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Q08307

- PPOE_SOLLC

UniProt

Q08307 - PPOE_SOLLC

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Protein
Polyphenol oxidase E, chloroplastic
Gene
N/A
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.

Catalytic activityi

2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.

Cofactori

Binds 2 copper ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi179 – 1791Copper A By similarity
Metal bindingi197 – 1971Copper A By similarity
Metal bindingi206 – 2061Copper A By similarity
Metal bindingi328 – 3281Copper B By similarity
Metal bindingi332 – 3321Copper B By similarity
Metal bindingi363 – 3631Copper B By similarity

GO - Molecular functioni

  1. catechol oxidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. pigment biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16342.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphenol oxidase E, chloroplastic (EC:1.10.3.1)
Short name:
PPO
Alternative name(s):
Catechol oxidase
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. chloroplast thylakoid lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8787Chloroplast
Add
BLAST
Chaini88 – 587500Polyphenol oxidase E, chloroplastic
PRO_0000035914Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi98 ↔ 114 By similarity
Disulfide bondi113 ↔ 180 By similarity
Cross-linki183 ↔ 1972'-(S-cysteinyl)-histidine (Cys-His) By similarity

Keywords - PTMi

Disulfide bond, Thioether bond

Structurei

3D structure databases

ProteinModelPortaliQ08307.
SMRiQ08307. Positions 88-428.

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000290. PPO_plant. 1 hit.
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08307-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSSSITTT LPLCTNKSLS SSFTTTNSSL LSKPSQLFLH GRRNQSFKVS    50
CNANNVDKNP DAVDRRNVLL GLGGLYGAAN LAPLATAAPI PPPDLKSCGT 100
AHVKEGVDVI YSCCPPVPDD IDSVPYYKFP SMTKLRIRPP AHAADEEYVA 150
KYQLATSRMR ELDKDPFDPL GFKQQANIHC AYCNGAYKVG GKELQVHFSW 200
LFFPFHRWYL YFYERILGSL INDPTFALPY WNWDHPKGMR IPPMFDREGS 250
SLYDEKRNQN HRNGTIIDLG HFGKEVDTPQ LQIMTNNLTL MYRQMVTNAP 300
CPSQFFGAAY PLGSEPSPGQ GTIENIPHTP VHIWTGDKPR QKNGEDMGNF 350
YSAGLDPIFY CHHANVDRMW NEWKLIGGKR RDLTDKDWLN SEFFFYDENR 400
NPYRVKVRDC LDSKKMGFDY APMPTPWRNF KPIRKSSSGK VNTASIAPVS 450
KVFPLAKLDR AISFSITRPA SSRTTQEKNE QEEILTFNKI SYDDRNYVRF 500
DVFLNVDKTV NADELDKAEF AGSYTSLPHV HGSNTNHVTS LTFKLAITEL 550
LEDIGLEDED TIAVTLVPKA GGEEVSIESV EIKLEDC 587
Length:587
Mass (Da):66,237
Last modified:November 1, 1995 - v1
Checksum:iA796AA1DD6454E3D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti311 – 3155PLGSE → LWVLN in AAB22610. 1 Publication
Sequence conflicti410 – 4101C → V in AAB22610. 1 Publication
Sequence conflicti541 – 5411L → V in AAB22610. 1 Publication
Sequence conflicti567 – 5671V → I in AAB22610. 1 Publication
Sequence conflicti574 – 5741E → G in AAB22610. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z12837 Genomic DNA. Translation: CAA78299.1.
S40548 Genomic DNA. Translation: AAB22610.1.
PIRiS33543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z12837 Genomic DNA. Translation: CAA78299.1 .
S40548 Genomic DNA. Translation: AAB22610.1 .
PIRi S33543.

3D structure databases

ProteinModelPortali Q08307.
SMRi Q08307. Positions 88-428.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16342.

Family and domain databases

Gene3Di 1.10.1280.10. 1 hit.
InterProi IPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view ]
Pfami PF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000290. PPO_plant. 1 hit.
PRINTSi PR00092. TYROSINASE.
SUPFAMi SSF48056. SSF48056. 1 hit.
PROSITEi PS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. VFNT Cherry.
  2. "The tomato 66.3-kD polyphenoloxidase gene: molecular identification and developmental expression."
    Shahar T., Hennig N., Gutfinger T., Hareven D., Lifschitz E.
    Plant Cell 4:135-147(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Tiny tim.

Entry informationi

Entry nameiPPOE_SOLLC
AccessioniPrimary (citable) accession number: Q08307
Secondary accession number(s): Q41175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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