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Protein

Nucleolar protein 12

Gene

NOP12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-25S rRNA processing.1 Publication

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • RNA binding Source: SGD
  • rRNA binding Source: GO_Central

GO - Biological processi

  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33455-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 12
Gene namesi
Name:NOP12
Ordered Locus Names:YOL041C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL041C.
SGDiS000005401. NOP12.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
  • preribosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 459459Nucleolar protein 12PRO_0000081675Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei70 – 701PhosphoserineCombined sources
Modified residuei181 – 1811PhosphothreonineCombined sources
Modified residuei184 – 1841PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08208.

PTM databases

iPTMnetiQ08208.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LOC1P435863EBI-35895,EBI-22906
MAK5P381124EBI-35895,EBI-10394
NOC2P397443EBI-35895,EBI-29259
NOP4P378385EBI-35895,EBI-12122
PRP43P531312EBI-35895,EBI-505

Protein-protein interaction databases

BioGridi34361. 207 interactions.
DIPiDIP-6505N.
IntActiQ08208. 42 interactions.
MINTiMINT-8285440.

Structurei

3D structure databases

ProteinModelPortaliQ08208.
SMRiQ08208. Positions 279-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini279 – 37193RRMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RRM RBM34 family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000011249.
HOGENOMiHOG000157415.
InParanoidiQ08208.
KOiK14837.
OMAiTKRSIAF.
OrthoDBiEOG7S7SQS.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08208-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSAIDNLFG NIDEKKIESS VDKLFSSSCG PINKLEVKSK TRTVLPDSKK
60 70 80 90 100
RERAAEADQE EKEASKPDVS DEQTEEVALP KVKKAKKSKR NDEDEDLEAR
110 120 130 140 150
YYAKLLNEEA EAEDDKPTVT KTDETSVPLT SAAKKVDFKE DELEKAERTV
160 170 180 190 200
FIGNILSTVI TSKKVYKEFK KLFGTNPIAE TEESGNEKEE ESSKKSDNNE
210 220 230 240 250
FAIESIRFRS ISFDEALPRK VAFVQQKFHK SRDTINAYIV YKNKSAVRKI
260 270 280 290 300
CSNLNAVVFQ DHHLRVDSVA HPAPHDKKRS IFVGNLDFEE IEESLWKHFE
310 320 330 340 350
PCGDIEYVRI IRDSKTNMGK GFAYVQFKDL QSVNKALLLN EKPMKSQKQE
360 370 380 390 400
DENTKKPTKK ARKLRVSRCK NMKKGTTIGT GLDRNGLTDS QRTRAGRAKK
410 420 430 440 450
ILGKADRATL GQEITIEGLR AKKGEGSTHL KKKKQRSATG RVTKRSIAFK

KAQAEKSKK
Length:459
Mass (Da):51,942
Last modified:November 1, 1996 - v1
Checksum:i338F73B13DAE3282
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74783 Genomic DNA. Translation: CAA99043.1.
BK006948 Genomic DNA. Translation: DAA10741.1.
PIRiS66726.
RefSeqiNP_014601.1. NM_001183295.1.

Genome annotation databases

EnsemblFungiiYOL041C; YOL041C; YOL041C.
GeneIDi854116.
KEGGisce:YOL041C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74783 Genomic DNA. Translation: CAA99043.1.
BK006948 Genomic DNA. Translation: DAA10741.1.
PIRiS66726.
RefSeqiNP_014601.1. NM_001183295.1.

3D structure databases

ProteinModelPortaliQ08208.
SMRiQ08208. Positions 279-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34361. 207 interactions.
DIPiDIP-6505N.
IntActiQ08208. 42 interactions.
MINTiMINT-8285440.

PTM databases

iPTMnetiQ08208.

Proteomic databases

MaxQBiQ08208.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL041C; YOL041C; YOL041C.
GeneIDi854116.
KEGGisce:YOL041C.

Organism-specific databases

EuPathDBiFungiDB:YOL041C.
SGDiS000005401. NOP12.

Phylogenomic databases

GeneTreeiENSGT00390000011249.
HOGENOMiHOG000157415.
InParanoidiQ08208.
KOiK14837.
OMAiTKRSIAF.
OrthoDBiEOG7S7SQS.

Enzyme and pathway databases

BioCyciYEAST:G3O-33455-MONOMER.

Miscellaneous databases

PROiQ08208.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Nucleolar protein Nop12p participates in synthesis of 25S rRNA in Saccharomyces cerevisiae."
    Wu K., Wu P., Aris J.P.
    Nucleic Acids Res. 29:2938-2949(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70 AND SER-184, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70 AND SER-184, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70; THR-181 AND SER-184, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNOP12_YEAST
AccessioniPrimary (citable) accession number: Q08208
Secondary accession number(s): D6W225
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5910 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.