Q08334 (I10R2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-10 receptor subunit beta
Short name=IL-10 receptor subunit beta
Short name=IL-10R subunit beta
Short name=IL-10RB

Alternative name(s):
Cytokine receptor class-II member 4
Cytokine receptor family 2 member 4
Short name=CRF2-4
Interleukin-10 receptor subunit 2
Short name=IL-10R subunit 2
Short name=IL-10R2
CD_antigen=CDw210b

Gene names

Name:	IL10RB
Synonyms:	CRFB4, D21S58, D21S66

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IFNL1. Ref.9
Subcellular location	Membrane; Single-pass type I membrane protein.
Polymorphism	Genetic variations in IL10RB influence susceptibility to hepatitis B virus (HBV) infection [MIM:610424].
Involvement in disease	Inflammatory bowel disease 25 (IBD25) [MIM:612567]: A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.11
Sequence similarities	Belongs to the type II cytokine receptor family. Contains 1 fibronectin type-III domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	immune response Traceable author statement PubMed 9463407. Source: ProtInc inflammatory response Traceable author statement PubMed 10954742. Source: ProtInc signal transduction Traceable author statement PubMed 10954742. Source: ProtInc
Cellular_component	interleukin-28 receptor complex Non-traceable author statement PubMed 12483210. Source: UniProtKB
Molecular_function	receptor activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Ref.6

Chain

20 – 325

306

Interleukin-10 receptor subunit beta

PRO_0000011014

Regions

Topological domain

20 – 220

201

Extracellular Potential

Transmembrane

221 – 242

22

Helical; Potential

Topological domain

243 – 325

83

Cytoplasmic Potential

Domain

113 – 205

93

Fibronectin type-III

Amino acid modifications

Glycosylation

49

1

N-linked (GlcNAc...) Potential

Glycosylation

68

1

N-linked (GlcNAc...) Potential

Glycosylation

102

1

N-linked (GlcNAc...) Potential

Glycosylation

161

1

N-linked (GlcNAc...) Potential

Disulfide bond

66 ↔ 74

Ref.12

Disulfide bond

188 ↔ 209

Ref.12

Natural variations

Natural variant

47

1

K → E Associated with susceptibility to HBV infection; higher cell surface levels. Ref.4 Ref.10
Corresponds to variant rs2834167 [ dbSNP | Ensembl ].

VAR_020666

Experimental info

Sequence conflict

124

1

A → D in AAA86872. Ref.2

Sequence conflict

243

1

A → S in CAA78933. Ref.1

Sequence conflict

243

1

A → S in AAA86872. Ref.2

Sequence conflict

269 – 273

5

FLGHP → VGRME in AAA86872. Ref.2

Sequence conflict

274 – 325

52

Missing in AAA86872. Ref.2

Secondary structure

1

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325

Helix Strand Turn

Details...

Beta strand

25 – 32

8

Beta strand

35 – 41

7

Beta strand

46 – 48

3

Beta strand

51 – 58

8

Beta strand

61 – 75

15

Beta strand

81 – 83

3

Beta strand

85 – 93

9

Beta strand

101 – 105

5

Helix

107 – 110

4

Beta strand

117 – 123

7

Beta strand

126 – 132

7

Beta strand

135 – 137

3

Beta strand

142 – 144

3

Helix

145 – 148

4

Beta strand

153 – 160

8

Beta strand

167 – 169

3

Beta strand

172 – 178

7

Beta strand

186 – 194

9

Turn

196 – 198

3

Beta strand

208 – 211

4

Helix

215 – 217

3

Sequences

	Sequence		Length	Mass (Da)
	Q08334 [UniParc]. Last modified December 21, 2004. Version 2. Checksum: E470726619AF54C2	FASTA	325	36,995
	10 20 30 40 50 60 MAWSLGSWLG GCLLVSALGM VPPPENVRMN SVNFKNILQW ESPAFAKGNL TFTAQYLSYR 70 80 90 100 110 120 IFQDKCMNTT LTECDFSSLS KYGDHTLRVR AEFADEHSDW VNITFCPVDD TIIGPPGMQV 130 140 150 160 170 180 EVLADSLHMR FLAPKIENEY ETWTMKNVYN SWTYNVQYWK NGTDEKFQIT PQYDFEVLRN 190 200 210 220 230 240 LEPWTTYCVQ VRGFLPDRNK AGEWSEPVCE QTTHDETVPS WMVAVILMAS VFMVCLALLG 250 260 270 280 290 300 CFALLWCVYK KTKYAFSPRN SLPQHLKEFL GHPHHNTLLF FSFPLSDEND VFDKLSVIAE 310 320 DSESGKQNPG DSCSLGTPPG QGPQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new member of the cytokine receptor gene family maps on chromosome 21 at less than 35 kb from IFNAR." Lutfalla G., Gardiner K., Uze G. Genomics 16:366-373(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[2]	"Structure of the human CRFB4 gene: comparison with its IFNAR neighbor." Lutfalla G., McInnis M.G., Antonarakis S.E., Uze G. J. Mol. Evol. 41:338-344(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	SeattleSNPs variation discovery resource Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLU-47.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[6]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-34.
[7]	"Identification and functional characterization of a second chain of the interleukin-10 receptor complex." Kotenko S.V., Krause C.D., Izotova L.S., Pollack B.P., Wu W., Pestka S. EMBO J. 16:5894-5903(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[8]	"Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R." Xie M.-H., Aggarwal S., Ho W.-H., Foster J., Zhang Z., Stinson J., Wood W.I., Goddard A.D., Gurney A.L. J. Biol. Chem. 275:31335-31339(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[9]	"The expanded family of class II cytokines that share the IL-10 receptor-2 (IL-10R2) chain." Donnelly R.P., Sheikh F., Kotenko S.V., Dickensheets H. J. Leukoc. Biol. 76:314-321(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Class II cytokine receptor gene cluster is a major locus for hepatitis B persistence." Frodsham A.J., Zhang L., Dumpis U., Taib N.A.M., Best S., Durham A., Hennig B.J.W., Hellier S., Knapp S., Wright M., Chiaramonte M., Bell J.I., Graves M., Whittle H.C., Thomas H.C., Thursz M.R., Hill A.V.S. Proc. Natl. Acad. Sci. U.S.A. 103:9148-9153(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INVOLVEMENT IN SUSCEPTIBILITY TO HBV INFECTION, VARIANT GLU-47, CHARACTERIZATION OF VARIANT GLU-47.
[11]	"Inflammatory bowel disease and mutations affecting the interleukin-10 receptor." Glocker E.O., Kotlarz D., Boztug K., Gertz E.M., Schaffer A.A., Noyan F., Perro M., Diestelhorst J., Allroth A., Murugan D., Hatscher N., Pfeifer D., Sykora K.W., Sauer M., Kreipe H., Lacher M., Nustede R., Woellner C. , Baumann U., Salzer U., Koletzko S., Shah N., Segal A.W., Sauerbrey A., Buderus S., Snapper S.B., Grimbacher B., Klein C. N. Engl. J. Med. 361:2033-2045(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INVOLVEMENT IN IBD25.
[12]	"Structure and mechanism of receptor sharing by the IL-10R2 common chain." Yoon S.I., Jones B.C., Logsdon N.J., Harris B.D., Deshpande A., Radaeva S., Halloran B.A., Gao B., Walter M.R. Structure 18:638-648(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.14 ANGSTROMS) OF 20-220, DISULFIDE BONDS.
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z17227 mRNA. Translation: CAA78933.1.
U08988 Genomic DNA. Translation: AAA86872.1.
BT009777 mRNA. Translation: AAP88779.1.
AY323826 Genomic DNA. Translation: AAP72016.1.
BC001903 mRNA. Translation: AAH01903.1.

IPI

IPI00646217.

PIR

A47003.

RefSeq

NP_000619.3. NM_000628.4.

UniGene

Hs.654593.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3LQM	X-ray	2.14	A/B	20-220	[»]

ProteinModelPortal

Q08334.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6016N.

STRING

9606.ENSP00000290200.

PTM databases

PhosphoSite

Q08334.

Polymorphism databases

DMDM

56757647.

Proteomic databases

PaxDb

Q08334.

PRIDE

Q08334.

Protocols and materials databases

DNASU

3588.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000290200; ENSP00000290200; ENSG00000243646.

GeneID

3588.

KEGG

hsa:3588.

UCSC

uc002yrh.1. human.

Organism-specific databases

CTD

3588.

GeneCards

GC21P034638.

H-InvDB

HIX0213245.

HGNC

HGNC:5965. IL10RB.

HPA

CAB020670.

MIM

123889. gene.
610424. phenotype.
612567. phenotype.

neXtProt

NX_Q08334.

Orphanet

238569. Autosomal recessive early-onset inflammatory bowel disease.

PharmGKB

PA29780.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG47180.

HOVERGEN

HBG052057.

InParanoid

Q08334.

KO

K05135.

OMA

SDENDVF.

OrthoDB

EOG4H19W1.

PhylomeDB

Q08334.

Gene expression databases

ArrayExpress

Q08334.

Bgee

Q08334.

CleanEx

HS_IL10RB.

Genevestigator

Q08334.

GermOnline

ENSG00000159113. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.

InterPro

IPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
IPR015373. Interferon_alpha/beta_rcpt_bsu.
[Graphical view]

Pfam

PF09294. Interfer-bind. 1 hit.
[Graphical view]

SMART

SM00060. FN3. 2 hits.
[Graphical view]

SUPFAM

SSF49265. FN_III-like. 2 hits.

PROSITE

PS50853. FN3. False negative.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

IL10RB. human.

EvolutionaryTrace

Q08334.

GenomeRNAi

3588.

NextBio

14019.

SOURCE

Search...

Entry information

Entry name

I10R2_HUMAN

Accession

Primary (citable) accession number: Q08334
Secondary accession number(s): Q9BUU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.