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Protein

Succinate dehydrogenase assembly factor 2, mitochondrial

Gene

SDH5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit SDH1 of the SDH catalytic dimer. It is unclear whether it participates in the chemistry of FAD attachment (enzymatic function) or acts as a chaperone that maintains SDH1 in a conformation that is susceptible to autocatalytic FAD attachment (PubMed:19628817). Does not bind FAD or FADH2 in vitro (PubMed:23062074). Involved in sporulation (PubMed:12432101). Required for the full activation of the early meiotic inducer IME1 (PubMed:12586695).UniRule annotation4 Publications

Miscellaneous

Present with 1080 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • mitochondrial electron transport, succinate to ubiquinone Source: UniProtKB
  • mitochondrial respiratory chain complex II assembly Source: SGD
  • protein-FAD linkage Source: UniProtKB
  • protein flavinylation Source: UniProtKB
  • tricarboxylic acid cycle Source: SGD

Keywordsi

Molecular functionChaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-33476-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase assembly factor 2, mitochondrial1 PublicationUniRule annotation
Short name:
SDH assembly factor 2UniRule annotation
Short name:
SDHAF21 PublicationUniRule annotation
Gene namesi
Name:SDH51 PublicationUniRule annotation
Synonyms:EMI51 Publication
Ordered Locus Names:YOL071WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL071W
SGDiS000005432 SDH5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Respiratory deficient phenotype: viable on glucose medium, but is inviable on non-fermentable carbon sources such as glycerol. Cells lack SDH activity due to a complete loss of FAD cofactor attachment of SDH1.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi68R → E: Loss of covalent FAD in SDH1. 1 Publication1
Mutagenesisi71Y → D: Loss of covalent FAD in SDH1. 1 Publication1
Mutagenesisi98 – 99EE → KK: No effect. 1 Publication2
Mutagenesisi113W → A: Loss of covalent FAD in SDH1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35Mitochondrion1 PublicationAdd BLAST35
ChainiPRO_000000781436 – 162Succinate dehydrogenase assembly factor 2, mitochondrialAdd BLAST127

Proteomic databases

MaxQBiQ08230
PaxDbiQ08230
PRIDEiQ08230

Interactioni

Subunit structurei

Interacts with SDH1 within the SDH catalytic dimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi34330, 99 interactors
DIPiDIP-61727N
IntActiQ08230, 1 interactor
STRINGi4932.YOL071W

Structurei

Secondary structure

1162
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi62 – 73Combined sources12
Helixi79 – 95Combined sources17
Helixi98 – 108Combined sources11
Helixi112 – 119Combined sources8
Turni123 – 125Combined sources3
Helixi130 – 133Combined sources4
Helixi136 – 147Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LM4NMR-A55-152[»]
ProteinModelPortaliQ08230
SMRiQ08230
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SDHAF2 family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000001149
HOGENOMiHOG000230636
InParanoidiQ08230
KOiK18168
OMAiIYYWATD
OrthoDBiEOG092C5IJQ

Family and domain databases

Gene3Di1.10.150.250, 1 hit
HAMAPiMF_03057 SDHAF2, 1 hit
InterProiView protein in InterPro
IPR005631 SDH
IPR036714 SDH_sf
IPR028882 SDHAF2
PfamiView protein in Pfam
PF03937 Sdh5, 1 hit
SUPFAMiSSF109910 SSF109910, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNMFPALTK TLSLQGYKII NSQTGSAAWS CGRRWFSSDK DDHDDVVTRI
60 70 80 90 100
KIAPIKRTNE PLDKKRARLI YQSRKRGILE TDLLLSGFAA KYLKKMNEEE
110 120 130 140 150
LEEYDSLLNE LDWDIYYWAT KNFKTSPLPD KWANSKLLKQ LQEFSENKEK
160
EILSMPDLSK YQ
Length:162
Mass (Da):19,042
Last modified:November 1, 1996 - v1
Checksum:i8CDF8E943D46A12D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74813 Genomic DNA Translation: CAA99081.1
BK006948 Genomic DNA Translation: DAA10712.1
PIRiS66764
RefSeqiNP_014570.1, NM_001183326.1

Genome annotation databases

EnsemblFungiiYOL071W; YOL071W; YOL071W
GeneIDi854083
KEGGisce:YOL071W

Similar proteinsi

Entry informationi

Entry nameiSDHF2_YEAST
AccessioniPrimary (citable) accession number: Q08230
Secondary accession number(s): D6W1Z6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: February 28, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health