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Q08220

- GSHB_YEAST

UniProt

Q08220 - GSHB_YEAST

Protein

Glutathione synthetase

Gene

GSH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

    Cofactori

    Binds 1 magnesium ion per subunit.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei128 – 1281Substrate1 Publication
    Metal bindingi146 – 1461Magnesium
    Binding sitei146 – 1461ATP
    Metal bindingi148 – 1481MagnesiumBy similarity
    Binding sitei234 – 2341Substrate1 Publication
    Binding sitei324 – 3241ATP
    Metal bindingi386 – 3861Magnesium
    Binding sitei393 – 3931ATP
    Binding sitei442 – 4421ATP
    Binding sitei467 – 4671SubstrateBy similarity
    Binding sitei469 – 4691ATP
    Binding sitei475 – 4751ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi382 – 39110ATP
    Nucleotide bindingi415 – 4184ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. glutathione binding Source: UniProtKB
    3. glutathione synthase activity Source: SGD
    4. magnesium ion binding Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. glutathione biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YOL049W-MONOMER.
    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetase (EC:6.3.2.3)
    Short name:
    GSH synthetase
    Short name:
    GSH-S
    Alternative name(s):
    Glutathione synthase
    Gene namesi
    Name:GSH2
    Ordered Locus Names:YOL049W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL049w.
    SGDiS000005409. GSH2.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491Glutathione synthetasePRO_0000211266Add
    BLAST

    Proteomic databases

    MaxQBiQ08220.
    PaxDbiQ08220.
    PeptideAtlasiQ08220.

    Expressioni

    Gene expression databases

    GenevestigatoriQ08220.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-7915,EBI-7915
    SSA2P105921EBI-7915,EBI-8603

    Protein-protein interaction databases

    BioGridi34354. 45 interactions.
    IntActiQ08220. 1 interaction.
    MINTiMINT-625950.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 2518
    Helixi34 – 363
    Beta strandi40 – 434
    Beta strandi46 – 494
    Beta strandi51 – 544
    Helixi55 – 7925
    Helixi85 – 9612
    Turni97 – 1004
    Helixi101 – 11111
    Beta strandi123 – 13614
    Beta strandi139 – 14810
    Helixi155 – 17016
    Helixi183 – 1853
    Helixi191 – 20818
    Beta strandi219 – 2246
    Helixi231 – 24313
    Beta strandi249 – 2524
    Helixi254 – 2607
    Beta strandi261 – 2633
    Turni265 – 2673
    Beta strandi270 – 2723
    Turni273 – 2753
    Beta strandi278 – 2869
    Helixi290 – 2923
    Helixi296 – 30712
    Beta strandi308 – 3136
    Helixi316 – 3216
    Helixi324 – 3296
    Helixi333 – 3364
    Turni337 – 3393
    Helixi343 – 3508
    Beta strandi356 – 3583
    Beta strandi360 – 3623
    Helixi363 – 37412
    Helixi376 – 3783
    Beta strandi379 – 3857
    Helixi394 – 3963
    Helixi397 – 4026
    Helixi406 – 4116
    Beta strandi412 – 4165
    Beta strandi428 – 4303
    Beta strandi433 – 4353
    Beta strandi439 – 45113
    Beta strandi456 – 46914
    Beta strandi472 – 4743
    Beta strandi477 – 4804
    Beta strandi483 – 4853
    Beta strandi488 – 4914

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1M0TX-ray2.30A/B1-491[»]
    1M0WX-ray1.80A/B1-491[»]
    ProteinModelPortaliQ08220.
    SMRiQ08220. Positions 6-491.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ08220.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni150 – 1534Substrate binding
    Regioni228 – 2303Substrate binding
    Regioni285 – 2884Substrate binding
    Regioni478 – 4792Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the eukaryotic GSH synthase family.Curated

    Phylogenomic databases

    eggNOGiNOG329040.
    GeneTreeiENSGT00390000013764.
    HOGENOMiHOG000172641.
    KOiK01920.
    OMAiENTAHAR.
    OrthoDBiEOG7GBG71.

    Family and domain databases

    Gene3Di1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 1 hit.
    3.40.50.1760. 1 hit.
    InterProiIPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR11130. PTHR11130. 1 hit.
    PfamiPF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001558. GSHase. 1 hit.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q08220-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAHYPPSKDQ LNELIQEVNQ WAITNGLSMY PPKFEENPSN ASVSPVTIYP    50
    TPIPRKCFDE AVQIQPVFNE LYARITQDMA QPDSYLHKTT EALALSDSEF 100
    TGKLWSLYLA TLKSAQYKKQ NFRLGIFRSD YLIDKKKGTE QIKQVEFNTV 150
    SVSFAGLSEK VDRLHSYLNR ANKYDPKGPI YNDQNMVISD SGYLLSKALA 200
    KAVESYKSQQ SSSTTSDPIV AFIVQRNERN VFDQKVLELN LLEKFGTKSV 250
    RLTFDDVNDK LFIDDKTGKL FIRDTEQEIA VVYYRTGYTT TDYTSEKDWE 300
    ARLFLEKSFA IKAPDLLTQL SGSKKIQQLL TDEGVLGKYI SDAEKKSSLL 350
    KTFVKIYPLD DTKLGREGKR LALSEPSKYV LKPQREGGGN NVYKENIPNF 400
    LKGIEERHWD AYILMELIEP ELNENNIILR DNKSYNEPII SELGIYGCVL 450
    FNDEQVLSNE FSGSLLRSKF NTSNEGGVAA GFGCLDSIIL Y 491
    Length:491
    Mass (Da):55,815
    Last modified:November 1, 1996 - v1
    Checksum:iD2AE50A487E0791C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13804 Genomic DNA. Translation: CAA74136.1.
    Z74791 Genomic DNA. Translation: CAA99054.1.
    AY723864 Genomic DNA. Translation: AAU09781.1.
    BK006948 Genomic DNA. Translation: DAA10734.1.
    PIRiS66734.
    RefSeqiNP_014593.1. NM_001183303.1.

    Genome annotation databases

    EnsemblFungiiYOL049W; YOL049W; YOL049W.
    GeneIDi854108.
    KEGGisce:YOL049W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13804 Genomic DNA. Translation: CAA74136.1 .
    Z74791 Genomic DNA. Translation: CAA99054.1 .
    AY723864 Genomic DNA. Translation: AAU09781.1 .
    BK006948 Genomic DNA. Translation: DAA10734.1 .
    PIRi S66734.
    RefSeqi NP_014593.1. NM_001183303.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1M0T X-ray 2.30 A/B 1-491 [» ]
    1M0W X-ray 1.80 A/B 1-491 [» ]
    ProteinModelPortali Q08220.
    SMRi Q08220. Positions 6-491.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34354. 45 interactions.
    IntActi Q08220. 1 interaction.
    MINTi MINT-625950.

    Proteomic databases

    MaxQBi Q08220.
    PaxDbi Q08220.
    PeptideAtlasi Q08220.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL049W ; YOL049W ; YOL049W .
    GeneIDi 854108.
    KEGGi sce:YOL049W.

    Organism-specific databases

    CYGDi YOL049w.
    SGDi S000005409. GSH2.

    Phylogenomic databases

    eggNOGi NOG329040.
    GeneTreei ENSGT00390000013764.
    HOGENOMi HOG000172641.
    KOi K01920.
    OMAi ENTAHAR.
    OrthoDBi EOG7GBG71.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .
    BioCyci YEAST:YOL049W-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q08220.
    NextBioi 975792.
    PROi Q08220.

    Gene expression databases

    Genevestigatori Q08220.

    Family and domain databases

    Gene3Di 1.10.1080.10. 2 hits.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 1 hit.
    3.40.50.1760. 1 hit.
    InterProi IPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR11130. PTHR11130. 1 hit.
    Pfami PF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001558. GSHase. 1 hit.
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular identification of glutathione synthetase (GSH2) gene from Saccharomyces cerevisiae."
      Inoue Y., Sugiyama K.I., Izawa S., Kimura A.
      Biochim. Biophys. Acta 1395:315-320(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Large conformational changes in the catalytic cycle of glutathione synthase."
      Gogos A., Shapiro L.
      Structure 10:1669-1676(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND AMP-PNP, COFACTOR, SUBUNIT.

    Entry informationi

    Entry nameiGSHB_YEAST
    AccessioniPrimary (citable) accession number: Q08220
    Secondary accession number(s): D6W218
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3430 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3