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Q081T9 (Q081T9_SHEFN) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
DNA gyrase subunit A HAMAP-Rule MF_01897
EC=5.99.1.3 HAMAP-Rule MF_01897
Gene names
Name:gyrA HAMAP-Rule MF_01897
Ordered Locus Names:Sfri_2130
OrganismShewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP] EMBL ABI71976.1
Taxonomic identifier318167 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella ›

Protein attributes

Sequence length909 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897 SAAS SAAS005743

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01897.

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1221O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

Sequence LengthMass (Da)Tools
Q081T9 [UniParc].

Last modified October 31, 2006. Version 1.
Checksum: 58FD2B28B7C3FD82

FASTA909100,463
        10         20         30         40         50         60 
MTDLASSISP INIEDELKNS YLDYAMSVIV GRALPDVRDG LKPVHRRVLF AMSELKNDWN 

        70         80         90        100        110        120 
KPYKKSARVV GDVIGKYHPH GDTAVYDTIV RMAQPFSLRY TLIDGQGNFG SVDGDSAAAM 

       130        140        150        160        170        180 
RYTEIRMQKL AHSLLADLEK ETVDFVPNYD GTEMIPAVLP TRVPNLLING SSGIAVGMAT 

       190        200        210        220        230        240 
NIPPHNLTEV VKGCLALIED PSLSIEQLME YIPGPDFPTA ASINGRKGII DAYNTGRGRA 

       250        260        270        280        290        300 
IMRSKAEIET EENGRERIIV HEIPYQVNKA RLIEKIAELV KDKKLEGISG LRDESDKDGM 

       310        320        330        340        350        360 
RIVIEIKRGE VGEVVLNNLY SQTQMQCSFG INMVALTNGQ PKLFNLKEML ECFILHRREV 

       370        380        390        400        410        420 
VTRRTVFELR KARERAHVLE ALAIALANID PIIALIKASP TPAEAKAKLV AQGWELGHVQ 

       430        440        450        460        470        480 
GMLEKAGDDA ARPEWLEPEF GIRDGQYYLT EQQAQAILEL RLHRLTGLEH DKILAEYEEL 

       490        500        510        520        530        540 
LVLIAGLLFI LRTPSRLMEV IKEELEEILE QYGDERRTII NANEIDMSLE DLINEEDVVV 

       550        560        570        580        590        600 
TLSHLGYAKY QVLSDYQAQR RGGKGKAATK VKDEDFVEKL LVANTHDTIL CFSDFGKMYW 

       610        620        630        640        650        660 
LKVYQLPLAS RTARGRPIVN LLPLSDGEHI TAILPVREYA DDKYIIMATA HGTVKKTALT 

       670        680        690        700        710        720 
AYSNPRANGI IAVNLKDGDQ LIGVDITEGS DDIMLFSNEG KVVRFNEKAR DSETGEVKID 

       730        740        750        760        770        780 
AETGEEIIAL RPMGRTATGV RGIKLDAGQK VVSLIVPKGD GAILTVTENG YGKRTELSEY 

       790        800        810        820        830        840 
PAKSRGTKGV VSIKVSERNG EVVGAVQVGT FDEIMLISNK GTLVRTPAEG VSIIGRNTQG 

       850        860        870        880        890        900 
VTIIRTAEDE KVVGLQRIEE IQTEELLDEE GNVIPVSDVI DAEVTDAEST DDVEATDPDE 


AKTGDDEQE

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References

[1]"Complete sequence of Shewanella frigidimarina NCIMB 400."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Fredrickson J.K., Kolker E., McCuel L.A., DiChristina T., Nealson K.H., Newman D., Tiedje J.M. expand/collapse author list , Zhou J., Romine M.F., Culley D.E., Serres M., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCIMB 400.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000447 Genomic DNA. Translation: ABI71976.1.
RefSeqYP_750814.1. NC_008345.1.

3D structure databases

ProteinModelPortalQ081T9.
SMRQ081T9. Positions 30-520, 535-860.
ModBaseSearch...

Protein-protein interaction databases

STRING318167.Sfri_2130.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI71976; ABI71976; Sfri_2130.
GeneID4279042.
KEGGsfr:Sfri_2130.
PATRIC23498265. VBISheFri14343_2211.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0188.
HOGENOMHOG000076278.
KOK02469.
OMAIAQEDVV.
ProtClustDBPRK05560.

Enzyme and pathway databases

BioCycSFRI318167:GIXS-2204-MONOMER.

Family and domain databases

Gene3D1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.90.199.10. 1 hit.
HAMAPMF_01897. GyrA.
InterProIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMsTIGR01063. gyrA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQ081T9_SHEFN
AccessionPrimary (citable) accession number: Q081T9
Entry history
Integrated into UniProtKB/TrEMBL: October 31, 2006
Last sequence update: October 31, 2006
Last modified: May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info