Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear mRNA export protein THP1

Gene

THP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SAC3-THP1 complex, which functions in transcription-coupled mRNA export from the nucleus to the cytoplasm. SAC3-THP1 functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), by association with components of the nuclear mRNA export machinery (MEX67-MTR2 and SUB2) in the nucleoplasm and the nucleoporin NUP1 at the nuclear basket. THP1 binds to RNA in vitro.3 Publications

Miscellaneous

Present with 1140 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • double-stranded DNA binding Source: SGD
  • RNA binding Source: SGD

GO - Biological processi

  • mRNA 3'-end processing Source: SGD
  • mRNA export from nucleus Source: SGD
  • nuclear retention of pre-mRNA at the site of transcription Source: SGD
  • poly(A)+ mRNA export from nucleus Source: GO_Central
  • posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • transcription-coupled nucleotide-excision repair Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD

Keywordsi

Biological processmRNA transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33477-MONOMER.

Protein family/group databases

TCDBi3.A.22.1.1. the transcription-coupled trex/tap nuclear mrna export complex (trex) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear mRNA export protein THP1
Alternative name(s):
Bud site selection protein 29
Gene namesi
Name:THP1
Synonyms:BUD29
Ordered Locus Names:YOL072W
ORF Names:O1140
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL072W.
SGDiS000005433. THP1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002706181 – 455Nuclear mRNA export protein THP1Add BLAST455

Proteomic databases

MaxQBiQ08231.
PRIDEiQ08231.

Interactioni

Subunit structurei

Heterodimer with THP1. The SAC3-THP1 complex interacts with CDC31 and SUS1, and with the mRNA export factor MEX67-MTR2, the TREX complex component SUB2, and the nucleoporin NUP1.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34329. 161 interactors.
DIPiDIP-5060N.
IntActiQ08231. 6 interactors.
MINTiMINT-574152.
STRINGi4932.YOL072W.

Structurei

Secondary structure

1455
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 12Combined sources10
Helixi23 – 37Combined sources15
Helixi42 – 51Combined sources10
Helixi59 – 74Combined sources16
Helixi80 – 96Combined sources17
Beta strandi98 – 100Combined sources3
Helixi106 – 128Combined sources23
Helixi131 – 134Combined sources4
Helixi140 – 155Combined sources16
Helixi170 – 173Combined sources4
Helixi175 – 188Combined sources14
Helixi192 – 194Combined sources3
Helixi195 – 205Combined sources11
Helixi211 – 213Combined sources3
Helixi216 – 232Combined sources17
Helixi236 – 251Combined sources16
Helixi258 – 277Combined sources20
Helixi285 – 290Combined sources6
Helixi293 – 308Combined sources16
Helixi311 – 320Combined sources10
Helixi322 – 327Combined sources6
Helixi331 – 354Combined sources24
Turni355 – 358Combined sources4
Beta strandi362 – 364Combined sources3
Helixi365 – 376Combined sources12
Turni390 – 392Combined sources3
Helixi397 – 399Combined sources3
Helixi400 – 409Combined sources10
Beta strandi416 – 418Combined sources3
Turni419 – 422Combined sources4
Beta strandi423 – 425Combined sources3
Helixi432 – 435Combined sources4
Helixi439 – 446Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T5VX-ray2.90B/E1-455[»]
4TRQX-ray3.10B/E170-455[»]
5G5Pelectron microscopy5.30B1-455[»]
5L3TX-ray4.93B1-455[»]
5UBPX-ray2.30B1-455[»]
ProteinModelPortaliQ08231.
SMRiQ08231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini220 – 431PCIPROSITE-ProRule annotationAdd BLAST212

Phylogenomic databases

HOGENOMiHOG000141936.
InParanoidiQ08231.
OMAiRNIFSNM.
OrthoDBiEOG092C45YA.

Family and domain databases

InterProiView protein in InterPro
IPR000717. PCI_dom.
PfamiView protein in Pfam
PF01399. PCI. 1 hit.
PROSITEiView protein in PROSITE
PS50250. PCI. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDMANQLLDE LAHGNFSHLT LNLSQNGREI AILQKQLTGF DDKQLETFVE
60 70 80 90 100
QHPAMPNDTR FKIMCTSFLN YARDVDPWSA WSSSDLIFEF YQCLINCLIN
110 120 130 140 150
DNAPHIEMLI PVATRETEFI INLAGKLDSF HLQLHTRSHQ FLSHISSILS
160 170 180 190 200
RLFNSIKPPR GNASSTNIPG KQRILLYLVN KLNNIYFRIE SPQLCSNIFK
210 220 230 240 250
NFQPKSMLAH FNEYQLDQQI EYRYLLGRYY LLNSQVHNAF VQFNEAFQSL
260 270 280 290 300
LNLPLTNQAI TRNGTRILNY MIPTGLILGK MVKWGPLRPF LSQETIDNWS
310 320 330 340 350
VLYKHVRYGN IQGVSLWLRQ NERHLCARQL LIVLLEKLPM VTYRNLIKTV
360 370 380 390 400
IKSWTTEWGQ NKLPYSLIER VLQLSIGPTF EDPGAQEITI YNGIHSPKNV
410 420 430 440 450
ENVLVTLINL GLLRANCFPQ LQLCVVKKTT MIQEIVPPVN ERITKMFPAH

SHVLW
Length:455
Mass (Da):52,678
Last modified:November 1, 1996 - v1
Checksum:i7026F1F6FCC73FFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74814 Genomic DNA. Translation: CAA99082.1.
BK006948 Genomic DNA. Translation: DAA10711.1.
PIRiS66765.
RefSeqiNP_014569.1. NM_001183327.1.

Genome annotation databases

EnsemblFungiiYOL072W; YOL072W; YOL072W.
GeneIDi854082.
KEGGisce:YOL072W.

Similar proteinsi

Entry informationi

Entry nameiTHP1_YEAST
AccessioniPrimary (citable) accession number: Q08231
Secondary accession number(s): D6W1Z5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: November 22, 2017
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names