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Protein

Nuclear mRNA export protein THP1

Gene

THP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SAC3-THP1 complex, which functions in transcription-coupled mRNA export from the nucleus to the cytoplasm. SAC3-THP1 functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), by association with components of the nuclear mRNA export machinery (MEX67-MTR2 and SUB2) in the nucleoplasm and the nucleoporin NUP1 at the nuclear basket. THP1 binds to RNA in vitro.3 Publications

GO - Molecular functioni

  • double-stranded DNA binding Source: SGD
  • RNA binding Source: SGD

GO - Biological processi

  • mRNA 3'-end processing Source: SGD
  • mRNA export from nucleus Source: SGD
  • nuclear retention of pre-mRNA at the site of transcription Source: SGD
  • posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • transcription-coupled nucleotide-excision repair Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33477-MONOMER.

Protein family/group databases

TCDBi3.A.22.1.1. the transcription-coupled trex/tap nuclear mrna export complex (trex) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear mRNA export protein THP1
Alternative name(s):
Bud site selection protein 29
Gene namesi
Name:THP1
Synonyms:BUD29
Ordered Locus Names:YOL072W
ORF Names:O1140
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL072W.
SGDiS000005433. THP1.

Subcellular locationi

GO - Cellular componenti

  • nuclear envelope Source: SGD
  • nucleus Source: SGD
  • transcription export complex 2 Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 455455Nuclear mRNA export protein THP1PRO_0000270618Add
BLAST

Proteomic databases

MaxQBiQ08231.

Interactioni

Subunit structurei

Heterodimer with THP1. The SAC3-THP1 complex interacts with CDC31 and SUS1, and with the mRNA export factor MEX67-MTR2, the TREX complex component SUB2, and the nucleoporin NUP1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC31P067043EBI-32097,EBI-4259

Protein-protein interaction databases

BioGridi34329. 160 interactions.
DIPiDIP-5060N.
IntActiQ08231. 5 interactions.
MINTiMINT-574152.

Structurei

Secondary structure

1
455
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1310Combined sources
Beta strandi23 – 264Combined sources
Helixi27 – 3913Combined sources
Helixi42 – 5110Combined sources
Helixi59 – 7416Combined sources
Helixi84 – 9512Combined sources
Beta strandi98 – 1003Combined sources
Helixi106 – 12924Combined sources
Helixi131 – 1344Combined sources
Helixi140 – 15516Combined sources
Helixi170 – 18819Combined sources
Helixi192 – 1943Combined sources
Helixi195 – 2006Combined sources
Helixi202 – 2054Combined sources
Helixi211 – 2133Combined sources
Helixi216 – 23217Combined sources
Helixi236 – 25217Combined sources
Helixi258 – 27720Combined sources
Helixi284 – 2874Combined sources
Helixi288 – 2903Combined sources
Helixi293 – 30816Combined sources
Helixi311 – 32010Combined sources
Helixi322 – 3276Combined sources
Helixi331 – 35424Combined sources
Turni355 – 3584Combined sources
Beta strandi362 – 3643Combined sources
Helixi365 – 37612Combined sources
Turni390 – 3923Combined sources
Helixi397 – 3993Combined sources
Helixi400 – 40910Combined sources
Beta strandi415 – 4184Combined sources
Turni419 – 4224Combined sources
Beta strandi423 – 4264Combined sources
Helixi432 – 4343Combined sources
Helixi439 – 4468Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3T5VX-ray2.90B/E1-455[»]
4TRQX-ray3.10B/E170-455[»]
ProteinModelPortaliQ08231.
SMRiQ08231. Positions 2-455.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini297 – 428132PCIAdd
BLAST

Sequence similaritiesi

Contains 1 PCI domain.Curated

Phylogenomic databases

HOGENOMiHOG000141936.
InParanoidiQ08231.
OMAiLNNIYFR.
OrthoDBiEOG092C45YA.

Family and domain databases

InterProiIPR000717. PCI_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDMANQLLDE LAHGNFSHLT LNLSQNGREI AILQKQLTGF DDKQLETFVE
60 70 80 90 100
QHPAMPNDTR FKIMCTSFLN YARDVDPWSA WSSSDLIFEF YQCLINCLIN
110 120 130 140 150
DNAPHIEMLI PVATRETEFI INLAGKLDSF HLQLHTRSHQ FLSHISSILS
160 170 180 190 200
RLFNSIKPPR GNASSTNIPG KQRILLYLVN KLNNIYFRIE SPQLCSNIFK
210 220 230 240 250
NFQPKSMLAH FNEYQLDQQI EYRYLLGRYY LLNSQVHNAF VQFNEAFQSL
260 270 280 290 300
LNLPLTNQAI TRNGTRILNY MIPTGLILGK MVKWGPLRPF LSQETIDNWS
310 320 330 340 350
VLYKHVRYGN IQGVSLWLRQ NERHLCARQL LIVLLEKLPM VTYRNLIKTV
360 370 380 390 400
IKSWTTEWGQ NKLPYSLIER VLQLSIGPTF EDPGAQEITI YNGIHSPKNV
410 420 430 440 450
ENVLVTLINL GLLRANCFPQ LQLCVVKKTT MIQEIVPPVN ERITKMFPAH

SHVLW
Length:455
Mass (Da):52,678
Last modified:November 1, 1996 - v1
Checksum:i7026F1F6FCC73FFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74814 Genomic DNA. Translation: CAA99082.1.
BK006948 Genomic DNA. Translation: DAA10711.1.
PIRiS66765.
RefSeqiNP_014569.1. NM_001183327.1.

Genome annotation databases

EnsemblFungiiYOL072W; YOL072W; YOL072W.
GeneIDi854082.
KEGGisce:YOL072W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74814 Genomic DNA. Translation: CAA99082.1.
BK006948 Genomic DNA. Translation: DAA10711.1.
PIRiS66765.
RefSeqiNP_014569.1. NM_001183327.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3T5VX-ray2.90B/E1-455[»]
4TRQX-ray3.10B/E170-455[»]
ProteinModelPortaliQ08231.
SMRiQ08231. Positions 2-455.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34329. 160 interactions.
DIPiDIP-5060N.
IntActiQ08231. 5 interactions.
MINTiMINT-574152.

Protein family/group databases

TCDBi3.A.22.1.1. the transcription-coupled trex/tap nuclear mrna export complex (trex) family.

Proteomic databases

MaxQBiQ08231.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL072W; YOL072W; YOL072W.
GeneIDi854082.
KEGGisce:YOL072W.

Organism-specific databases

EuPathDBiFungiDB:YOL072W.
SGDiS000005433. THP1.

Phylogenomic databases

HOGENOMiHOG000141936.
InParanoidiQ08231.
OMAiLNNIYFR.
OrthoDBiEOG092C45YA.

Enzyme and pathway databases

BioCyciYEAST:G3O-33477-MONOMER.

Miscellaneous databases

PROiQ08231.

Family and domain databases

InterProiIPR000717. PCI_dom.
[Graphical view]
PfamiPF01399. PCI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHP1_YEAST
AccessioniPrimary (citable) accession number: Q08231
Secondary accession number(s): D6W1Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1140 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.