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Protein

Thrombopoietin receptor

Gene

Mpl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses.

GO - Molecular functioni

GO - Biological processi

  • definitive hemopoiesis Source: MGI
  • homeostasis of number of cells Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • platelet formation Source: MGI
  • positive regulation of gene expression Source: MGI
  • regulation of chemokine production Source: MGI
  • regulation of stem cell division Source: MGI
  • regulation of stem cell proliferation Source: MGI

Keywordsi

Molecular functionReceptor

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombopoietin receptor
Short name:
TPO-R
Alternative name(s):
Myeloproliferative leukemia protein
Proto-oncogene c-Mpl
CD_antigen: CD110
Gene namesi
Name:Mpl
Synonyms:Tpor
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:97076. Mpl.

Subcellular locationi

  • Cell membrane; Single-pass type I membrane protein By similarity
  • Golgi apparatus By similarity
  • Cell surface By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 482ExtracellularSequence analysisAdd BLAST457
Transmembranei483 – 504HelicalSequence analysisAdd BLAST22
Topological domaini505 – 625CytoplasmicSequence analysisAdd BLAST121

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075309.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000001098826 – 625Thrombopoietin receptorAdd BLAST600

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi117N-linked (GlcNAc...) asparagineSequence analysis1
Cross-linki544Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki564Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitination at Lys-544 and Lys-564 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ08351.
PaxDbiQ08351.
PRIDEiQ08351.

PTM databases

iPTMnetiQ08351.
PhosphoSitePlusiQ08351.

Expressioni

Inductioni

Expression in the bone marrow displays diurnal rhythmicity (a circadian rhythm that is synchronized with the day/night cycle).1 Publication

Gene expression databases

BgeeiENSMUSG00000006389.
CleanExiMM_MPL.
ExpressionAtlasiQ08351. baseline and differential.
GenevisibleiQ08351. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with ATXN2L.By similarity

Protein-protein interaction databases

BioGridi201478. 3 interactors.
IntActiQ08351. 1 interactor.
STRINGi10090.ENSMUSP00000130167.

Structurei

3D structure databases

ProteinModelPortaliQ08351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 270Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST93
Domaini383 – 479Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST97

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi465 – 469WSXWS motif5
Motifi519 – 527Box 1 motif9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi327 – 330Poly-Glu4

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFK2. Eukaryota.
ENOG410YM4I. LUCA.
GeneTreeiENSGT00890000139533.
HOGENOMiHOG000138191.
HOVERGENiHBG000085.
InParanoidiQ08351.
KOiK05082.
TreeFamiTF336573.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 4 hits.
InterProiView protein in InterPro
IPR003961. FN3_dom.
IPR015152. Growth/epo_recpt_lig-bind.
IPR013783. Ig-like_fold.
IPR003528. Long_hematopoietin_rcpt_CS.
PfamiView protein in Pfam
PF09067. EpoR_lig-bind. 1 hit.
SMARTiView protein in SMART
SM00060. FN3. 2 hits.
SUPFAMiSSF49265. SSF49265. 4 hits.
PROSITEiView protein in PROSITE
PS50853. FN3. 1 hit.
PS01352. HEMATOPO_REC_L_F1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08351-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSWALFMVT SCLLLALPNQ AQVTSQDVFL LALGTEPLNC FSQTFEDLTC
60 70 80 90 100
FWDEEEAAPS GTYQLLYAYR GEKPRACPLY SQSVPTFGTR YVCQFPAQDE
110 120 130 140 150
VRLFFPLHLW VKNVSLNQTL IQRVLFVDSV GLPAPPRVIK ARGGSQPGEL
160 170 180 190 200
QIHWEAPAPE ISDFLRHELR YGPTDSSNAT APSVIQLLST ETCCPTLWMP
210 220 230 240 250
NPVPVLDQPP CVHPTASQPH GPAPFLTVKG GSCLVSGLQA GKSYWLQLRS
260 270 280 290 300
QPDGVSLRGS WGPWSFPVTV DLPGDAVTIG LQCFTLDLKM VTCQWQQQDR
310 320 330 340 350
TSSQGFFRHS RTRCCPTDRD PTWEKCEEEE PRPGSQPALV SRCHFKSRND
360 370 380 390 400
SVIHILVEVT TAQGAVHSYL GSPFWIHQAV LLPTPSLHWR EVSSGRLELE
410 420 430 440 450
WQHQSSWAAQ ETCYQLRYTG EGREDWKVLE PSLGARGGTL ELRPRARYSL
460 470 480 490 500
QLRARLNGPT YQGPWSAWSP PARVSTGSET AWITLVTALL LVLSLSALLG
510 520 530 540 550
LLLLKWQFPA HYRRLRHALW PSLPDLHRVL GQYLRDTAAL SPSKATVTDS
560 570 580 590 600
CEEVEPSLLE ILPKSSESTP LPLCPSQPQM DYRGLQPCLR TMPLSVCPPM
610 620
AETGSCCTTH IANHSYLPLS YWQQP
Length:625
Mass (Da):69,788
Last modified:July 27, 2011 - v2
Checksum:i87836E6EA4688ED7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 7Missing in CAA52031 (PubMed:8397366).Curated7
Sequence conflicti99D → V in CAA52031 (PubMed:8397366).Curated1
Sequence conflicti222P → PVRTSPAGE in CAA52031 (PubMed:8397366).Curated1
Sequence conflicti241G → S in CAA80365 (PubMed:8334987).Curated1
Sequence conflicti241G → S in CAA52031 (PubMed:8397366).Curated1
Sequence conflicti241G → S in AAI46614 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22649 mRNA. Translation: CAA80365.1.
Z22657 Genomic DNA. Translation: CAA80372.1.
X73677 mRNA. Translation: CAA52031.1.
AK041166 mRNA. Translation: BAC30846.1.
AL627212 Genomic DNA. Translation: CAM15212.1.
BC146613 mRNA. Translation: AAI46614.1.
CCDSiCCDS18552.1.
PIRiS35317.
S37622.
RefSeqiNP_001116421.1. NM_001122949.2.
NP_001272425.1. NM_001285496.1.
NP_001272426.1. NM_001285497.1.
NP_034953.2. NM_010823.3.
UniGeneiMm.4864.

Genome annotation databases

EnsembliENSMUST00000102671; ENSMUSP00000099732; ENSMUSG00000006389.
GeneIDi17480.
KEGGimmu:17480.
UCSCiuc008uke.3. mouse.

Similar proteinsi

Entry informationi

Entry nameiTPOR_MOUSE
AccessioniPrimary (citable) accession number: Q08351
Secondary accession number(s): A6H8H6, Q8BRX0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: August 30, 2017
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families