Q08229 (NBA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein NBA1

Alternative name(s):
NAP1 and bud neck-associated protein 1

Gene names

Name:	NBA1
Ordered Locus Names:	YOL070C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with NAP1 (via the central domain consisting of amino acids 143 to 362). Copurifies with ribosomes. Ref.8
Subcellular location	Bud neck. Cytoplasm. Note: Assembles at the inner surface of the cell membrane. Localizes to the septin cortex prior to bud emergence and to actin cap in unbudded cells. Ref.3 Ref.8
Post-translational modification	Phosphorylated by CDC28. Ref.5 Ref.6
Miscellaneous	Present with 6490 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cellular bud neck Inferred from direct assay Ref.3Ref.8. Source: SGD cytoplasm Inferred from direct assay Ref.3Ref.8. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NIS1	P53939	3	EBI-36841,EBI-28760

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Protein NBA1		PRO_0000235925
Regions
	Region	275 – 501	227	Necessary for the normal cellular distribution and bud neck targeting
	Compositional bias	405 – 424	20	Lys-rich
Amino acid modifications
	Modified residue	194	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q08229 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8128246D00B9C03D	FASTA	501	55,923
	10 20 30 40 50 60 MSEEREENGI SRATLNTQRL SAMIDSLNNE KDDRLFPSPT TTRTMITEEK ADQSDVFKPP 70 80 90 100 110 120 SRLLRSPAGD VSLPPGDNRS SMISNYSGII QEGVEVSYVV KNRQQTQERR TSKDSNSLYS 130 140 150 160 170 180 LKEPVSKNEL PSLPMLPSEA TLTKHLSDNQ STKSNTNADE IVIKPVTNAK PVGRFNSNTS 190 200 210 220 230 240 KKVEGRGSLK LLSSPLRQEK VMRSSIGSGN LASESGSSTY NTKFHQSIQE QLEEEEEGNV 250 260 270 280 290 300 SDKLSIVSSV IPELYTTTNE APKAINPIRS ETNDYNPTIP PRSKDRPRSR LFIEEGDGEG 310 320 330 340 350 360 DLLTEEILPT PVQPGGHYKN SSQISTVSEQ KSESYYSAAT SMPPEEETYL TRPLPSTPNE 370 380 390 400 410 420 DSRVTSNLKR DDTLKAIHDR ANHTSTSTNK QDDDMYEDII EETPKKTKLK KDTKKKLNKK 430 440 450 460 470 480 KSVKELRSFD IDTLNQLLSV TKGTLIGSEF AQLGMKIEEK RALERLVDSL SRLTADMVLD 490 500 PDRYEEGLKR LDKATKALEG F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed: 14574415] [Abstract] Cited for: PHOSPHORYLATION BY CDC28.
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-194, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes." Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J. Genes Dev. 20:1294-1307(2006) [PubMed: 16702403] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COPURIFICATION WITH RIBOSOMES.
[8]	"Phosphorylation by casein kinase 2 regulates Nap1 localization and function." Calvert M.E.K., Keck K.M., Ptak C., Shabanowitz J., Hunt D.F., Pemberton L.F. Mol. Cell. Biol. 28:1313-1325(2008) [PubMed: 18086883] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH NAP1, SUBCELLULAR LOCATION, REGION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74812 Genomic DNA. Translation: CAA99080.1. BK006948 Genomic DNA. Translation: DAA10713.1.
PIR	S66763.
RefSeq	NP_014571.1. NM_001183325.1.
3D structure databases
ProteinModelPortal	Q08229.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1680N.
IntAct	Q08229. 7 interactions.
MINT	MINT-403923.
STRING	Q08229.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOL070C; YOL070C; YOL070C.
GeneID	854084.
KEGG	sce:YOL070C.
NMPDR	fig|4932.3.peg.5663.
Organism-specific databases
CYGD	YOL070c.
SGD	S000005431. NBA1.
Phylogenomic databases
eggNOG	fuNOG10011.
HOGENOM	HBG202969.
OMA	TADMVLD.
OrthoDB	EOG4G7G7V.
Gene expression databases
ArrayExpress	Q08229.
Genevestigator	Q08229.
GermOnline	YOL070C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	975725.

Entry information

Entry name

NBA1_YEAST

Accession

Primary (citable) accession number: Q08229
Secondary accession number(s): D6W1Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program