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Q08262

- PCKG_CHLLI

UniProt

Q08262 - PCKG_CHLLI

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene
pckG, pckA
Organism
Chlorobium limicola
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791Substrate By similarity
Binding sitei220 – 2201Substrate; via amide nitrogen By similarity
Metal bindingi227 – 2271Manganese By similarity
Binding sitei227 – 2271Substrate By similarity
Metal bindingi247 – 2471Manganese; via tele nitrogen By similarity
Binding sitei269 – 2691Substrate By similarity
Active sitei271 – 2711 By similarity
Metal bindingi296 – 2961Manganese By similarity
Binding sitei388 – 3881GTP By similarity
Binding sitei419 – 4191GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi270 – 2756GTP By similarity
Nucleotide bindingi510 – 5134GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Synonyms:pckA
OrganismiChlorobium limicola
Taxonomic identifieri1092 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 618618Phosphoenolpyruvate carboxykinase [GTP]UniRule annotationPRO_0000103595Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ08262.
SMRiQ08262. Positions 11-599.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni386 – 3883Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08262-1 [UniParc]FASTAAdd to Basket

« Hide

MEPIPINAPD YVKNLKLLQW VKETVALCQP DSVCWCDGST EEYDRLCEEM    50
VNSGTFIKLS EQKRPNSYLC RSDPSDVARV EDRTFICSIR RQDAGPTNNW 100
VAPKEMKATL NKLFTGCMKG RTMYVIPFSM GPLGSHIAHI GVEITDSPYV 150
VTNMRIMTRM GRQVLELLDE EAEFVPCLHS VGAPLEPGQA DVPWPCNDTK 200
YIVHFPEEHA IVSYSSGYNG NTLLGKKCFA LRIASSMARD EGWLAEHMLI 250
LGVESPAGEK DYVAAAFPSA CGKTNFAMII PPGEMEGWKI TTVGDDIRLD 300
QQGKDGRLHA INPEYGFFGV APGTSDKSNP NAMATLHANC IFTNVALTPD 350
GDVWWEDMTD TPPEYLIDWQ GKPWVPGCDR PAAHPNSRFT APASQCPVID 400
PAWENPRVLP ISAFIFAGRR GDTIPLVYQS SNWYYGVYMA ATMGSEKTAA 450
AARSATCVDP FAMLPFCGYH MGDYFNHWLH VGRTVSDPPR IFIVNWFRKT 500
RTGKFLWPGF GDNMRVLKWM MGRVHGRSGA VESPLGWMPR YESLDWRGLD 550
GFTSNKFSKL MSVDREGKQE LFSHEELLEK LYDRLPKEFT HIRELLLSTL 600
WMSPEHWELA PELYSAES 618
Length:618
Mass (Da):69,531
Last modified:November 25, 2002 - v2
Checksum:i274A1FA8D0612C29
GO

Sequence cautioni

The sequence AAB25776.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S56812 Genomic DNA. Translation: AAB25776.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S56812 Genomic DNA. Translation: AAB25776.1 . Different initiation.

3D structure databases

ProteinModelPortali Q08262.
SMRi Q08262. Positions 11-599.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00138 .

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The atp2 operon of the green bacterium Chlorobium limicola."
    Xie D.L., Lill H., Hauska G., Maeda M., Futai M., Nelson N.
    Biochim. Biophys. Acta 1172:267-273(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPCKG_CHLLI
AccessioniPrimary (citable) accession number: Q08262
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 25, 2002
Last modified: April 16, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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