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Protein

Target of rapamycin complex 2 subunit AVO1

Gene

AVO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of TORC2, which regulates cell cycle-dependent polarization of the actin-cytoskeleton and cell wall integrity. TORC2 controls polarity of the actin cytoskeleton, which is required for orienting the secretory pathway toward discrete growth sites, via the RHO1/PKC1/MAPK cell integrity pathway.

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • phosphatidylinositol-4,5-bisphosphate binding Source: SGD
  • Ras GTPase binding Source: SGD

GO - Biological processi

  • establishment or maintenance of actin cytoskeleton polarity Source: SGD
  • fungal-type cell wall organization Source: SGD
  • negative regulation of Ras protein signal transduction Source: SGD
  • regulation of cell growth Source: SGD
  • stress-activated protein kinase signaling cascade Source: GO_Central
  • TORC2 signaling Source: GO_Central
  • TOR signaling Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-33482-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Target of rapamycin complex 2 subunit AVO1
Short name:
TORC2 subunit AVO1
Alternative name(s):
Adheres voraciously to TOR2 protein 1
Gene namesi
Name:AVO1
Ordered Locus Names:YOL078W
ORF Names:O1110
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL078W
SGDiS000005438 AVO1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187711 – 1176Target of rapamycin complex 2 subunit AVO1Add BLAST1176

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei140PhosphoserineCombined sources1
Modified residuei144PhosphoserineCombined sources1
Modified residuei400PhosphoserineCombined sources1
Modified residuei509PhosphoserineCombined sources1
Modified residuei510PhosphothreonineCombined sources1
Modified residuei552PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated by TORC2.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ08236
PaxDbiQ08236
PRIDEiQ08236

PTM databases

CarbonylDBiQ08236
iPTMnetiQ08236

Interactioni

Subunit structurei

The target of rapamycin complex 2 (TORC2) is composed of at least AVO1, AVO2, BIT61, LST8, TOR2 and TSC11. TORC2 likely forms a homodimer. Contrary to TORC1, TORC2 does not bind to and is not sensitive to FKBP-rapamycin. AVO1 is required for TORC2 integrity by tethering AVO2 and TSC11 to the complex.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • Ras GTPase binding Source: SGD

Protein-protein interaction databases

BioGridi34323, 429 interactors
DIPiDIP-6357N
IntActiQ08236, 16 interactors
MINTiQ08236
STRINGi4932.YOL078W

Structurei

Secondary structure

11176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1069 – 1090Combined sources22
Beta strandi1093 – 1097Combined sources5
Beta strandi1112 – 1115Combined sources4
Helixi1116 – 1118Combined sources3
Beta strandi1119 – 1124Combined sources6
Beta strandi1131 – 1136Combined sources6
Beta strandi1145 – 1153Combined sources9
Helixi1154 – 1173Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ULBX-ray1.90A/B1056-1176[»]
3ULCX-ray2.80A1056-1176[»]
6EMKelectron microscopy8.00I/J1-1176[»]
ProteinModelPortaliQ08236
SMRiQ08236
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SIN1 family.Curated

Phylogenomic databases

HOGENOMiHOG000034238
InParanoidiQ08236
OMAiRYYFEAV
OrthoDBiEOG092C0HRG

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR031567 CRIM_dom
IPR011993 PH-like_dom_sf
IPR031313 Sin1_PH_dom
PfamiView protein in Pfam
PF16978 CRIM, 1 hit
PF16979 SIN1_PH, 1 hit

Sequencei

Sequence statusi: Complete.

Q08236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTVTVLNEL RAQFLRVCPE KDQMKRIIKP YIPVDEFNTE QCLDSSIREL
60 70 80 90 100
YMNSDGVSLL PELESPPVSK DFMENYASLG KMRIMRENEG QKGKANQNLI
110 120 130 140 150
GAEKTERDEE ETRNLQDKSA KNTLIVEENG TLRYNPLNSS ASNSLLNDDD
160 170 180 190 200
HTSGKHHKTS SKEDSYLNSS MEMQKKSSKR SSLPFVRIFK SRRDHSNTSG
210 220 230 240 250
NKNVMNTTNT RAKSSTLHPP GARHNKKGSK FDMNFDFDEN LEEEDDDDDD
260 270 280 290 300
DEEGDDIHSQ FFQLDDDFDA KGSGASPAHK GINGMSNNKN NTYTNNRNSI
310 320 330 340 350
SILDDRESSN GNIGSASRLK SHFPTSQKGK IFLTDNKNDG QKSDSLNANK
360 370 380 390 400
GIHGDGSSAS GNGSVSRDGL TETESNNISD MESYINEKDL DDLNFDTVTS
410 420 430 440 450
NINKTVSDLG GHESTNDGTA VMNRDSKDSR SNSNEFNAQN RDRITPGSSY
460 470 480 490 500
GKSLLGSEYS EERYSNNDSS TMESGEMSLD SDMQTNTIPS HSIPMSMQKY
510 520 530 540 550
GIYHGDDDST LNNVFDKAVL TMNSSRHPKE RRDTVISGKE PTSLTSSNRK
560 570 580 590 600
FSVSSNLTST RSPLLRGHGR TSSTASSEHM KAPKVSDSVL HRARKSTLTL
610 620 630 640 650
KQDHSQPSVP SSVHKSSKEG NILIEKTTDY LVSKPKASQL SNMFNKKKKR
660 670 680 690 700
TNTNSVDVLE YFSFVCGDKV PNYESMGLEI YIQASKKYKR NSFTTKVRKS
710 720 730 740 750
STIFEVIGFA LFLYSTEKKP DNFEEDGLTV EDISNPNNFS LKIVDEDGEP
760 770 780 790 800
FEDNFGKLDR KSTIQSISDS EVVLCKVDDA EKSQNEIETP LPFETGGGLM
810 820 830 840 850
DASTLDANSS HDTTDGTINQ LSFYKPIIGN EDDIDKTNGS KIIDVTVYLY
860 870 880 890 900
PNVNPKFNYT TISVLVTSHI NDILVKYCKM KNMDPNEYAL KVLGKNYILD
910 920 930 940 950
LNDTVLRLDG INKVELISKK DARELHLEKM KPDLKKPVLP TIQSNDLTPL
960 970 980 990 1000
TLEPLNSYLK ADAGGAVAAI PENTKVTSKA KKISTKYKLG LAKQHSSSSV
1010 1020 1030 1040 1050
ASGSVSTAGG LANGNGFFKN KNSSKSSLHG TLQFHNINRS QSTMEHTPDT
1060 1070 1080 1090 1100
PNGVGDNFQD LFTGAYHKYK VWRRQQMSFI NKHERTLAID GDYIYIVPPE
1110 1120 1130 1140 1150
GRIHWHDNVK TKSLHISQVV LVKKSKRVPE HFKIFVRREG QDDIKRYYFE
1160 1170
AVSGQECTEI VTRLQNLLSA YRMNHK
Length:1,176
Mass (Da):131,379
Last modified:November 1, 1996 - v1
Checksum:iED2B30D014EE5652
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74820 Genomic DNA Translation: CAA99089.1
Z74819 Genomic DNA Translation: CAA99088.1
BK006948 Genomic DNA Translation: DAA10705.1
PIRiS66771
RefSeqiNP_014563.1, NM_001183332.1

Genome annotation databases

EnsemblFungiiYOL078W; YOL078W; YOL078W
GeneIDi854076
KEGGisce:YOL078W

Similar proteinsi

Entry informationi

Entry nameiAVO1_YEAST
AccessioniPrimary (citable) accession number: Q08236
Secondary accession number(s): D6W1Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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