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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Shewanella frigidimarina (strain NCIMB 400)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathway: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei415 – 4151NucleophileUniRule annotation
Active sitei468 – 4681Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciSFRI318167:GIXS-2240-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Sfri_2165
OrganismiShewanella frigidimarina (strain NCIMB 400)
Taxonomic identifieri318167 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000000684 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7447441,4-alpha-glucan branching enzyme GlgBPRO_0000260698Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi318167.Sfri_2165.

Structurei

3D structure databases

ProteinModelPortaliQ081Q4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q081Q4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVATPYFQQ GSEIALLNGE YTDVFSLLGM HSVNDGKALV VRCLIPGAIS
60 70 80 90 100
VDVLSAKDGR KVATLEQVNE HGLFAGKMGR RVKSFAYLLR VQYPLCEQLI
110 120 130 140 150
NDPYQFDSLL NPDDVYLFGE GSQLQTYHFQ GANWREHHGV KGVHFCVWAP
160 170 180 190 200
NAKQVAVMGD FNLWDNKRHI LRHHPASGLW DIFIAEVEPE QHYKYAISDM
210 220 230 240 250
HGNQVIKSDP YAVAMQPSPH NASKIPHVER YDWQDSQWLA DRASHQPHVQ
260 270 280 290 300
PMSIYEVQLA SWRRKGDDSQ EYTDYSQLIA ELVPYIKEMG FTHLQLMPIS
310 320 330 340 350
EYPFDGSWGY QPVGLFAPTY RFGDANGLRA FIDECHQQGI AVLLDWVPAH
360 370 380 390 400
FPRDPHGLVR FDGSCLYEHD DPRKGEQPDW DTLIYNYGRA EVRSFLYSNA
410 420 430 440 450
HYWLDEFHFD GLRLDAVSSM LYLDYSRRAD QWIPNKFGGR ENLEAISFLQ
460 470 480 490 500
ELNARMYQCF PGINMIAEES TAWPGVTQAT SNNGLGFGFK WNMGWMNDTL
510 520 530 540 550
RYISCDPLFR RYHHGELTFS LVYAFTEQFI LSLSHDEVVH GKGSLLHKIP
560 570 580 590 600
GDDWQKFATL RAYYGFMWTH PGKKLLFMGN EFAQRNEWNH NQSLDWHLLT
610 620 630 640 650
YAPHQGVQDW VRDLNQCYQQ YPALYQRDHH SDGFQWLDCN NADNNILVFC
660 670 680 690 700
RFGMDKQQHV VIVVNMSPQV YYDFRVGVPT AQNYRELLNS DHRHYGGGDV
710 720 730 740
VNDNPCEAQV TPWQGMSHSI VITVPPLGCS IWVPELDEQD QPTQ
Length:744
Mass (Da):85,845
Last modified:October 31, 2006 - v1
Checksum:iC6F3490C64FA7E99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000447 Genomic DNA. Translation: ABI72011.1.
RefSeqiWP_011637621.1. NC_008345.1.
YP_750849.1. NC_008345.1.

Genome annotation databases

EnsemblBacteriaiABI72011; ABI72011; Sfri_2165.
KEGGisfr:Sfri_2165.
PATRICi23498343. VBISheFri14343_2249.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000447 Genomic DNA. Translation: ABI72011.1.
RefSeqiWP_011637621.1. NC_008345.1.
YP_750849.1. NC_008345.1.

3D structure databases

ProteinModelPortaliQ081Q4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi318167.Sfri_2165.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI72011; ABI72011; Sfri_2165.
KEGGisfr:Sfri_2165.
PATRICi23498343. VBISheFri14343_2249.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciSFRI318167:GIXS-2240-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCIMB 400.

Entry informationi

Entry nameiGLGB_SHEFN
AccessioniPrimary (citable) accession number: Q081Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 31, 2006
Last modified: May 27, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.