Reviewed,
UniProtKB/Swiss-Prot Q08262 (PCKG_CHLLI)
Last modified
June 16, 2009.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Phosphoenolpyruvate carboxykinase [GTP] Short name=PEP carboxykinase Short name=PEPCK EC=4.1.1.32 Alternative name(s): Phosphoenolpyruvate carboxylase | ||||
| Gene names |
| ||||
| Organism | Chlorobium limicola | ||||
| Taxonomic identifier | 1092 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium |
Protein attributes
| Sequence length | 618 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. |
| Catalytic activity | GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP MF_00452 |
| Cofactor | Binds 1 manganese ion per subunit By similarity. |
| Pathway | |
| Subunit structure | Monomer By similarity. |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Gluconeogenesis |
| Cellular component | Cytoplasm |
| Ligand | GTP-binding Manganese Metal-binding Nucleotide-binding |
| Molecular function | Decarboxylase Lyase |
| Gene Ontology (GO) | |
| Biological process | gluconeogenesis Inferred from electronic annotation. Source: HAMAP |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | GTP binding Inferred from electronic annotation. Source: HAMAP manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate carboxykinase (GTP) activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 618 | 618 | Phosphoenolpyruvate carboxykinase [GTP] HAMAP MF_00452 | PRO_0000103595 | |||||
Regions | |||||||||
| Nucleotide binding | 270 – 275 | 6 | GTP By similarity | ||||||
| Nucleotide binding | 510 – 513 | 4 | GTP By similarity | ||||||
| Region | 386 – 388 | 3 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 271 | 1 | By similarity | ||||||
| Metal binding | 227 | 1 | Manganese By similarity | ||||||
| Metal binding | 247 | 1 | Manganese By similarity | ||||||
| Metal binding | 296 | 1 | Manganese By similarity | ||||||
| Binding site | 79 | 1 | Substrate By similarity | ||||||
| Binding site | 220 | 1 | Substrate; via amide nitrogen By similarity | ||||||
| Binding site | 227 | 1 | Substrate By similarity | ||||||
| Binding site | 269 | 1 | Substrate By similarity | ||||||
| Binding site | 388 | 1 | GTP By similarity | ||||||
| Binding site | 419 | 1 | GTP By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "The atp2 operon of the green bacterium Chlorobium limicola." Xie D.L., Lill H., Hauska G., Maeda M., Futai M., Nelson N. Biochim. Biophys. Acta 1172:267-273(1993) [PubMed: 8448205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| S56812 Genomic DNA. Translation: AAB25776.1. Different initiation. | |
3D structure databases | |
| HSSP | HSSP built from PDB template 1KHB based on UniProtKB P35558. |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 4.1.1.32. 13. |
Family and domain databases | |
| HAMAP | MF_00452. [Tree] |
| InterPro | IPR018091. PEP_carboxykin_GTP_CS. IPR013035. PEP_carboxykinase_C. IPR008209. PEP_carboxykinase_GTP. IPR008210. PEP_carboxykinase_N. [Graphical view] |
| Gene3D | G3DSA:3.90.228.20. PEP_carboxykinase_C. 1 hit. G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit. |
| PANTHER | PTHR11561. PEP_carboxykin. 1 hit. |
| Pfam | PF00821. PEPCK. 1 hit. [Graphical view] |
| PIRSF | PIRSF001348. PEP_carboxykinase_GTP. 1 hit. |
| ProDom | PD004738. PEPCK_N. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00505. PEPCK_GTP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PCKG_CHLLI | ||||||||
| Accession | Primary (citable) accession number: Q08262 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


