ID   PCKG_CHLLI              Reviewed;         618 AA.
AC   Q08262;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2002, sequence version 2.
DT   16-JUN-2009, entry version 61.
DE   RecName: Full=Phosphoenolpyruvate carboxykinase [GTP];
DE            Short=PEP carboxykinase;
DE            Short=PEPCK;
DE            EC=4.1.1.32;
DE   AltName: Full=Phosphoenolpyruvate carboxylase;
GN   Name=pckG; Synonyms=pckA;
OS   Chlorobium limicola.
OC   Bacteria; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae;
OC   Chlorobium/Pelodictyon group; Chlorobium.
OX   NCBI_TaxID=1092;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=93192317; PubMed=8448205; DOI=10.1016/0167-4781(93)90213-W;
RA   Xie D.L., Lill H., Hauska G., Maeda M., Futai M., Nelson N.;
RT   "The atp2 operon of the green bacterium Chlorobium limicola.";
RL   Biochim. Biophys. Acta 1172:267-273(1993).
CC   -!- FUNCTION: Catalyzes the conversion of oxaloacetate (OAA) to
CC       phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic
CC       pathway that produces glucose from lactate and other precursors
CC       derived from the citric acid cycle (By similarity).
CC   -!- CATALYTIC ACTIVITY: GTP + oxaloacetate = GDP + phosphoenolpyruvate
CC       + CO(2).
CC   -!- COFACTOR: Binds 1 manganese ion per subunit (By similarity).
CC   -!- PATHWAY: Carbohydrate biosynthesis; gluconeogenesis.
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase [GTP]
CC       family.
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DR   EMBL; S56812; AAB25776.1; ALT_INIT; Genomic_DNA.
DR   HSSP; P35558; 1KHB.
DR   BRENDA; 4.1.1.32; 13.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:HAMAP.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) act...; IEA:HAMAP.
DR   GO; GO:0006094; P:gluconeogenesis; IEA:HAMAP.
DR   HAMAP; MF_00452; -; 1.
DR   InterPro; IPR018091; PEP_carboxykin_GTP_CS.
DR   InterPro; IPR013035; PEP_carboxykinase_C.
DR   InterPro; IPR008209; PEP_carboxykinase_GTP.
DR   InterPro; IPR008210; PEP_carboxykinase_N.
DR   Gene3D; G3DSA:3.90.228.20; PEP_carboxykinase_C; 1.
DR   Gene3D; G3DSA:3.40.449.10; PEP_carboxykinase_N; 1.
DR   PANTHER; PTHR11561; PEP_carboxykin; 1.
DR   Pfam; PF00821; PEPCK; 1.
DR   PIRSF; PIRSF001348; PEP_carboxykinase_GTP; 1.
DR   ProDom; PD004738; PEPCK_N; 1.
DR   PROSITE; PS00505; PEPCK_GTP; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Decarboxylase; Gluconeogenesis; GTP-binding; Lyase;
KW   Manganese; Metal-binding; Nucleotide-binding.
FT   CHAIN         1    618       Phosphoenolpyruvate carboxykinase [GTP].
FT                                /FTId=PRO_0000103595.
FT   NP_BIND     270    275       GTP (By similarity).
FT   NP_BIND     510    513       GTP (By similarity).
FT   REGION      386    388       Substrate binding (By similarity).
FT   ACT_SITE    271    271       By similarity.
FT   METAL       227    227       Manganese (By similarity).
FT   METAL       247    247       Manganese (By similarity).
FT   METAL       296    296       Manganese (By similarity).
FT   BINDING      79     79       Substrate (By similarity).
FT   BINDING     220    220       Substrate; via amide nitrogen (By
FT                                similarity).
FT   BINDING     227    227       Substrate (By similarity).
FT   BINDING     269    269       Substrate (By similarity).
FT   BINDING     388    388       GTP (By similarity).
FT   BINDING     419    419       GTP (By similarity).
SQ   SEQUENCE   618 AA;  69531 MW;  274A1FA8D0612C29 CRC64;
     MEPIPINAPD YVKNLKLLQW VKETVALCQP DSVCWCDGST EEYDRLCEEM VNSGTFIKLS
     EQKRPNSYLC RSDPSDVARV EDRTFICSIR RQDAGPTNNW VAPKEMKATL NKLFTGCMKG
     RTMYVIPFSM GPLGSHIAHI GVEITDSPYV VTNMRIMTRM GRQVLELLDE EAEFVPCLHS
     VGAPLEPGQA DVPWPCNDTK YIVHFPEEHA IVSYSSGYNG NTLLGKKCFA LRIASSMARD
     EGWLAEHMLI LGVESPAGEK DYVAAAFPSA CGKTNFAMII PPGEMEGWKI TTVGDDIRLD
     QQGKDGRLHA INPEYGFFGV APGTSDKSNP NAMATLHANC IFTNVALTPD GDVWWEDMTD
     TPPEYLIDWQ GKPWVPGCDR PAAHPNSRFT APASQCPVID PAWENPRVLP ISAFIFAGRR
     GDTIPLVYQS SNWYYGVYMA ATMGSEKTAA AARSATCVDP FAMLPFCGYH MGDYFNHWLH
     VGRTVSDPPR IFIVNWFRKT RTGKFLWPGF GDNMRVLKWM MGRVHGRSGA VESPLGWMPR
     YESLDWRGLD GFTSNKFSKL MSVDREGKQE LFSHEELLEK LYDRLPKEFT HIRELLLSTL
     WMSPEHWELA PELYSAES
//