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Q08304 (PPOB_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyphenol oxidase B, chloroplastic

Short name=PPO
EC=1.10.3.1
Alternative name(s):
Catechol oxidase
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length596 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.

Catalytic activity

2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.

Cofactor

Binds 2 copper ions per subunit By similarity.

Subcellular location

Plastidchloroplast thylakoid lumen.

Sequence similarities

Belongs to the tyrosinase family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
Thylakoid
   DomainTransit peptide
   LigandCopper
Metal-binding
   Molecular functionOxidoreductase
   PTMDisulfide bond
Thioether bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpigment biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast thylakoid lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncatechol oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 8787Chloroplast By similarity
Chain88 – 596509Polyphenol oxidase B, chloroplastic
PRO_0000035911

Sites

Metal binding1811Copper A By similarity
Metal binding1991Copper A By similarity
Metal binding2081Copper A By similarity
Metal binding3291Copper B By similarity
Metal binding3331Copper B By similarity
Metal binding3711Copper B By similarity

Amino acid modifications

Disulfide bond98 ↔ 114 By similarity
Disulfide bond113 ↔ 182 By similarity
Cross-link185 ↔ 1992'-(S-cysteinyl)-histidine (Cys-His) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q08304 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: BD8553F7972FC7E8

FASTA59667,227
        10         20         30         40         50         60 
MASVVCNSSS STTTTTLKTP FTSLGSTPKP SQLFLHGKRN KTFKVSCKVI NNNGNQDETN 

        70         80         90        100        110        120 
SVDRRNVLLG LGGLYGVANA IPLAASATPI PSPDLKTCGR ATISDGPLVP YSCCPPPMPT 

       130        140        150        160        170        180 
NFDTIPYYKF PSMTKLRIRT PAHAVDEEYI AKYNLAISRM RDLDKTEPLN PLGFKQQANI 

       190        200        210        220        230        240 
HCAYCNGAYI IGGKELQVHN SWLFFPFHRW YLYFYERILG KLIDDPTFAL PYWNWDHPKG 

       250        260        270        280        290        300 
MRLPPMFDRE GSSLYDERRN QQVRNGTVLD LGSFGDKVET TQLQLMSNNL TLMYRQMVTN 

       310        320        330        340        350        360 
APCPLLFFGA PYVLGNNVEA PGTIETIPHI PVHIWAGTVR GSKFPNGDVS YGEDMGNFYS 

       370        380        390        400        410        420 
AGLDPVFYCH HGNVDRMWNE WKAIGGKRRD ISEKDWLNSE FFFYDEHKNP YRVKVRDCLD 

       430        440        450        460        470        480 
TKKMGYDYAP MPTPWRNFKP KSKASVGKVN TSTLPPANEV FPLAKMDKTI SFAINRPASS 

       490        500        510        520        530        540 
RTQQEKNEQE EMLTFNNIRY DNRGYIRFDV FLNVDNNVNA NELDKAEFAG SYTSLPHVHR 

       550        560        570        580        590 
AGENDHIAKV NFQLAITELL EDIGLEDEDT IAVTLVPKKG GEGISIENVE IKLVDC 

« Hide

References

[1]"Organisation of the tomato polyphenol oxidase gene family."
Newman S.M., Eannetta N.T., Yu H., Prince J.P., de Vicente M.C., Tanksley S.D., Steffens J.C.
Plant Mol. Biol. 21:1035-1051(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. VFNT Cherry.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z12834 Genomic DNA. Translation: CAA78296.1.
PIRS33540.
RefSeqXP_004246032.1. XM_004245984.1.

3D structure databases

ProteinModelPortalQ08304.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101258774.
KEGGsly:101258774.

Family and domain databases

Gene3D1.10.1280.10. 1 hit.
InterProIPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase_Cu-bd.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFPIRSF000290. PPO_plant. 1 hit.
PRINTSPR00092. TYROSINASE.
SUPFAMSSF48056. SSF48056. 1 hit.
PROSITEPS00497. TYROSINASE_1. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPOB_SOLLC
AccessionPrimary (citable) accession number: Q08304
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families