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Protein

Nucleotide exchange factor SIL1

Gene

SIL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone KAR2.3 Publications

GO - Molecular functioni

  • adenyl-nucleotide exchange factor activity Source: SGD

GO - Biological processi

  • SRP-dependent cotranslational protein targeting to membrane, translocation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33447-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleotide exchange factor SIL1
Alternative name(s):
Protein SLS1
Gene namesi
Name:SIL1
Synonyms:PER100, SLS1
Ordered Locus Names:YOL031C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL031C.
SGDiS000005391. SIL1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi365 – 369Missing : Abrogates interaction with KAR2. 1 Publication5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000022336420 – 421Nucleotide exchange factor SIL1Add BLAST402

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi105N-linked (GlcNAc...)Sequence analysis1
Glycosylationi181N-linked (GlcNAc...)Sequence analysis1
Glycosylationi215N-linked (GlcNAc...)Sequence analysis1
Glycosylationi233N-linked (GlcNAc...)Sequence analysis1
Glycosylationi315N-linked (GlcNAc...)Sequence analysis1
Glycosylationi333N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ08199.
PRIDEiQ08199.

PTM databases

iPTMnetiQ08199.

Expressioni

Inductioni

By the unfolded protein response (UPR).1 Publication

Interactioni

Subunit structurei

Interacts with KAR2.3 Publications

Protein-protein interaction databases

BioGridi34369. 60 interactors.
DIPiDIP-2622N.
IntActiQ08199. 6 interactors.
MINTiMINT-558162.

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi127 – 139Combined sources13
Helixi145 – 158Combined sources14
Helixi159 – 161Combined sources3
Helixi165 – 184Combined sources20
Helixi190 – 204Combined sources15
Helixi208 – 217Combined sources10
Helixi221 – 233Combined sources13
Helixi241 – 253Combined sources13
Helixi266 – 275Combined sources10
Turni276 – 278Combined sources3
Helixi280 – 297Combined sources18
Helixi306 – 316Combined sources11
Helixi321 – 331Combined sources11
Helixi338 – 355Combined sources18
Helixi356 – 358Combined sources3
Helixi363 – 380Combined sources18
Helixi389 – 402Combined sources14
Turni403 – 405Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QMLX-ray2.31C/D113-421[»]
ProteinModelPortaliQ08199.
SMRiQ08199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi418 – 421Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the SIL1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000154325.
InParanoidiQ08199.
KOiK14001.
OMAiSHDIEFG.
OrthoDBiEOG092C3G73.

Family and domain databases

InterProiIPR031884. Sil1_fungi.
[Graphical view]
PfamiPF16782. SIL1. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRILPIILS ALSSKLVAST ILHSSIHSVP SGGEIISAED LKELEISGNS
60 70 80 90 100
ICVDNRCYPK IFEPRHDWQP ILPGQELPGG LDIRINMDTG LKEAKLNDEK
110 120 130 140 150
NVGDNGSHEL IVSSEDMKAS PGDYEFSSDF KEMRNIIDSN PTLSSQDIAR
160 170 180 190 200
LEDSFDRIME FAHDYKHGYK IITHEFALLA NLSLNENLPL TLRELSTRVI
210 220 230 240 250
TSCLRNNPPV VEFINESFPN FKSKIMAALS NLNDSNHRSS NILIKRYLSI
260 270 280 290 300
LNELPVTSED LPIYSTVVLQ NVYERNNKDK QLQIKVLELI SKILKADMYE
310 320 330 340 350
NDDTNLILFK RNAENWSSNL QEWANEFQEM VQNKSIDELH TRTFFDTLYN
360 370 380 390 400
LKKIFKSDIT INKGFLNWLA QQCKARQSNL DNGLQERDTE QDSFDKKLID
410 420
SRHLIFGNPM AHRIKNFRDE L
Length:421
Mass (Da):48,275
Last modified:November 1, 1996 - v1
Checksum:i4F6677F5029B6209
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51I → T in AAT93120 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74773 Genomic DNA. Translation: CAA99031.1.
AY693101 Genomic DNA. Translation: AAT93120.1.
BK006948 Genomic DNA. Translation: DAA10750.1.
PIRiS66714.
RefSeqiNP_014611.1. NM_001183285.1.

Genome annotation databases

EnsemblFungiiYOL031C; YOL031C; YOL031C.
GeneIDi854126.
KEGGisce:YOL031C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74773 Genomic DNA. Translation: CAA99031.1.
AY693101 Genomic DNA. Translation: AAT93120.1.
BK006948 Genomic DNA. Translation: DAA10750.1.
PIRiS66714.
RefSeqiNP_014611.1. NM_001183285.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QMLX-ray2.31C/D113-421[»]
ProteinModelPortaliQ08199.
SMRiQ08199.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34369. 60 interactors.
DIPiDIP-2622N.
IntActiQ08199. 6 interactors.
MINTiMINT-558162.

PTM databases

iPTMnetiQ08199.

Proteomic databases

MaxQBiQ08199.
PRIDEiQ08199.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL031C; YOL031C; YOL031C.
GeneIDi854126.
KEGGisce:YOL031C.

Organism-specific databases

EuPathDBiFungiDB:YOL031C.
SGDiS000005391. SIL1.

Phylogenomic databases

HOGENOMiHOG000154325.
InParanoidiQ08199.
KOiK14001.
OMAiSHDIEFG.
OrthoDBiEOG092C3G73.

Enzyme and pathway databases

BioCyciYEAST:G3O-33447-MONOMER.

Miscellaneous databases

PROiQ08199.

Family and domain databases

InterProiIPR031884. Sil1_fungi.
[Graphical view]
PfamiPF16782. SIL1. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIL1_YEAST
AccessioniPrimary (citable) accession number: Q08199
Secondary accession number(s): D6W234, Q6B1H9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2420 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.