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Q08262

- PCKG_CHLLI

UniProt

Q08262 - PCKG_CHLLI

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Chlorobium limicola
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791SubstrateUniRule annotation
Binding sitei220 – 2201Substrate; via amide nitrogenUniRule annotation
Metal bindingi227 – 2271ManganeseUniRule annotation
Binding sitei227 – 2271SubstrateUniRule annotation
Metal bindingi247 – 2471Manganese; via tele nitrogenUniRule annotation
Binding sitei269 – 2691SubstrateUniRule annotation
Active sitei271 – 2711UniRule annotation
Metal bindingi296 – 2961ManganeseUniRule annotation
Binding sitei388 – 3881GTPUniRule annotation
Binding sitei419 – 4191GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi270 – 2756GTPUniRule annotation
Nucleotide bindingi510 – 5134GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pckA
OrganismiChlorobium limicola
Taxonomic identifieri1092 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 618618Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103595Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ08262.
SMRiQ08262. Positions 11-599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni386 – 3883Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08262 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEPIPINAPD YVKNLKLLQW VKETVALCQP DSVCWCDGST EEYDRLCEEM
60 70 80 90 100
VNSGTFIKLS EQKRPNSYLC RSDPSDVARV EDRTFICSIR RQDAGPTNNW
110 120 130 140 150
VAPKEMKATL NKLFTGCMKG RTMYVIPFSM GPLGSHIAHI GVEITDSPYV
160 170 180 190 200
VTNMRIMTRM GRQVLELLDE EAEFVPCLHS VGAPLEPGQA DVPWPCNDTK
210 220 230 240 250
YIVHFPEEHA IVSYSSGYNG NTLLGKKCFA LRIASSMARD EGWLAEHMLI
260 270 280 290 300
LGVESPAGEK DYVAAAFPSA CGKTNFAMII PPGEMEGWKI TTVGDDIRLD
310 320 330 340 350
QQGKDGRLHA INPEYGFFGV APGTSDKSNP NAMATLHANC IFTNVALTPD
360 370 380 390 400
GDVWWEDMTD TPPEYLIDWQ GKPWVPGCDR PAAHPNSRFT APASQCPVID
410 420 430 440 450
PAWENPRVLP ISAFIFAGRR GDTIPLVYQS SNWYYGVYMA ATMGSEKTAA
460 470 480 490 500
AARSATCVDP FAMLPFCGYH MGDYFNHWLH VGRTVSDPPR IFIVNWFRKT
510 520 530 540 550
RTGKFLWPGF GDNMRVLKWM MGRVHGRSGA VESPLGWMPR YESLDWRGLD
560 570 580 590 600
GFTSNKFSKL MSVDREGKQE LFSHEELLEK LYDRLPKEFT HIRELLLSTL
610
WMSPEHWELA PELYSAES
Length:618
Mass (Da):69,531
Last modified:November 25, 2002 - v2
Checksum:i274A1FA8D0612C29
GO

Sequence cautioni

The sequence AAB25776.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S56812 Genomic DNA. Translation: AAB25776.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S56812 Genomic DNA. Translation: AAB25776.1 . Different initiation.

3D structure databases

ProteinModelPortali Q08262.
SMRi Q08262. Positions 11-599.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00138 .

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The atp2 operon of the green bacterium Chlorobium limicola."
    Xie D.L., Lill H., Hauska G., Maeda M., Futai M., Nelson N.
    Biochim. Biophys. Acta 1172:267-273(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPCKG_CHLLI
AccessioniPrimary (citable) accession number: Q08262
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 25, 2002
Last modified: October 29, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3