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Reviewed, UniProtKB/Swiss-Prot Q08352 (DHA_BACSU)

Last modified November 3, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alanine dehydrogenase
    EC=1.4.1.1
Alternative name(s):
    Stage V sporulation protein N
Gene names
Name: ald
Synonyms: spoVN
Ordered Locus Names: BSU31930
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length378 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

This enzyme is a key factor in the assimilation of L-alanine as an energy source through the tricarboxylic acid cycle during sporulation.

Catalytic activity

L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.

Pathway

Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.

Subunit structure

Homohexamer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the AlaDH/PNT family.

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Ontologies

Keywords
   Biological processSporulation
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

sporulation resulting in formation of a cellular spore

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionalanine dehydrogenase activity

Inferred from electronic annotation. Source: EC

binding

Inferred from electronic annotation. Source: InterPro

electron carrier activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 378378Alanine dehydrogenase
PRO_0000198992

Regions

Nucleotide binding168 – 19831NAD By similarity

Sites

Active site951 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q08352-1 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: B5D6113047FCF211

FASTA37839,684
        10         20         30         40         50         60 
MIIGVPKEIK NNENRVALTP GGVSQLISNG HRVLVETGAG LGSGFENEAY ESAGAEIIAD 

        70         80         90        100        110        120 
PKQVWDAEMV MKVKEPLPEE YVYFRKGLVL FTYLHLAAEP ELAQALKDKG VTAIAYETVS 

       130        140        150        160        170        180 
EGRTLPLLTP MSEVAGRMAA QIGAQFLEKP KGGKGILLAG VPGVSRGKVT IIGGGVVGTN 

       190        200        210        220        230        240 
AAKMAVGLGA DVTIIDLNAD RLRQLDDIFG HQIKTLISNP VNIADAVAEA DLLICAVLIP 

       250        260        270        280        290        300 
GAKAPTLVTE EMVKQMKPGS VIVDVAIDQG GIVETVDHIT THDQPTYEKH GVVHYAVANM 

       310        320        330        340        350        360 
PGAVPRTSTI ALTNVTVPYA LQIANKGAVK ALADNTALRA GLNTANGHVT YEAVARDLGY 

       370 
EYVPAEKALQ DESSVAGA

« Hide

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References

« Hide 'large scale' references
[1]"Alanine dehydrogenase (ald) is required for normal sporulation in Bacillus subtilis."
Siranosian K.J., Ireton K., Grossman A.D.
J. Bacteriol. 175:6789-6796(1993) [PubMed: 8226620] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"Sequence of a DNA fragment of 12315 bp around ald at about 253 degrees of the genome of Bacillus subtilis."
Oudega B., Vandenbol M., Koningstein G.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

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Cross-references

Sequence databases

L20916 Genomic DNA. Translation: AAA16038.1.
Z82015 Genomic DNA. Translation: CAB04775.1.
AL009126 Genomic DNA. Translation: CAB15181.1.
PIRA49337.
RefSeqNP_391071.1.

3D structure databases

HSSPHSSP built from PDB template 1SAY based on UniProtKB O52942.
ModBaseSearch...

Genome annotation databases

GeneID936557.
GenomeReviewsGene locus BSU31930 in contig AL009126_GR.
KEGGbsu:BSU31930.
NMPDRfig|224308.1.peg.3196.

Organism-specific databases

SubtiListBG10468. ald. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMQ08352.
OMAMGARVTI.

Enzyme and pathway databases

BioCycBSUB224308:BSU3188-MON.
MetaCyc:MON-12812.
BRENDA1.4.1.1. 150.

Family and domain databases

InterProIPR007698. Ala_DH/PNT_C.
IPR008142. Ala_DH/PNT_CS1.
IPR008143. Ala_DH/PNT_CS2.
IPR007886. Ala_DH/PNT_N.
IPR008141. Ala_DH/Pyr_transHydgase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
[Graphical view]
ProDomPD000139. FAD_pyr_redox. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00518. alaDH. 1 hit.
PROSITEPS00836. ALADH_PNT_1. 1 hit.
PS00837. ALADH_PNT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDHA_BACSU
AccessionPrimary (citable) accession number: Q08352
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 3, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents