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Protein

Phosphatidylcholine translocator ABCB4

Gene

Abcb4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts. Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes. Required for proper phospholipid bile formation. Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner. May promote biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane. In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts. Does not confer multidrug resistance.By similarity

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Enzyme regulationi

Translocation activity is inhibited by the ATPase inhibitor vanadate and the calcium channel blocker verapamil. Translocation activity is enhanced by the addition of the bile salt taurocholate.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi426 – 433ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1068 – 1075ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid transport, Transport
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylcholine translocator ABCB4
Alternative name(s):
ATP-binding cassette sub-family B member 4Imported
Multidrug resistance protein 21 Publication
Multidrug resistance protein 3By similarity (EC:3.6.3.44)
P-glycoprotein 21 Publication
P-glycoprotein 31 Publication
Gene namesi
Name:Abcb4Imported
Synonyms:Mdr21 Publication, Pgp31 Publication, Pgy21 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620248 Abcb4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 47CytoplasmicBy similarityAdd BLAST47
Transmembranei48 – 70HelicalPROSITE-ProRule annotationAdd BLAST23
Topological domaini71 – 115ExtracellularBy similarityAdd BLAST45
Transmembranei116 – 136HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini137 – 185CytoplasmicBy similarityAdd BLAST49
Transmembranei186 – 207HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini208 – 214ExtracellularBy similarity7
Transmembranei215 – 235HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini236 – 293CytoplasmicBy similarityAdd BLAST58
Transmembranei294 – 315HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini316 – 329ExtracellularBy similarityAdd BLAST14
Transmembranei330 – 351HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini352 – 710CytoplasmicBy similarityAdd BLAST359
Transmembranei711 – 731HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini732 – 754ExtracellularBy similarityAdd BLAST23
Transmembranei755 – 775HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini776 – 830CytoplasmicBy similarityAdd BLAST55
Transmembranei831 – 851HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini852ExtracellularBy similarity1
Transmembranei853 – 872HelicalPROSITE-ProRule annotationAdd BLAST20
Topological domaini873 – 932CytoplasmicBy similarityAdd BLAST60
Transmembranei933 – 955HelicalPROSITE-ProRule annotationAdd BLAST23
Topological domaini956 – 971ExtracellularBy similarityAdd BLAST16
Transmembranei972 – 993HelicalPROSITE-ProRule annotationAdd BLAST22
Topological domaini994 – 1278CytoplasmicBy similarityAdd BLAST285

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2073706

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933381 – 1278Phosphatidylcholine translocator ABCB4Add BLAST1278

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineBy similarity1
Glycosylationi88N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi94N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylated. Phosphorylation is required for PC efflux activity. Phosphorylation occurs on serine and threonine residues in a protein kinase A- or C-dependent manner. May be phosphorylated on Thr-41 and Ser-46.By similarity
Glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ08201
PRIDEiQ08201

PTM databases

PhosphoSitePlusiQ08201

Expressioni

Tissue specificityi

Expressed in the liver (PubMed:15159385). Expressed in hepatocytes (PubMed:7948020, PubMed:10067174).3 Publications

Interactioni

Subunit structurei

Interacts with HAX1 (PubMed:15159385). May interact with RACK1 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Hax1Q7TSE93EBI-929988,EBI-930005

Protein-protein interaction databases

IntActiQ08201, 1 interactor
STRINGi10116.ENSRNOP00000042556

Structurei

3D structure databases

ProteinModelPortaliQ08201
SMRiQ08201
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 356ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST303
Domaini391 – 627ABC transporter 1PROSITE-ProRule annotationAdd BLAST237
Domaini710 – 998ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST289
Domaini1033 – 1271ABC transporter 2PROSITE-ProRule annotationAdd BLAST239

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni622 – 646Interaction with HAX11 PublicationAdd BLAST25

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0055 Eukaryota
COG1132 LUCA
HOVERGENiHBG080809
InParanoidiQ08201
KOiK05659
PhylomeDBiQ08201

Family and domain databases

Gene3Di1.20.1560.10, 2 hits
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011527 ABC1_TM_dom
IPR036640 ABC1_TM_sf
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR030275 MDR3
IPR027417 P-loop_NTPase
PANTHERiPTHR24221:SF241 PTHR24221:SF241, 2 hits
PfamiView protein in Pfam
PF00664 ABC_membrane, 2 hits
PF00005 ABC_tran, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SUPFAMiSSF52540 SSF52540, 2 hits
SSF90123 SSF90123, 2 hits
PROSITEiView protein in PROSITE
PS50929 ABC_TM1F, 2 hits
PS00211 ABC_TRANSPORTER_1, 2 hits
PS50893 ABC_TRANSPORTER_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q08201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLEAARNGT ARRLDGDFEL GSISNQSREK KKKVNLIGPL TLFRYSDWQD
60 70 80 90 100
KLFMLLGTAM AIAHGSGLPL MMIVFGEMTD KFVDNAGNFS LPVNFSLSML
110 120 130 140 150
NPGRILEEEM TRYAYYYSGL GGGVLLAAYI QVSFWTLAAG RQIRKIRQKF
160 170 180 190 200
FHAILRQEMG WFDIKGTTEL NTRLTDDISK ISEGIGDKVG MFFQAIATFF
210 220 230 240 250
AGFIVGFIRG WKLTLVIMAI TAILGLSTAV WAKILSTFSD KELAAYAKAG
260 270 280 290 300
AVAEEALGAI RTVIAFGGQN KELERYQKHL ENAKKIGIKK AISANISMGI
310 320 330 340 350
AFLLIYASYA LAFWYGSTLV ISKEYTIGNA MTVFFSILIG AFSVGQAAPC
360 370 380 390 400
IDAFPNARGA AYVIFDIIDN NPKIDSFSER GHKPDSIKGN LEFSDVHFSY
410 420 430 440 450
PSRANIKILK GLNLKVKSGQ TVALVGNSGC GKSTTVQLLQ RLYDPTEGTI
460 470 480 490 500
SIDGQDIRNF NVRCLREFIG VVSQEPVLFS TTIAENIRYG RGNVTMDEIK
510 520 530 540 550
KAVKEANAYD FIMKLPQKFD TLVGDRGAQL SGGQKQRIAI ARALVRNPKI
560 570 580 590 600
LLLDEATSAL DTESEAEVQA ALDKAREGRT TIVIAHRLST VRNADVIAGF
610 620 630 640 650
EDGVIVEQGS HSELIKKEGI YFRLVNMQTS GSQILSEEFE VELSDEKAAG
660 670 680 690 700
GVAPNGWKAR IFRNSTKKSL KSSRAHQNRL DVETNELDAN VPPVSFLKVL
710 720 730 740 750
RLNKTEWPYF VVGTLCAIAN GALQPAFSII LSEMIAIFGP GDDTVKQQKC
760 770 780 790 800
NMFSLVFLGL GVHSFFTFFL QGFTFGKAGE ILTTRLRSMA FKAMLRQDMS
810 820 830 840 850
WFDDHKNSTG ALSTRLATDA AQVQGATGTR LALIAQNTAN LGTGIIISFI
860 870 880 890 900
YGWQLTLLLL SVVPFIAVAG IVEMKMLAGN AKRDKKEMEA AGKIATEAIE
910 920 930 940 950
NIRTVVSLTQ ERKFESMYVE KLHGPYRNSV RKAHIYGITF SISQAFMYFS
960 970 980 990 1000
YAGCFRFGSY LIVNGHMRFK DVILVFSAIV LGAVALGHAS SFAPDYAKAK
1010 1020 1030 1040 1050
LSAAYLFSLF ERQPLIDSYS REGMWPDKFE GSVTFNEVVF NYPTRANVPV
1060 1070 1080 1090 1100
LQGLSLEVKK GQTLALVGSS GCGKSTVVQL LERFYDPMAG TVLLDGQEAK
1110 1120 1130 1140 1150
KLNVQWLRAQ LGIVSQEPIL FDCSIAKNIA YGDNSRVVSQ DEIVRAAKEA
1160 1170 1180 1190 1200
NIHPFIETLP QKYETRVGDK GTQLSGGQKQ RIAIARALIR QPRVLLLDEA
1210 1220 1230 1240 1250
TSALDTESEK VVQEALDKAR EGRTCIVIAH RLSTIQNADL IVVIDNGKVK
1260 1270
EHGTHQQLLA QKGIYFSMVN IQAGTQNL
Length:1,278
Mass (Da):140,655
Last modified:November 1, 1995 - v1
Checksum:iD4FB6BE745AF73BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15079 mRNA Translation: AAA02937.1
X61105 Genomic DNA Translation: CAA43417.1
PIRiS41646
RefSeqiNP_036822.1, NM_012690.2
UniGeneiRn.82691

Genome annotation databases

GeneIDi24891
KEGGirno:24891

Similar proteinsi

Entry informationi

Entry nameiMDR3_RAT
AccessioniPrimary (citable) accession number: Q08201
Secondary accession number(s): Q78E07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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