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Protein

Calponin-1

Gene

Cnn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

GO - Molecular functioni

  • actin binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calponin-1
Alternative name(s):
Basic calponin
Calponin H1, smooth muscle
Gene namesi
Name:Cnn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi621883. Cnn1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297Calponin-1PRO_0000204770Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481PhosphoserineCombined sources
Modified residuei170 – 1701Phosphothreonine; by ROCK2By similarity
Modified residuei175 – 1751Phosphoserine; by ROCK2By similarity
Modified residuei180 – 1801Phosphothreonine; by ROCK2By similarity
Modified residuei184 – 1841Phosphothreonine; by ROCK2By similarity
Modified residuei259 – 2591Phosphothreonine; by ROCK2By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ08290.
PRIDEiQ08290.

PTM databases

iPTMnetiQ08290.
PhosphoSiteiQ08290.

Expressioni

Tissue specificityi

Smooth muscle, and tissues containing significant amounts of smooth muscle.

Gene expression databases

GenevisibleiQ08290. RN.

Interactioni

Subunit structurei

Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.By similarity

GO - Molecular functioni

  • actin binding Source: RGD

Protein-protein interaction databases

BioGridi249316. 3 interactions.
IntActiQ08290. 1 interaction.
MINTiMINT-7898287.
STRINGi10116.ENSRNOP00000029782.

Structurei

3D structure databases

ProteinModelPortaliQ08290.
SMRiQ08290. Positions 13-142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 131104CHPROSITE-ProRule annotationAdd
BLAST
Repeati164 – 18926Calponin-like 1Add
BLAST
Repeati204 – 22926Calponin-like 2Add
BLAST
Repeati243 – 26826Calponin-like 3Add
BLAST

Sequence similaritiesi

Belongs to the calponin family.Curated
Contains 3 calponin-like repeats.PROSITE-ProRule annotation
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2046. Eukaryota.
COG5199. LUCA.
GeneTreeiENSGT00550000074447.
HOVERGENiHBG005186.
InParanoidiQ08290.
OMAiRQIFEPT.
OrthoDBiEOG71P2B3.
PhylomeDBiQ08290.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001997. Calponin/LIMCH1.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamiPF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
[Graphical view]
PRINTSiPR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08290-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGSNF
60 70 80 90 100
MDGLKDGIIL CEFINKLQPG SVKKVNESTQ NWHQLENIGN FIKAITKYGV
110 120 130 140 150
KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER
160 170 180 190 200
RFEPEKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD
210 220 230 240 250
QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN
260 270 280 290
KGASQRGMTV YGLPRQVYDP KYCLTPEYPE LDEPTHNHHP HNYYNSA
Length:297
Mass (Da):33,344
Last modified:October 1, 1994 - v1
Checksum:i80483631899BE025
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti97 – 971K → E in BAA03320 (PubMed:8359698).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71071 mRNA. Translation: CAA50397.1.
D14437 mRNA. Translation: BAA03320.1.
BC061809 mRNA. Translation: AAH61809.1.
PIRiS37640. JN0773.
RefSeqiNP_113935.1. NM_031747.1.
UniGeneiRn.31788.

Genome annotation databases

EnsembliENSRNOT00000029577; ENSRNOP00000029782; ENSRNOG00000027736.
GeneIDi65204.
KEGGirno:65204.
UCSCiRGD:621883. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71071 mRNA. Translation: CAA50397.1.
D14437 mRNA. Translation: BAA03320.1.
BC061809 mRNA. Translation: AAH61809.1.
PIRiS37640. JN0773.
RefSeqiNP_113935.1. NM_031747.1.
UniGeneiRn.31788.

3D structure databases

ProteinModelPortaliQ08290.
SMRiQ08290. Positions 13-142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249316. 3 interactions.
IntActiQ08290. 1 interaction.
MINTiMINT-7898287.
STRINGi10116.ENSRNOP00000029782.

PTM databases

iPTMnetiQ08290.
PhosphoSiteiQ08290.

Proteomic databases

PaxDbiQ08290.
PRIDEiQ08290.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029577; ENSRNOP00000029782; ENSRNOG00000027736.
GeneIDi65204.
KEGGirno:65204.
UCSCiRGD:621883. rat.

Organism-specific databases

CTDi1264.
RGDi621883. Cnn1.

Phylogenomic databases

eggNOGiKOG2046. Eukaryota.
COG5199. LUCA.
GeneTreeiENSGT00550000074447.
HOVERGENiHBG005186.
InParanoidiQ08290.
OMAiRQIFEPT.
OrthoDBiEOG71P2B3.
PhylomeDBiQ08290.

Miscellaneous databases

PROiQ08290.

Gene expression databases

GenevisibleiQ08290. RN.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001997. Calponin/LIMCH1.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamiPF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
[Graphical view]
PRINTSiPR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of gene markers of differentiated and proliferating vascular smooth muscle cells."
    Shanahan C.M., Weissberg P.L., Metcalfe J.C.
    Circ. Res. 73:193-204(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Aortic smooth muscle.
  2. "cDNA cloning and mRNA expression of calponin and SM22 in rat aorta smooth muscle cells."
    Nishida W., Kitami Y., Hiwada K.
    Gene 130:297-302(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Aortic smooth muscle.
  3. Takahashi K.
    Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pulmonary artery.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCNN1_RAT
AccessioniPrimary (citable) accession number: Q08290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 8, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.