Q08307 (PPOE_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Polyphenol oxidase E, chloroplastic Short name=PPO EC=1.10.3.1 Alternative name(s): Catechol oxidase
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.
Catalytic activity	2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.
Cofactor	Binds 2 copper ions per subunit By similarity.
Subcellular location	Plastid › chloroplast thylakoid lumen.
Sequence similarities	Belongs to the tyrosinase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid Thylakoid
Domain	Transit peptide
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond Thioether bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pigment biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast thylakoid lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	catechol oxidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 87	87	Chloroplast
	Chain	88 – 587	500	Polyphenol oxidase E, chloroplastic		PRO_0000035914
Sites
	Metal binding	179	1	Copper A By similarity
	Metal binding	197	1	Copper A By similarity
	Metal binding	206	1	Copper A By similarity
	Metal binding	328	1	Copper B By similarity
	Metal binding	332	1	Copper B By similarity
	Metal binding	363	1	Copper B By similarity
Amino acid modifications
	Disulfide bond	98 ↔ 114		By similarity
	Disulfide bond	113 ↔ 180		By similarity
	Cross-link	183 ↔ 197		2'-(S-cysteinyl)-histidine (Cys-His) By similarity
Experimental info
	Sequence conflict	311 – 315	5	PLGSE → LWVLN in AAB22610. Ref.2
	Sequence conflict	410	1	C → V in AAB22610. Ref.2
	Sequence conflict	541	1	L → V in AAB22610. Ref.2
	Sequence conflict	567	1	V → I in AAB22610. Ref.2
	Sequence conflict	574	1	E → G in AAB22610. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q08307 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A796AA1DD6454E3D	FASTA	587	66,237
	10 20 30 40 50 60 MSSSSSITTT LPLCTNKSLS SSFTTTNSSL LSKPSQLFLH GRRNQSFKVS CNANNVDKNP 70 80 90 100 110 120 DAVDRRNVLL GLGGLYGAAN LAPLATAAPI PPPDLKSCGT AHVKEGVDVI YSCCPPVPDD 130 140 150 160 170 180 IDSVPYYKFP SMTKLRIRPP AHAADEEYVA KYQLATSRMR ELDKDPFDPL GFKQQANIHC 190 200 210 220 230 240 AYCNGAYKVG GKELQVHFSW LFFPFHRWYL YFYERILGSL INDPTFALPY WNWDHPKGMR 250 260 270 280 290 300 IPPMFDREGS SLYDEKRNQN HRNGTIIDLG HFGKEVDTPQ LQIMTNNLTL MYRQMVTNAP 310 320 330 340 350 360 CPSQFFGAAY PLGSEPSPGQ GTIENIPHTP VHIWTGDKPR QKNGEDMGNF YSAGLDPIFY 370 380 390 400 410 420 CHHANVDRMW NEWKLIGGKR RDLTDKDWLN SEFFFYDENR NPYRVKVRDC LDSKKMGFDY 430 440 450 460 470 480 APMPTPWRNF KPIRKSSSGK VNTASIAPVS KVFPLAKLDR AISFSITRPA SSRTTQEKNE 490 500 510 520 530 540 QEEILTFNKI SYDDRNYVRF DVFLNVDKTV NADELDKAEF AGSYTSLPHV HGSNTNHVTS 550 560 570 580 LTFKLAITEL LEDIGLEDED TIAVTLVPKA GGEEVSIESV EIKLEDC

References

[1]	"Organisation of the tomato polyphenol oxidase gene family." Newman S.M., Eannetta N.T., Yu H., Prince J.P., de Vicente M.C., Tanksley S.D., Steffens J.C. Plant Mol. Biol. 21:1035-1051(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. VFNT Cherry.
[2]	"The tomato 66.3-kD polyphenoloxidase gene: molecular identification and developmental expression." Shahar T., Hennig N., Gutfinger T., Hareven D., Lifschitz E. Plant Cell 4:135-147(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Tiny tim.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z12837 Genomic DNA. Translation: CAA78299.1. S40548 Genomic DNA. Translation: AAB22610.1.
PIR	S33543.
3D structure databases
ProteinModelPortal	Q08307.
SMR	Q08307. Positions 88-428.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16342.
Family and domain databases
Gene3D	1.10.1280.10. 1 hit.
InterPro	IPR016213. Polyphenol_oxidase. IPR022740. Polyphenol_oxidase_C. IPR022739. Polyphenol_oxidase_cen. IPR002227. Tyrosinase. IPR008922. Unchr_di-copper_centre. [Graphical view]
Pfam	PF12142. PPO1_DWL. 1 hit. PF12143. PPO1_KFDV. 1 hit. PF00264. Tyrosinase. 1 hit. [Graphical view]
PIRSF	PIRSF000290. PPO_plant. 1 hit.
PRINTS	PR00092. TYROSINASE.
SUPFAM	SSF48056. Di-copper_centre. 1 hit.
PROSITE	PS00497. TYROSINASE_1. 1 hit. PS00498. TYROSINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPOE_SOLLC

Accession

Primary (citable) accession number: Q08307
Secondary accession number(s): Q41175

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program