Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q08262

- PCKG_CHLLI

UniProt

Q08262 - PCKG_CHLLI

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Chlorobium limicola
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (25 Nov 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei79 – 791SubstrateUniRule annotation
    Binding sitei220 – 2201Substrate; via amide nitrogenUniRule annotation
    Metal bindingi227 – 2271ManganeseUniRule annotation
    Binding sitei227 – 2271SubstrateUniRule annotation
    Metal bindingi247 – 2471Manganese; via tele nitrogenUniRule annotation
    Binding sitei269 – 2691SubstrateUniRule annotation
    Active sitei271 – 2711UniRule annotation
    Metal bindingi296 – 2961ManganeseUniRule annotation
    Binding sitei388 – 3881GTPUniRule annotation
    Binding sitei419 – 4191GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi270 – 2756GTPUniRule annotation
    Nucleotide bindingi510 – 5134GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Synonyms:pckA
    OrganismiChlorobium limicola
    Taxonomic identifieri1092 [NCBI]
    Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 618618Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103595Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ08262.
    SMRiQ08262. Positions 11-599.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni386 – 3883Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08262-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPIPINAPD YVKNLKLLQW VKETVALCQP DSVCWCDGST EEYDRLCEEM    50
    VNSGTFIKLS EQKRPNSYLC RSDPSDVARV EDRTFICSIR RQDAGPTNNW 100
    VAPKEMKATL NKLFTGCMKG RTMYVIPFSM GPLGSHIAHI GVEITDSPYV 150
    VTNMRIMTRM GRQVLELLDE EAEFVPCLHS VGAPLEPGQA DVPWPCNDTK 200
    YIVHFPEEHA IVSYSSGYNG NTLLGKKCFA LRIASSMARD EGWLAEHMLI 250
    LGVESPAGEK DYVAAAFPSA CGKTNFAMII PPGEMEGWKI TTVGDDIRLD 300
    QQGKDGRLHA INPEYGFFGV APGTSDKSNP NAMATLHANC IFTNVALTPD 350
    GDVWWEDMTD TPPEYLIDWQ GKPWVPGCDR PAAHPNSRFT APASQCPVID 400
    PAWENPRVLP ISAFIFAGRR GDTIPLVYQS SNWYYGVYMA ATMGSEKTAA 450
    AARSATCVDP FAMLPFCGYH MGDYFNHWLH VGRTVSDPPR IFIVNWFRKT 500
    RTGKFLWPGF GDNMRVLKWM MGRVHGRSGA VESPLGWMPR YESLDWRGLD 550
    GFTSNKFSKL MSVDREGKQE LFSHEELLEK LYDRLPKEFT HIRELLLSTL 600
    WMSPEHWELA PELYSAES 618
    Length:618
    Mass (Da):69,531
    Last modified:November 25, 2002 - v2
    Checksum:i274A1FA8D0612C29
    GO

    Sequence cautioni

    The sequence AAB25776.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S56812 Genomic DNA. Translation: AAB25776.1. Different initiation.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S56812 Genomic DNA. Translation: AAB25776.1 . Different initiation.

    3D structure databases

    ProteinModelPortali Q08262.
    SMRi Q08262. Positions 11-599.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00138 .

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The atp2 operon of the green bacterium Chlorobium limicola."
      Xie D.L., Lill H., Hauska G., Maeda M., Futai M., Nelson N.
      Biochim. Biophys. Acta 1172:267-273(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiPCKG_CHLLI
    AccessioniPrimary (citable) accession number: Q08262
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: November 25, 2002
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3