Restriction of telomere capping protein 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q08281 (RTC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Restriction of telomere capping protein 1

Alternative name(s):
SEH-associated protein 2

Gene names

Name:	RTC1
Synonyms:	SEA2
Ordered Locus Names:	YOL138C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1341 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis. May be involved in a process influencing telomere capping. Ref.12 Ref.14
Subunit structure	Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1. Interacts with ribosomes. Ref.7 Ref.14
Subcellular location	Vacuole membrane; Peripheral membrane protein Ref.4 Ref.14.
Disruption phenotype	Leads to short telomeres. Ref.6
Miscellaneous	Present with 606 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the WD repeat RTC1 family. Contains 1 RING-type zinc finger. Contains 9 WD repeats.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane Vacuole
Domain	Repeat WD repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein import into peroxisome matrix Inferred from electronic annotation. Source: InterPro
Cellular_component	Seh1-associated complex Inferred from direct assay Ref.14. Source: SGD extrinsic to fungal-type vacuolar membrane Inferred from direct assay Ref.14. Source: SGD peroxisome Inferred from electronic annotation. Source: InterPro
Molecular_function	peroxisome matrix targeting signal-2 binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1341

1341

Restriction of telomere capping protein 1

PRO_0000223528

Regions

Repeat

207 – 248

WD 1

Repeat

256 – 296

WD 2

Repeat

305 – 342

WD 3

Repeat

367 – 406

WD 4

Repeat

439 – 486

WD 5

Repeat

489 – 527

WD 6

Repeat

843 – 883

WD 7

Repeat

1129 – 1169

WD 8

Repeat

1216 – 1255

WD 9

Zinc finger

1293 – 1335

RING-type; degenerate

Compositional bias

753 – 765

Poly-Asp

Amino acid modifications

Modified residue

Phosphoserine Ref.13

Modified residue

620

Phosphoserine Ref.13

Modified residue

801

Phosphoserine Ref.10

Modified residue

804

Phosphoserine Ref.10

Modified residue

946

Phosphoserine Ref.11 Ref.13

Modified residue

950

Phosphoserine Ref.11

Modified residue

1036

Phosphoserine Ref.9 Ref.13

Modified residue

1080

Phosphoserine Ref.13

Modified residue

1087

Phosphoserine Ref.13

Modified residue

1089

Phosphoserine Ref.8 Ref.13

Modified residue

1123

Phosphoserine Ref.8 Ref.9 Ref.13

Modified residue

1129

Phosphoserine Ref.13

Modified residue

1133

Phosphoserine Ref.13

Experimental info

Sequence conflict

393

C → S in CAA64732. Ref.1

Sequence conflict

393

C → S in CAA99159. Ref.2

Sequence conflict

548

D → G in CAA64732. Ref.1

Sequence conflict

548

D → G in CAA99159. Ref.2

Sequence conflict

1222

D → DV in CAA64732. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q08281 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 45B62E3D341397AA
Show »

FASTA

1,341

149,344

        10         20         30         40         50         60 
MSLSPHVENA SIPKGSTPIP KNRNVSSIGK GEFLGSSSSN NSSFRMNHYS NSGQPSVLDS 

        70         80         90        100        110        120 
IRRPNLTPTF SYSNGVYMPE SHRTSSFNDS YLPYDKNPYA KTTGSMSNKS NMKIKTKKNA 

       130        140        150        160        170        180 
INTNTRKSSG LIYTTKVDKE LSSIDKVNDP NINGLVCAGK THLGLYKFSP SDRSIKCVHD 

       190        200        210        220        230        240 
FITPNSNTST RGTTSLLPKL SKRTRQNKFS TIADVKTGFN NYKNCIAVCN NSTAISIYDL 

       250        260        270        280        290        300 
NKSSSIDNPL ITSLCEHTRS INSFDFNMVE SNLIISGGQD SCVKIWDLRS NKSKSSNRSD 

       310        320        330        340        350        360 
ISINTASDSI RDVKWMPGYN FASKNDQGSS TYGNLKSGYK FASIHDSGYL LKFDLRQPAQ 

       370        380        390        400        410        420 
YEKKLNAHTG PGLCLNWHPN QEYIATGGRD GKCCLWFVGD NANAAENTVL NYGNSPSLHA 

       430        440        450        460        470        480 
PNTSLNNSGS LAFPKLTINT GYPVTKLKFK PAYSSNIYNS LLGISSMGDE AEVRIYSLAR 

       490        500        510        520        530        540 
KYIPKHVLLS ETPSLGLVWW DENLIFNIDK GTRINGWDIN KEPTVLENLS KNTTTWRDLD 

       550        560        570        580        590        600 
GNGLLSVDQE IGSYEVVEPE LQPTSSTTCK KHPGTIKNPK NGNPENQGII GGIKKGFSHT 

       610        620        630        640        650        660 
GLTSFTPERP PTLKAGPTFS TKSLTLASGA SSFNSSSASL TSLTPQTENR EEIAIEPPCI 

       670        680        690        700        710        720 
ITLDIPQIFN NIRLTKIAHS RKKNVISESS SMKNSPVEKF KYLARQLKFS YIREHNVSDS 

       730        740        750        760        770        780 
ADTAYKNDIE NIDVVKNATE THGDNTTTTN NNDDGDDDDD DDDDDKIIES HLLKKYNFPE 

       790        800        810        820        830        840 
NNTWATLMNE KVNNKKSKRN SSSSREFDEK DVRSSISSIS ASRQSHDRAR KIDKNVEAEL 

       850        860        870        880        890        900 
QEKIQTLVDL ISIATHNASV YLSIDDLTNF KIWILIRDSL LWDLKWMTSS QISSDNASNM 

       910        920        930        940        950        960 
DANESSDFEA GENLKTGKEF PEEDGAGTSG AESLVEERPQ AFRANSDEPS DAEKKPVSKL 

       970        980        990       1000       1010       1020 
KEQLKNTEII PYAQPNEDSD EVLTKLKELQ NQRLESRTKM GETVSDDVII EEDEHEHQEE 

      1030       1040       1050       1060       1070       1080 
EQPHDSPTKS AQFHASPIAK SIPILQKREH RKSFIDTFML HSPNGYNGDT DIGNEDDNIS 

      1090       1100       1110       1120       1130       1140 
PRFTYNSVSP RSKVSSLQSY ATTTSQLETF KKLSSHTAPI IGSPRHAPSR PDSIGREQLS 

      1150       1160       1170       1180       1190       1200 
SSLTKKLAKC KKIIADPPWD TKKLIKQLYN QATETGNVVL TVNILFLFQT IYQITEIDIA 

      1210       1220       1230       1240       1250       1260 
KDAIAHFLLL LHRYELFGIA ADVLKYCPFE DIMGSEGDQS SIRLFCERCG ELITNESSKE 

      1270       1280       1290       1300       1310       1320 
KLRAEAQQTG NKKIMDKFGY WYCDSCKKKN TSCVLCERPL KKLTMVILPC GHEGHFQCIQ 

      1330       1340 
EWFLDENEQE CPGGCPGVAF I

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 12 801 bp fragment of the left arm of yeast chromosome XV containing a putative 6-phosphofructo-2-kinase gene, a gene for a possible glycophospholipid-anchored surface protein and six other open reading frames." Aldea M., Piedrafita L., Casas C., Casamayor A., Khalid H., Balcells L., Arino J., Herrero E. Yeast 12:1053-1058(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 393 AND 548. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length." Askree S.H., Yehuda T., Smolikov S., Gurevich R., Hawk J., Coker C., Krauskopf A., Kupiec M., McEachern M.J. Proc. Natl. Acad. Sci. U.S.A. 101:8658-8663(2004) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[7]	"Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes." Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J. Genes Dev. 20:1294-1307(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RIBOSOMES.
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1089 AND SER-1123, MASS SPECTROMETRY. Strain: YAL6B.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1036 AND SER-1123, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-801 AND SER-804, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-946 AND SER-950, MASS SPECTROMETRY.
[12]	"A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae." Addinall S.G., Downey M., Yu M., Zubko M.K., Dewar J., Leake A., Hallinan J., Shaw O., James K., Wilkinson D.J., Wipat A., Durocher D., Lydall D. Genetics 180:2251-2266(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86; SER-620; SER-946; SER-1036; SER-1080; SER-1087; SER-1089; SER-1123; SER-1129 AND SER-1133, MASS SPECTROMETRY.
[14]	"A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae." Dokudovskaya S., Waharte F., Schlessinger A., Pieper U., Devos D.P., Cristea I.M., Williams R., Salamero J., Chait B.T., Sali A., Field M.C., Rout M.P., Dargemont C. Mol. Cell. Proteomics 10:M110.006478.1-M110.006478.17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE SEA COMPLEX, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95465 Genomic DNA. Translation: CAA64732.1. Z74880 Genomic DNA. Translation: CAA99159.1. BK006948 Genomic DNA. Translation: DAA10647.2.
PIR	S66835.
RefSeq	NP_014503.2. NM_001183392.2.
3D structure databases
ProteinModelPortal	Q08281.
SMR	Q08281. Positions 203-290.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q08281. 7 interactions.
MINT	MINT-4502222.
STRING	4932.YOL138C.
Proteomic databases
PaxDb	Q08281.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOL138C; YOL138C; YOL138C.
GeneID	853982.
KEGG	sce:YOL138C.
Organism-specific databases
CYGD	YOL138c.
SGD	S000005498. RTC1.
Phylogenomic databases
eggNOG	NOG299301.
OMA	GGRDGKC.
OrthoDB	EOG4XWK64.
Gene expression databases
Genevestigator	Q08281.
GermOnline	YOL138C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 2 hits. 3.30.40.10. 1 hit.
InterPro	IPR025651. PEX7-like_WD_rpt. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
PANTHER	PTHR22850:SF4. PTHR22850:SF4. 1 hit.
Pfam	PF00400. WD40. 2 hits. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00320. WD40. 2 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 2 hits. PS50294. WD_REPEATS_REGION. 2 hits. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975449.

Entry information

Entry name

RTC1_YEAST

Accession

Primary (citable) accession number: Q08281
Secondary accession number(s): D6W1T1, Q92271

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	September 21, 2011
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents