Reviewed, UniProtKB/Swiss-Prot Q08220 (GSHB_YEAST)

Names and origin

Protein names

Recommended name:
    Glutathione synthetase
    EC=6.3.2.3
Alternative name(s):
    Glutathione synthase
    GSH synthetase
      Short name=GSH-S

Gene names

Name:	GSH2
Ordered Locus Names:	YOL049W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.
Miscellaneous	Present with 3430 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the eukaryotic GSH synthase family.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Ref.1 Inferred from mutant phenotype. Source: SGD response to heat Inferred from expression pattern. Source: SGD
Cellular component	intracellular Traceable author statement. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutathione synthase activity Ref.1 Inferred from direct assay. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-7915,EBI-7915
SSA2	P10592	1	EBI-7915,EBI-8603

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 491

491

Glutathione synthetase

PRO_0000211266

Amino acid modifications

Modified residue

42

1

Phosphoserine Ref.5

Secondary structure

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491

Helix Strand Turn

Details...

Helix

8 – 24

17

Helix

34 – 36

3

Beta strand

40 – 43

4

Beta strand

46 – 49

4

Beta strand

51 – 54

4

Helix

55 – 79

25

Helix

101 – 111

11

Beta strand

123 – 134

12

Beta strand

141 – 148

8

Helix

155 – 170

16

Helix

183 – 185

3

Helix

191 – 208

18

Beta strand

219 – 224

6

Helix

231 – 245

15

Beta strand

249 – 252

4

Helix

254 – 260

7

Beta strand

261 – 263

3

Turn

265 – 267

3

Beta strand

270 – 272

3

Turn

273 – 275

3

Beta strand

278 – 286

9

Helix

290 – 292

3

Helix

296 – 307

12

Beta strand

308 – 313

6

Helix

316 – 320

5

Helix

324 – 329

6

Helix

333 – 337

5

Helix

343 – 352

10

Beta strand

356 – 358

3

Beta strand

360 – 362

3

Helix

363 – 374

12

Helix

376 – 378

3

Beta strand

379 – 383

5

Helix

394 – 396

3

Helix

397 – 402

6

Helix

406 – 411

6

Beta strand

412 – 416

5

Beta strand

428 – 430

3

Beta strand

433 – 435

3

Beta strand

439 – 451

13

Beta strand

456 – 469

14

Beta strand

472 – 474

3

Beta strand

477 – 480

4

Beta strand

488 – 491

4

Sequences

	Sequence		Length	Mass (Da)
	Q08220-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D2AE50A487E0791C	FASTA	491	55,815
	10 20 30 40 50 60 MAHYPPSKDQ LNELIQEVNQ WAITNGLSMY PPKFEENPSN ASVSPVTIYP TPIPRKCFDE 70 80 90 100 110 120 AVQIQPVFNE LYARITQDMA QPDSYLHKTT EALALSDSEF TGKLWSLYLA TLKSAQYKKQ 130 140 150 160 170 180 NFRLGIFRSD YLIDKKKGTE QIKQVEFNTV SVSFAGLSEK VDRLHSYLNR ANKYDPKGPI 190 200 210 220 230 240 YNDQNMVISD SGYLLSKALA KAVESYKSQQ SSSTTSDPIV AFIVQRNERN VFDQKVLELN 250 260 270 280 290 300 LLEKFGTKSV RLTFDDVNDK LFIDDKTGKL FIRDTEQEIA VVYYRTGYTT TDYTSEKDWE 310 320 330 340 350 360 ARLFLEKSFA IKAPDLLTQL SGSKKIQQLL TDEGVLGKYI SDAEKKSSLL KTFVKIYPLD 370 380 390 400 410 420 DTKLGREGKR LALSEPSKYV LKPQREGGGN NVYKENIPNF LKGIEERHWD AYILMELIEP 430 440 450 460 470 480 ELNENNIILR DNKSYNEPII SELGIYGCVL FNDEQVLSNE FSGSLLRSKF NTSNEGGVAA 490 GFGCLDSIIL Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular identification of glutathione synthetase (GSH2) gene from Saccharomyces cerevisiae." Inoue Y., Sugiyama K.I., Izawa S., Kimura A. Biochim. Biophys. Acta 1395:315-320(1998) [PubMed: 9512666] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42, MASS SPECTROMETRY.
[6]	"Large conformational changes in the catalytic cycle of glutathione synthase." Gogos A., Shapiro L. Structure 10:1669-1676(2002) [PubMed: 12467574] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Y13804 Genomic DNA. Translation: CAA74136.1.
Z74791 Genomic DNA. Translation: CAA99054.1.
AY723864 Genomic DNA. Translation: AAU09781.1.

PIR

S66734.

RefSeq

NP_014593.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M0T	X-ray	2.30	A/B	1-491	[»]
1M0W	X-ray	1.80	A/B	1-491	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q08220. 2 interactions.

Proteomic databases

PeptideAtlas

Q08220.

Genome annotation databases

Ensembl

YOL049W. Saccharomyces cerevisiae. [Contig view]

GeneID

854108.

GenomeReviews

Gene locus YOL049W in contig Y13140_GR.

KEGG

sce:YOL049W.

NMPDR

fig|4932.3.peg.5685.

Organism-specific databases

CYGD

YOL049w.

SGD

S000005409. GSH2.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

Q08220.

OMA

Q08220. NATISQQ.

Enzyme and pathway databases

BRENDA

6.3.2.3. 250.

Gene expression databases

GermOnline

YOL049W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR013816. ATP_grasp_subdomain_2.
IPR004887. Glutathione_synth_subst-bd_euk.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
[Graphical view]

Gene3D

G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.1760. Glutathione_synth_subst-bd_euk. 1 hit.
G3DSA:3.30.1490.80. Glutathione_synthase_N_euk. 1 hit.

PANTHER

PTHR11130. GSH_synth_ATP_bd. 1 hit.

Pfam

PF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]

PIRSF

PIRSF001558. GSHase. 1 hit.

TIGRFAMs

TIGR01986. glut_syn_euk. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

975792.

Entry information

Entry name

GSHB_YEAST

Accession

Primary (citable) accession number: Q08220

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)