Q08295 (IMA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Oligo-1,6-glucosidase IMA2
EC=3.2.1.10

Alternative name(s):
Alpha-glucosidase
Isomaltase 2

Gene names

Name:	IMA2
Ordered Locus Names:	YOL157C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Alpha-glucosidase with specificity for isomaltase, methyl-alpha-glucoside, and palatinose. Ref.4
Catalytic activity	Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.
Induction	By ethanol. Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Maltose metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	disaccharide catabolic process Inferred from genetic interaction PubMed 20471265Ref.4. Source: SGD maltose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cation binding Inferred from electronic annotation. Source: InterPro oligo-1,6-glucosidase activity Inferred from direct assay PubMed 20471265. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Oligo-1,6-glucosidase IMA2		PRO_0000245259
Sites
	Active site	215	1	Nucleophile By similarity
	Active site	277	1	Proton donor By similarity
	Site	352	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08295 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E2E2D77A2A77C75A	FASTA	589	68,625
	10 20 30 40 50 60 MTISSAHPET EPKWWKEATI YQIYPASFKD SNNDGWGDMK GIASKLEYIK ELGADAIWIS 70 80 90 100 110 120 PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL GMKFITDLVI NHCSSEHEWF 130 140 150 160 170 180 KESRSSKTNP KRDWFFWRPP KGYDAEGKPI PPNNWRSYFG GSAWTFDEKT QEFYLRLFCS 190 200 210 220 230 240 TQPDLNWENE DCRKAIYESA VGYWLDHGVD GFRIDVGSLY SKVAGLPDAP VIDENSKWQP 250 260 270 280 290 300 SDPFTMNGPR IHEFHQEMNK FIRNRVKDGR EIMTVGEMQH ATDETKRLYT SASRHELSEL 310 320 330 340 350 360 FNFSHTDVGT SPKFRQNLIP YELKDWKVAL AELFRYVNGT DCWSTIYLEN HDQPRSITRF 370 380 390 400 410 420 GDDSPKNRVI SGKLLSVLLV SLSGTLYVYQ GQELGEINFK NWPIEKYEDV EVRNNYDAIK 430 440 450 460 470 480 EEHGENSKEM KRFLEAIALI SRDHARTPMQ WSREEPNAGF SGPNAKPWFY LNESFREGIN 490 500 510 520 530 540 AEDESKDPNS VLNFWKEALR FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYDNKTLFAA 550 560 570 580 LNFSSDSIDF TIPNNSSSFK LEFGNYPRSE VDASSRTLKP WEGRIYISE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global gene expression during short-term ethanol stress in Saccharomyces cerevisiae." Alexandre H., Ansanay-Galeote V., Dequin S., Blondin B. FEBS Lett. 498:98-103(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[4]	"Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family." Teste M.A., Francois J.M., Parrou J.L. J. Biol. Chem. 285:26815-26824(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74899 Genomic DNA. Translation: CAA99179.1. BK006948 Genomic DNA. Translation: DAA10629.1.
PIR	S66856.
RefSeq	NP_014485.1. NM_001183410.1.
3D structure databases
ProteinModelPortal	Q08295.
SMR	Q08295. Positions 4-589.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q08295. 1 interaction.
STRING	4932.YOL157C.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
mycoCLAP	OGL13B_YEAST.
Proteomic databases
PaxDb	Q08295.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOL157C; YOL157C; YOL157C.
GeneID	854008.
KEGG	sce:YOL157C.
Organism-specific databases
CYGD	YOL157c.
SGD	S000005517. YOL157C.
Phylogenomic databases
eggNOG	COG0366.
GeneTree	ENSGT00530000063127.
KO	K01187.
OMA	WIDIPNN.
OrthoDB	EOG4K0TWQ.
Gene expression databases
ArrayExpress	Q08295.
Genevestigator	Q08295.
GermOnline	YOL157C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...
Other
NextBio	975518.

Entry information

Entry name

IMA2_YEAST

Accession

Primary (citable) accession number: Q08295
Secondary accession number(s): D6W1R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program