Mediator of RNA polymerase II transcription subunit 7 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot Q08278 (MED7_YEAST)

Last modified November 24, 2009. Version 77. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 7
Alternative name(s):
Mediator complex subunit 7

Gene names

Name:	MED7
Ordered Locus Names:	YOL135C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. Ref.5 Ref.12 Ref.13
Subunit structure	Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. MED7 interacts directly with MED1, MED4 and SRB7/MED21. Ref.5 Ref.15
Subcellular location	Nucleus.
Miscellaneous	Present with 7598 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the Mediator complex subunit 7 family.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: InterPro transcription from RNA polymerase II promoter Ref.4 Inferred from direct assay. Source: SGD
Cellular component	mediator complex Ref.4 Inferred from direct assay. Source: SGD
Molecular function	RNA polymerase II transcription mediator activity Ref.4 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
FAR7	P43592	1	EBI-10674,EBI-22932
SOH1	P38633	1	EBI-10674,EBI-17658

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 222

222

Mediator of RNA polymerase II transcription subunit 7

PRO_0000096393

Secondary structure

222

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q08278-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A88301E4EF3223C9
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FASTA

222

25,585

        10         20         30         40         50         60 
MSNDPGNEVS SLYPPPPPYV KFFTQSNLEK LPKYKEKKAA SAKQTAPNNS NGGSEEEITC 

        70         80         90        100        110        120 
ALDYLIPPPM PKNQQYRAFG SIWQVKDQLP DLESMGLTQL YKKSTENEST NYQYKIQELR 

       130        140        150        160        170        180 
KLLKSLLLNY LELIGVLSIN PDMYERKVEN IRTILVNIHH LLNEYRPHQS RESLIMLLEE 

       190        200        210        220 
QLEYKRGEIR EIEQVCKQVH DKLTSIQDTL RTGSQSPPSS SQ

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 12 801 bp fragment of the left arm of yeast chromosome XV containing a putative 6-phosphofructo-2-kinase gene, a gene for a possible glycophospholipid-anchored surface protein and six other open reading frames." Aldea M., Piedrafita L., Casas C., Casamayor A., Khalid H., Balcells L., Arino J., Herrero E. Yeast 12:1053-1058(1996) [PubMed: 8896270] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain." Myers L.C., Gustafsson C.M., Bushnell D.A., Lui M., Erdjument-Bromage H., Tempst P., Kornberg R.D. Genes Dev. 12:45-54(1998) [PubMed: 9420330] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF MEDIATOR COMPLEX.
[5]	"The structural and functional organization of the yeast mediator complex." Kang J.S., Kim S.H., Hwang M.S., Han S.J., Lee Y.C., Kim Y.-J. J. Biol. Chem. 276:42003-42010(2001) [PubMed: 11555651] [Abstract] Cited for: INTERACTION WITH MED1; MED4 AND SRB7, FUNCTION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Association of the Mediator complex with enhancers of active genes." Kuras L., Borggrefe T., Kornberg R.D. Proc. Natl. Acad. Sci. U.S.A. 100:13887-13891(2003) [PubMed: 14623974] [Abstract] Cited for: ASSOCIATION WITH PROMOTER REGIONS.
[9]	"A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II." Bourbon H.-M., Aguilera A., Ansari A.Z., Asturias F.J., Berk A.J., Bjoerklund S., Blackwell T.K., Borggrefe T., Carey M., Carlson M., Conaway J.W., Conaway R.C., Emmons S.W., Fondell J.D., Freedman L.P., Fukasawa T., Gustafsson C.M., Han M. Kornberg R.D. Mol. Cell 14:553-557(2004) [PubMed: 15175151] [Abstract] Cited for: NOMENCLATURE.
[10]	"A high resolution protein interaction map of the yeast Mediator complex." Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M., Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M. Nucleic Acids Res. 32:5379-5391(2004) [PubMed: 15477388] [Abstract] Cited for: STRUCTURE OF THE MEDIATOR COMPLEX.
[11]	"Preponderance of free mediator in the yeast Saccharomyces cerevisiae." Takagi Y., Chadick J.Z., Davis J.A., Asturias F.J. J. Biol. Chem. 280:31200-31207(2005) [PubMed: 16002404] [Abstract] Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX.
[12]	"Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts." Nair D., Kim Y., Myers L.C. J. Biol. Chem. 280:33739-33748(2005) [PubMed: 16076843] [Abstract] Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[13]	"Mediator as a general transcription factor." Takagi Y., Kornberg R.D. J. Biol. Chem. 281:80-89(2006) [PubMed: 16263706] [Abstract] Cited for: FUNCTION OF THE MEDIATOR COMPLEX.
[14]	"Genome-wide location of the coactivator mediator: binding without activation and transient Cdk8 interaction on DNA." Andrau J.-C., van de Pasch L., Lijnzaad P., Bijma T., Koerkamp M.G., van de Peppel J., Werner M., Holstege F.C.P. Mol. Cell 22:179-192(2006) [PubMed: 16630888] [Abstract] Cited for: SUBCELLULAR LOCATION.
[15]	"Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex." Baidoobonso S.M., Guidi B.W., Myers L.C. J. Biol. Chem. 282:5551-5559(2007) [PubMed: 17192271] [Abstract] Cited for: CHARACTERIZATION OF THE MEDIATOR COMPLEX, INTERACTION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[16]	"Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction." Davis J.A., Takagi Y., Kornberg R.D., Asturias F.J. Mol. Cell 10:409-415(2002) [PubMed: 12191485] [Abstract] Cited for: ELECTRON MICROSCOPY OF MEDIATOR COMPLEX IN COMPLEX WITH RNA POLYMERASE II.
[17]	"A conserved mediator hinge revealed in the structure of the MED7-MED21 (Med7-Srb7) heterodimer." Baumli S., Hoeppner S., Cramer P. J. Biol. Chem. 280:18171-18178(2005) [PubMed: 15710619] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 102-205 IN COMPLEX WITH SRB7.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X95465 Genomic DNA. Translation: CAA64734.1.
Z74877 Genomic DNA. Translation: CAA99156.1.
AY692889 Genomic DNA. Translation: AAT92908.1.

PIR

S66832.

RefSeq

NP_014506.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YKE	X-ray	3.30	A/C	102-205	[»]
1YKH	X-ray	3.00	A	102-205	[»]
3FBI	X-ray	2.80	A/C	2-83	[»]
3FBN	X-ray	3.01	A/C	2-83	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1435N.

IntAct

Q08278. 46 interactions.

STRING

Q08278.

Proteomic databases

PeptideAtlas

Q08278.

Genome annotation databases

Ensembl

YOL135C; YOL135C; YOL135C; Saccharomyces cerevisiae. [Genome view]

GeneID

853985.

KEGG

sce:YOL135C.

NMPDR

fig|4932.3.peg.5592.

Organism-specific databases

CYGD

YOL135c.

SGD

S000005495. MED7.

Phylogenomic databases

HOGENOM

Q08278.

OMA

MDSLLLN

OrthoDB

EOG9GQRNT

Gene expression databases

ArrayExpress

Q08278.

Genevestigator

Q08278.

GermOnline

YOL135C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR009244. Mediatior_Med7.
[Graphical view]

PANTHER

PTHR21428. MED7. 1 hit.

Pfam

PF05983. MED7. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

975458.

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Entry information

Entry name

MED7_YEAST

Accession

Primary (citable) accession number: Q08278

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents