Reviewed, UniProtKB/Swiss-Prot Q08258 (GLTB_HORVU)

Names and origin

Protein names	Recommended name:     Ferredoxin-dependent glutamate synthase     EC=1.4.7.1 Alternative name(s):     FD-GOGAT
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	436 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+.
Cofactor	Binds 1 3Fe-4S cluster. FAD. FMN.
Pathway	Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1. Energy metabolism; nitrogen metabolism.
Subunit structure	Monomer.
Subcellular location	Plastid › chloroplast stroma.
Sequence similarities	Belongs to the glutamate synthase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Glutamate biosynthesis
Cellular component	Chloroplast Plastid
Domain	Glutamine amidotransferase
Ligand	3Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate synthase (ferredoxin) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›436	›436	Ferredoxin-dependent glutamate synthase		PRO_0000170795
Regions
	Domain	1 – 400	400	Glutamine amidotransferase type-2
Sites
	Active site	1	1	For GATase activity By similarity
Experimental info
	Non-terminal residue	436	1

Sequences

	Sequence		Length	Mass (Da)
	Q08258-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: 63E0C7CCD04400B6	FASTA	436	48,752
	10 20 30 40 50 60 CGVGFVANLS NEPSFNVVRD ALTALGCMEH RGGCGADNDS GDGAGLMSGI PWDLFDDWAS 70 80 90 100 110 120 KEGLVPFERT HTGVGMVFLP QNENSMEEAK AAVEKVFTDE GLEVLGWRPV PFNLSVAGRN 130 140 150 160 170 180 AKETMPNILQ IFVRIAKEDD ADDIERELYI CRKLIERATK SASWADELYF CSLSSRTIIY 190 200 210 220 230 240 KGMLRSEVLG QFYLDLQNEL YKSPFAIYHR RFSTNTSPRW PLAQPMRLLG HNGEINTIQG 250 260 270 280 290 300 NLNWMRSREA TIQSPVWRGR ENELRPFGDP KASDSANLDN AAELLLRSGR SPAEAMMMLV 310 320 330 340 350 360 PEAYKNHPTL SVKYPEVIDF YEYYKGQMEA WDGPALLLFS DGRTVGACLD RNGLRPARYW 370 380 390 400 410 420 KTSDGFVYVA SEVGVIPMDE SKVVMKGRLG PGMMITVDLE TGQVLENTEV KKNVASAKPY 430 GTWLQESTRS IKPVNF

References

[1]

"Cloning and sequence analysis of a cDNA for barley ferredoxin-dependent glutamate synthase and molecular analysis of photorespiratory mutants deficient in the enzyme." Avila C., Marquez A.J., Pajuelo P., Cannell M.E., Wallsgrove R.M., Forde B.G. Planta 189:475-483(1993) [PubMed: 7763576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-436, PARTIAL PROTEIN SEQUENCE. Strain: cv. Maris Mink.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S58774 mRNA. Translation: AAC60547.1.
PIR	T06210.
UniGene	Hv.25558
3D structure databases
HSSP	HSSP built from PDB template 1LM1 based on UniProtKB P55038.
ModBase	Search...
Organism-specific databases
Gramene	Q08258.
Enzyme and pathway databases
BRENDA	1.4.7.1. 283.
Family and domain databases
InterPro	IPR000583. GATase_2. IPR017932. GATase_II. [Graphical view]
Pfam	PF00310. GATase_2. 1 hit. [Graphical view]
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLTB_HORVU

Accession

Primary (citable) accession number: Q08258

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)