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Q08351 (TPOR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thrombopoietin receptor

Short name=TPO-R
Alternative name(s):
Myeloproliferative leukemia protein
Proto-oncogene c-Mpl
CD_antigen=CD110
Gene names
Name:Mpl
Synonyms:Tpor
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length625 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses.

Subunit structure

Interacts with ATXN2L By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Induction

Expression in the bone marrow displays diurnal rhythmicity (a circadian rhythm that is synchronized with the day/night cycle). Ref.6

Domain

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Post-translational modification

Ubiquitination at Lys-544 and Lys-564 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination By similarity.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 1 subfamily.

Contains 2 fibronectin type-III domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 625600Thrombopoietin receptor
PRO_0000010988

Regions

Topological domain26 – 482457Extracellular Potential
Transmembrane483 – 50422Helical; Potential
Topological domain505 – 625121Cytoplasmic Potential
Domain178 – 27093Fibronectin type-III 1
Domain383 – 47997Fibronectin type-III 2
Motif465 – 4695WSXWS motif
Motif519 – 5279Box 1 motif
Compositional bias327 – 3304Poly-Glu

Amino acid modifications

Glycosylation1171N-linked (GlcNAc...) Potential
Cross-link544Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link564Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Experimental info

Sequence conflict1 – 77Missing in CAA52031. Ref.2
Sequence conflict991D → V in CAA52031. Ref.2
Sequence conflict2221P → PVRTSPAGE in CAA52031. Ref.2
Sequence conflict2411G → S in CAA80365. Ref.1
Sequence conflict2411G → S in CAA52031. Ref.2
Sequence conflict2411G → S in AAI46614. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q08351 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 87836E6EA4688ED7

FASTA62569,788
        10         20         30         40         50         60 
MPSWALFMVT SCLLLALPNQ AQVTSQDVFL LALGTEPLNC FSQTFEDLTC FWDEEEAAPS 

        70         80         90        100        110        120 
GTYQLLYAYR GEKPRACPLY SQSVPTFGTR YVCQFPAQDE VRLFFPLHLW VKNVSLNQTL 

       130        140        150        160        170        180 
IQRVLFVDSV GLPAPPRVIK ARGGSQPGEL QIHWEAPAPE ISDFLRHELR YGPTDSSNAT 

       190        200        210        220        230        240 
APSVIQLLST ETCCPTLWMP NPVPVLDQPP CVHPTASQPH GPAPFLTVKG GSCLVSGLQA 

       250        260        270        280        290        300 
GKSYWLQLRS QPDGVSLRGS WGPWSFPVTV DLPGDAVTIG LQCFTLDLKM VTCQWQQQDR 

       310        320        330        340        350        360 
TSSQGFFRHS RTRCCPTDRD PTWEKCEEEE PRPGSQPALV SRCHFKSRND SVIHILVEVT 

       370        380        390        400        410        420 
TAQGAVHSYL GSPFWIHQAV LLPTPSLHWR EVSSGRLELE WQHQSSWAAQ ETCYQLRYTG 

       430        440        450        460        470        480 
EGREDWKVLE PSLGARGGTL ELRPRARYSL QLRARLNGPT YQGPWSAWSP PARVSTGSET 

       490        500        510        520        530        540 
AWITLVTALL LVLSLSALLG LLLLKWQFPA HYRRLRHALW PSLPDLHRVL GQYLRDTAAL 

       550        560        570        580        590        600 
SPSKATVTDS CEEVEPSLLE ILPKSSESTP LPLCPSQPQM DYRGLQPCLR TMPLSVCPPM 

       610        620 
AETGSCCTTH IANHSYLPLS YWQQP 

« Hide

References

« Hide 'large scale' references
[1]"Murine c-mpl: a member of the hematopoietic growth factor receptor superfamily that transduces a proliferative signal."
Skoda R.C., Seldin D.C., Chiang M.K., Peichel C.L., Vogt T.F., Leder P.
EMBO J. 12:2645-2653(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"Characterization of the murine Mpl proto-oncogene, a member of the hematopoietic cytokine receptor family: molecular cloning, chromosomal location and evidence for a function in cell growth."
Vigon I., Florindo C., Fichelson S., Guenet J.-L., Mattei M.-G., Souyri M., Cosman D., Gisselbrecht S.
Oncogene 8:2607-2615(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICFW.
Tissue: Fetal liver.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Aorta and Vein.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Diurnal expression of the thrombopoietin gene is regulated by CLOCK."
Tracey C.J., Pan X., Catterson J.H., Harmar A.J., Hussain M.M., Hartley P.S.
J. Thromb. Haemost. 10:662-669(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z22649 mRNA. Translation: CAA80365.1.
Z22657 Genomic DNA. Translation: CAA80372.1.
X73677 mRNA. Translation: CAA52031.1.
AK041166 mRNA. Translation: BAC30846.1.
AL627212 Genomic DNA. Translation: CAM15212.1.
BC146613 mRNA. Translation: AAI46614.1.
CCDSCCDS18552.1.
PIRS35317.
S37622.
RefSeqNP_001116421.1. NM_001122949.2.
NP_001272425.1. NM_001285496.1.
NP_001272426.1. NM_001285497.1.
NP_034953.2. NM_010823.3.
UniGeneMm.4864.

3D structure databases

ProteinModelPortalQ08351.
SMRQ08351. Positions 25-270, 391-479.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201478. 3 interactions.
IntActQ08351. 1 interaction.

Chemistry

ChEMBLCHEMBL1075309.

PTM databases

PhosphoSiteQ08351.

Proteomic databases

PRIDEQ08351.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102671; ENSMUSP00000099732; ENSMUSG00000006389.
GeneID17480.
KEGGmmu:17480.
UCSCuc008uke.2. mouse.

Organism-specific databases

CTD4352.
MGIMGI:97076. Mpl.

Phylogenomic databases

eggNOGNOG39882.
GeneTreeENSGT00730000111277.
HOGENOMHOG000138191.
HOVERGENHBG000085.
KOK05082.
OrthoDBEOG77126V.
TreeFamTF336573.

Gene expression databases

ArrayExpressQ08351.
BgeeQ08351.
CleanExMM_MPL.
GenevestigatorQ08351.

Family and domain databases

Gene3D2.60.40.10. 4 hits.
InterProIPR003961. Fibronectin_type3.
IPR015152. Growth/epo_recpt_lig-bind.
IPR013783. Ig-like_fold.
IPR003528. Long_hematopoietin_rcpt_CS.
[Graphical view]
PfamPF09067. EpoR_lig-bind. 1 hit.
[Graphical view]
SMARTSM00060. FN3. 2 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 4 hits.
PROSITEPS50853. FN3. 1 hit.
PS01352. HEMATOPO_REC_L_F1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio292128.
PROQ08351.
SOURCESearch...

Entry information

Entry nameTPOR_MOUSE
AccessionPrimary (citable) accession number: Q08351
Secondary accession number(s): A6H8H6, Q8BRX0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot