYSLRKTLQD 1950
VISFVESSYQ EEQINHIHKI VSPKGQTRTL GSNRERERIF NLFDMNIIPI 2000
NVNALMRSIP LANIYNYDYS FEEIACLMYG ISAEKVRSLN TAAPQPDIAE 2050
VLNIPNRPPM NTREFMLKLL INPYVSVSIT QYGNELMSKG SAGYMSRIFR 2100
GDNALNMGRP KFLSDQIFNK VLFGSLYPTQ FDYDEAGPGL AAGIQRGREQ 2150
WGQPLSEYIN QALHELVRTI RIPQKLRVLR NIIVKNQLIA DLTTIREQLV 2200
SMRREVENMI QTPEIQNNPT PEVIAAAQNW TQQYRARVDT LINFIGNIGQ 2250
PNSMLDLIQT ITPVTVRAQL GVIFNRHGIP VPHPRQILQT DDEATQWFMT 2300
NILNIPAIIM TPFTDLANDL RTFLETLERY VFNVPRWLGP STGRVARAPV 2350
RMAPRDMRHP ISYTENSVLT YITEQNREEG PWSIVKQVGV GIQKPTLVQI 2400
GKDRFDTRLI RNLIFITNIQ RLLRLRLNLE LSQFRNVLVS PDHIINPSIT 2450
EYGFSITGPS ETFSDKQYDS DIRIL 2475
Length:2,475
Mass (Da):281,482
Last modified:January 23, 2007 - v3
Checksum:i5D0415A506BE218E
GO

Sequence cautioni

The sequence CAA61203.1 differs from that shown. Reason: Frameshift at position 713.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z22777 Genomic DNA. Translation: CAA80455.1.
U18466 Genomic DNA. Translation: AAA65321.1.
X87965 Genomic DNA. Translation: CAA61203.1. Frameshift.
PIRiS35307.
RefSeqiNP_042785.1. NC_001659.1.

Genome annotation databases

GeneIDi1488856.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z22777 Genomic DNA. Translation: CAA80455.1 .
U18466 Genomic DNA. Translation: AAA65321.1 .
X87965 Genomic DNA. Translation: CAA61203.1 . Frameshift.
PIRi S35307.
RefSeqi NP_042785.1. NC_001659.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1488856.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Polyprotein processing in African swine fever virus: a novel gene expression strategy for a DNA virus."
    Simon-Mateo C., Andres G., Vinuela E.
    EMBO J. 12:2977-2987(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEOLYTIC PROCESSING OF POLYPROTEIN, MYRISTOYLATION AT GLY-2.
  2. "Analysis of the complete nucleotide sequence of African swine fever virus."
    Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
    Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Mapping and sequence of the gene encoding protein p37, a major structural protein of African swine fever virus."
    Lopez-Otin C., Simon C., Mendez E., Vinuela E.
    Virus Genes 1:291-303(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 391-808.
  4. "Assembly of African swine fever virus: role of polyprotein pp220."
    Andres G., Simon-Mateo C., Vinuela E.
    J. Virol. 71:2331-2341(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION OF POLYPROTEIN PP220.
  5. "Repression of African swine fever virus polyprotein pp220-encoding gene leads to the assembly of icosahedral core-less particles."
    Andres G., Garcia-Escudero R., Salas M.L., Rodriguez J.M.
    J. Virol. 76:2654-2666(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF POLYPROTEIN PP220.
  6. "African swine fever virus polyproteins pp220 and pp62 assemble into the core shell."
    Andres G., Alejo A., Salas J., Salas M.L.
    J. Virol. 76:12473-12482(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Membrane association facilitates the correct processing of pp220 during production of the major matrix proteins of African swine fever virus."
    Heath C.M., Windsor M., Wileman T.
    J. Virol. 77:1682-1690(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION OF P150 AND P34.
  8. "African swine fever virus p37 structural protein is localized in nuclear foci containing the viral DNA at early post-infection times."
    Eulalio A., Nunes-Correia I., Salas J., Salas M.L., Simoes S., Pedroso de Lima M.C.
    Virus Res. 130:18-27(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION OF P37.

Entry informationi

Entry nameiPP220_ASFB7
AccessioniPrimary (citable) accession number: Q08358
Secondary accession number(s): Q65287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: January 23, 2007
Last modified: April 3, 2013
This is version 58 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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