Reviewed, UniProtKB/Swiss-Prot Q082Q2 (KATG1_SHEFN)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	Sfri_1917

Organism

Shewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]

Taxonomic identifier

318167 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 728	712	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354918
Sites
	Active site	98	1	Proton acceptor By similarity
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Site	94	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	97 ↔ 225		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity
	Cross-link	225 ↔ 251		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-97) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q082Q2-1 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 8CDEEEF3A613CB6C	FASTA	728	81,314
	10 20 30 40 50 60 MDKAQHTQGK CPVAHGGNTT VASDVMEWWP NALNLDILHQ HDNKTNPMDP NFDYREAFKS 70 80 90 100 110 120 LDLSAVKQDL RALMTDSKDW WPADWGHYGG LMIRMAWHSA GTYRMADGRG GAGTGNQRFA 130 140 150 160 170 180 PLNSWPDNAN LDKARRLLWP IKKKYGNKLS WADLIILAGT IAYESMGLKT FGFAGGRADI 190 200 210 220 230 240 WHPEKDIYWG SEKQWLAASD AENSRYSGQR DLENPLAAVM MGLIYVNPEG VDGQPDPLKT 250 260 270 280 290 300 AQDIRVTFER MAMNDEETVA LTAGGHTVGK CHGNGRAENL EAAPEGAELE DQGLGWLNKT 310 320 330 340 350 360 SRGIGRDTVT SGIEGAWTTH PTQWDNGYFE LLLNYDWELK KSPAGAWQWQ PINIKEEHKP 370 380 390 400 410 420 VDVEDPSIRL SPIMTDADMA MKMDPEYRKI SDRFYQDPAY FSEVFARAWF KLTHRDLGPK 430 440 450 460 470 480 SRYLGTDVPA EELIWQDPIP QVDYSLTEQE VTDIKAKILA SGLSIAQLVA TAWDSARTFR 490 500 510 520 530 540 GSDFRGGANG ARIRLAPQKD WQGNEPARLQ KVLAVLATIQ AGLTKSVSIA DLIVLGGTAA 550 560 570 580 590 600 VEKAAHAAGV HVKVPFAAGR GDSTLAQTDV ESFDVLEPLH DAFRNWQKKD YVVQPEEMML 610 620 630 640 650 660 DRTQLMGLTA HEMTVLVGGM RVLGANYDNS KHGVFTDNVG VLSNDFFVNL TDMSYNWKPA 670 680 690 700 710 720 GKNLYHIVDR STDKVKWTAT RVDLVFGSNS ILRSYAEIYA QDDAKEKFVN DFVKTWTKVM NADRFDLM

References

[1]

"Complete sequence of Shewanella frigidimarina NCIMB 400." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Fredrickson J.K., Kolker E., McCuel L.A., DiChristina T., Nealson K.H., Newman D., Tiedje J.M. , Zhou J., Romine M.F., Culley D.E., Serres M., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000447 Genomic DNA. Translation: ABI71763.1. Different initiation.
RefSeq	YP_750601.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4278829.
GenomeReviews	Gene locus Sfri_1917 in contig CP000447_GR.
KEGG	sfr:Sfri_1917.
NMPDR	fig|318167.10.peg.1854.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q082Q2.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_SHEFN

Accession

Primary (citable) accession number: Q082Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	June 16, 2009
This is version 21 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)