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Protein

Oligo-1,6-glucosidase IMA2

Gene

IMA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Alpha-glucosidase with specificity for isomaltase, methyl-alpha-glucoside, and palatinose.1 Publication

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in some oligosaccharides produced from starch and glycogen by alpha-amylase, and in isomaltose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei215 – 2151NucleophileBy similarity
Active sitei277 – 2771Proton donorBy similarity
Sitei352 – 3521Transition state stabilizerBy similarity

GO - Molecular functioni

  • oligo-1,6-glucosidase activity Source: SGD
  • sucrose alpha-glucosidase activity Source: SGD

GO - Biological processi

  • disaccharide catabolic process Source: SGD
  • maltose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Maltose metabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-33545-MONOMER.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.
mycoCLAPiOGL13B_YEAST.

Names & Taxonomyi

Protein namesi
Recommended name:
Oligo-1,6-glucosidase IMA2 (EC:3.2.1.10)
Alternative name(s):
Alpha-glucosidase
Isomaltase 2
Gene namesi
Name:IMA2
Ordered Locus Names:YOL157C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL157C.
SGDiS000005517. IMA2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 589588Oligo-1,6-glucosidase IMA2PRO_0000245259Add
BLAST

Expressioni

Inductioni

By ethanol.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi34261. 2 interactions.
IntActiQ08295. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ08295.
SMRiQ08295. Positions 4-589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

GeneTreeiENSGT00840000131768.
InParanoidiQ08295.
KOiK01182.
OMAiVEARTIY.
OrthoDBiEOG092C12JX.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTISSAHPET EPKWWKEATI YQIYPASFKD SNNDGWGDMK GIASKLEYIK
60 70 80 90 100
ELGADAIWIS PFYDSPQDDM GYDIANYEKV WPTYGTNEDC FALIEKTHKL
110 120 130 140 150
GMKFITDLVI NHCSSEHEWF KESRSSKTNP KRDWFFWRPP KGYDAEGKPI
160 170 180 190 200
PPNNWRSYFG GSAWTFDEKT QEFYLRLFCS TQPDLNWENE DCRKAIYESA
210 220 230 240 250
VGYWLDHGVD GFRIDVGSLY SKVAGLPDAP VIDENSKWQP SDPFTMNGPR
260 270 280 290 300
IHEFHQEMNK FIRNRVKDGR EIMTVGEMQH ATDETKRLYT SASRHELSEL
310 320 330 340 350
FNFSHTDVGT SPKFRQNLIP YELKDWKVAL AELFRYVNGT DCWSTIYLEN
360 370 380 390 400
HDQPRSITRF GDDSPKNRVI SGKLLSVLLV SLSGTLYVYQ GQELGEINFK
410 420 430 440 450
NWPIEKYEDV EVRNNYDAIK EEHGENSKEM KRFLEAIALI SRDHARTPMQ
460 470 480 490 500
WSREEPNAGF SGPNAKPWFY LNESFREGIN AEDESKDPNS VLNFWKEALR
510 520 530 540 550
FRKAHKDITV YGYDFEFIDL DNKKLFSFTK KYDNKTLFAA LNFSSDSIDF
560 570 580
TIPNNSSSFK LEFGNYPRSE VDASSRTLKP WEGRIYISE
Length:589
Mass (Da):68,625
Last modified:November 1, 1996 - v1
Checksum:iE2E2D77A2A77C75A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74899 Genomic DNA. Translation: CAA99179.1.
BK006948 Genomic DNA. Translation: DAA10629.1.
PIRiS66856.
RefSeqiNP_014485.1. NM_001183410.1.

Genome annotation databases

EnsemblFungiiYOL157C; YOL157C; YOL157C.
GeneIDi854008.
KEGGisce:YOL157C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74899 Genomic DNA. Translation: CAA99179.1.
BK006948 Genomic DNA. Translation: DAA10629.1.
PIRiS66856.
RefSeqiNP_014485.1. NM_001183410.1.

3D structure databases

ProteinModelPortaliQ08295.
SMRiQ08295. Positions 4-589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34261. 2 interactions.
IntActiQ08295. 1 interaction.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.
mycoCLAPiOGL13B_YEAST.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL157C; YOL157C; YOL157C.
GeneIDi854008.
KEGGisce:YOL157C.

Organism-specific databases

EuPathDBiFungiDB:YOL157C.
SGDiS000005517. IMA2.

Phylogenomic databases

GeneTreeiENSGT00840000131768.
InParanoidiQ08295.
KOiK01182.
OMAiVEARTIY.
OrthoDBiEOG092C12JX.

Enzyme and pathway databases

BioCyciYEAST:G3O-33545-MONOMER.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.

Miscellaneous databases

PROiQ08295.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIMA2_YEAST
AccessioniPrimary (citable) accession number: Q08295
Secondary accession number(s): D6W1R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.