Reviewed, UniProtKB/Swiss-Prot Q08296 (PPOF_SOLLC)

Names and origin

Protein names	Recommended name:     Polyphenol oxidase F, chloroplastic       Short name=PPO     EC=1.10.3.1 Alternative name(s):     Catechol oxidase
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of mono- and o-diphenols to o-diquinones.
Catalytic activity	2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.
Cofactor	Binds 2 copper ions per subunit By similarity.
Subcellular location	Plastid › chloroplast thylakoid lumen.
Sequence similarities	Belongs to the tyrosinase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid Thylakoid
Domain	Transit peptide
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond Thioether bond
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast thylakoid lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	catechol oxidase activity Inferred from electronic annotation. Source: EC copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 86	86	Chloroplast By similarity
	Chain	87 – 585	499	Polyphenol oxidase F, chloroplastic		PRO_0000035915
Sites
	Metal binding	179	1	Copper A By similarity
	Metal binding	197	1	Copper A By similarity
	Metal binding	206	1	Copper A By similarity
	Metal binding	326	1	Copper B By similarity
	Metal binding	330	1	Copper B By similarity
	Metal binding	361	1	Copper B By similarity
Amino acid modifications
	Disulfide bond	97 ↔ 114		By similarity
	Disulfide bond	113 ↔ 180		By similarity
	Cross-link	183 ↔ 197		2'-(S-cysteinyl)-histidine (Cys-His) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08296-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6C8C7C1D2F00DD5D	FASTA	585	66,183
	10 20 30 40 50 60 MSSSTPNTLP LLSTNKSLSS PFTNNHSTFL SKPSQPFLHG RRCQSFKVSC NVGEHDKNLD 70 80 90 100 110 120 AVDRRNVLLG LGGFYGAANL APLASAAPIP PPDLKSCGVA HIDDKGTEVS YSCCPPVPDD 130 140 150 160 170 180 IDSVPYYKFP PMTKLRIRPP AHAADEEYVA KYQLATSRMR ELDKDPFDPL GFKQQANIHC 190 200 210 220 230 240 AYCNGAYKIG GKELQVHFSW LFFPFHRWYL YFYERILGSL INDPTFALPY WNWDHPKGMR 250 260 270 280 290 300 IPPMFDREGS SLYDEKRNQN HRNGTIIDLG HFGKDVRTPQ LQIMTNNLTL MYRQMVTNAP 310 320 330 340 350 360 CPSQFFGAPL GSDPEPGMGT IENIPHTPVH IWTGDSPRQG HGEDMGNFYS AGLDPLFYCH 370 380 390 400 410 420 HANVDRMWNE WKLIGGKRRD LSNKDWLNSE FFFYDENRNP YRVKVRDCLD SKKMGFDYPP 430 440 450 460 470 480 MPTPWRNFKP IRRSSSGKVN TASIAPVSKV FPLAKLDRAI SFSLTRPASS RTTQEKNEQQ 490 500 510 520 530 540 EILTFNKMAY DDTKYVRFDV FLNVDKTVNA EELDKAEFAG SYTSLPHVHG NNDNHVKDVT 550 560 570 580 FTLAITELLE DIGLEDEDTI AVTLVPKVGG EGVSIESVEI KLEDC

References

[1]	"Organisation of the tomato polyphenol oxidase gene family." Newman S.M., Eannetta N.T., Yu H., Prince J.P., de Vicente M.C., Tanksley S.D., Steffens J.C. Plant Mol. Biol. 21:1035-1051(1993) [PubMed: 8098228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. VFNT Cherry.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z12838 Genomic DNA. Translation: CAA78300.1.
PIR	S33544.
3D structure databases
SMR	Q08296. Positions 87-426.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.10.3.1. 281054.
Family and domain databases
InterPro	IPR008922. Di-copper_centre. IPR016213. Polyphenol_Oxase_pln. IPR002227. Tyrosinase. [Graphical view]
Gene3D	G3DSA:1.10.1280.10. Di-copper_centre. 1 hit.
Pfam	PF00264. Tyrosinase. 1 hit. [Graphical view]
PIRSF	PIRSF000290. PPO_plant. 1 hit.
PRINTS	PR00092. TYROSINASE.
PROSITE	PS00497. TYROSINASE_1. 1 hit. PS00498. TYROSINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPOF_SOLLC

Accession

Primary (citable) accession number: Q08296

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 19, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)