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Q08258 (GLTB_HORVU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ferredoxin-dependent glutamate synthase

EC=1.4.7.1
Alternative name(s):
FD-GOGAT
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Protein attributes

Sequence length436 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+.

Cofactor

Binds 1 3Fe-4S cluster.

FAD.

FMN.

Pathway

Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1.

Energy metabolism; nitrogen metabolism.

Subunit structure

Monomer.

Subcellular location

Plastidchloroplast stroma.

Sequence similarities

Belongs to the glutamate synthase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›436›436Ferredoxin-dependent glutamate synthase
PRO_0000170795

Regions

Domain1 – 400400Glutamine amidotransferase type-2

Sites

Active site11For GATase activity By similarity

Experimental info

Non-terminal residue4361

Sequences

Sequence LengthMass (Da)Tools
Q08258 [UniParc].

Last modified December 12, 2006. Version 2.
Checksum: 63E0C7CCD04400B6

FASTA43648,752
        10         20         30         40         50         60 
CGVGFVANLS NEPSFNVVRD ALTALGCMEH RGGCGADNDS GDGAGLMSGI PWDLFDDWAS 

        70         80         90        100        110        120 
KEGLVPFERT HTGVGMVFLP QNENSMEEAK AAVEKVFTDE GLEVLGWRPV PFNLSVAGRN 

       130        140        150        160        170        180 
AKETMPNILQ IFVRIAKEDD ADDIERELYI CRKLIERATK SASWADELYF CSLSSRTIIY 

       190        200        210        220        230        240 
KGMLRSEVLG QFYLDLQNEL YKSPFAIYHR RFSTNTSPRW PLAQPMRLLG HNGEINTIQG 

       250        260        270        280        290        300 
NLNWMRSREA TIQSPVWRGR ENELRPFGDP KASDSANLDN AAELLLRSGR SPAEAMMMLV 

       310        320        330        340        350        360 
PEAYKNHPTL SVKYPEVIDF YEYYKGQMEA WDGPALLLFS DGRTVGACLD RNGLRPARYW 

       370        380        390        400        410        420 
KTSDGFVYVA SEVGVIPMDE SKVVMKGRLG PGMMITVDLE TGQVLENTEV KKNVASAKPY 

       430 
GTWLQESTRS IKPVNF 

« Hide

References

[1]"Cloning and sequence analysis of a cDNA for barley ferredoxin-dependent glutamate synthase and molecular analysis of photorespiratory mutants deficient in the enzyme."
Avila C., Marquez A.J., Pajuelo P., Cannell M.E., Wallsgrove R.M., Forde B.G.
Planta 189:475-483(1993) [PubMed: 7763576] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-436, PARTIAL PROTEIN SEQUENCE.
Strain: cv. Maris Mink.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S58774 mRNA. Translation: AAC60547.1.
PIRT06210.

3D structure databases

ProteinModelPortalQ08258.
SMRQ08258. Positions 1-428.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ08258.

Gene expression databases

GenevestigatorQ08258.

Family and domain databases

InterProIPR000583. GATase_2.
IPR017932. GATase_II.
[Graphical view]
PfamPF00310. GATase_2. 1 hit.
[Graphical view]
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLTB_HORVU
AccessionPrimary (citable) accession number: Q08258
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 12, 2006
Last modified: October 19, 2011
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families