Reviewed, UniProtKB/Swiss-Prot Q08217 (KOE5_YEAST)

Names and origin

Protein names

Recommended name:
    Probable serine/threonine-protein kinase YOL045W
    EC=2.7.11.1

Gene names

Ordered Locus Names:

YOL045W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Miscellaneous	Present with 2220 molecules/cell in log phase SD medium. Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hexose metabolic process Inferred from genetic interaction. Source: SGD protein amino acid phosphorylation Inferred from direct assay. Source: SGD regulation of 1,6-beta-glucan biosynthetic process Inferred from genetic interaction. Source: SGD signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from direct assay. Source: SGD signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-9839,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1101	1101	Probable serine/threonine-protein kinase YOL045W		PRO_0000086156
Regions
	Domain	841 – 1099	259	Protein kinase
	Nucleotide binding	847 – 855	9	ATP By similarity
Sites
	Active site	975	1	Proton acceptor By similarity
	Binding site	870	1	ATP By similarity
Amino acid modifications
	Modified residue	118	1	Phosphothreonine Ref.4
	Modified residue	155	1	Phosphothreonine Ref.4
	Modified residue	156	1	Phosphoserine Ref.4
	Modified residue	434	1	Phosphoserine Ref.3
	Modified residue	680	1	Phosphoserine Ref.4
	Modified residue	692	1	Phosphoserine Ref.4
	Modified residue	717	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q08217-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3FC9CF3EF99B7937	FASTA	1,101	124,373
	10 20 30 40 50 60 MTYPVSAAAP ADISYSKNTP LVGLSKPPCL YQHASSSVDS FSSTFSDDDR SDLVAVPNES 70 80 90 100 110 120 PHAFSYNPIS PNSLGVRLTI LRRSLEIMVN SPDILHELKK KAPVIAYPPS LRHTRNLTET 130 140 150 160 170 180 ATLSASRDPL NGSLISPLVS NMPSPASRPV IQRATSLMVL PDNDTASKLN PAKSELENLL 190 200 210 220 230 240 FLLNLALENN SFERASDLHM LSLLNIKKIN FDSDIQKSET LKKVLLDSLA EPFFENYKKF 250 260 270 280 290 300 PHKDLGSKSQ YNEYEEKHDD IVSLADIKPQ QDYSRILHPF TSAKNSGPEA IFTCSQQYPW 310 320 330 340 350 360 NFKAANDLAC LTFGISKNVI KALTLLDLIH TDSRNFVLEK IMNAEDDNQE IVFTGETIPI 370 380 390 400 410 420 VQPNSTSNNN VPNLIWASLW AKRKNGLLVC VFEKTPCDYI DVMLNLRDFS VDSIIDTTHF 430 440 450 460 470 480 LENFDKKKQQ ESTSPMTEKK TVKFANEIHD IGSVSHSLSK LIDDVRFGKV FSADDDLLPL 490 500 510 520 530 540 SIRVANHVNE ERYFTLNCLS ENIPCAVTTS VLENEIKLKI HSLPYQAGLF IVDSHTLSLL 550 560 570 580 590 600 SFNKSVAKNM FGLRLHELAG SSVTKLVPSL ADMISYINKT YPMLNITLPE NKGLVLTEHF 610 620 630 640 650 660 FRKIEAEMHH DKDSFYTSIG LDGCHKDGNL IKVDVQLRVL NTNAVLLWIT HSRDVVIENY 670 680 690 700 710 720 TTVPSQLPML KENEIDVVGS RGSSSASSKK SSEKIPVNTL KAMADLSISS AETISNSDDE 730 740 750 760 770 780 VDLNQVNEKL RETSCGKVRG IESNDNNNYD DDMTMVDDPE LKHKIELTKM YTQDKSKFVK 790 800 810 820 830 840 DDNFKVDEKF IMRIIEPING EEIKKETNEL DKRNSTLKAT YLTTPEANIG SQKRIKKFSD 850 860 870 880 890 900 FTILQVMGEG AYGKVNLCIH NREHYIVVIK MIFKERILVD TWVRDRKLGT IPSEIQIMAT 910 920 930 940 950 960 LNKNSQENIL KLLDFFEDDD YYYIETPVHG ETGSIDLFDV IEFKKDMVEH EAKLVFKQVV 970 980 990 1000 1010 1020 ASIKHLHDQG IVHRDIKDEN VIVDSHGFVK LIDFGSAAYI KSGPFDVFVG TMDYAAPEVL 1030 1040 1050 1060 1070 1080 GGSSYKGKPQ DIWALGVLLY TIIYKENPYY NIDEILEGEL RFDKSEHVSE ECISLIKRIL 1090 1100 TREVDKRPTI DEIYEDKWLK I

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[3]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-434, MASS SPECTROMETRY.
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-118; THR-155; SER-156; SER-680; SER-692 AND SER-717, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z74788 Genomic DNA. Translation: CAA99051.1. Z74786 Genomic DNA. Translation: CAA99047.1.
PIR	S66730.
RefSeq	NP_014597.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6430N.
IntAct	Q08217. 4 interactions.
STRING	Q08217.
Proteomic databases
PeptideAtlas	Q08217.
PRIDE	Q08217.
Genome annotation databases
Ensembl	YOL045W; YOL045W; YOL045W; Saccharomyces cerevisiae. [Genome view]
GeneID	854111.
KEGG	sce:YOL045W.
NMPDR	fig|4932.3.peg.5689.
Organism-specific databases
CYGD	YOL045w.
SGD	S000005405. PSK2.
Phylogenomic databases
HOGENOM	Q08217.
OMA	TVKFANE
OrthoDB	EOG9NS4V7
Enzyme and pathway databases
BRENDA	2.7.11.1. 250.
Gene expression databases
ArrayExpress	Q08217.
Genevestigator	Q08217.
GermOnline	YOL045W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000014. PAS. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00091. PAS. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	975801.

Entry information

Entry name

KOE5_YEAST

Accession

Primary (citable) accession number: Q08217

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)