Q08199 (SIL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleotide exchange factor SIL1

Alternative name(s):
Protein SLS1

Gene names

Name:	SIL1
Synonyms:	PER100, SLS1
Ordered Locus Names:	YOL031C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone KAR2. Ref.5 Ref.6 Ref.8
Subunit structure	Interacts with KAR2. Ref.5 Ref.6 Ref.8
Subcellular location	Endoplasmic reticulum lumen.
Induction	By the unfolded protein response (UPR). Ref.4
Post-translational modification	N-glycosylated.
Miscellaneous	Present with 2420 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SIL1 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Endoplasmic reticulum
Domain	Signal
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane, translocation Inferred from genetic interaction Ref.6. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from physical interaction Ref.6. Source: SGD endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	adenyl-nucleotide exchange factor activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
KAR2	P16474	1	EBI-38850,EBI-7876

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 421

402

Nucleotide exchange factor SIL1

PRO_0000223364

Regions

Motif

418 – 421

4

Prevents secretion from ER Potential

Amino acid modifications

Glycosylation

105

1

N-linked (GlcNAc...) Potential

Glycosylation

181

1

N-linked (GlcNAc...) Potential

Glycosylation

215

1

N-linked (GlcNAc...) Potential

Glycosylation

233

1

N-linked (GlcNAc...) Potential

Glycosylation

315

1

N-linked (GlcNAc...) Potential

Glycosylation

333

1

N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis

365 – 369

5

Missing: Abrogates interaction with KAR2. Ref.6

Sequence conflict

51

1

I → T in AAT93120. Ref.3

Secondary structure

1

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421

Helix Strand Turn

Details...

Helix

127 – 139

13

Helix

145 – 158

14

Helix

159 – 161

3

Helix

165 – 184

20

Helix

190 – 204

15

Helix

208 – 217

10

Helix

221 – 233

13

Helix

241 – 253

13

Helix

266 – 275

10

Turn

276 – 278

3

Helix

280 – 297

18

Helix

321 – 331

11

Helix

338 – 355

18

Helix

356 – 358

3

Helix

363 – 380

18

Helix

389 – 402

14

Turn

403 – 405

3

Sequences

	Sequence		Length	Mass (Da)
	Q08199 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4F6677F5029B6209	FASTA	421	48,275
	10 20 30 40 50 60 MVRILPIILS ALSSKLVAST ILHSSIHSVP SGGEIISAED LKELEISGNS ICVDNRCYPK 70 80 90 100 110 120 IFEPRHDWQP ILPGQELPGG LDIRINMDTG LKEAKLNDEK NVGDNGSHEL IVSSEDMKAS 130 140 150 160 170 180 PGDYEFSSDF KEMRNIIDSN PTLSSQDIAR LEDSFDRIME FAHDYKHGYK IITHEFALLA 190 200 210 220 230 240 NLSLNENLPL TLRELSTRVI TSCLRNNPPV VEFINESFPN FKSKIMAALS NLNDSNHRSS 250 260 270 280 290 300 NILIKRYLSI LNELPVTSED LPIYSTVVLQ NVYERNNKDK QLQIKVLELI SKILKADMYE 310 320 330 340 350 360 NDDTNLILFK RNAENWSSNL QEWANEFQEM VQNKSIDELH TRTFFDTLYN LKKIFKSDIT 370 380 390 400 410 420 INKGFLNWLA QQCKARQSNL DNGLQERDTE QDSFDKKLID SRHLIFGNPM AHRIKNFRDE L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation." Travers K.J., Patil C.K., Wodicka L., Lockhart D.J., Weissman J.S., Walter P. Cell 101:249-258(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[5]	"LHS1 and SIL1 provide a lumenal function that is essential for protein translocation into the endoplasmic reticulum." Tyson J.R., Stirling C.J. EMBO J. 19:6440-6452(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH KAR2.
[6]	"Sls1p stimulates Sec63p-mediated activation of Kar2p in a conformation-dependent manner in the yeast endoplasmic reticulum." Kabani M., Beckerich J.-M., Gaillardin C. Mol. Cell. Biol. 20:6923-6934(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH KAR2, MUTAGENESIS OF 365-PHE--LEU-369.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Coordinated activation of Hsp70 chaperones." Steel G.J., Fullerton D.M., Tyson J.R., Stirling C.J. Science 303:98-101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH KAR2.
[9]	"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes." Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H. Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z74773 Genomic DNA. Translation: CAA99031.1.
AY693101 Genomic DNA. Translation: AAT93120.1.
BK006948 Genomic DNA. Translation: DAA10750.1.

PIR

S66714.

RefSeq

NP_014611.1. NM_001183285.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3QML	X-ray	2.31	C/D	113-421	[»]

ProteinModelPortal

Q08199.

SMR

Q08199. Positions 125-406.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2622N.

IntAct

Q08199. 6 interactions.

MINT

MINT-558162.

STRING

4932.YOL031C.

Proteomic databases

PaxDb

Q08199.

PeptideAtlas

Q08199.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOL031C; YOL031C; YOL031C.

GeneID

854126.

KEGG

sce:YOL031C.

Organism-specific databases

CYGD

YOL031c.

SGD

S000005391. SIL1.

Phylogenomic databases

eggNOG

NOG244873.

HOGENOM

HOG000154325.

KO

K14001.

OMA

SHDIEFG.

OrthoDB

EOG40312K.

Gene expression databases

Genevestigator

Q08199.

GermOnline

YOL031C. Saccharomyces cerevisiae.

Family and domain databases

PROSITE

PS00014. ER_TARGET. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

975837.

Entry information

Entry name

SIL1_YEAST

Accession

Primary (citable) accession number: Q08199
Secondary accession number(s): D6W234, Q6B1H9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program