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Q08297

- RAD51_MOUSE

UniProt

Q08297 - RAD51_MOUSE

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Protein

DNA repair protein RAD51 homolog 1

Gene

Rad51

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi127 – 1348ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: MGI
  2. damaged DNA binding Source: InterPro
  3. double-stranded DNA binding Source: Ensembl
  4. single-stranded DNA binding Source: Ensembl
  5. single-stranded DNA-dependent ATPase activity Source: Ensembl

GO - Biological processi

  1. cellular response to camptothecin Source: UniProtKB
  2. cellular response to DNA damage stimulus Source: UniProtKB
  3. cellular response to ionizing radiation Source: UniProtKB
  4. DNA repair Source: UniProtKB
  5. DNA unwinding involved in DNA replication Source: UniProtKB
  6. double-strand break repair via homologous recombination Source: MGI
  7. meiotic nuclear division Source: MGI
  8. positive regulation of DNA ligation Source: Ensembl
  9. protein homooligomerization Source: Ensembl
  10. reciprocal meiotic recombination Source: UniProtKB
  11. regulation of double-strand break repair via homologous recombination Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198629. Meiotic recombination.
REACT_235085. Presynaptic phase of homologous DNA pairing and strand exchange.
REACT_261855. Assembly of the RAD51-ssDNA nucleoprotein complex.
REACT_27235. Meiotic Recombination.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD51 homolog 1
Alternative name(s):
RAD51 homolog A
Gene namesi
Name:Rad51
Synonyms:Rad51a, Reca
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:97890. Rad51.

Subcellular locationi

Nucleus. Cytoplasm By similarity. Cytoplasmperinuclear region By similarity. Mitochondrion matrix By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
Note: Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner (By similarity).By similarity

GO - Cellular componenti

  1. condensed chromosome Source: MGI
  2. condensed nuclear chromosome Source: MGI
  3. cytoplasm Source: UniProtKB
  4. cytoskeleton Source: UniProtKB-KW
  5. lateral element Source: MGI
  6. mitochondrion Source: UniProtKB-KW
  7. nuclear chromosome Source: UniProtKB
  8. nucleolus Source: Ensembl
  9. nucleoplasm Source: Reactome
  10. nucleus Source: UniProtKB
  11. perinuclear region of cytoplasm Source: UniProtKB
  12. PML body Source: Ensembl
  13. synaptonemal complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 339338DNA repair protein RAD51 homolog 1PRO_0000122933Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei54 – 541Phosphotyrosine; by ABL1By similarity
Modified residuei309 – 3091Phosphothreonine; by CHEK1By similarity

Post-translational modificationi

Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ08297.
PaxDbiQ08297.
PRIDEiQ08297.

PTM databases

PhosphoSiteiQ08297.

Expressioni

Tissue specificityi

Expressed in ovary and testis. Expressed in the brain.1 Publication

Developmental stagei

Expression in the brain is strongest at day E12, particularly in the cortical ventricular zone. In the cortex of newborn mice (P0), RAD51 is mainly present in the subplate and, in lesser amounts, in layer V. It is detected in a subpopulation of corticospinal axons at the pyramidal decussation in 2-day-old (P2) mice.1 Publication

Gene expression databases

BgeeiQ08297.
CleanExiMM_RAD51.
ExpressionAtlasiQ08297. baseline and differential.
GenevestigatoriQ08297.

Interactioni

Subunit structurei

Forms linear homooligomers, giving rise to a RAD51 nucleoprotein filament, which is essential for strand-pairing reactions during DNA recombination. Interacts with BRCA1 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Interacts with the BCDX2 subcomplex RAD51C:RAD51B. Interacts directly with PALB2 which may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3. Interacts with RAD51AP1 and RAD51AP2. Interacts with CHEK1, and this may require prior phosphorylation of CHEK1. Interacts with the MND1-PSMC3IP heterodimer. Found in a complex, at least composed of BLM, RAD51 and SPIDR; the complex formation is mediated by SPIDR. Interacts with SPIDR; the interaction is direct and recruits RAD51 to DNA damage sites. Interacts with FIGNL1 (via N-terminal one-half region); the interaction is direct. Interacts with RAD51AP1 (via C-terminal region); the interaction is direct. Interacts with NABP2, RPA1, PALB2 and RAD51. Interacts with SWI5/C9orf119, and at lower level with SFR1/MEIR5. Interacts with hyperphosphorylated RPA2; this interaction is necessary for efficient recruitment to chromatin in response to DNA damage. Interacts with SWSAP1; involved in homologous recombination repair. Interacts with PARPBP, BRCA2 and RECQL5; these interactions interfere with the formation of the RAD51-DNA homologous recombination structure.2 Publications

Protein-protein interaction databases

BioGridi202564. 5 interactions.
IntActiQ08297. 1 interaction.
MINTiMINT-205669.

Structurei

3D structure databases

ProteinModelPortaliQ08297.
SMRiQ08297. Positions 16-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini48 – 7730HhHAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni184 – 25774Interaction with PALB2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated
Contains 1 HhH domain.Curated

Phylogenomic databases

eggNOGiCOG0468.
GeneTreeiENSGT00770000120539.
HOGENOMiHOG000227426.
HOVERGENiHBG001504.
InParanoidiQ08297.
KOiK04482.
OMAiMMAESRY.
PhylomeDBiQ08297.
TreeFamiTF101218.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02239. recomb_RAD51. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08297-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMQMQLEAS ADTSVEEESF GPQPISRLEQ CGINANDVKK LEEAGYHTVE
60 70 80 90 100
AVAYAPKKEL INIKGISEAK ADKILTEAAK LVPMGFTTAT EFHQRRSEII
110 120 130 140 150
QITTGSKELD KLLQGGIETG SITEMFGEFR TGKTQICHTL AVTCQLPIDR
160 170 180 190 200
GGGEGKAMYI DTEGTFRPER LLAVAERYGL SGSDVLDNVA YARGFNTDHQ
210 220 230 240 250
TQLLYQASAM MVESRYALLI VDSATALYRT DYSGRGELSA RQMHLARFLR
260 270 280 290 300
MLLRLADEFG VAVVITNQVV AQVDGAAMFA ADPKKPIGGN IIAHASTTRL
310 320 330
YLRKGRGETR ICKIYDSPCL PEAEAMFAIN ADGVGDAKD
Length:339
Mass (Da):36,971
Last modified:October 1, 1994 - v1
Checksum:iCE631E2C246BA481
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13473 mRNA. Translation: BAA02718.1.
D13803 mRNA. Translation: BAA02961.1.
AK011242 mRNA. Translation: BAB27489.1.
AK076468 mRNA. Translation: BAC36357.1.
AK151157 mRNA. Translation: BAE30162.1.
AK151177 mRNA. Translation: BAE30179.1.
BC027384 mRNA. Translation: AAH27384.1.
CCDSiCCDS16590.1.
PIRiA48221.
RefSeqiNP_035364.1. NM_011234.4.
UniGeneiMm.330492.

Genome annotation databases

EnsembliENSMUST00000028795; ENSMUSP00000028795; ENSMUSG00000027323.
GeneIDi19361.
KEGGimmu:19361.
UCSCiuc008ltd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13473 mRNA. Translation: BAA02718.1 .
D13803 mRNA. Translation: BAA02961.1 .
AK011242 mRNA. Translation: BAB27489.1 .
AK076468 mRNA. Translation: BAC36357.1 .
AK151157 mRNA. Translation: BAE30162.1 .
AK151177 mRNA. Translation: BAE30179.1 .
BC027384 mRNA. Translation: AAH27384.1 .
CCDSi CCDS16590.1.
PIRi A48221.
RefSeqi NP_035364.1. NM_011234.4.
UniGenei Mm.330492.

3D structure databases

ProteinModelPortali Q08297.
SMRi Q08297. Positions 16-339.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202564. 5 interactions.
IntActi Q08297. 1 interaction.
MINTi MINT-205669.

Chemistry

ChEMBLi CHEMBL2034808.

PTM databases

PhosphoSitei Q08297.

Proteomic databases

MaxQBi Q08297.
PaxDbi Q08297.
PRIDEi Q08297.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028795 ; ENSMUSP00000028795 ; ENSMUSG00000027323 .
GeneIDi 19361.
KEGGi mmu:19361.
UCSCi uc008ltd.1. mouse.

Organism-specific databases

CTDi 5888.
MGIi MGI:97890. Rad51.

Phylogenomic databases

eggNOGi COG0468.
GeneTreei ENSGT00770000120539.
HOGENOMi HOG000227426.
HOVERGENi HBG001504.
InParanoidi Q08297.
KOi K04482.
OMAi MMAESRY.
PhylomeDBi Q08297.
TreeFami TF101218.

Enzyme and pathway databases

Reactomei REACT_198629. Meiotic recombination.
REACT_235085. Presynaptic phase of homologous DNA pairing and strand exchange.
REACT_261855. Assembly of the RAD51-ssDNA nucleoprotein complex.
REACT_27235. Meiotic Recombination.

Miscellaneous databases

NextBioi 296421.
PROi Q08297.
SOURCEi Search...

Gene expression databases

Bgeei Q08297.
CleanExi MM_RAD51.
ExpressionAtlasi Q08297. baseline and differential.
Genevestigatori Q08297.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR011941. DNA_recomb/repair_Rad51.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view ]
Pfami PF08423. Rad51. 1 hit.
[Graphical view ]
PIRSFi PIRSF005856. Rad51. 1 hit.
SMARTi SM00382. AAA. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view ]
SUPFAMi SSF47794. SSF47794. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR02239. recomb_RAD51. 1 hit.
PROSITEi PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA."
    Shinohara A., Ogawa H., Matsuda Y., Ushio N., Ikeo K., Ogawa T.
    Nat. Genet. 4:239-243(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A mouse homolog of the Escherichia coli recA and Saccharomyces cerevisiae RAD51 genes."
    Morita T., Yoshimura Y., Yamamoto A., Murata K., Mori M., Yamamoto H., Matsushiro A.
    Proc. Natl. Acad. Sci. U.S.A. 90:6577-6580(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Analysis of mouse Rad54 expression and its implications for homologous recombination."
    Essers J., Hendriks R.W., Wesoly J., Beerens C.E.M.T., Smit B., Hoeijmakers J.H.J., Wyman C., Dronkert M.L.G., Kanaar R.
    DNA Repair 1:779-793(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: LEVEL OF PROTEIN EXPRESSION.
  6. "The Hop2 and Mnd1 proteins act in concert with Rad51 and Dmc1 in meiotic recombination."
    Petukhova G.V., Pezza R.J., Vanevski F., Ploquin M., Masson J.-Y., Camerini-Otero R.D.
    Nat. Struct. Mol. Biol. 12:449-453(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MND1-PSMC3IP HETERODIMER.
  7. "Role for the mammalian Swi5-Sfr1 complex in DNA strand break repair through homologous recombination."
    Akamatsu Y., Jasin M.
    PLoS Genet. 6:E1001160-E1001160(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SWI5.
  8. Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiRAD51_MOUSE
AccessioniPrimary (citable) accession number: Q08297
Secondary accession number(s): Q3UAY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 26, 2014
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The nucleus of a mouse embryonic stem (ES) cells contains on average 4.7 x 105 molecules.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3