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Protein

Glutathione synthetase

Gene

GSH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei128 – 1281Substrate1 Publication
Metal bindingi146 – 1461Magnesium
Binding sitei146 – 1461ATP
Metal bindingi148 – 1481MagnesiumBy similarity
Binding sitei234 – 2341Substrate1 Publication
Binding sitei324 – 3241ATP
Metal bindingi386 – 3861Magnesium
Binding sitei393 – 3931ATP
Binding sitei442 – 4421ATP
Binding sitei467 – 4671SubstrateBy similarity
Binding sitei469 – 4691ATP
Binding sitei475 – 4751ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi382 – 39110ATP
Nucleotide bindingi415 – 4184ATP

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. glutathione binding Source: UniProtKB
  3. glutathione synthase activity Source: SGD
  4. magnesium ion binding Source: UniProtKB
  5. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. glutathione biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YOL049W-MONOMER.
ReactomeiREACT_256904. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:GSH2
Ordered Locus Names:YOL049W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL049w.
SGDiS000005409. GSH2.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Glutathione synthetasePRO_0000211266Add
BLAST

Proteomic databases

MaxQBiQ08220.
PaxDbiQ08220.
PeptideAtlasiQ08220.

Expressioni

Gene expression databases

GenevestigatoriQ08220.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-7915,EBI-7915
SSA2P105921EBI-7915,EBI-8603

Protein-protein interaction databases

BioGridi34354. 46 interactions.
IntActiQ08220. 1 interaction.
MINTiMINT-625950.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2518Combined sources
Helixi34 – 363Combined sources
Beta strandi40 – 434Combined sources
Beta strandi46 – 494Combined sources
Beta strandi51 – 544Combined sources
Helixi55 – 7925Combined sources
Helixi85 – 9612Combined sources
Turni97 – 1004Combined sources
Helixi101 – 11111Combined sources
Beta strandi123 – 13614Combined sources
Beta strandi139 – 14810Combined sources
Helixi155 – 17016Combined sources
Helixi183 – 1853Combined sources
Helixi191 – 20818Combined sources
Beta strandi219 – 2246Combined sources
Helixi231 – 24313Combined sources
Beta strandi249 – 2524Combined sources
Helixi254 – 2607Combined sources
Beta strandi261 – 2633Combined sources
Turni265 – 2673Combined sources
Beta strandi270 – 2723Combined sources
Turni273 – 2753Combined sources
Beta strandi278 – 2869Combined sources
Helixi290 – 2923Combined sources
Helixi296 – 30712Combined sources
Beta strandi308 – 3136Combined sources
Helixi316 – 3216Combined sources
Helixi324 – 3296Combined sources
Helixi333 – 3364Combined sources
Turni337 – 3393Combined sources
Helixi343 – 3508Combined sources
Beta strandi356 – 3583Combined sources
Beta strandi360 – 3623Combined sources
Helixi363 – 37412Combined sources
Helixi376 – 3783Combined sources
Beta strandi379 – 3857Combined sources
Helixi394 – 3963Combined sources
Helixi397 – 4026Combined sources
Helixi406 – 4116Combined sources
Beta strandi412 – 4165Combined sources
Beta strandi428 – 4303Combined sources
Beta strandi433 – 4353Combined sources
Beta strandi439 – 45113Combined sources
Beta strandi456 – 46914Combined sources
Beta strandi472 – 4743Combined sources
Beta strandi477 – 4804Combined sources
Beta strandi483 – 4853Combined sources
Beta strandi488 – 4914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M0TX-ray2.30A/B1-491[»]
1M0WX-ray1.80A/B1-491[»]
ProteinModelPortaliQ08220.
SMRiQ08220. Positions 6-491.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08220.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1534Substrate binding
Regioni228 – 2303Substrate binding
Regioni285 – 2884Substrate binding
Regioni478 – 4792Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

eggNOGiNOG329040.
GeneTreeiENSGT00390000013764.
HOGENOMiHOG000172641.
InParanoidiQ08220.
KOiK01920.
OMAiEGRNIAT.
OrthoDBiEOG7GBG71.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHYPPSKDQ LNELIQEVNQ WAITNGLSMY PPKFEENPSN ASVSPVTIYP
60 70 80 90 100
TPIPRKCFDE AVQIQPVFNE LYARITQDMA QPDSYLHKTT EALALSDSEF
110 120 130 140 150
TGKLWSLYLA TLKSAQYKKQ NFRLGIFRSD YLIDKKKGTE QIKQVEFNTV
160 170 180 190 200
SVSFAGLSEK VDRLHSYLNR ANKYDPKGPI YNDQNMVISD SGYLLSKALA
210 220 230 240 250
KAVESYKSQQ SSSTTSDPIV AFIVQRNERN VFDQKVLELN LLEKFGTKSV
260 270 280 290 300
RLTFDDVNDK LFIDDKTGKL FIRDTEQEIA VVYYRTGYTT TDYTSEKDWE
310 320 330 340 350
ARLFLEKSFA IKAPDLLTQL SGSKKIQQLL TDEGVLGKYI SDAEKKSSLL
360 370 380 390 400
KTFVKIYPLD DTKLGREGKR LALSEPSKYV LKPQREGGGN NVYKENIPNF
410 420 430 440 450
LKGIEERHWD AYILMELIEP ELNENNIILR DNKSYNEPII SELGIYGCVL
460 470 480 490
FNDEQVLSNE FSGSLLRSKF NTSNEGGVAA GFGCLDSIIL Y
Length:491
Mass (Da):55,815
Last modified:November 1, 1996 - v1
Checksum:iD2AE50A487E0791C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13804 Genomic DNA. Translation: CAA74136.1.
Z74791 Genomic DNA. Translation: CAA99054.1.
AY723864 Genomic DNA. Translation: AAU09781.1.
BK006948 Genomic DNA. Translation: DAA10734.1.
PIRiS66734.
RefSeqiNP_014593.1. NM_001183303.1.

Genome annotation databases

EnsemblFungiiYOL049W; YOL049W; YOL049W.
GeneIDi854108.
KEGGisce:YOL049W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13804 Genomic DNA. Translation: CAA74136.1.
Z74791 Genomic DNA. Translation: CAA99054.1.
AY723864 Genomic DNA. Translation: AAU09781.1.
BK006948 Genomic DNA. Translation: DAA10734.1.
PIRiS66734.
RefSeqiNP_014593.1. NM_001183303.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1M0TX-ray2.30A/B1-491[»]
1M0WX-ray1.80A/B1-491[»]
ProteinModelPortaliQ08220.
SMRiQ08220. Positions 6-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34354. 46 interactions.
IntActiQ08220. 1 interaction.
MINTiMINT-625950.

Proteomic databases

MaxQBiQ08220.
PaxDbiQ08220.
PeptideAtlasiQ08220.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL049W; YOL049W; YOL049W.
GeneIDi854108.
KEGGisce:YOL049W.

Organism-specific databases

CYGDiYOL049w.
SGDiS000005409. GSH2.

Phylogenomic databases

eggNOGiNOG329040.
GeneTreeiENSGT00390000013764.
HOGENOMiHOG000172641.
InParanoidiQ08220.
KOiK01920.
OMAiEGRNIAT.
OrthoDBiEOG7GBG71.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.
BioCyciYEAST:YOL049W-MONOMER.
ReactomeiREACT_256904. Glutathione synthesis and recycling.

Miscellaneous databases

EvolutionaryTraceiQ08220.
NextBioi975792.
PROiQ08220.

Gene expression databases

GenevestigatoriQ08220.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular identification of glutathione synthetase (GSH2) gene from Saccharomyces cerevisiae."
    Inoue Y., Sugiyama K.I., Izawa S., Kimura A.
    Biochim. Biophys. Acta 1395:315-320(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large conformational changes in the catalytic cycle of glutathione synthase."
    Gogos A., Shapiro L.
    Structure 10:1669-1676(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND AMP-PNP, COFACTOR, SUBUNIT.

Entry informationi

Entry nameiGSHB_YEAST
AccessioniPrimary (citable) accession number: Q08220
Secondary accession number(s): D6W218
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3430 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.