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Protein

Nuclear mRNA export protein THP1

Gene

THP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SAC3-THP1 complex, which functions in transcription-coupled mRNA export from the nucleus to the cytoplasm. SAC3-THP1 functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), by association with components of the nuclear mRNA export machinery (MEX67-MTR2 and SUB2) in the nucleoplasm and the nucleoporin NUP1 at the nuclear basket. THP1 binds to RNA in vitro.3 Publications

GO - Molecular functioni

  • double-stranded DNA binding Source: SGD
  • RNA binding Source: SGD

GO - Biological processi

  • mRNA 3'-end processing Source: SGD
  • mRNA export from nucleus Source: SGD
  • nuclear retention of pre-mRNA at the site of transcription Source: SGD
  • poly(A)+ mRNA export from nucleus Source: GO_Central
  • posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • transcription-coupled nucleotide-excision repair Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33477-MONOMER.

Protein family/group databases

TCDBi3.A.22.1.1. the transcription-coupled trex/tap nuclear mrna export complex (trex) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear mRNA export protein THP1
Alternative name(s):
Bud site selection protein 29
Gene namesi
Name:THP1
Synonyms:BUD29
Ordered Locus Names:YOL072W
ORF Names:O1140
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL072W.
SGDiS000005433. THP1.

Subcellular locationi

GO - Cellular componenti

  • nuclear envelope Source: SGD
  • nucleus Source: SGD
  • transcription export complex 2 Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002706181 – 455Nuclear mRNA export protein THP1Add BLAST455

Proteomic databases

MaxQBiQ08231.
PRIDEiQ08231.

Interactioni

Subunit structurei

Heterodimer with THP1. The SAC3-THP1 complex interacts with CDC31 and SUS1, and with the mRNA export factor MEX67-MTR2, the TREX complex component SUB2, and the nucleoporin NUP1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC31P067043EBI-32097,EBI-4259

Protein-protein interaction databases

BioGridi34329. 160 interactors.
DIPiDIP-5060N.
IntActiQ08231. 5 interactors.
MINTiMINT-574152.

Structurei

Secondary structure

1455
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 13Combined sources10
Beta strandi23 – 26Combined sources4
Helixi27 – 39Combined sources13
Helixi42 – 51Combined sources10
Helixi59 – 74Combined sources16
Helixi84 – 95Combined sources12
Beta strandi98 – 100Combined sources3
Helixi106 – 129Combined sources24
Helixi131 – 134Combined sources4
Helixi140 – 155Combined sources16
Helixi170 – 188Combined sources19
Helixi192 – 194Combined sources3
Helixi195 – 200Combined sources6
Helixi202 – 205Combined sources4
Helixi211 – 213Combined sources3
Helixi216 – 232Combined sources17
Helixi236 – 252Combined sources17
Helixi258 – 277Combined sources20
Helixi284 – 287Combined sources4
Helixi288 – 290Combined sources3
Helixi293 – 308Combined sources16
Helixi311 – 320Combined sources10
Helixi322 – 327Combined sources6
Helixi331 – 354Combined sources24
Turni355 – 358Combined sources4
Beta strandi362 – 364Combined sources3
Helixi365 – 376Combined sources12
Turni390 – 392Combined sources3
Helixi397 – 399Combined sources3
Helixi400 – 409Combined sources10
Beta strandi415 – 418Combined sources4
Turni419 – 422Combined sources4
Beta strandi423 – 426Combined sources4
Helixi432 – 434Combined sources3
Helixi439 – 446Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T5VX-ray2.90B/E1-455[»]
4TRQX-ray3.10B/E170-455[»]
5L3TX-ray4.93B1-455[»]
ProteinModelPortaliQ08231.
SMRiQ08231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini297 – 428PCIAdd BLAST132

Sequence similaritiesi

Contains 1 PCI domain.Curated

Phylogenomic databases

HOGENOMiHOG000141936.
InParanoidiQ08231.
OMAiLNNIYFR.
OrthoDBiEOG092C45YA.

Family and domain databases

InterProiIPR000717. PCI_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDMANQLLDE LAHGNFSHLT LNLSQNGREI AILQKQLTGF DDKQLETFVE
60 70 80 90 100
QHPAMPNDTR FKIMCTSFLN YARDVDPWSA WSSSDLIFEF YQCLINCLIN
110 120 130 140 150
DNAPHIEMLI PVATRETEFI INLAGKLDSF HLQLHTRSHQ FLSHISSILS
160 170 180 190 200
RLFNSIKPPR GNASSTNIPG KQRILLYLVN KLNNIYFRIE SPQLCSNIFK
210 220 230 240 250
NFQPKSMLAH FNEYQLDQQI EYRYLLGRYY LLNSQVHNAF VQFNEAFQSL
260 270 280 290 300
LNLPLTNQAI TRNGTRILNY MIPTGLILGK MVKWGPLRPF LSQETIDNWS
310 320 330 340 350
VLYKHVRYGN IQGVSLWLRQ NERHLCARQL LIVLLEKLPM VTYRNLIKTV
360 370 380 390 400
IKSWTTEWGQ NKLPYSLIER VLQLSIGPTF EDPGAQEITI YNGIHSPKNV
410 420 430 440 450
ENVLVTLINL GLLRANCFPQ LQLCVVKKTT MIQEIVPPVN ERITKMFPAH

SHVLW
Length:455
Mass (Da):52,678
Last modified:November 1, 1996 - v1
Checksum:i7026F1F6FCC73FFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74814 Genomic DNA. Translation: CAA99082.1.
BK006948 Genomic DNA. Translation: DAA10711.1.
PIRiS66765.
RefSeqiNP_014569.1. NM_001183327.1.

Genome annotation databases

EnsemblFungiiYOL072W; YOL072W; YOL072W.
GeneIDi854082.
KEGGisce:YOL072W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74814 Genomic DNA. Translation: CAA99082.1.
BK006948 Genomic DNA. Translation: DAA10711.1.
PIRiS66765.
RefSeqiNP_014569.1. NM_001183327.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T5VX-ray2.90B/E1-455[»]
4TRQX-ray3.10B/E170-455[»]
5L3TX-ray4.93B1-455[»]
ProteinModelPortaliQ08231.
SMRiQ08231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34329. 160 interactors.
DIPiDIP-5060N.
IntActiQ08231. 5 interactors.
MINTiMINT-574152.

Protein family/group databases

TCDBi3.A.22.1.1. the transcription-coupled trex/tap nuclear mrna export complex (trex) family.

Proteomic databases

MaxQBiQ08231.
PRIDEiQ08231.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL072W; YOL072W; YOL072W.
GeneIDi854082.
KEGGisce:YOL072W.

Organism-specific databases

EuPathDBiFungiDB:YOL072W.
SGDiS000005433. THP1.

Phylogenomic databases

HOGENOMiHOG000141936.
InParanoidiQ08231.
OMAiLNNIYFR.
OrthoDBiEOG092C45YA.

Enzyme and pathway databases

BioCyciYEAST:G3O-33477-MONOMER.

Miscellaneous databases

PROiQ08231.

Family and domain databases

InterProiIPR000717. PCI_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHP1_YEAST
AccessioniPrimary (citable) accession number: Q08231
Secondary accession number(s): D6W1Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1140 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.