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Protein

RING-box protein HRT1

Gene

HRT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes (CRLs), which mediate the ubiquitination of target proteins. Recruits the E2 ubiquitin-conjugating enzyme CDC34/UBC3 to the complex and brings it into close proximity to the substrate. Also stimulates CDC34/UBC3 autoubiquitination and promotes the neddylation of CDC53 and RTT101. Component of the SCF(CDC4) ubiquitin ligase required for ubiquitination of the cyclin-dependent kinase inhibitor SIC1 and for the G1-to-S phase transition. Component of the RTT101(MMS1-MMS22) ubiquitin ligase that promotes fork progression through damaged DNA or natural pause sites. Component of the CRL3(ELA1) ubiquitin ligase required for ubiquitinaton of RPB1, the largest subunit of RNA polymerase II (Pol II), which targets Pol II for proteasomal degradation in DNA-damaged cells.5 Publications

Caution

It is uncertain whether Met-1 or Met-8 is the initiator.Curated

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55Zinc 1By similarity1
Metal bindingi58Zinc 1By similarity1
Metal bindingi66Zinc 2By similarity1
Metal bindingi69Zinc 2By similarity1
Metal bindingi81Zinc 2By similarity1
Metal bindingi88Zinc 3By similarity1
Metal bindingi90Zinc 3; via pros nitrogenBy similarity1
Metal bindingi93Zinc 1; via pros nitrogenBy similarity1
Metal bindingi95Zinc 2By similarity1
Metal bindingi107Zinc 3By similarity1
Metal bindingi110Zinc 3By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri55 – 111RING-typePROSITE-ProRule annotationAdd BLAST57

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • G1/S transition of mitotic cell cycle Source: SGD
  • protein neddylation Source: GO_Central
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

Biological processCell cycle, Cell division, DNA damage, DNA repair, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33528-MONOMER
ReactomeiR-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-5696394 DNA Damage Recognition in GG-NER
R-SCE-5696395 Formation of Incision Complex in GG-NER
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6781823 Formation of TC-NER Pre-Incision Complex
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-68949 Orc1 removal from chromatin
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-8951664 Neddylation
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
RING-box protein HRT1
Short name:
RING-box protein 1
Alternative name(s):
E3 ubiquitin-protein ligase complex SCF subunit HRT1
High level expression reduces Ty3 transposition protein 1
Regulator of cullins protein 1
Gene namesi
Name:HRT1
Synonyms:RBX1, ROC1
Ordered Locus Names:YOL133W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL133W
SGDiS000005493 HRT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi72K → R in RBX1-1; temperature-sensitive allele. At 38 degrees Celsius induces defects in ubiquitin ligase activity; when associated with R-81. 1
Mutagenesisi81C → R in RBX1-1; temperature-sensitive allele. At 38 degrees Celsius induces defects in ubiquitin ligase activity; when associated with R-72. 1
Mutagenesisi81C → Y in HRT1-C81Y; defects in ubiquitin ligase activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000560221 – 121RING-box protein HRT1Add BLAST121

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08273
PaxDbiQ08273
PRIDEiQ08273

PTM databases

iPTMnetiQ08273

Interactioni

Subunit structurei

Component of multiple cullin-RING ligases (CRLs) composed of 4 subunits: the RING protein HRT1, a cullin, a linker protein, and one of many alternative substrate receptors. Component of SCF E3 ubiquitin ligase complexes containing the cullin CDC53, the linker protein SKP1/CBF3D, and substrate receptors containing F-box motifs like DAS1 or GRR1. Component of RTT101(MMS1) E3 ubiquitin ligase complexes containing the cullin RTT101, the linker protein MMS1, and substrate receptors belonging to a protein family described as DCAF (DDB1- and CUL4-associated factor) like MMS22. Component of CRL3 E3 ubiquitin ligase complexes containing the cullin CUL3, the linker protein ELC1, and substrate receptors containing SOCS-box motifs like ELA1. Interacts with CDC53, CUL3, RTT101, CDC4 and CDC34/UBC3.5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • cullin family protein binding Source: GO_Central
  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi34242, 90 interactors
DIPiDIP-1373N
IntActiQ08273, 160 interactors
MINTiQ08273
STRINGi4932.YOL133W

Structurei

3D structure databases

ProteinModelPortaliQ08273
SMRiQ08273
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity.

Sequence similaritiesi

Belongs to the RING-box family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri55 – 111RING-typePROSITE-ProRule annotationAdd BLAST57

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000017058
HOGENOMiHOG000171951
InParanoidiQ08273
KOiK03868
OMAiNHIMEPC
OrthoDBiEOG092C4PUH

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR024766 Znf_RING_H2
PfamiView protein in Pfam
PF12678 zf-rbx1, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q08273-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNEVDRMDV DEDESQNIAQ SSNQSAPVET KKKRFEIKKW TAVAFWSWDI
60 70 80 90 100
AVDNCAICRN HIMEPCIECQ PKAMTDTDNE CVAAWGVCNH AFHLHCINKW
110 120
IKTRDACPLD NQPWQLARCG R
Length:121
Mass (Da):13,940
Last modified:November 1, 1996 - v1
Checksum:iA9C3193E48CAF881
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95465 Genomic DNA Translation: CAA64737.1
Z74876 Genomic DNA Translation: CAA99155.1
BK006948 Genomic DNA Translation: DAA10651.1
PIRiS66830
RefSeqiNP_014508.1, NM_001183387.1

Genome annotation databases

EnsemblFungiiYOL133W; YOL133W; YOL133W
GeneIDi853986
KEGGisce:YOL133W

Similar proteinsi

Entry informationi

Entry nameiRBX1_YEAST
AccessioniPrimary (citable) accession number: Q08273
Secondary accession number(s): D6W1T5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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