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Protein

Mediator of RNA polymerase II transcription subunit 7

Gene

MED7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.3 Publications

Miscellaneous

Present with 7598 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • negative regulation of transcription by RNA polymerase II Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33529-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 7
Alternative name(s):
Mediator complex subunit 7
Gene namesi
Name:MED7
Ordered Locus Names:YOL135C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL135C
SGDiS000005495 MED7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000963931 – 222Mediator of RNA polymerase II transcription subunit 7Add BLAST222

Proteomic databases

MaxQBiQ08278
PaxDbiQ08278
PRIDEiQ08278

PTM databases

iPTMnetiQ08278

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. MED7 interacts directly with MED1, MED4 and SRB7/MED21.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34241, 465 interactors
DIPiDIP-1435N
IntActiQ08278, 52 interactors
MINTiQ08278
STRINGi4932.YOL135C

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 22Combined sources5
Helixi25 – 39Combined sources15
Helixi61 – 65Combined sources5
Helixi74 – 76Combined sources3
Helixi112 – 133Combined sources22
Beta strandi135 – 137Combined sources3
Helixi141 – 143Combined sources3
Helixi144 – 164Combined sources21
Helixi166 – 203Combined sources38

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YKEX-ray3.30A/C102-205[»]
1YKHX-ray3.00A102-205[»]
3FBIX-ray2.80A/C2-83[»]
3FBNX-ray3.01A/C2-83[»]
5SVAelectron microscopy15.30U1-222[»]
ProteinModelPortaliQ08278
SMRiQ08278
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08278

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 7 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000064187
HOGENOMiHOG000000771
InParanoidiQ08278
KOiK15148
OMAiKRLYPQH
OrthoDBiEOG092C5KDU

Family and domain databases

InterProiView protein in InterPro
IPR037212 Med7/Med21-like
IPR009244 Mediatior_Med7
PANTHERiPTHR21428 PTHR21428, 1 hit
PfamiView protein in Pfam
PF05983 Med7, 1 hit
SUPFAMiSSF140718 SSF140718, 1 hit

Sequencei

Sequence statusi: Complete.

Q08278-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNDPGNEVS SLYPPPPPYV KFFTQSNLEK LPKYKEKKAA SAKQTAPNNS
60 70 80 90 100
NGGSEEEITC ALDYLIPPPM PKNQQYRAFG SIWQVKDQLP DLESMGLTQL
110 120 130 140 150
YKKSTENEST NYQYKIQELR KLLKSLLLNY LELIGVLSIN PDMYERKVEN
160 170 180 190 200
IRTILVNIHH LLNEYRPHQS RESLIMLLEE QLEYKRGEIR EIEQVCKQVH
210 220
DKLTSIQDTL RTGSQSPPSS SQ
Length:222
Mass (Da):25,585
Last modified:November 1, 1996 - v1
Checksum:iA88301E4EF3223C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95465 Genomic DNA Translation: CAA64734.1
Z74877 Genomic DNA Translation: CAA99156.1
AY692889 Genomic DNA Translation: AAT92908.1
BK006948 Genomic DNA Translation: DAA10650.1
PIRiS66832
RefSeqiNP_014506.1, NM_001183389.1

Genome annotation databases

EnsemblFungiiYOL135C; YOL135C; YOL135C
GeneIDi853985
KEGGisce:YOL135C

Similar proteinsi

Entry informationi

Entry nameiMED7_YEAST
AccessioniPrimary (citable) accession number: Q08278
Secondary accession number(s): D6W1T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health