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Q08220 (GSHB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

Short name=GSH synthetase
Short name=GSH-S
EC=6.3.2.3
Alternative name(s):
Glutathione synthase
Gene names
Name:GSH2
Ordered Locus Names:YOL049W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactor

Binds 1 magnesium ion per subunit. Ref.6

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.

Subunit structure

Homodimer. Ref.6

Miscellaneous

Present with 3430 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the eukaryotic GSH synthase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-7915,EBI-7915
SSA2P105921EBI-7915,EBI-8603

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Glutathione synthetase
PRO_0000211266

Regions

Nucleotide binding382 – 39110ATP
Nucleotide binding415 – 4184ATP
Region150 – 1534Substrate binding
Region228 – 2303Substrate binding
Region285 – 2884Substrate binding
Region478 – 4792Substrate binding By similarity

Sites

Metal binding1461Magnesium
Metal binding1481Magnesium By similarity
Metal binding3861Magnesium
Binding site1281Substrate
Binding site1461ATP
Binding site2341Substrate
Binding site3241ATP
Binding site3931ATP
Binding site4421ATP
Binding site4671Substrate By similarity
Binding site4691ATP
Binding site4751ATP; via carbonyl oxygen By similarity

Secondary structure

..................................................................................... 491
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q08220 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D2AE50A487E0791C

FASTA49155,815
        10         20         30         40         50         60 
MAHYPPSKDQ LNELIQEVNQ WAITNGLSMY PPKFEENPSN ASVSPVTIYP TPIPRKCFDE 

        70         80         90        100        110        120 
AVQIQPVFNE LYARITQDMA QPDSYLHKTT EALALSDSEF TGKLWSLYLA TLKSAQYKKQ 

       130        140        150        160        170        180 
NFRLGIFRSD YLIDKKKGTE QIKQVEFNTV SVSFAGLSEK VDRLHSYLNR ANKYDPKGPI 

       190        200        210        220        230        240 
YNDQNMVISD SGYLLSKALA KAVESYKSQQ SSSTTSDPIV AFIVQRNERN VFDQKVLELN 

       250        260        270        280        290        300 
LLEKFGTKSV RLTFDDVNDK LFIDDKTGKL FIRDTEQEIA VVYYRTGYTT TDYTSEKDWE 

       310        320        330        340        350        360 
ARLFLEKSFA IKAPDLLTQL SGSKKIQQLL TDEGVLGKYI SDAEKKSSLL KTFVKIYPLD 

       370        380        390        400        410        420 
DTKLGREGKR LALSEPSKYV LKPQREGGGN NVYKENIPNF LKGIEERHWD AYILMELIEP 

       430        440        450        460        470        480 
ELNENNIILR DNKSYNEPII SELGIYGCVL FNDEQVLSNE FSGSLLRSKF NTSNEGGVAA 

       490 
GFGCLDSIIL Y 

« Hide

References

« Hide 'large scale' references
[1]"Molecular identification of glutathione synthetase (GSH2) gene from Saccharomyces cerevisiae."
Inoue Y., Sugiyama K.I., Izawa S., Kimura A.
Biochim. Biophys. Acta 1395:315-320(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large conformational changes in the catalytic cycle of glutathione synthase."
Gogos A., Shapiro L.
Structure 10:1669-1676(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND AMP-PNP, COFACTOR, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y13804 Genomic DNA. Translation: CAA74136.1.
Z74791 Genomic DNA. Translation: CAA99054.1.
AY723864 Genomic DNA. Translation: AAU09781.1.
BK006948 Genomic DNA. Translation: DAA10734.1.
PIRS66734.
RefSeqNP_014593.1. NM_001183303.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1M0TX-ray2.30A/B1-491[»]
1M0WX-ray1.80A/B1-491[»]
ProteinModelPortalQ08220.
SMRQ08220. Positions 6-491.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34354. 45 interactions.
IntActQ08220. 1 interaction.
MINTMINT-625950.

Proteomic databases

PaxDbQ08220.
PeptideAtlasQ08220.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL049W; YOL049W; YOL049W.
GeneID854108.
KEGGsce:YOL049W.

Organism-specific databases

CYGDYOL049w.
SGDS000005409. GSH2.

Phylogenomic databases

eggNOGNOG329040.
GeneTreeENSGT00390000013764.
HOGENOMHOG000172641.
KOK01920.
OMAPSHRDED.
OrthoDBEOG7GBG71.

Enzyme and pathway databases

BioCycYEAST:YOL049W-MONOMER.
UniPathwayUPA00142; UER00210.

Gene expression databases

GenevestigatorQ08220.

Family and domain databases

Gene3D1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR11130. PTHR11130. 1 hit.
PfamPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001558. GSHase. 1 hit.
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01986. glut_syn_euk. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ08220.
NextBio975792.
PROQ08220.

Entry information

Entry nameGSHB_YEAST
AccessionPrimary (citable) accession number: Q08220
Secondary accession number(s): D6W218
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways