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Protein

1,3-beta-glucanosyltransferase GAS5

Gene

GAS5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in cell wall biosynthesis and morphogenesis.1 Publication

Miscellaneous

Present with 11700 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89Donor substrate; via carbonyl oxygenBy similarity1
Binding sitei159Donor substrateBy similarity1
Active sitei160Proton donorBy similarity1
Binding sitei160Acceptor substrateBy similarity1
Binding sitei201Acceptor substrate; via carbonyl oxygenBy similarity1
Binding sitei206Acceptor substrateBy similarity1
Active sitei262NucleophileBy similarity1
Binding sitei295Donor substrateBy similarity1

GO - Molecular functioni

  • 1,3-beta-glucanosyltransferase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-33446-MONOMER.

Protein family/group databases

CAZyiGH72. Glycoside Hydrolase Family 72.

Names & Taxonomyi

Protein namesi
Recommended name:
1,3-beta-glucanosyltransferase GAS5 (EC:2.4.1.-)
Alternative name(s):
Glycolipid-anchored surface protein 5
Gene namesi
Name:GAS5
Ordered Locus Names:YOL030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL030W.
SGDiS000005390. GAS5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001048120 – 4621,3-beta-glucanosyltransferase GAS5Add BLAST443
PropeptideiPRO_0000010482463 – 484Removed in mature formSequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi71 ↔ 100By similarity
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi215 ↔ 348By similarity
Disulfide bondi234 ↔ 265By similarity
Glycosylationi299N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi344N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi359N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi462GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ08193.
PRIDEiQ08193.

Interactioni

Protein-protein interaction databases

BioGridi34370. 81 interactors.
DIPiDIP-4240N.
IntActiQ08193. 14 interactors.
MINTiMINT-545137.
STRINGi4932.YOL030W.

Structurei

3D structure databases

ProteinModelPortaliQ08193.
SMRiQ08193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi395 – 403Asp/Glu-rich (highly acidic)9
Compositional biasi405 – 460Ser-richAdd BLAST56
Compositional biasi405 – 427Poly-SerAdd BLAST23
Compositional biasi432 – 439Poly-Ser8

Sequence similaritiesi

Belongs to the glycosyl hydrolase 72 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000011003.
HOGENOMiHOG000164982.
InParanoidiQ08193.
OMAiAQYMNCG.
OrthoDBiEOG092C1TYR.

Family and domain databases

InterProiView protein in InterPro
IPR004886. Glucanosyltransferase.
IPR017853. Glycoside_hydrolase_SF.
PANTHERiPTHR31468. PTHR31468. 1 hit.
PfamiView protein in Pfam
PF03198. Glyco_hydro_72. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRSLTSAF VLSAGLAQAA SSSNSSTPSI EIKGNAFFNS ESGERFYIRG
60 70 80 90 100
VDYQPGGSSN LTDPLADASV CDRDVPVLKD LGINTVRVYT VDNSQDHSHC
110 120 130 140 150
MKLLQENGIY LILDVNTPTS AISRYDPACS YNADYLQNVF ATIDTFADYD
160 170 180 190 200
NVLGFFAGNE VINSVNTTNT ATYVKAVVRD MKKYIKARKY RQIPVGYSAA
210 220 230 240 250
DIVANRQLAA EYFNCGDEAD ARIDMFGVND YSWCGESSFV VSGYSTKMKL
260 270 280 290 300
YQDYSVPVFL SEFGCNQVKS SRPFTEIEAI YSTQMSSVFS GGLVYEYSNE
310 320 330 340 350
TNNYGLVQID GDKVTKLTDF ENLKNEYSKV SNPEGNGGYS TSNNYSTCPD
360 370 380 390 400
YEKGVWEANN TLPAMPSAAS AYFTSGAGSP MGTGIATQQS CDAKDDDDEE
410 420 430 440 450
DDDTSSSSSS SSSSSSSASS SSESSSSTSK ASSSSPSASE TSLLKSAASA
460 470 480
TSSSQSSSKS KGAAGIIEIP LIFRALAELY NLVL
Length:484
Mass (Da):51,870
Last modified:November 1, 1997 - v1
Checksum:iE65B9F4689B8B34C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74772 Genomic DNA. Translation: CAA99030.1.
AY693093 Genomic DNA. Translation: AAT93112.1.
BK006948 Genomic DNA. Translation: DAA10751.1.
PIRiS66713.
RefSeqiNP_014612.1. NM_001183284.1.

Genome annotation databases

EnsemblFungiiYOL030W; YOL030W; YOL030W.
GeneIDi854127.
KEGGisce:YOL030W.

Similar proteinsi

Entry informationi

Entry nameiGAS5_YEAST
AccessioniPrimary (citable) accession number: Q08193
Secondary accession number(s): D6W235
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 27, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names