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Protein

Siderophore iron transporter ENB1

Gene

ENB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the transport of siderophore enterobactin and so has a role in iron homeostasis.1 Publication

GO - Molecular functioni

  • ferric-enterobactin transmembrane transporter activity Source: SGD

GO - Biological processi

  • cellular iron ion homeostasis Source: GO_Central
  • ferric-enterobactin transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciYEAST:G3O-33546-MONOMER.

Protein family/group databases

TCDBi2.A.1.16.2. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Siderophore iron transporter ENB1
Alternative name(s):
Enterobactin permease
Siderophore iron transporter ARN4
Gene namesi
Name:ENB1
Synonyms:ARN4
Ordered Locus Names:YOL158C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL158C.
SGDiS000005518. ENB1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 140140CytoplasmicSequence analysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Topological domaini162 – 18726LumenalSequence analysisAdd
BLAST
Transmembranei188 – 20821HelicalSequence analysisAdd
BLAST
Topological domaini209 – 22416CytoplasmicSequence analysisAdd
BLAST
Transmembranei225 – 24521HelicalSequence analysisAdd
BLAST
Topological domaini246 – 27934LumenalSequence analysisAdd
BLAST
Transmembranei280 – 30021HelicalSequence analysisAdd
BLAST
Topological domaini301 – 31010CytoplasmicSequence analysis
Transmembranei311 – 33121HelicalSequence analysisAdd
BLAST
Topological domaini332 – 3376LumenalSequence analysis
Transmembranei338 – 35821HelicalSequence analysisAdd
BLAST
Topological domaini359 – 3602CytoplasmicSequence analysis
Transmembranei361 – 38121HelicalSequence analysisAdd
BLAST
Topological domaini382 – 3909LumenalSequence analysis
Transmembranei391 – 41020HelicalSequence analysisAdd
BLAST
Topological domaini411 – 4144CytoplasmicSequence analysis
Transmembranei415 – 43218HelicalSequence analysisAdd
BLAST
Topological domaini433 – 4353LumenalSequence analysis
Transmembranei436 – 45621HelicalSequence analysisAdd
BLAST
Topological domaini457 – 48024CytoplasmicSequence analysisAdd
BLAST
Transmembranei481 – 50121HelicalSequence analysisAdd
BLAST
Topological domaini502 – 55049LumenalSequence analysisAdd
BLAST
Transmembranei551 – 57121HelicalSequence analysisAdd
BLAST
Topological domaini572 – 60635CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cellular bud membrane Source: SGD
  • endosome Source: GO_Central
  • endosome membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
  • vacuolar membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606Siderophore iron transporter ENB1PRO_0000084865Add
BLAST

Proteomic databases

MaxQBiQ08299.

PTM databases

iPTMnetiQ08299.

Interactioni

Protein-protein interaction databases

BioGridi34260. 9 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ08299.
SMRiQ08299. Positions 554-579.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000002930.
InParanoidiQ08299.
KOiK08197.
OMAiSSTNWQF.
OrthoDBiEOG7PZS6Z.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLETDHSRND NLDDKSTVCY SEKTDSNVEK STTSGLRRID AVNKVLSDYS
60 70 80 90 100
SFTAFGVTFS SLKTALLVAL FLQGYCTGLG GQISQSIQTY AANSFGKHSQ
110 120 130 140 150
VGSINTVKSI VASVVAVPYA RISDRFGRIE CWIFALVLYT IGEIISAATP
160 170 180 190 200
TFSGLFAGIV IQQFGYSGFR LLATALTGDL SGLRDRTFAM NIFLIPVIIN
210 220 230 240 250
TWVSGNIVSS VAGNVAPYKW RWGYGIFCII VPISTLILVL PYVYAQYISW
260 270 280 290 300
RSGKLPPLKL KEKGQTLRQT LWKFADDINL IGVILFTAFL VLVLLPLTIA
310 320 330 340 350
GGATSKWREG HIIAMIVVGG CLGFIFLIWE LKFAKNPFIP RVYLGDPTIY
360 370 380 390 400
VALLMEFVWR LGLQIELEYL VTVLMVAFGE STLSAQRIAQ LYNFLQSCTN
410 420 430 440 450
IVVGIMLHFY PHPKVFVVAG SLLGVIGMGL LYKYRVVYDG ISGLIGAEIV
460 470 480 490 500
VGIAGGMIRF PMWTLVHAST THNEMATVTG LLMSVYQIGD AVGASIAGAI
510 520 530 540 550
WTQRLAKELI QRLGSSLGMA IYKSPLNYLK KYPIGSEVRV QMIESYSKIQ
560 570 580 590 600
RLLIIVSISF AAFNAVLCFF LRGFTVNKKQ SLSAEEREKE KLKIKQQSWL

RRVIGY
Length:606
Mass (Da):66,970
Last modified:November 1, 1996 - v1
Checksum:i4ED81A2A6A43815D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74900 Genomic DNA. Translation: CAA99180.1.
BK006948 Genomic DNA. Translation: DAA10628.1.
PIRiS66857.
RefSeqiNP_014484.1. NM_001183411.1.

Genome annotation databases

EnsemblFungiiYOL158C; YOL158C; YOL158C.
GeneIDi854007.
KEGGisce:YOL158C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74900 Genomic DNA. Translation: CAA99180.1.
BK006948 Genomic DNA. Translation: DAA10628.1.
PIRiS66857.
RefSeqiNP_014484.1. NM_001183411.1.

3D structure databases

ProteinModelPortaliQ08299.
SMRiQ08299. Positions 554-579.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34260. 9 interactions.

Protein family/group databases

TCDBi2.A.1.16.2. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ08299.

Proteomic databases

MaxQBiQ08299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL158C; YOL158C; YOL158C.
GeneIDi854007.
KEGGisce:YOL158C.

Organism-specific databases

EuPathDBiFungiDB:YOL158C.
SGDiS000005518. ENB1.

Phylogenomic databases

GeneTreeiENSGT00390000002930.
InParanoidiQ08299.
KOiK08197.
OMAiSSTNWQF.
OrthoDBiEOG7PZS6Z.

Enzyme and pathway databases

BioCyciYEAST:G3O-33546-MONOMER.

Miscellaneous databases

PROiQ08299.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake."
    Yun C.-W., Ferea T., Rashford J., Ardon O., Brown P.O., Botstein D., Kaplan J., Philpott C.C.
    J. Biol. Chem. 275:10709-10715(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The response to iron deprivation in Saccharomyces cerevisiae: expression of siderophore-based systems of iron uptake."
    Philpott C.C., Protchenko O., Kim Y.W., Boretsky Y., Shakoury-Elizeh M.
    Biochem. Soc. Trans. 30:698-702(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiENB1_YEAST
AccessioniPrimary (citable) accession number: Q08299
Secondary accession number(s): D6W1R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.