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Q08358 (PP220_ASFB7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyprotein pp220
Alternative name(s):
220 kDa polyprotein

Cleaved into the following 4 chains:

  1. p34
  2. p14
  3. p37
  4. p150
Gene names
Ordered Locus Names:Ba71V-92
ORF Names:CP2475L
OrganismAfrican swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV) [Reference proteome]
Taxonomic identifier10498 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length2475 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Polyprotein pp220 is essential for the core assembly. Its myristoyl moiety may function as a membrane-anchoring signal to bind the developing core shell to the inner viral envelope. Ref.4 Ref.5

The structural protein p34 is a component of the virus core shell. Ref.4 Ref.5

The structural protein p14 is a component of the virus core shell. Ref.4 Ref.5

The structural protein p37 is a component of the virus core shell. Ref.4 Ref.5

The structural protein p150 is a component of the virus core shell. Ref.4 Ref.5

Subcellular location

Polyprotein pp220: Host cytoplasmhost perinuclear region. Note: Found in perinuclear cytoplasmic viral factories during assembly. Ref.4 Ref.6 Ref.7 Ref.8

p34: Virion Potential. Host cytoplasmhost perinuclear region. Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope. Ref.4 Ref.6 Ref.7 Ref.8

p14: Virion Potential. Host cytoplasmhost perinuclear region. Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope. Ref.4 Ref.6 Ref.7 Ref.8

p37: Virion Potential. Host cytoplasmhost perinuclear region. Host nucleus. Note: Nuclear at early stages of infection. Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope. Ref.4 Ref.6 Ref.7 Ref.8

p150: Virion Potential. Host cytoplasmhost perinuclear region. Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope. Ref.4 Ref.6 Ref.7 Ref.8

Induction

Expressed late in the infection.

Post-translational modification

The polyprotein is not glycosylated.

Specific enzymatic cleavages in vivo yield mature proteins.

Sequence similarities

Belongs to the asfivirus polyprotein pp220 family.

Sequence caution

The sequence CAA61203.1 differs from that shown. Reason: Frameshift at position 713.

Ontologies

Keywords
   Cellular componentHost cytoplasm
Host nucleus
Virion
   Developmental stageLate protein
   DomainCoiled coil
   PTMLipoprotein
Myristate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componenthost cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

virion

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed; by host
Propeptide2 – 4544
PRO_0000036722
Chain46 – 894849Polyprotein pp220 Potential
PRO_0000355531
Chain46 – 369324p34 Potential
PRO_0000036723
Chain370 – 523154p14 Potential
PRO_0000036724
Chain524 – 894371p37 Potential
PRO_0000036725
Chain895 – 24751581p150 Potential
PRO_0000036726

Regions

Coiled coil2185 – 221228 Potential
Compositional bias551 – 5544Poly-Tyr

Sites

Site45 – 462Cleavage; by viral protease S273R Potential
Site369 – 3702Cleavage; by viral protease S273R Potential
Site523 – 5242Cleavage; by viral protease S273R Potential
Site894 – 8952Cleavage; by viral protease S273R Potential

Amino acid modifications

Lipidation21N-myristoyl glycine; by host Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q08358 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 5D0415A506BE218E

FASTA2,475281,482
        10         20         30         40         50         60 
MGNRGSSTSS RPPLSSEANL YAKLQDHIQR QTRPFSGGGY FNGGGDKNPV QHIKDYHIDS 

        70         80         90        100        110        120 
VSSKAKLRVI EGIIRAIAKI GFKVDTKQPI EDILKDIKKQ LPDPRAGSTF VKNAEKQETV 

       130        140        150        160        170        180 
CKMIADAINQ EFIDLGQDKL IDTTDGAASI CRQIVLYINS LTHGLRAEYL DVHGSIENTL 

       190        200        210        220        230        240 
ENIKLLNDAI KQLHERMVTE VTKAAPNEEV INAVTMIEAV YRRLLNEQNL QINILTNFID 

       250        260        270        280        290        300 
NILTPTQKEL DKLQTDEVDI IKLLNDTNSV LGTKNFGKVL SYTLCNLGIA ASVANKINKA 

       310        320        330        340        350        360 
LQKVGLKVEQ YLQSKNWAEF DKELDLKRFS GLVSAENIAE FEKAVNLLRQ TFNERHKILE 

       370        380        390        400        410        420 
NSCAKKGGDE EKTPLDRRIE AQRLDRKHIL MEFLNKSTQA YNDFLENVKK IGIKLVKEIA 

       430        440        450        460        470        480 
LTPNITRLRD ALSRINDMGT IALDLSLIGF YTNAAAREER ETFLTQFMLV KNVLEEQSKI 

       490        500        510        520        530        540 
DPNFKNLYDS CSRLLQIIDF YTDIVQKKYG GGEDCECTRV GGAALTVEEL GLSKAARSQV 

       550        560        570        580        590        600 
DLNQAINTFM YYYYVAQIYS NLTHNKQEFQ SYEENYATIL GDAIAGRLMQ LDTEKNARIN 

       610        620        630        640        650        660 
SPAVDLARGH VGPNPGGAQE ADWKAAVSAI ELEYDVKRRF YRALEGLDLY LKNITKTFVN 

       670        680        690        700        710        720 
NIDSIQTVQQ MLDGVRIIGR WFTEATGDTL AQVFESFPTS AGNDSNVFTD NAPAGHYYEK 

       730        740        750        760        770        780 
VAAEIQQGRS VGTLRPVRAS QAKNIRDLIG RSLSNFQALK NIINAFARIG DMLGGEELRQ 

       790        800        810        820        830        840 
MVPMSPLQIY KTLLEYIQHS ALSVGLKNLN QSEIGGQRVA LARTPEEAAQ RVYLSTVRVN 

       850        860        870        880        890        900 
DALSTRWETE DVFFTFMLKS MAAKIFIVLG IYDMFERPEP VYKLIPTRMI LGGADELEPE 

       910        920        930        940        950        960 
VIPEAAELYF RLPRLAEFYQ KLFSFRDENV QISMLPELEG IFSGLIRIIF MRPIELINIG 

       970        980        990       1000       1010       1020 
DYSETEIRQL IKEINVIYQH FNLEYGEQEA TKKALIHFVN EINRRFGVIT RTEWEKFQRI 

      1030       1040       1050       1060       1070       1080 
VQEARTMNDF GMMNQTNYSI LPDEDGYTQS SQLLPSDRFI SPSTQPTPKW RPALYNIDSV 

      1090       1100       1110       1120       1130       1140 
DVQTGMLQPN SQWDLVQKFR KQLSEMFEDP SLQQELGKVS YQELIRQAIN ELKKEHTDKI 

      1150       1160       1170       1180       1190       1200 
QIVSKLIQGS ESLADTDVNK IFLFHETVIT GLNLLSAIYV LLNNFRNNIK GLDLDTIQKS 

      1210       1220       1230       1240       1250       1260 
IIEWLRETQA ANVNRANLID WLGRKHGAIS EIRNPGLVVK ENDVRLSRVY PDPTTNATAP 

      1270       1280       1290       1300       1310       1320 
QDQNLVTETL FAWIVPYVGI PAGGGVRAEQ ELAARYLVDN QRIMQLLLTN IFEMTSSFNK 

      1330       1340       1350       1360       1370       1380 
MVQVRFPETS TAQVHLDFTG LISLIDSLMA DTKYFLNLLR PHIDKNIIQY YENRSNPGSF 

      1390       1400       1410       1420       1430       1440 
YWLEEHLIDK LIKPPTDAGG RPLPGGELGL EGVNQIINKT YILLTKPYNV LQLRGGVQRR 

      1450       1460       1470       1480       1490       1500 
DAANIQINNN PQPSERFEQY GRVFSRLVFY DALENNSGLR VEQVVLGDFR LSNLIRTNNA 

      1510       1520       1530       1540       1550       1560 
QEENTLSYWD NMAPRTYANV NDAANNLRRY RLYGSDYGIQ NNRSMMMVFN QLVASYIARF 

      1570       1580       1590       1600       1610       1620 
YDAPSGKIYL NLINAFANGN FSQAVMELGY THPDLARDNI AFGHRGDPTE QSVLLLSLGL 

      1630       1640       1650       1660       1670       1680 
MLQRLIKDTN RQGLSQHLIS TLTEIPIYLK ENYRANLPLF NKMFNILISQ GELLKQFIQY 

      1690       1700       1710       1720       1730       1740 
TNVQLARPNL MGLLGANNDS VIYYNNNINV PMTGLSVGQA ALRGIGGVFR PNVTLMPLGD 

      1750       1760       1770       1780       1790       1800 
AQNNTSDVVR KRLVAVIDGI IRGSHTLADS AMEVLHELTD HPIYLETEEH FIQNYMSRYN 

      1810       1820       1830       1840       1850       1860 
KEPLMPFSLS LYYLRDLRIE NNEVYDPLLY PNLESGSPEF KLLYGTRKLL GNDPVQLSDM 

      1870       1880       1890       1900       1910       1920 
PGVQLIMKNY NETVVAREQI TPTRFEHFYT HAIQALRFIV NIRSFKTVMM YNENTFGGVN 

      1930       1940       1950       1960       1970       1980 
LISENRDDKP IITAGIGMNA VYSLRKTLQD VISFVESSYQ EEQINHIHKI VSPKGQTRTL 

      1990       2000       2010       2020       2030       2040 
GSNRERERIF NLFDMNIIPI NVNALMRSIP LANIYNYDYS FEEIACLMYG ISAEKVRSLN 

      2050       2060       2070       2080       2090       2100 
TAAPQPDIAE VLNIPNRPPM NTREFMLKLL INPYVSVSIT QYGNELMSKG SAGYMSRIFR 

      2110       2120       2130       2140       2150       2160 
GDNALNMGRP KFLSDQIFNK VLFGSLYPTQ FDYDEAGPGL AAGIQRGREQ WGQPLSEYIN 

      2170       2180       2190       2200       2210       2220 
QALHELVRTI RIPQKLRVLR NIIVKNQLIA DLTTIREQLV SMRREVENMI QTPEIQNNPT 

      2230       2240       2250       2260       2270       2280 
PEVIAAAQNW TQQYRARVDT LINFIGNIGQ PNSMLDLIQT ITPVTVRAQL GVIFNRHGIP 

      2290       2300       2310       2320       2330       2340 
VPHPRQILQT DDEATQWFMT NILNIPAIIM TPFTDLANDL RTFLETLERY VFNVPRWLGP 

      2350       2360       2370       2380       2390       2400 
STGRVARAPV RMAPRDMRHP ISYTENSVLT YITEQNREEG PWSIVKQVGV GIQKPTLVQI 

      2410       2420       2430       2440       2450       2460 
GKDRFDTRLI RNLIFITNIQ RLLRLRLNLE LSQFRNVLVS PDHIINPSIT EYGFSITGPS 

      2470 
ETFSDKQYDS DIRIL 

« Hide

References

« Hide 'large scale' references
[1]"Polyprotein processing in African swine fever virus: a novel gene expression strategy for a DNA virus."
Simon-Mateo C., Andres G., Vinuela E.
EMBO J. 12:2977-2987(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEOLYTIC PROCESSING OF POLYPROTEIN, MYRISTOYLATION AT GLY-2.
[2]"Analysis of the complete nucleotide sequence of African swine fever virus."
Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E.
Virology 208:249-278(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Mapping and sequence of the gene encoding protein p37, a major structural protein of African swine fever virus."
Lopez-Otin C., Simon C., Mendez E., Vinuela E.
Virus Genes 1:291-303(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 391-808.
[4]"Assembly of African swine fever virus: role of polyprotein pp220."
Andres G., Simon-Mateo C., Vinuela E.
J. Virol. 71:2331-2341(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION OF POLYPROTEIN PP220.
[5]"Repression of African swine fever virus polyprotein pp220-encoding gene leads to the assembly of icosahedral core-less particles."
Andres G., Garcia-Escudero R., Salas M.L., Rodriguez J.M.
J. Virol. 76:2654-2666(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF POLYPROTEIN PP220.
[6]"African swine fever virus polyproteins pp220 and pp62 assemble into the core shell."
Andres G., Alejo A., Salas J., Salas M.L.
J. Virol. 76:12473-12482(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"Membrane association facilitates the correct processing of pp220 during production of the major matrix proteins of African swine fever virus."
Heath C.M., Windsor M., Wileman T.
J. Virol. 77:1682-1690(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION OF P150 AND P34.
[8]"African swine fever virus p37 structural protein is localized in nuclear foci containing the viral DNA at early post-infection times."
Eulalio A., Nunes-Correia I., Salas J., Salas M.L., Simoes S., Pedroso de Lima M.C.
Virus Res. 130:18-27(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION OF P37.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z22777 Genomic DNA. Translation: CAA80455.1.
U18466 Genomic DNA. Translation: AAA65321.1.
X87965 Genomic DNA. Translation: CAA61203.1. Frameshift.
PIRS35307.
RefSeqNP_042785.1. NC_001659.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1488856.

Family and domain databases

ProtoNetSearch...

Entry information

Entry namePP220_ASFB7
AccessionPrimary (citable) accession number: Q08358
Secondary accession number(s): Q65287
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: January 23, 2007
Last modified: April 3, 2013
This is version 58 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families