Reviewed, UniProtKB/Swiss-Prot Q08269 (ALR1_YEAST)

Names and origin

Protein names

Recommended name:
    Magnesium transporter ALR1
Alternative name(s):
    Aluminum resistance protein 1

Gene names

Name:	ALR1
Ordered Locus Names:	YOL130W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plasma membrane magnesium transporter. Ref.4
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the corA metal ion transporter (MIT) (TC 1.A.35) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	Magnesium
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	magnesium ion transport Inferred from direct assay. Source: SGD transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.4 Inferred from mutant phenotype. Source: SGD
Molecular function	di-, tri-valent inorganic cation transmembrane transporter activity Ref.4 Inferred from mutant phenotype. Source: SGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-2533,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 859	859	Magnesium transporter ALR1		PRO_0000201535
Regions
	Transmembrane	744 – 764	21	Potential
	Transmembrane	773 – 793	21	Potential
Amino acid modifications
	Modified residue	17	1	Phosphoserine Ref.8
	Modified residue	19	1	Phosphoserine Ref.8
	Modified residue	77	1	Phosphotyrosine Ref.6
	Modified residue	184	1	Phosphoserine Ref.8
	Modified residue	188	1	Phosphoserine Ref.5
	Modified residue	220	1	Phosphoserine Ref.6
	Modified residue	221	1	Phosphoserine Ref.6
	Modified residue	232	1	Phosphothreonine Ref.8
	Modified residue	233	1	Phosphoserine Ref.8 Ref.7
	Modified residue	236	1	Phosphoserine Ref.8 Ref.6 Ref.7
	Modified residue	242	1	Phosphothreonine Ref.6 Ref.7
	Modified residue	357	1	Phosphoserine Ref.8
	Modified residue	850	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	13	1	N → Y in AAC49462. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q08269-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6DA44CA70EFA2693	FASTA	859	95,869
	10 20 30 40 50 60 MSSSSSSSES SPNLSRSNSL ANTMVSMKTE DHTGLYDHRQ HPDSLPVRHQ PPTLKNKEIA 70 80 90 100 110 120 KSTKPSIPKE QKSATRYNSH VDVGSVPSRG RMDFEDEGQG MDETVAHHQL RASAILTSNA 130 140 150 160 170 180 RPSRLAHSMP HQRQLYVESN IHTPPKDVGV KRDYTMSSST ASSGNKSKLS ASSSASPITK 190 200 210 220 230 240 VRKSSLVSPV LEIPHESKSD THSKLAKPKK RTYSTTSAHS SINPAVLLTK STSQKSDADD 250 260 270 280 290 300 DTLERKPVRM NTRASFDSDV SQASRDSQET EEDVCFPMPP QLHTRVNGID FDELEEYAQF 310 320 330 340 350 360 ANAEKSQFLA SLQVPNEQKY SNVSQDIGFT SSTSTSGSSA ALKYTPRVSQ TGEKSESTNE 370 380 390 400 410 420 TEIHEKKEDE HEKIKPSLHP GISFGKNKVE GEENENIPSN DPAYCSYQGT DFQIPNRFSF 430 440 450 460 470 480 FCSESDETVH ASDIPSLVSE GQTFYELFRG GEPTWWLDCS CPTDDEMRCI AKAFGIHPLT 490 500 510 520 530 540 AEDIRMQETR EKVELFKSYY FVCFHTFEND KESEDFLEPI NVYIVVCRSG VLTFHFGPIS 550 560 570 580 590 600 HCANVRRRVR QLRDYVNVNS DWLCYALIDD ITDSFAPVIQ SIEYEADAIE DSVFMARDMD 610 620 630 640 650 660 FAAMLQRIGE SRRKTMTLMR LLSGKADVIK MFAKRCQDEA NGIGPALTSQ INIANLQARQ 670 680 690 700 710 720 DNASHIKNNS STTVPNNYAP TTSQPRGDIA LYLGDIQDHL LTMFQNLLAY EKIFSRSHTN 730 740 750 760 770 780 YLAQLQVESF NSNNKVTEML GKVTMIGTML VPLNVITGLF GMNVKVPGEN SSIAWWFGIL 790 800 810 820 830 840 GVLLLLAVLG WFLASYWIKR IDPPATLNEA AESGAKSVIS SFLPKRNKRF NDRSKNINVR 850 AGPSNKSVAS LPSRYSRYD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 13.4 kbp fragment from the left arm of chromosome XV reveals a malate dehydrogenase gene, a putative Ser/Thr protein kinase, the ribosomal L25 gene and four new open reading frames." Casamayor A., Khalid H., Balcells L., Aldea M., Casas C., Herrero E., Arino J. Yeast 12:1013-1020(1996) [PubMed: 8896265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Overexpression of the Saccharomyces cerevisiae magnesium transport system confers resistance to aluminum ion." MacDiarmid C.W., Gardner R.C. J. Biol. Chem. 273:1727-1732(1998) [PubMed: 9430719] [Abstract] Cited for: FUNCTION.
[5]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188, MASS SPECTROMETRY.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-77; SER-220; SER-221; SER-236 AND THR-242, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-233; SER-236 AND THR-242, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-19; SER-184; THR-232; SER-233; SER-236; SER-357 AND SER-850, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U41293 Genomic DNA. Translation: AAC49462.1. Z74872 Genomic DNA. Translation: CAA99150.1. Z74871 Genomic DNA. Translation: CAA99149.1. AY692762 Genomic DNA. Translation: AAT92781.1.
PIR	S66827.
RefSeq	NP_014511.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1184N.
IntAct	Q08269. 12 interactions.
STRING	Q08269.
Protein family/group databases
TCDB	1.A.35.2.1. CorA metal ion transporter (MIT) family.
Proteomic databases
PeptideAtlas	Q08269.
Genome annotation databases
Ensembl	YOL130W; YOL130W; YOL130W; Saccharomyces cerevisiae. [Genome view]
GeneID	853990.
KEGG	sce:YOL130W.
NMPDR	fig|4932.3.peg.5597.
Organism-specific databases
CYGD	YOL130w.
SGD	S000005490. ALR1.
Phylogenomic databases
HOGENOM	Q08269.
OMA	GKVTMIG
OrthoDB	EOG9D2863
Gene expression databases
ArrayExpress	Q08269.
Genevestigator	Q08269.
GermOnline	YOL130W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002523. Mg2+_transptCorA. [Graphical view]
PANTHER	PTHR21535:SF1. Mg2+_transptCorA. 1 hit.
Pfam	PF01544. CorA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	975470.

Entry information

Entry name

ALR1_YEAST

Accession

Primary (citable) accession number: Q08269
Secondary accession number(s): Q02811

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)