Reviewed, UniProtKB/Swiss-Prot Q08304 (PPOB_SOLLC)

Names and origin

Protein names	Recommended name:     Polyphenol oxidase B, chloroplastic       Short name=PPO     EC=1.10.3.1 Alternative name(s):     Catechol oxidase
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.
Catalytic activity	2 catechol + O(2) = 2 1,2-benzoquinone + 2 H(2)O.
Cofactor	Binds 2 copper ions per subunit By similarity.
Subcellular location	Plastid › chloroplast thylakoid lumen.
Sequence similarities	Belongs to the tyrosinase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Thioether bond
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catechol oxidase activity Inferred from electronic annotation. Source: EC copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 87	87	Chloroplast By similarity
	Chain	88 – 596	509	Polyphenol oxidase B, chloroplastic		PRO_0000035911
Sites
	Metal binding	181	1	Copper A By similarity
	Metal binding	199	1	Copper A By similarity
	Metal binding	208	1	Copper A By similarity
	Metal binding	329	1	Copper B By similarity
	Metal binding	333	1	Copper B By similarity
	Metal binding	371	1	Copper B By similarity
Amino acid modifications
	Disulfide bond	98 ↔ 114		By similarity
	Disulfide bond	113 ↔ 182		By similarity
	Cross-link	185 ↔ 199		2'-(S-cysteinyl)-histidine (Cys-His) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08304-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: BD8553F7972FC7E8	FASTA	596	67,227
	10 20 30 40 50 60 MASVVCNSSS STTTTTLKTP FTSLGSTPKP SQLFLHGKRN KTFKVSCKVI NNNGNQDETN 70 80 90 100 110 120 SVDRRNVLLG LGGLYGVANA IPLAASATPI PSPDLKTCGR ATISDGPLVP YSCCPPPMPT 130 140 150 160 170 180 NFDTIPYYKF PSMTKLRIRT PAHAVDEEYI AKYNLAISRM RDLDKTEPLN PLGFKQQANI 190 200 210 220 230 240 HCAYCNGAYI IGGKELQVHN SWLFFPFHRW YLYFYERILG KLIDDPTFAL PYWNWDHPKG 250 260 270 280 290 300 MRLPPMFDRE GSSLYDERRN QQVRNGTVLD LGSFGDKVET TQLQLMSNNL TLMYRQMVTN 310 320 330 340 350 360 APCPLLFFGA PYVLGNNVEA PGTIETIPHI PVHIWAGTVR GSKFPNGDVS YGEDMGNFYS 370 380 390 400 410 420 AGLDPVFYCH HGNVDRMWNE WKAIGGKRRD ISEKDWLNSE FFFYDEHKNP YRVKVRDCLD 430 440 450 460 470 480 TKKMGYDYAP MPTPWRNFKP KSKASVGKVN TSTLPPANEV FPLAKMDKTI SFAINRPASS 490 500 510 520 530 540 RTQQEKNEQE EMLTFNNIRY DNRGYIRFDV FLNVDNNVNA NELDKAEFAG SYTSLPHVHR 550 560 570 580 590 AGENDHIAKV NFQLAITELL EDIGLEDEDT IAVTLVPKKG GEGISIENVE IKLVDC

References

[1]	"Organisation of the tomato polyphenol oxidase gene family." Newman S.M., Eannetta N.T., Yu H., Prince J.P., de Vicente M.C., Tanksley S.D., Steffens J.C. Plant Mol. Biol. 21:1035-1051(1993) [PubMed: 8098228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. VFNT Cherry.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z12834 Genomic DNA. Translation: CAA78296.1.
PIR	S33540.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR008922. Di-copper_centre. IPR016213. Polyphenol_Oxase_pln. IPR002227. Tyrosinase. [Graphical view]
Gene3D	G3DSA:1.10.1280.10. Di-copper_centre. 1 hit.
Pfam	PF00264. Tyrosinase. 1 hit. [Graphical view]
PIRSF	PIRSF000290. PPO_plant. 1 hit.
PRINTS	PR00092. TYROSINASE.
PROSITE	PS00497. TYROSINASE_1. 1 hit. PS00498. TYROSINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPOB_SOLLC

Accession

Primary (citable) accession number: Q08304

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 4, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)