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Protein

Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP54

Gene

INP54

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the phosphatidylinositol (4,5)-diphosphate levels on the cytoplasmic surface of the endoplasmic reticulum and thereby regulates secretion. Does not utilize phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2), nor phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 4-phosphate (PtdIns4P).2 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.

GO - Molecular functioni

  • phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Source: SGD

GO - Biological processi

  • phosphatidylinositol dephosphorylation Source: SGD
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-107.
YEAST:YOL065C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP54 (EC:3.1.3.36)
Gene namesi
Name:INP54
Ordered Locus Names:YOL065C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL065C.
SGDiS000005426. INP54.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384Phosphatidylinositol 4,5-bisphosphate 5-phosphatase INP54PRO_0000268682Add
BLAST

Proteomic databases

MaxQBiQ08227.
TopDownProteomicsiQ08227.

PTM databases

iPTMnetiQ08227.

Interactioni

Protein-protein interaction databases

BioGridi34336. 42 interactions.
DIPiDIP-2768N.
IntActiQ08227. 5 interactions.
MINTiMINT-566375.

Structurei

3D structure databases

ProteinModelPortaliQ08227.
SMRiQ08227. Positions 92-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000113076.
InParanoidiQ08227.
KOiK01106.
OMAiRQPSWCD.
OrthoDBiEOG7X9GH1.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SMARTiSM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08227-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKTNWKVSV TTFNCGKEFP VENSKAIVKQ LLFPYDDGIS QLELQDLYVL
60 70 80 90 100
GFQEVVPIWQ GSFPAVNRDL IDRITTTAVN CLNEKVSATQ GDEQYSCLGV
110 120 130 140 150
NSLGAITIIV LYNNNALKVK DDILKRNGKC GWFGTHLKGG TLISFQMTRN
160 170 180 190 200
GEENWERFSY ICAHLNANEG VNNRNQRIDD YKRIMSEVCD SEVAKSDHFF
210 220 230 240 250
FLGDLNFRVT STYDPTTNYS STTTLRRLLE NHEELNLLRK GEDEPLCKGF
260 270 280 290 300
QELKITFPPT YKFKLFEKET YNTKRIPSWC DRILYKSYAV PTFAQEGTYH
310 320 330 340 350
SVPRSNALLF SDHQPVNLTV RLPRSTGTPV PLSLHIEKYP LSWSSGLIGQ
360 370 380
IGDAVIGYCG WLVTKNVHYW ILGSLLLYLL LKIL
Length:384
Mass (Da):43,799
Last modified:November 1, 1996 - v1
Checksum:iC77711D57C64B365
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74807 Genomic DNA. Translation: CAA99075.1.
BK006948 Genomic DNA. Translation: DAA10718.1.
PIRiS66758.
RefSeqiNP_014576.1. NM_001183320.1.

Genome annotation databases

EnsemblFungiiYOL065C; YOL065C; YOL065C.
GeneIDi854089.
KEGGisce:YOL065C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74807 Genomic DNA. Translation: CAA99075.1.
BK006948 Genomic DNA. Translation: DAA10718.1.
PIRiS66758.
RefSeqiNP_014576.1. NM_001183320.1.

3D structure databases

ProteinModelPortaliQ08227.
SMRiQ08227. Positions 92-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34336. 42 interactions.
DIPiDIP-2768N.
IntActiQ08227. 5 interactions.
MINTiMINT-566375.

PTM databases

iPTMnetiQ08227.

Proteomic databases

MaxQBiQ08227.
TopDownProteomicsiQ08227.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL065C; YOL065C; YOL065C.
GeneIDi854089.
KEGGisce:YOL065C.

Organism-specific databases

EuPathDBiFungiDB:YOL065C.
SGDiS000005426. INP54.

Phylogenomic databases

HOGENOMiHOG000113076.
InParanoidiQ08227.
KOiK01106.
OMAiRQPSWCD.
OrthoDBiEOG7X9GH1.

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-107.
YEAST:YOL065C-MONOMER.

Miscellaneous databases

NextBioi975740.
PROiQ08227.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SMARTiSM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesion."
    Raucher D., Stauffer T., Chen W., Shen K., Guo S., York J.D., Sheetz M.P., Meyer T.
    Cell 100:221-228(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "The yeast inositol polyphosphate 5-phosphatase Inp54p localizes to the endoplasmic reticulum via a C-terminal hydrophobic anchoring tail: regulation of secretion from the endoplasmic reticulum."
    Wiradjaja F., Ooms L.M., Whisstock J.C., McColl B.K., Helfenbaum L., Sambrook J.F., Gething M.J., Mitchell C.A.
    J. Biol. Chem. 276:7643-7653(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiINP54_YEAST
AccessioniPrimary (citable) accession number: Q08227
Secondary accession number(s): D6W202
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.