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Protein

Guanine nucleotide exchange factor MSS4

Gene

Rabif

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Guanine-nucleotide-releasing protein that acts on members of the SEC4/YPT1/RAB subfamily. Stimulates GDP release from both YPT1 and RAB3A, but is less active on these proteins than on the SEC4 protein. Might play a general role in vesicular transport.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi23 – 231Zinc
Metal bindingi26 – 261Zinc
Metal bindingi94 – 941Zinc
Metal bindingi97 – 971Zinc

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor MSS4
Alternative name(s):
Rab-interacting factor
Gene namesi
Name:Rabif
Synonyms:Mss4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componentsi: Chromosome 13, Chromosome 2

Organism-specific databases

RGDi1359331. Rabif.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 123123Guanine nucleotide exchange factor MSS4PRO_0000174176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ08326.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

ExpressionAtlasiQ08326. baseline and differential.
GenevisibleiQ08326. RN.

Interactioni

Subunit structurei

Interacts with RAB8A.By similarity

Protein-protein interaction databases

BioGridi257986. 1 interaction.
STRINGi10116.ENSRNOP00000066145.

Structurei

Secondary structure

1
123
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi15 – 195Combined sources
Beta strandi21 – 233Combined sources
Turni24 – 263Combined sources
Beta strandi29 – 313Combined sources
Beta strandi35 – 395Combined sources
Beta strandi43 – 464Combined sources
Beta strandi62 – 654Combined sources
Beta strandi68 – 725Combined sources
Helixi74 – 763Combined sources
Beta strandi89 – 935Combined sources
Turni95 – 973Combined sources
Beta strandi102 – 1065Combined sources
Beta strandi113 – 1164Combined sources
Helixi117 – 1193Combined sources
Beta strandi120 – 1223Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HXRX-ray1.65A/B9-123[»]
ProteinModelPortaliQ08326.
SMRiQ08326. Positions 9-123.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08326.

Family & Domainsi

Sequence similaritiesi

Belongs to the DSS4/MSS4 family.Curated

Phylogenomic databases

eggNOGiKOG4113. Eukaryota.
ENOG4111UFA. LUCA.
GeneTreeiENSGT00390000016889.
HOGENOMiHOG000007903.
HOVERGENiHBG006402.
InParanoidiQ08326.
KOiK19952.
OMAiGWHSLDD.
OrthoDBiEOG76DTVD.
PhylomeDBiQ08326.
TreeFamiTF314029.

Family and domain databases

Gene3Di2.170.150.10. 1 hit.
InterProiIPR007515. Mss4.
IPR011057. Mss4-like.
IPR011323. Mss4/transl-control_tumor.
[Graphical view]
PfamiPF04421. Mss4. 1 hit.
[Graphical view]
SUPFAMiSSF51316. SSF51316. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPCELQNEL VSAEGRNRKA VLCQRCGSRV LQPGTALFSR RQLFLPSMRK
60 70 80 90 100
KPDLVDGSNP DGDVLEEHWL VNDMFIFENV GFTKDVGNVK FLVCADCEIG
110 120
PIGWHCLDDK NSFYVALERV SHE
Length:123
Mass (Da):13,928
Last modified:February 1, 1995 - v1
Checksum:iACB7D07E2735811C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70496 mRNA. Translation: CAA49904.1.
PIRiS29714.
RefSeqiNP_001007679.1. NM_001007678.2.
XP_003749349.1. XM_003749301.3.
UniGeneiRn.204985.

Genome annotation databases

EnsembliENSRNOT00000005845; ENSRNOP00000005845; ENSRNOG00000004424.
ENSRNOT00000073716; ENSRNOP00000066145; ENSRNOG00000045814.
GeneIDi304807.
499636.
KEGGirno:304807.
rno:499636.
UCSCiRGD:1359331. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70496 mRNA. Translation: CAA49904.1.
PIRiS29714.
RefSeqiNP_001007679.1. NM_001007678.2.
XP_003749349.1. XM_003749301.3.
UniGeneiRn.204985.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HXRX-ray1.65A/B9-123[»]
ProteinModelPortaliQ08326.
SMRiQ08326. Positions 9-123.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi257986. 1 interaction.
STRINGi10116.ENSRNOP00000066145.

Proteomic databases

PaxDbiQ08326.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005845; ENSRNOP00000005845; ENSRNOG00000004424.
ENSRNOT00000073716; ENSRNOP00000066145; ENSRNOG00000045814.
GeneIDi304807.
499636.
KEGGirno:304807.
rno:499636.
UCSCiRGD:1359331. rat.

Organism-specific databases

CTDi499636.
5877.
RGDi1359331. Rabif.

Phylogenomic databases

eggNOGiKOG4113. Eukaryota.
ENOG4111UFA. LUCA.
GeneTreeiENSGT00390000016889.
HOGENOMiHOG000007903.
HOVERGENiHBG006402.
InParanoidiQ08326.
KOiK19952.
OMAiGWHSLDD.
OrthoDBiEOG76DTVD.
PhylomeDBiQ08326.
TreeFamiTF314029.

Miscellaneous databases

EvolutionaryTraceiQ08326.
NextBioi653652.
PROiQ08326.

Gene expression databases

ExpressionAtlasiQ08326. baseline and differential.
GenevisibleiQ08326. RN.

Family and domain databases

Gene3Di2.170.150.10. 1 hit.
InterProiIPR007515. Mss4.
IPR011057. Mss4-like.
IPR011323. Mss4/transl-control_tumor.
[Graphical view]
PfamiPF04421. Mss4. 1 hit.
[Graphical view]
SUPFAMiSSF51316. SSF51316. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A mammalian guanine-nucleotide-releasing protein enhances function of yeast secretory protein Sec4."
    Burton J., Roberts D., Montaldi M., Novick P., de Camilli P.
    Nature 361:464-467(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "A helical turn motif in Mss4 is a critical determinant of Rab binding and nucleotide release."
    Zhu Z., Dumas J.J., Lietzke S.E., Lambright D.G.
    Biochemistry 40:3027-3036(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 9-123, ZINC-BINDING SITES.

Entry informationi

Entry nameiMSS4_RAT
AccessioniPrimary (citable) accession number: Q08326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.