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Q08224

- THI20_YEAST

UniProt

Q08224 - THI20_YEAST

Protein

Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20

Gene

THI20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and also probably that of HMP to HMP-P.1 Publication

    Catalytic activityi

    ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine.
    ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei64 – 641SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydroxymethylpyrimidine kinase activity Source: SGD
    3. phosphomethylpyrimidine kinase activity Source: SGD
    4. thiaminase activity Source: SGD

    GO - Biological processi

    1. thiamine biosynthetic process Source: SGD
    2. thiamine catabolic process Source: SGD
    3. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER3O-77.
    YEAST:MONOMER3O-77.
    UniPathwayiUPA00060; UER00137.
    UPA00060; UER00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20 (EC:2.7.1.49, EC:2.7.4.7)
    Alternative name(s):
    Hydroxymethylpyrimidine kinase
    Short name:
    HMP kinase
    Hydroxymethylpyrimidine phosphate kinase
    Short name:
    HMP-P kinase
    Short name:
    HMP-phosphate kinase
    Short name:
    HMPP kinase
    Gene namesi
    Name:THI20
    Ordered Locus Names:YOL055C
    ORF Names:O1239
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL055c.
    SGDiS000005416. THI20.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 551551Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20PRO_0000192041Add
    BLAST

    Proteomic databases

    MaxQBiQ08224.
    PaxDbiQ08224.
    PeptideAtlasiQ08224.

    Expressioni

    Inductioni

    By absence of thiamine.1 Publication

    Gene expression databases

    GenevestigatoriQ08224.

    Interactioni

    Protein-protein interaction databases

    BioGridi34346. 16 interactions.
    DIPiDIP-8852N.
    IntActiQ08224. 1 interaction.

    Structurei

    Secondary structure

    1
    551
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 75
    Helixi15 – 184
    Beta strandi25 – 317
    Beta strandi36 – 383
    Helixi39 – 4911
    Beta strandi53 – 6412
    Beta strandi69 – 746
    Helixi77 – 9014
    Beta strandi94 – 985
    Helixi103 – 11614
    Helixi117 – 1193
    Beta strandi122 – 1254
    Helixi142 – 1498
    Helixi151 – 1533
    Beta strandi155 – 1573
    Helixi161 – 1688
    Helixi178 – 19114
    Beta strandi196 – 2005
    Beta strandi212 – 2198
    Turni220 – 2234
    Beta strandi224 – 2318
    Helixi241 – 25414
    Helixi259 – 27618
    Turni293 – 2964
    Helixi300 – 3056
    Helixi333 – 3397
    Turni341 – 3433
    Helixi344 – 3518
    Helixi354 – 3607
    Helixi366 – 39328
    Helixi397 – 42327
    Turni430 – 4345
    Helixi440 – 45516
    Helixi458 – 47720
    Turni478 – 4814
    Helixi490 – 4978
    Helixi501 – 51818
    Helixi523 – 5253
    Helixi526 – 54823

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RM5X-ray2.68A/B2-551[»]
    ProteinModelPortaliQ08224.
    SMRiQ08224. Positions 2-551.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    In the N-terminal section; belongs to the ThiD family.Curated
    In the C-terminal section; belongs to the thiaminase-2 family.Curated

    Phylogenomic databases

    eggNOGiCOG0351.
    GeneTreeiENSGT00390000003953.
    HOGENOMiHOG000225275.
    KOiK00877.
    OMAiHIAKESQ.
    OrthoDBiEOG75MW5D.

    Family and domain databases

    Gene3Di1.20.910.10. 1 hit.
    3.40.1190.20. 1 hit.
    InterProiIPR016084. Haem_Oase-like_multi-hlx.
    IPR004399. HMP/HMP-P_kinase.
    IPR013749. PM/HMP-P_kinase-1.
    IPR029056. Ribokinase-like.
    IPR004305. Thiaminase-2/PQQC.
    IPR027574. Thiaminase_II.
    [Graphical view]
    PfamiPF08543. Phos_pyr_kin. 1 hit.
    PF03070. TENA_THI-4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48613. SSF48613. 1 hit.
    SSF53613. SSF53613. 1 hit.
    TIGRFAMsiTIGR00097. HMP-P_kinase. 1 hit.
    TIGR04306. salvage_TenA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q08224-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTYSTVSINT PPPYLTLACN EKLPTVLSIA GTDPSGGAGI EADVKTITAH    50
    RCYAMTCITA LNAQTPVKVY SINNTPKEVV FQTLESNLKD MKCNVIKTGM 100
    LTAAAIEVLH EKLLQLGENR PKLVVDPVLV ATSGSSLAGK DIVSLITEKV 150
    APFADILTPN IPECYKLLGE ERKVNGLQDI FQIAKDLAKI TKCSNILVKG 200
    GHIPWNDEKE KYITDVLFLG AEQKFIIFKG NFVNTTHTHG TGCTLASAIA 250
    SNLARGYSLP QSVYGGIEYV QNAVAIGCDV TKETVKDNGP INHVYAVEIP 300
    LEKMLSDECF TASDVIPKKP LKSAADKIPG GNFYEYLINH PKVKPHWDSY 350
    INHEFVKKVA DGTLERKKFQ FFIEQDYAYL VDYARVHCIA GSKAPCLEDM 400
    EKELVIVGGV RTEMGQHEKR LKEVFGVKDP DYFQKIKRGP ALRAYSRYFN 450
    DVSRRGNWQE LVASLTPCLM GYGEALTKMK GKVTAPEGSV YHEWCETYAS 500
    SWYREAMDEG EKLLNHILET YPPEQLDTLV TIYAEVCELE TNFWTAALEY 550
    E 551
    Length:551
    Mass (Da):61,269
    Last modified:November 1, 1996 - v1
    Checksum:iD57D1C6B693EE401
    GO

    Sequence cautioni

    The sequence CAA62531.1 differs from that shown. Reason: Frameshift at position 544.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti323 – 3231S → G in AAT92799. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91067 Genomic DNA. Translation: CAA62531.1. Frameshift.
    Z74797 Genomic DNA. Translation: CAA99063.1.
    AY692780 Genomic DNA. Translation: AAT92799.1.
    BK006948 Genomic DNA. Translation: DAA10728.1.
    PIRiS66740.
    RefSeqiNP_014586.1. NM_001183310.1.

    Genome annotation databases

    EnsemblFungiiYOL055C; YOL055C; YOL055C.
    GeneIDi854099.
    KEGGisce:YOL055C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X91067 Genomic DNA. Translation: CAA62531.1 . Frameshift.
    Z74797 Genomic DNA. Translation: CAA99063.1 .
    AY692780 Genomic DNA. Translation: AAT92799.1 .
    BK006948 Genomic DNA. Translation: DAA10728.1 .
    PIRi S66740.
    RefSeqi NP_014586.1. NM_001183310.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RM5 X-ray 2.68 A/B 2-551 [» ]
    ProteinModelPortali Q08224.
    SMRi Q08224. Positions 2-551.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34346. 16 interactions.
    DIPi DIP-8852N.
    IntActi Q08224. 1 interaction.

    Proteomic databases

    MaxQBi Q08224.
    PaxDbi Q08224.
    PeptideAtlasi Q08224.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL055C ; YOL055C ; YOL055C .
    GeneIDi 854099.
    KEGGi sce:YOL055C.

    Organism-specific databases

    CYGDi YOL055c.
    SGDi S000005416. THI20.

    Phylogenomic databases

    eggNOGi COG0351.
    GeneTreei ENSGT00390000003953.
    HOGENOMi HOG000225275.
    KOi K00877.
    OMAi HIAKESQ.
    OrthoDBi EOG75MW5D.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00137 .
    UPA00060 ; UER00138 .
    BioCyci MetaCyc:MONOMER3O-77.
    YEAST:MONOMER3O-77.

    Miscellaneous databases

    NextBioi 975770.

    Gene expression databases

    Genevestigatori Q08224.

    Family and domain databases

    Gene3Di 1.20.910.10. 1 hit.
    3.40.1190.20. 1 hit.
    InterProi IPR016084. Haem_Oase-like_multi-hlx.
    IPR004399. HMP/HMP-P_kinase.
    IPR013749. PM/HMP-P_kinase-1.
    IPR029056. Ribokinase-like.
    IPR004305. Thiaminase-2/PQQC.
    IPR027574. Thiaminase_II.
    [Graphical view ]
    Pfami PF08543. Phos_pyr_kin. 1 hit.
    PF03070. TENA_THI-4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48613. SSF48613. 1 hit.
    SSF53613. SSF53613. 1 hit.
    TIGRFAMsi TIGR00097. HMP-P_kinase. 1 hit.
    TIGR04306. salvage_TenA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a 26 kb region on the left arm of yeast chromosome XV."
      Mannhaupt G., Vetter I., Schwarzlose C., Mitzel S., Feldmann H.
      Yeast 12:67-76(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Genetic redundancy and gene fusion in the genome of the Baker's yeast Saccharomyces cerevisiae: functional characterization of a three-member gene family involved in the thiamine biosynthetic pathway."
      Llorente B., Fairhead C., Dujon B.
      Mol. Microbiol. 32:1140-1152(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A HMPP KINASE, INDUCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTHI20_YEAST
    AccessioniPrimary (citable) accession number: Q08224
    Secondary accession number(s): D6W212, Q05664, Q6B2F0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 195 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3