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Q08351

- TPOR_MOUSE

UniProt

Q08351 - TPOR_MOUSE

Protein

Thrombopoietin receptor

Gene

Mpl

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses.

    GO - Molecular functioni

    1. cytokine receptor activity Source: InterPro

    GO - Biological processi

    1. homeostasis of number of cells Source: MGI
    2. regulation of chemokine production Source: MGI

    Keywords - Molecular functioni

    Receptor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thrombopoietin receptor
    Short name:
    TPO-R
    Alternative name(s):
    Myeloproliferative leukemia protein
    Proto-oncogene c-Mpl
    CD_antigen: CD110
    Gene namesi
    Name:Mpl
    Synonyms:Tpor
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:97076. Mpl.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 625600Thrombopoietin receptorPRO_0000010988Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence Analysis
    Cross-linki544 – 544Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki564 – 564Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Post-translational modificationi

    Ubiquitination at Lys-544 and Lys-564 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination By similarity.By similarity

    Keywords - PTMi

    Glycoprotein, Isopeptide bond, Ubl conjugation

    Proteomic databases

    PRIDEiQ08351.

    PTM databases

    PhosphoSiteiQ08351.

    Expressioni

    Inductioni

    Expression in the bone marrow displays diurnal rhythmicity (a circadian rhythm that is synchronized with the day/night cycle).1 Publication

    Gene expression databases

    ArrayExpressiQ08351.
    BgeeiQ08351.
    CleanExiMM_MPL.
    GenevestigatoriQ08351.

    Interactioni

    Subunit structurei

    Interacts with ATXN2L.By similarity

    Protein-protein interaction databases

    BioGridi201478. 3 interactions.
    IntActiQ08351. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08351.
    SMRiQ08351. Positions 25-270, 391-479.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini26 – 482457ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini505 – 625121CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei483 – 50422HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini178 – 27093Fibronectin type-III 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini383 – 47997Fibronectin type-III 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi465 – 4695WSXWS motif
    Motifi519 – 5279Box 1 motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi327 – 3304Poly-Glu

    Domaini

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

    Sequence similaritiesi

    Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG39882.
    GeneTreeiENSGT00730000111277.
    HOGENOMiHOG000138191.
    HOVERGENiHBG000085.
    KOiK05082.
    OrthoDBiEOG77126V.
    TreeFamiTF336573.

    Family and domain databases

    Gene3Di2.60.40.10. 4 hits.
    InterProiIPR003961. Fibronectin_type3.
    IPR015152. Growth/epo_recpt_lig-bind.
    IPR013783. Ig-like_fold.
    IPR003528. Long_hematopoietin_rcpt_CS.
    [Graphical view]
    PfamiPF09067. EpoR_lig-bind. 1 hit.
    [Graphical view]
    SMARTiSM00060. FN3. 2 hits.
    [Graphical view]
    SUPFAMiSSF49265. SSF49265. 4 hits.
    PROSITEiPS50853. FN3. 1 hit.
    PS01352. HEMATOPO_REC_L_F1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q08351-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSWALFMVT SCLLLALPNQ AQVTSQDVFL LALGTEPLNC FSQTFEDLTC    50
    FWDEEEAAPS GTYQLLYAYR GEKPRACPLY SQSVPTFGTR YVCQFPAQDE 100
    VRLFFPLHLW VKNVSLNQTL IQRVLFVDSV GLPAPPRVIK ARGGSQPGEL 150
    QIHWEAPAPE ISDFLRHELR YGPTDSSNAT APSVIQLLST ETCCPTLWMP 200
    NPVPVLDQPP CVHPTASQPH GPAPFLTVKG GSCLVSGLQA GKSYWLQLRS 250
    QPDGVSLRGS WGPWSFPVTV DLPGDAVTIG LQCFTLDLKM VTCQWQQQDR 300
    TSSQGFFRHS RTRCCPTDRD PTWEKCEEEE PRPGSQPALV SRCHFKSRND 350
    SVIHILVEVT TAQGAVHSYL GSPFWIHQAV LLPTPSLHWR EVSSGRLELE 400
    WQHQSSWAAQ ETCYQLRYTG EGREDWKVLE PSLGARGGTL ELRPRARYSL 450
    QLRARLNGPT YQGPWSAWSP PARVSTGSET AWITLVTALL LVLSLSALLG 500
    LLLLKWQFPA HYRRLRHALW PSLPDLHRVL GQYLRDTAAL SPSKATVTDS 550
    CEEVEPSLLE ILPKSSESTP LPLCPSQPQM DYRGLQPCLR TMPLSVCPPM 600
    AETGSCCTTH IANHSYLPLS YWQQP 625
    Length:625
    Mass (Da):69,788
    Last modified:July 27, 2011 - v2
    Checksum:i87836E6EA4688ED7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 77Missing in CAA52031. (PubMed:8397366)Curated
    Sequence conflicti99 – 991D → V in CAA52031. (PubMed:8397366)Curated
    Sequence conflicti222 – 2221P → PVRTSPAGE in CAA52031. (PubMed:8397366)Curated
    Sequence conflicti241 – 2411G → S in CAA80365. (PubMed:8334987)Curated
    Sequence conflicti241 – 2411G → S in CAA52031. (PubMed:8397366)Curated
    Sequence conflicti241 – 2411G → S in AAI46614. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22649 mRNA. Translation: CAA80365.1.
    Z22657 Genomic DNA. Translation: CAA80372.1.
    X73677 mRNA. Translation: CAA52031.1.
    AK041166 mRNA. Translation: BAC30846.1.
    AL627212 Genomic DNA. Translation: CAM15212.1.
    BC146613 mRNA. Translation: AAI46614.1.
    CCDSiCCDS18552.1.
    PIRiS35317.
    S37622.
    RefSeqiNP_001116421.1. NM_001122949.2.
    NP_001272425.1. NM_001285496.1.
    NP_001272426.1. NM_001285497.1.
    NP_034953.2. NM_010823.3.
    UniGeneiMm.4864.

    Genome annotation databases

    EnsembliENSMUST00000102671; ENSMUSP00000099732; ENSMUSG00000006389.
    GeneIDi17480.
    KEGGimmu:17480.
    UCSCiuc008uke.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22649 mRNA. Translation: CAA80365.1 .
    Z22657 Genomic DNA. Translation: CAA80372.1 .
    X73677 mRNA. Translation: CAA52031.1 .
    AK041166 mRNA. Translation: BAC30846.1 .
    AL627212 Genomic DNA. Translation: CAM15212.1 .
    BC146613 mRNA. Translation: AAI46614.1 .
    CCDSi CCDS18552.1.
    PIRi S35317.
    S37622.
    RefSeqi NP_001116421.1. NM_001122949.2.
    NP_001272425.1. NM_001285496.1.
    NP_001272426.1. NM_001285497.1.
    NP_034953.2. NM_010823.3.
    UniGenei Mm.4864.

    3D structure databases

    ProteinModelPortali Q08351.
    SMRi Q08351. Positions 25-270, 391-479.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201478. 3 interactions.
    IntActi Q08351. 1 interaction.

    Chemistry

    ChEMBLi CHEMBL1075309.

    PTM databases

    PhosphoSitei Q08351.

    Proteomic databases

    PRIDEi Q08351.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102671 ; ENSMUSP00000099732 ; ENSMUSG00000006389 .
    GeneIDi 17480.
    KEGGi mmu:17480.
    UCSCi uc008uke.2. mouse.

    Organism-specific databases

    CTDi 4352.
    MGIi MGI:97076. Mpl.

    Phylogenomic databases

    eggNOGi NOG39882.
    GeneTreei ENSGT00730000111277.
    HOGENOMi HOG000138191.
    HOVERGENi HBG000085.
    KOi K05082.
    OrthoDBi EOG77126V.
    TreeFami TF336573.

    Miscellaneous databases

    NextBioi 292128.
    PROi Q08351.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q08351.
    Bgeei Q08351.
    CleanExi MM_MPL.
    Genevestigatori Q08351.

    Family and domain databases

    Gene3Di 2.60.40.10. 4 hits.
    InterProi IPR003961. Fibronectin_type3.
    IPR015152. Growth/epo_recpt_lig-bind.
    IPR013783. Ig-like_fold.
    IPR003528. Long_hematopoietin_rcpt_CS.
    [Graphical view ]
    Pfami PF09067. EpoR_lig-bind. 1 hit.
    [Graphical view ]
    SMARTi SM00060. FN3. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49265. SSF49265. 4 hits.
    PROSITEi PS50853. FN3. 1 hit.
    PS01352. HEMATOPO_REC_L_F1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Murine c-mpl: a member of the hematopoietic growth factor receptor superfamily that transduces a proliferative signal."
      Skoda R.C., Seldin D.C., Chiang M.K., Peichel C.L., Vogt T.F., Leder P.
      EMBO J. 12:2645-2653(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    2. "Characterization of the murine Mpl proto-oncogene, a member of the hematopoietic cytokine receptor family: molecular cloning, chromosomal location and evidence for a function in cell growth."
      Vigon I., Florindo C., Fichelson S., Guenet J.-L., Mattei M.-G., Souyri M., Cosman D., Gisselbrecht S.
      Oncogene 8:2607-2615(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICFW.
      Tissue: Fetal liver.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Aorta and Vein.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Diurnal expression of the thrombopoietin gene is regulated by CLOCK."
      Tracey C.J., Pan X., Catterson J.H., Harmar A.J., Hussain M.M., Hartley P.S.
      J. Thromb. Haemost. 10:662-669(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiTPOR_MOUSE
    AccessioniPrimary (citable) accession number: Q08351
    Secondary accession number(s): A6H8H6, Q8BRX0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3