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Protein

Polyphenol oxidase E, chloroplastic

Gene
N/A
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.

Catalytic activityi

2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.

Cofactori

Cu2+By similarityNote: Binds 2 copper ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi179 – 1791Copper ABy similarity
Metal bindingi197 – 1971Copper ABy similarity
Metal bindingi206 – 2061Copper ABy similarity
Metal bindingi328 – 3281Copper BBy similarity
Metal bindingi332 – 3321Copper BBy similarity
Metal bindingi363 – 3631Copper BBy similarity

GO - Molecular functioni

  1. catechol oxidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. pigment biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Copper, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16342.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphenol oxidase E, chloroplastic (EC:1.10.3.1)
Short name:
PPO
Alternative name(s):
Catechol oxidase
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. chloroplast thylakoid lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8787ChloroplastAdd
BLAST
Chaini88 – 587500Polyphenol oxidase E, chloroplasticPRO_0000035914Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi98 ↔ 114By similarity
Disulfide bondi113 ↔ 180By similarity
Cross-linki183 ↔ 1972'-(S-cysteinyl)-histidine (Cys-His)By similarity

Keywords - PTMi

Disulfide bond, Thioether bond

Structurei

3D structure databases

ProteinModelPortaliQ08307.
SMRiQ08307. Positions 88-428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tyrosinase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000290. PPO_plant. 1 hit.
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08307-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSSSITTT LPLCTNKSLS SSFTTTNSSL LSKPSQLFLH GRRNQSFKVS
60 70 80 90 100
CNANNVDKNP DAVDRRNVLL GLGGLYGAAN LAPLATAAPI PPPDLKSCGT
110 120 130 140 150
AHVKEGVDVI YSCCPPVPDD IDSVPYYKFP SMTKLRIRPP AHAADEEYVA
160 170 180 190 200
KYQLATSRMR ELDKDPFDPL GFKQQANIHC AYCNGAYKVG GKELQVHFSW
210 220 230 240 250
LFFPFHRWYL YFYERILGSL INDPTFALPY WNWDHPKGMR IPPMFDREGS
260 270 280 290 300
SLYDEKRNQN HRNGTIIDLG HFGKEVDTPQ LQIMTNNLTL MYRQMVTNAP
310 320 330 340 350
CPSQFFGAAY PLGSEPSPGQ GTIENIPHTP VHIWTGDKPR QKNGEDMGNF
360 370 380 390 400
YSAGLDPIFY CHHANVDRMW NEWKLIGGKR RDLTDKDWLN SEFFFYDENR
410 420 430 440 450
NPYRVKVRDC LDSKKMGFDY APMPTPWRNF KPIRKSSSGK VNTASIAPVS
460 470 480 490 500
KVFPLAKLDR AISFSITRPA SSRTTQEKNE QEEILTFNKI SYDDRNYVRF
510 520 530 540 550
DVFLNVDKTV NADELDKAEF AGSYTSLPHV HGSNTNHVTS LTFKLAITEL
560 570 580
LEDIGLEDED TIAVTLVPKA GGEEVSIESV EIKLEDC
Length:587
Mass (Da):66,237
Last modified:November 1, 1995 - v1
Checksum:iA796AA1DD6454E3D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti311 – 3155PLGSE → LWVLN in AAB22610 (PubMed:1633491).Curated
Sequence conflicti410 – 4101C → V in AAB22610 (PubMed:1633491).Curated
Sequence conflicti541 – 5411L → V in AAB22610 (PubMed:1633491).Curated
Sequence conflicti567 – 5671V → I in AAB22610 (PubMed:1633491).Curated
Sequence conflicti574 – 5741E → G in AAB22610 (PubMed:1633491).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12837 Genomic DNA. Translation: CAA78299.1.
S40548 Genomic DNA. Translation: AAB22610.1.
PIRiS33543.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12837 Genomic DNA. Translation: CAA78299.1.
S40548 Genomic DNA. Translation: AAB22610.1.
PIRiS33543.

3D structure databases

ProteinModelPortaliQ08307.
SMRiQ08307. Positions 88-428.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16342.

Family and domain databases

Gene3Di1.10.1280.10. 1 hit.
InterProiIPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamiPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000290. PPO_plant. 1 hit.
PRINTSiPR00092. TYROSINASE.
SUPFAMiSSF48056. SSF48056. 1 hit.
PROSITEiPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. VFNT Cherry.
  2. "The tomato 66.3-kD polyphenoloxidase gene: molecular identification and developmental expression."
    Shahar T., Hennig N., Gutfinger T., Hareven D., Lifschitz E.
    Plant Cell 4:135-147(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Tiny tim.

Entry informationi

Entry nameiPPOE_SOLLC
AccessioniPrimary (citable) accession number: Q08307
Secondary accession number(s): Q41175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.