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Q08295

- IMA2_YEAST

UniProt

Q08295 - IMA2_YEAST

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Protein

Oligo-1,6-glucosidase IMA2

Gene
IMA2, YOL157C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Alpha-glucosidase with specificity for isomaltase, methyl-alpha-glucoside, and palatinose.1 Publication

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei215 – 2151Nucleophile By similarity
Active sitei277 – 2771Proton donor By similarity
Sitei352 – 3521Transition state stabilizer By similarity

GO - Molecular functioni

  1. cation binding Source: InterPro
  2. oligo-1,6-glucosidase activity Source: SGD

GO - Biological processi

  1. disaccharide catabolic process Source: SGD
  2. maltose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Maltose metabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-33545-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.
mycoCLAPiOGL13B_YEAST.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase IMA2 (EC:3.2.1.10)
Alternative name(s):
Alpha-glucosidase
Isomaltase 2
Gene namesi
Name:IMA2
Ordered Locus Names:YOL157C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL157c.
SGDiS000005517. IMA2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 589588Oligo-1,6-glucosidase IMA2PRO_0000245259Add
BLAST

Proteomic databases

PaxDbiQ08295.

Expressioni

Inductioni

By ethanol.1 Publication

Gene expression databases

GenevestigatoriQ08295.

Interactioni

Protein-protein interaction databases

BioGridi34261. 2 interactions.
IntActiQ08295. 1 interaction.
STRINGi4932.YOL157C.

Structurei

3D structure databases

ProteinModelPortaliQ08295.
SMRiQ08295. Positions 4-589.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0366.
GeneTreeiENSGT00530000063127.
KOiK01187.
OMAiLNMPAGR.
OrthoDBiEOG7M3J7X.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08295-1 [UniParc]FASTAAdd to Basket

« Hide

MTISSAHPET EPKWWKEATI YQIYPASFKD SNNDGWGDMK GIASKLEYIK    50
ELGADAIWIS PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL 100
GMKFITDLVI NHCSSEHEWF KESRSSKTNP KRDWFFWRPP KGYDAEGKPI 150
PPNNWRSYFG GSAWTFDEKT QEFYLRLFCS TQPDLNWENE DCRKAIYESA 200
VGYWLDHGVD GFRIDVGSLY SKVAGLPDAP VIDENSKWQP SDPFTMNGPR 250
IHEFHQEMNK FIRNRVKDGR EIMTVGEMQH ATDETKRLYT SASRHELSEL 300
FNFSHTDVGT SPKFRQNLIP YELKDWKVAL AELFRYVNGT DCWSTIYLEN 350
HDQPRSITRF GDDSPKNRVI SGKLLSVLLV SLSGTLYVYQ GQELGEINFK 400
NWPIEKYEDV EVRNNYDAIK EEHGENSKEM KRFLEAIALI SRDHARTPMQ 450
WSREEPNAGF SGPNAKPWFY LNESFREGIN AEDESKDPNS VLNFWKEALR 500
FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYDNKTLFAA LNFSSDSIDF 550
TIPNNSSSFK LEFGNYPRSE VDASSRTLKP WEGRIYISE 589
Length:589
Mass (Da):68,625
Last modified:November 1, 1996 - v1
Checksum:iE2E2D77A2A77C75A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74899 Genomic DNA. Translation: CAA99179.1.
BK006948 Genomic DNA. Translation: DAA10629.1.
PIRiS66856.
RefSeqiNP_014485.1. NM_001183410.1.

Genome annotation databases

EnsemblFungiiYOL157C; YOL157C; YOL157C.
GeneIDi854008.
KEGGisce:YOL157C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74899 Genomic DNA. Translation: CAA99179.1 .
BK006948 Genomic DNA. Translation: DAA10629.1 .
PIRi S66856.
RefSeqi NP_014485.1. NM_001183410.1.

3D structure databases

ProteinModelPortali Q08295.
SMRi Q08295. Positions 4-589.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34261. 2 interactions.
IntActi Q08295. 1 interaction.
STRINGi 4932.YOL157C.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.
mycoCLAPi OGL13B_YEAST.

Proteomic databases

PaxDbi Q08295.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL157C ; YOL157C ; YOL157C .
GeneIDi 854008.
KEGGi sce:YOL157C.

Organism-specific databases

CYGDi YOL157c.
SGDi S000005517. IMA2.

Phylogenomic databases

eggNOGi COG0366.
GeneTreei ENSGT00530000063127.
KOi K01187.
OMAi LNMPAGR.
OrthoDBi EOG7M3J7X.

Enzyme and pathway databases

BioCyci YEAST:G3O-33545-MONOMER.

Miscellaneous databases

NextBioi 975518.

Gene expression databases

Genevestigatori Q08295.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
[Graphical view ]
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Global gene expression during short-term ethanol stress in Saccharomyces cerevisiae."
    Alexandre H., Ansanay-Galeote V., Dequin S., Blondin B.
    FEBS Lett. 498:98-103(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  4. "Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family."
    Teste M.A., Francois J.M., Parrou J.L.
    J. Biol. Chem. 285:26815-26824(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiIMA2_YEAST
AccessioniPrimary (citable) accession number: Q08295
Secondary accession number(s): D6W1R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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