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Protein

Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20

Gene

THI20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and also probably that of HMP to HMP-P.1 Publication

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-2-methyl-5-(phosphomethyl)pyrimidine.Curated
ATP + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine = ADP + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine.Curated

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 2 and 3 of the subpathway that synthesizes 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21 (THI21), Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20 (THI20)
  3. Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI21 (THI21), Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20 (THI20)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64SubstrateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • hydroxymethylpyrimidine kinase activity Source: SGD
  • phosphomethylpyrimidine kinase activity Source: SGD
  • thiaminase activity Source: SGD

GO - Biological processi

  • thiamine biosynthetic process Source: SGD
  • thiamine catabolic process Source: SGD
  • thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-77
YEAST:MONOMER3O-77
BRENDAi2.7.1.49 984
2.7.4.7 984
3.5.99.2 984
UniPathwayiUPA00060; UER00137
UPA00060; UER00138

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20 (EC:2.7.1.49Curated, EC:2.7.4.7Curated)
Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name:
HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name:
HMP-P kinase
Short name:
HMP-phosphate kinase
Short name:
HMPP kinase
Gene namesi
Name:THI20
Ordered Locus Names:YOL055C
ORF Names:O1239
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL055C
SGDiS000005416 THI20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001920411 – 551Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20Add BLAST551

Proteomic databases

MaxQBiQ08224
PaxDbiQ08224
PRIDEiQ08224

Expressioni

Inductioni

By absence of thiamine.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi34346, 71 interactors
DIPiDIP-8852N
IntActiQ08224, 94 interactors
STRINGi4932.YOL055C

Structurei

Secondary structure

1551
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi15 – 18Combined sources4
Beta strandi25 – 31Combined sources7
Beta strandi36 – 38Combined sources3
Helixi39 – 49Combined sources11
Beta strandi53 – 64Combined sources12
Beta strandi69 – 74Combined sources6
Helixi77 – 90Combined sources14
Beta strandi94 – 98Combined sources5
Helixi103 – 116Combined sources14
Helixi117 – 119Combined sources3
Beta strandi122 – 125Combined sources4
Helixi142 – 149Combined sources8
Helixi151 – 153Combined sources3
Beta strandi155 – 157Combined sources3
Helixi161 – 168Combined sources8
Helixi178 – 191Combined sources14
Beta strandi196 – 200Combined sources5
Beta strandi212 – 219Combined sources8
Turni220 – 223Combined sources4
Beta strandi224 – 231Combined sources8
Helixi241 – 254Combined sources14
Helixi259 – 276Combined sources18
Turni293 – 296Combined sources4
Helixi300 – 305Combined sources6
Helixi333 – 339Combined sources7
Turni341 – 343Combined sources3
Helixi344 – 351Combined sources8
Helixi354 – 360Combined sources7
Helixi366 – 393Combined sources28
Helixi397 – 423Combined sources27
Turni430 – 434Combined sources5
Helixi440 – 455Combined sources16
Helixi458 – 477Combined sources20
Turni478 – 481Combined sources4
Helixi490 – 497Combined sources8
Helixi501 – 518Combined sources18
Helixi523 – 525Combined sources3
Helixi526 – 548Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RM5X-ray2.68A/B2-551[»]
ProteinModelPortaliQ08224
SMRiQ08224
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the ThiD family.Curated
In the C-terminal section; belongs to the thiaminase-2 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003953
HOGENOMiHOG000225275
InParanoidiQ08224
KOiK00877
OMAiGFWEMFP
OrthoDBiEOG092C1B4A

Family and domain databases

CDDicd01169 HMPP_kinase, 1 hit
Gene3Di1.20.910.10, 1 hit
3.40.1190.20, 1 hit
InterProiView protein in InterPro
IPR016084 Haem_Oase-like_multi-hlx
IPR004399 HMP/HMP-P_kinase
IPR013749 PM/HMP-P_kinase-1
IPR029056 Ribokinase-like
IPR004305 Thiaminase-2/PQQC
IPR027574 Thiaminase_II
PfamiView protein in Pfam
PF08543 Phos_pyr_kin, 1 hit
PF03070 TENA_THI-4, 1 hit
SUPFAMiSSF48613 SSF48613, 1 hit
SSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00097 HMP-P_kinase, 1 hit
TIGR04306 salvage_TenA, 1 hit

Sequencei

Sequence statusi: Complete.

Q08224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYSTVSINT PPPYLTLACN EKLPTVLSIA GTDPSGGAGI EADVKTITAH
60 70 80 90 100
RCYAMTCITA LNAQTPVKVY SINNTPKEVV FQTLESNLKD MKCNVIKTGM
110 120 130 140 150
LTAAAIEVLH EKLLQLGENR PKLVVDPVLV ATSGSSLAGK DIVSLITEKV
160 170 180 190 200
APFADILTPN IPECYKLLGE ERKVNGLQDI FQIAKDLAKI TKCSNILVKG
210 220 230 240 250
GHIPWNDEKE KYITDVLFLG AEQKFIIFKG NFVNTTHTHG TGCTLASAIA
260 270 280 290 300
SNLARGYSLP QSVYGGIEYV QNAVAIGCDV TKETVKDNGP INHVYAVEIP
310 320 330 340 350
LEKMLSDECF TASDVIPKKP LKSAADKIPG GNFYEYLINH PKVKPHWDSY
360 370 380 390 400
INHEFVKKVA DGTLERKKFQ FFIEQDYAYL VDYARVHCIA GSKAPCLEDM
410 420 430 440 450
EKELVIVGGV RTEMGQHEKR LKEVFGVKDP DYFQKIKRGP ALRAYSRYFN
460 470 480 490 500
DVSRRGNWQE LVASLTPCLM GYGEALTKMK GKVTAPEGSV YHEWCETYAS
510 520 530 540 550
SWYREAMDEG EKLLNHILET YPPEQLDTLV TIYAEVCELE TNFWTAALEY

E
Length:551
Mass (Da):61,269
Last modified:November 1, 1996 - v1
Checksum:iD57D1C6B693EE401
GO

Sequence cautioni

The sequence CAA62531 differs from that shown. Reason: Frameshift at position 544.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti323S → G in AAT92799 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91067 Genomic DNA Translation: CAA62531.1 Frameshift.
Z74797 Genomic DNA Translation: CAA99063.1
AY692780 Genomic DNA Translation: AAT92799.1
BK006948 Genomic DNA Translation: DAA10728.1
PIRiS66740
RefSeqiNP_014586.1, NM_001183310.1

Genome annotation databases

EnsemblFungiiYOL055C; YOL055C; YOL055C
GeneIDi854099
KEGGisce:YOL055C

Similar proteinsi

Entry informationi

Entry nameiTHI20_YEAST
AccessioniPrimary (citable) accession number: Q08224
Secondary accession number(s): D6W212, Q05664, Q6B2F0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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