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Q08280 (BSC6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bypass of stop codon protein 6
Gene names
Name:BSC6
Ordered Locus Names:YOL137W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable transporter.

Subcellular location

Golgi apparatus › cis-Golgi network membrane; Multi-pass membrane protein Probable Ref.6.

Miscellaneous

Present with 414 molecules/cell in log phase SD medium. Ref.7

Sequence similarities

Belongs to the major facilitator superfamily.

Ontologies

Keywords
   Biological processTransport
   Cellular componentGolgi apparatus
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtransmembrane transport

Inferred from electronic annotation. Source: InterPro

   Cellular componentGolgi apparatus

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 497497Bypass of stop codon protein 6
PRO_0000084864

Regions

Topological domain1 – 7272Lumenal Potential
Transmembrane73 – 9321Helical; Potential
Topological domain94 – 14451Cytoplasmic Potential
Transmembrane145 – 16521Helical; Potential
Topological domain166 – 1672Lumenal Potential
Transmembrane168 – 18821Helical; Potential
Topological domain189 – 20517Cytoplasmic Potential
Transmembrane206 – 22621Helical; Potential
Topological domain227 – 2326Lumenal Potential
Transmembrane233 – 25321Helical; Potential
Topological domain254 – 30047Cytoplasmic Potential
Transmembrane301 – 32121Helical; Potential
Topological domain322 – 34019Lumenal Potential
Transmembrane341 – 36121Helical; Potential
Topological domain362 – 37312Cytoplasmic Potential
Transmembrane374 – 39421Helical; Potential
Topological domain395 – 3973Lumenal Potential
Transmembrane398 – 41821Helical; Potential
Topological domain419 – 43921Cytoplasmic Potential
Transmembrane440 – 46021Helical; Potential
Topological domain461 – 4622Lumenal Potential
Transmembrane463 – 48321Helical; Potential
Topological domain484 – 49714Cytoplasmic Potential

Amino acid modifications

Modified residue331Phosphoserine Ref.11
Modified residue371Phosphoserine Ref.9 Ref.10 Ref.11
Modified residue411Phosphoserine Ref.9 Ref.10 Ref.11
Glycosylation491N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict4721C → R in AAT92920. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q08280 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 2458A5A8DA6BC731

FASTA49755,084
        10         20         30         40         50         60 
MDASSVPPKV DDYGMYTTEI SHHNPIELKN LLSSSDSRRN SQDEDSLPNN TNLIKEIDWQ 

        70         80         90        100        110        120 
GEKVKTYPLN YQTVPLVKLQ VIACLIMFVV FGMNDQTVGA LLPTLIEYYH ISRVDVSNVF 

       130        140        150        160        170        180 
IVQLCGYVMA SLSKERLNKH FGMRGGMLLA AGLCIVFLII LATAPSSFYV CMFCGLPLGL 

       190        200        210        220        230        240 
GIGILDSTGN VLMGSLLVHK NELMGIMHGL YGAAAMVTPP LVSYFVEWGH WSLFFLIPLF 

       250        260        270        280        290        300 
FSIIGMIVIF PAFKFETASK YDYLCSVENK ESNNDVEEAG DNSLMESTKA SPGFFELLRN 

       310        320        330        340        350        360 
PAIFLYSLYL FLYLGAEITT GSWFFSYLLE TKSSNKVAMS YIAASFWTGL TVGRLCLGFV 

       370        380        390        400        410        420 
TERFFENEYK ASKAYAFLTL SSYTLFVLVG LINSSSVFYF VVLFFVVFCC GTFIGPLFPN 

       430        440        450        460        470        480 
ASIVALQVLP KRLHVSGVGV AVAVGGCGGA AIPYLAGVIA HTVGIQYIPL LCWIMVALFT 

       490 
LEWTLYPKFI KGHEEYF

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 12 801 bp fragment of the left arm of yeast chromosome XV containing a putative 6-phosphofructo-2-kinase gene, a gene for a possible glycophospholipid-anchored surface protein and six other open reading frames."
Aldea M., Piedrafita L., Casas C., Casamayor A., Khalid H., Balcells L., Arino J., Herrero E.
Yeast 12:1053-1058(1996) [PubMed: 8896270] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed: 9169874] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Identification of stop codon readthrough genes in Saccharomyces cerevisiae."
Namy O., Duchateau-Nguyen G., Hatin I., Hermann-Le Denmat S., Termier M., Rousset J.-P.
Nucleic Acids Res. 31:2289-2296(2003) [PubMed: 12711673] [Abstract]
Cited for: GENE NAME.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37 AND SER-41, MASS SPECTROMETRY.
Strain: ADR376.
[10]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37 AND SER-41, MASS SPECTROMETRY.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33; SER-37 AND SER-41, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X95465 Genomic DNA. Translation: CAA64736.1.
Z74879 Genomic DNA. Translation: CAA99158.1.
AY692901 Genomic DNA. Translation: AAT92920.1.
BK006948 Genomic DNA. Translation: DAA10648.1.
PIRS66834.
RefSeqNP_014504.1. NM_001183391.1.

3D structure databases

ProteinModelPortalQ08280.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4683N.
IntActQ08280. 12 interactions.
MINTMINT-481943.
STRINGQ08280.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL137W; YOL137W; YOL137W.
GeneID853983.
KEGGsce:YOL137W.
NMPDRfig|4932.3.peg.5590.

Organism-specific databases

CYGDYOL137w.
SGDS000005497. BSC6.

Phylogenomic databases

eggNOGfuNOG08254.
GeneTreeEFGT00050000000650.
HOGENOMHBG202858.
OMAMGANDAA.
OrthoDBEOG4QRMD2.

Gene expression databases

ArrayExpressQ08280.
GenevestigatorQ08280.
GermOnlineYOL137W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975452.

Entry information

Entry nameBSC6_YEAST
AccessionPrimary (citable) accession number: Q08280
Secondary accession number(s): D6W1T2, Q6B229
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: December 14, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents