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Q08306 (PPOD_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyphenol oxidase D, chloroplastic

Short name=PPO
EC=1.10.3.1
Alternative name(s):
Catechol oxidase
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length591 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.

Catalytic activity

2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.

Cofactor

Binds 2 copper ions per subunit By similarity.

Subcellular location

Plastidchloroplast thylakoid lumen.

Sequence similarities

Belongs to the tyrosinase family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
Thylakoid
   DomainTransit peptide
   LigandCopper
Metal-binding
   Molecular functionOxidoreductase
   PTMDisulfide bond
Thioether bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpigment biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast thylakoid lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncatechol oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 8383Chloroplast By similarity
Chain84 – 591508Polyphenol oxidase D, chloroplastic
PRO_0000035913

Sites

Metal binding1761Copper A By similarity
Metal binding1941Copper A By similarity
Metal binding2031Copper A By similarity
Metal binding3241Copper B By similarity
Metal binding3281Copper B By similarity
Metal binding3661Copper B By similarity

Amino acid modifications

Disulfide bond94 ↔ 110 By similarity
Disulfide bond109 ↔ 177 By similarity
Cross-link180 ↔ 1942'-(S-cysteinyl)-histidine (Cys-His) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q08306 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 083C08429E80C85F

FASTA59166,529
        10         20         30         40         50         60 
MASLCSNSST TSLKTPFTSL GSTPKPCQLF LHGKRNKAFK VSCKVTNTNG NQDETNSVDR 

        70         80         90        100        110        120 
RNVLLGLGGL YGVANAIPLA ASAAPTPPPD LSSCSIARID ENQVVSYSCC APKPDDMEKV 

       130        140        150        160        170        180 
PYYKFPSMTK LRVRQPAHEA DEEYIAKYNV SVTKMRDLDK TQPLNPIGFK QQANIHCAYC 

       190        200        210        220        230        240 
NGAYRIGGKE LQVHNSWLFF PFHRWYLYFY ESNAGKLIDD PTFALPYWNW DHPKGMRFPA 

       250        260        270        280        290        300 
MYDREGTFLF DETRDQSHRN GTVIDLGFFG NEVETTQLQM MSNNLTLMYR QMVTNAPCPR 

       310        320        330        340        350        360 
MFFGDLMISG ITLNSPGTIE NIPHGPVHIW SGTVRGSTLP NGAISKRGEY GHFYSAGLDP 

       370        380        390        400        410        420 
VFFCHHSNVD RMWSEWKATG GKRTDITHKD WLNSEFFFYD ENENPYRVKV RDCLDTKKMG 

       430        440        450        460        470        480 
YDYKPMRTPW RNFKPLTKAS AGKVNTSSIP PVSQAFPLAK LDKAVSFSIN RPTSSRTPQE 

       490        500        510        520        530        540 
KNAQEEMLTF NSIRYDNRGY IRFDVFLNVD NNVNANELDK AEFAGSYTSL PHVHRAGETN 

       550        560        570        580        590 
HIATVDFQLA ITELLEDIGL EDEDTIAVTL VPKRGGEGIS IENATISLAD C 

« Hide

References

[1]"Organisation of the tomato polyphenol oxidase gene family."
Newman S.M., Eannetta N.T., Yu H., Prince J.P., de Vicente M.C., Tanksley S.D., Steffens J.C.
Plant Mol. Biol. 21:1035-1051(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. VFNT Cherry.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z12836 Genomic DNA. Translation: CAA78298.1.
PIRS33542.

3D structure databases

ProteinModelPortalQ08306.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1280.10. 1 hit.
InterProIPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFPIRSF000290. PPO_plant. 1 hit.
PRINTSPR00092. TYROSINASE.
SUPFAMSSF48056. SSF48056. 1 hit.
PROSITEPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPOD_SOLLC
AccessionPrimary (citable) accession number: Q08306
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families