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Protein

Nucleotide exchange factor SIL1

Gene

SIL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone KAR2.3 Publications

Miscellaneous

Present with 2420 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • adenyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  • SRP-dependent cotranslational protein targeting to membrane, translocation Source: SGD

Keywordsi

Biological processProtein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33447-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleotide exchange factor SIL1
Alternative name(s):
Protein SLS1
Gene namesi
Name:SIL1
Synonyms:PER100, SLS1
Ordered Locus Names:YOL031C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL031C
SGDiS000005391 SIL1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi365 – 369Missing : Abrogates interaction with KAR2. 1 Publication5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000022336420 – 421Nucleotide exchange factor SIL1Add BLAST402

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi105N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi181N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi215N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi233N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi315N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi333N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ08199
PaxDbiQ08199
PRIDEiQ08199

PTM databases

iPTMnetiQ08199

Expressioni

Inductioni

By the unfolded protein response (UPR).1 Publication

Interactioni

Subunit structurei

Interacts with KAR2.3 Publications

Protein-protein interaction databases

BioGridi34369, 124 interactors
DIPiDIP-2622N
IntActiQ08199, 6 interactors
MINTiQ08199
STRINGi4932.YOL031C

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi127 – 139Combined sources13
Helixi145 – 158Combined sources14
Helixi159 – 161Combined sources3
Helixi165 – 184Combined sources20
Helixi190 – 204Combined sources15
Helixi208 – 217Combined sources10
Helixi221 – 233Combined sources13
Helixi241 – 253Combined sources13
Helixi266 – 275Combined sources10
Turni276 – 278Combined sources3
Helixi280 – 297Combined sources18
Helixi306 – 316Combined sources11
Helixi321 – 331Combined sources11
Helixi338 – 355Combined sources18
Helixi356 – 358Combined sources3
Helixi363 – 380Combined sources18
Helixi389 – 402Combined sources14
Turni403 – 405Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QMLX-ray2.31C/D113-421[»]
ProteinModelPortaliQ08199
SMRiQ08199
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi418 – 421Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the SIL1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000154325
InParanoidiQ08199
KOiK14001
OMAiSHDIEFG
OrthoDBiEOG092C3G73

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR031884 Sil1_fungi
PfamiView protein in Pfam
PF16782 SIL1, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRILPIILS ALSSKLVAST ILHSSIHSVP SGGEIISAED LKELEISGNS
60 70 80 90 100
ICVDNRCYPK IFEPRHDWQP ILPGQELPGG LDIRINMDTG LKEAKLNDEK
110 120 130 140 150
NVGDNGSHEL IVSSEDMKAS PGDYEFSSDF KEMRNIIDSN PTLSSQDIAR
160 170 180 190 200
LEDSFDRIME FAHDYKHGYK IITHEFALLA NLSLNENLPL TLRELSTRVI
210 220 230 240 250
TSCLRNNPPV VEFINESFPN FKSKIMAALS NLNDSNHRSS NILIKRYLSI
260 270 280 290 300
LNELPVTSED LPIYSTVVLQ NVYERNNKDK QLQIKVLELI SKILKADMYE
310 320 330 340 350
NDDTNLILFK RNAENWSSNL QEWANEFQEM VQNKSIDELH TRTFFDTLYN
360 370 380 390 400
LKKIFKSDIT INKGFLNWLA QQCKARQSNL DNGLQERDTE QDSFDKKLID
410 420
SRHLIFGNPM AHRIKNFRDE L
Length:421
Mass (Da):48,275
Last modified:November 1, 1996 - v1
Checksum:i4F6677F5029B6209
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51I → T in AAT93120 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74773 Genomic DNA Translation: CAA99031.1
AY693101 Genomic DNA Translation: AAT93120.1
BK006948 Genomic DNA Translation: DAA10750.1
PIRiS66714
RefSeqiNP_014611.1, NM_001183285.1

Genome annotation databases

EnsemblFungiiYOL031C; YOL031C; YOL031C
GeneIDi854126
KEGGisce:YOL031C

Similar proteinsi

Entry informationi

Entry nameiSIL1_YEAST
AccessioniPrimary (citable) accession number: Q08199
Secondary accession number(s): D6W234, Q6B1H9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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