Q08214 (NTG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA base excision repair N-glycosylase 2

Gene names

Name:	NTG2
Ordered Locus Names:	YOL043C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Excision repair N-glycosylase involved in the repair of DNA base damage in the nucleus. NTG2 can use dihydrothymine (poor substrate), urea, and uracil glycol are substrates.
Subcellular location	Nucleus.
Miscellaneous	Present with 125 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the Nth/MutY family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	base-excision repair, AP site formation Inferred from direct assay PubMed 9826748. Source: SGD
Cellular_component	nucleus Inferred from direct assay Ref.3PubMed 19029246. Source: SGD
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity Inferred from direct assay PubMed 9020769. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	DNA base excision repair N-glycosylase 2		PRO_0000102238
Sites
	Metal binding	319	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	326	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	329	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	335	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08214 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 517F81C3BA3DDCF7	FASTA	380	43,844
	10 20 30 40 50 60 MREESRSRKR KHIPVDIEEV EVRSKYFKKN ERTVELVKEN KINKDLQNYG GVNIDWIKAL 70 80 90 100 110 120 KPIEYFEWIE SRTCDDPRTW GRPITKEEMI NDSGAKVPES FLPIYNRVRL MRSKVKTPVD 130 140 150 160 170 180 AMGCSMIPVL VSNKCGIPSE KVDPKNFRLQ FLIGTMLSAQ TRDERMAQAA LNITEYCLNT 190 200 210 220 230 240 LKIAEGITLD GLLKIDEPVL ANLIRCVSFY TRKANFIKRT AQLLVDNFDS DIPYDIEGIL 250 260 270 280 290 300 SLPGVGPKMG YLTLQKGWGL IAGICVDVHV HRLCKMWNWV DPIKCKTAEH TRKELQVWLP 310 320 330 340 350 360 HSLWYEINTV LVGFGQLICM ARGKRCDLCL ANDVCNARNE KLIESSKFHQ LEDKEDIEKV 370 380 YSHWLDTVTN GITTERHKKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Saccharomyces cerevisiae Ntg1p and Ntg2p: broad specificity N-glycosylases for the repair of oxidative DNA damage in the nucleus and mitochondria." You H.J., Swanson R.L., Harrington C., Corbett A.H., Jinks-Robertson S., Sentuerker S., Wallace S.S., Boiteux S., Dizdaroglu M., Doetsch P.W. Biochemistry 38:11298-11306(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74785 Genomic DNA. Translation: CAA99045.1. BK006948 Genomic DNA. Translation: DAA10739.1.
PIR	S66728.
RefSeq	NP_014599.1. NM_001183297.1.
3D structure databases
ProteinModelPortal	Q08214.
SMR	Q08214. Positions 204-342.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2956N.
IntAct	Q08214. 1 interaction.
MINT	MINT-570318.
STRING	4932.YOL043C.
Proteomic databases
PaxDb	Q08214.
PeptideAtlas	Q08214.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOL043C; YOL043C; YOL043C.
GeneID	854114.
KEGG	sce:YOL043C.
Organism-specific databases
CYGD	YOL043c.
SGD	S000005403. NTG2.
Phylogenomic databases
eggNOG	COG0177.
GeneTree	ENSGT00510000047513.
HOGENOM	HOG000252209.
KO	K10773.
OMA	CDLCLAN.
OrthoDB	EOG4T1MWS.
Gene expression databases
Genevestigator	Q08214.
GermOnline	YOL043C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.1670.10. 1 hit. 1.10.340.30. 1 hit.
InterPro	IPR011257. DNA_glycosylase. IPR004036. Endonuclease-III_CS2. IPR003651. Endouclease3_FeS-loop_motif. IPR003265. HhH-GPD_domain. IPR023170. HTH_base_excis_C. [Graphical view]
Pfam	PF00730. HhH-GPD. 1 hit. [Graphical view]
SMART	SM00478. ENDO3c. 1 hit. SM00525. FES. 1 hit. [Graphical view]
SUPFAM	SSF48150. DNA_glycsylse. 1 hit.
PROSITE	PS00764. ENDONUCLEASE_III_1. False negative. PS01155. ENDONUCLEASE_III_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975807.

Entry information

Entry name

NTG2_YEAST

Accession

Primary (citable) accession number: Q08214
Secondary accession number(s): D6W223

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program