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Q08224 (THI20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20

EC=2.7.1.49
EC=2.7.4.7
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name=HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name=HMP-P kinase
Short name=HMP-phosphate kinase
Short name=HMPP kinase
Gene names
Name:THI20
Ordered Locus Names:YOL055C
ORF Names:O1239
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and also probaby that of HMP to HMP-P. Ref.5

Catalytic activity

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.

ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 2/3.

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 3/3.

Induction

By absence of thiamine. Ref.5

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.

Sequence similarities

In the N-terminal section; belongs to the ThiD family.

In the C-terminal section; belongs to the thiaminase-2 family.

Sequence caution

The sequence CAA62531.1 differs from that shown. Reason: Frameshift at position 544.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 551551Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20
PRO_0000192041

Sites

Binding site641Substrate By similarity

Experimental info

Sequence conflict3231S → G in AAT92799. Ref.4

Secondary structure

........................................................................ 551
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q08224 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D57D1C6B693EE401

FASTA55161,269
        10         20         30         40         50         60 
MTYSTVSINT PPPYLTLACN EKLPTVLSIA GTDPSGGAGI EADVKTITAH RCYAMTCITA 

        70         80         90        100        110        120 
LNAQTPVKVY SINNTPKEVV FQTLESNLKD MKCNVIKTGM LTAAAIEVLH EKLLQLGENR 

       130        140        150        160        170        180 
PKLVVDPVLV ATSGSSLAGK DIVSLITEKV APFADILTPN IPECYKLLGE ERKVNGLQDI 

       190        200        210        220        230        240 
FQIAKDLAKI TKCSNILVKG GHIPWNDEKE KYITDVLFLG AEQKFIIFKG NFVNTTHTHG 

       250        260        270        280        290        300 
TGCTLASAIA SNLARGYSLP QSVYGGIEYV QNAVAIGCDV TKETVKDNGP INHVYAVEIP 

       310        320        330        340        350        360 
LEKMLSDECF TASDVIPKKP LKSAADKIPG GNFYEYLINH PKVKPHWDSY INHEFVKKVA 

       370        380        390        400        410        420 
DGTLERKKFQ FFIEQDYAYL VDYARVHCIA GSKAPCLEDM EKELVIVGGV RTEMGQHEKR 

       430        440        450        460        470        480 
LKEVFGVKDP DYFQKIKRGP ALRAYSRYFN DVSRRGNWQE LVASLTPCLM GYGEALTKMK 

       490        500        510        520        530        540 
GKVTAPEGSV YHEWCETYAS SWYREAMDEG EKLLNHILET YPPEQLDTLV TIYAEVCELE 

       550 
TNFWTAALEY E 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 26 kb region on the left arm of yeast chromosome XV."
Mannhaupt G., Vetter I., Schwarzlose C., Mitzel S., Feldmann H.
Yeast 12:67-76(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Genetic redundancy and gene fusion in the genome of the Baker's yeast Saccharomyces cerevisiae: functional characterization of a three-member gene family involved in the thiamine biosynthetic pathway."
Llorente B., Fairhead C., Dujon B.
Mol. Microbiol. 32:1140-1152(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A HMPP KINASE, INDUCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X91067 Genomic DNA. Translation: CAA62531.1. Frameshift.
Z74797 Genomic DNA. Translation: CAA99063.1.
AY692780 Genomic DNA. Translation: AAT92799.1.
BK006948 Genomic DNA. Translation: DAA10728.1.
PIRS66740.
RefSeqNP_014586.1. NM_001183310.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3RM5X-ray2.68A/B2-551[»]
ProteinModelPortalQ08224.
SMRQ08224. Positions 2-551.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34346. 16 interactions.
DIPDIP-8852N.
IntActQ08224. 1 interaction.

Proteomic databases

MaxQBQ08224.
PaxDbQ08224.
PeptideAtlasQ08224.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL055C; YOL055C; YOL055C.
GeneID854099.
KEGGsce:YOL055C.

Organism-specific databases

CYGDYOL055c.
SGDS000005416. THI20.

Phylogenomic databases

eggNOGCOG0351.
GeneTreeENSGT00390000003953.
HOGENOMHOG000225275.
KOK00877.
OMAHIAKESQ.
OrthoDBEOG75MW5D.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER3O-77.
YEAST:MONOMER3O-77.
UniPathwayUPA00060; UER00137.
UPA00060; UER00138.

Gene expression databases

GenevestigatorQ08224.

Family and domain databases

Gene3D1.20.910.10. 1 hit.
3.40.1190.20. 1 hit.
InterProIPR016084. Haem_Oase-like_multi-hlx.
IPR013749. HMP-P_kinase-1.
IPR004399. HMP-P_kinase-2.
IPR029056. Ribokinase-like.
IPR004305. Thiaminase-2/PQQC.
IPR027574. Thiaminase_II.
[Graphical view]
PfamPF08543. Phos_pyr_kin. 1 hit.
PF03070. TENA_THI-4. 1 hit.
[Graphical view]
SUPFAMSSF48613. SSF48613. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00097. HMP-P_kinase. 1 hit.
TIGR04306. salvage_TenA. 1 hit.
ProtoNetSearch...

Other

NextBio975770.

Entry information

Entry nameTHI20_YEAST
AccessionPrimary (citable) accession number: Q08224
Secondary accession number(s): D6W212, Q05664, Q6B2F0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways