Q08306 (PPOD_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Polyphenol oxidase D, chloroplastic Short name=PPO EC=1.10.3.1 Alternative name(s): Catechol oxidase
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.
Catalytic activity	2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.
Cofactor	Binds 2 copper ions per subunit By similarity.
Subcellular location	Plastid › chloroplast thylakoid lumen.
Sequence similarities	Belongs to the tyrosinase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid Thylakoid
Domain	Transit peptide
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond Thioether bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pigment biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast thylakoid lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	catechol oxidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 83	83	Chloroplast By similarity
	Chain	84 – 591	508	Polyphenol oxidase D, chloroplastic		PRO_0000035913
Sites
	Metal binding	176	1	Copper A By similarity
	Metal binding	194	1	Copper A By similarity
	Metal binding	203	1	Copper A By similarity
	Metal binding	324	1	Copper B By similarity
	Metal binding	328	1	Copper B By similarity
	Metal binding	366	1	Copper B By similarity
Amino acid modifications
	Disulfide bond	94 ↔ 110		By similarity
	Disulfide bond	109 ↔ 177		By similarity
	Cross-link	180 ↔ 194		2'-(S-cysteinyl)-histidine (Cys-His) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08306 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 083C08429E80C85F	FASTA	591	66,529
	10 20 30 40 50 60 MASLCSNSST TSLKTPFTSL GSTPKPCQLF LHGKRNKAFK VSCKVTNTNG NQDETNSVDR 70 80 90 100 110 120 RNVLLGLGGL YGVANAIPLA ASAAPTPPPD LSSCSIARID ENQVVSYSCC APKPDDMEKV 130 140 150 160 170 180 PYYKFPSMTK LRVRQPAHEA DEEYIAKYNV SVTKMRDLDK TQPLNPIGFK QQANIHCAYC 190 200 210 220 230 240 NGAYRIGGKE LQVHNSWLFF PFHRWYLYFY ESNAGKLIDD PTFALPYWNW DHPKGMRFPA 250 260 270 280 290 300 MYDREGTFLF DETRDQSHRN GTVIDLGFFG NEVETTQLQM MSNNLTLMYR QMVTNAPCPR 310 320 330 340 350 360 MFFGDLMISG ITLNSPGTIE NIPHGPVHIW SGTVRGSTLP NGAISKRGEY GHFYSAGLDP 370 380 390 400 410 420 VFFCHHSNVD RMWSEWKATG GKRTDITHKD WLNSEFFFYD ENENPYRVKV RDCLDTKKMG 430 440 450 460 470 480 YDYKPMRTPW RNFKPLTKAS AGKVNTSSIP PVSQAFPLAK LDKAVSFSIN RPTSSRTPQE 490 500 510 520 530 540 KNAQEEMLTF NSIRYDNRGY IRFDVFLNVD NNVNANELDK AEFAGSYTSL PHVHRAGETN 550 560 570 580 590 HIATVDFQLA ITELLEDIGL EDEDTIAVTL VPKRGGEGIS IENATISLAD C

References

[1]	"Organisation of the tomato polyphenol oxidase gene family." Newman S.M., Eannetta N.T., Yu H., Prince J.P., de Vicente M.C., Tanksley S.D., Steffens J.C. Plant Mol. Biol. 21:1035-1051(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. VFNT Cherry.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z12836 Genomic DNA. Translation: CAA78298.1.
PIR	S33542.
3D structure databases
ProteinModelPortal	Q08306.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.1280.10. 1 hit.
InterPro	IPR016213. Polyphenol_oxidase. IPR022740. Polyphenol_oxidase_C. IPR022739. Polyphenol_oxidase_cen. IPR002227. Tyrosinase. IPR008922. Unchr_di-copper_centre. [Graphical view]
Pfam	PF12142. PPO1_DWL. 1 hit. PF12143. PPO1_KFDV. 1 hit. PF00264. Tyrosinase. 1 hit. [Graphical view]
PIRSF	PIRSF000290. PPO_plant. 1 hit.
PRINTS	PR00092. TYROSINASE.
SUPFAM	SSF48056. Di-copper_centre. 1 hit.
PROSITE	PS00497. TYROSINASE_1. 1 hit. PS00498. TYROSINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPOD_SOLLC

Accession

Primary (citable) accession number: Q08306

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program