Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q08193

- GAS5_YEAST

UniProt

Q08193 - GAS5_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

1,3-beta-glucanosyltransferase GAS5

Gene

GAS5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in cell wall biosynthesis and morphogenesis.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891Donor substrate; via carbonyl oxygenBy similarity
Binding sitei159 – 1591Donor substrateBy similarity
Active sitei160 – 1601Proton donorBy similarity
Binding sitei160 – 1601Acceptor substrateBy similarity
Binding sitei201 – 2011Acceptor substrate; via carbonyl oxygenBy similarity
Binding sitei206 – 2061Acceptor substrateBy similarity
Active sitei262 – 2621NucleophileBy similarity
Binding sitei295 – 2951Donor substrateBy similarity

GO - Molecular functioni

  1. 1,3-beta-glucanosyltransferase activity Source: SGD

GO - Biological processi

  1. (1->3)-beta-D-glucan metabolic process Source: SGD
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-33446-MONOMER.

Protein family/group databases

CAZyiGH72. Glycoside Hydrolase Family 72.

Names & Taxonomyi

Protein namesi
Recommended name:
1,3-beta-glucanosyltransferase GAS5 (EC:2.4.1.-)
Alternative name(s):
Glycolipid-anchored surface protein 5
Gene namesi
Name:GAS5
Ordered Locus Names:YOL030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL030w.
SGDiS000005390. GAS5.

Subcellular locationi

Secretedcell wall 1 Publication. Membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP).

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 4624431,3-beta-glucanosyltransferase GAS5PRO_0000010481Add
BLAST
Propeptidei463 – 48422Removed in mature formSequence AnalysisPRO_0000010482Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi299 – 2991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi344 – 3441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi359 – 3591N-linked (GlcNAc...)Sequence Analysis
Lipidationi462 – 4621GPI-anchor amidated glycineSequence Analysis

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ08193.
PaxDbiQ08193.
PeptideAtlasiQ08193.

Expressioni

Gene expression databases

GenevestigatoriQ08193.

Interactioni

Protein-protein interaction databases

BioGridi34370. 49 interactions.
DIPiDIP-4240N.
IntActiQ08193. 2 interactions.
MINTiMINT-545137.
STRINGi4932.YOL030W.

Structurei

3D structure databases

ProteinModelPortaliQ08193.
SMRiQ08193. Positions 23-366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni116 – 1249Donor substrate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi395 – 4039Asp/Glu-rich (highly acidic)
Compositional biasi405 – 46056Ser-richAdd
BLAST
Compositional biasi405 – 42723Poly-SerAdd
BLAST
Compositional biasi432 – 4398Poly-Ser

Sequence similaritiesi

Belongs to the glycosyl hydrolase 72 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG73259.
GeneTreeiENSGT00390000011003.
HOGENOMiHOG000164982.
InParanoidiQ08193.
OMAiTSGWDQK.
OrthoDBiEOG7K3TW7.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR004886. Glucanosyltransferase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF03198. Glyco_hydro_72. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08193-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLRSLTSAF VLSAGLAQAA SSSNSSTPSI EIKGNAFFNS ESGERFYIRG
60 70 80 90 100
VDYQPGGSSN LTDPLADASV CDRDVPVLKD LGINTVRVYT VDNSQDHSHC
110 120 130 140 150
MKLLQENGIY LILDVNTPTS AISRYDPACS YNADYLQNVF ATIDTFADYD
160 170 180 190 200
NVLGFFAGNE VINSVNTTNT ATYVKAVVRD MKKYIKARKY RQIPVGYSAA
210 220 230 240 250
DIVANRQLAA EYFNCGDEAD ARIDMFGVND YSWCGESSFV VSGYSTKMKL
260 270 280 290 300
YQDYSVPVFL SEFGCNQVKS SRPFTEIEAI YSTQMSSVFS GGLVYEYSNE
310 320 330 340 350
TNNYGLVQID GDKVTKLTDF ENLKNEYSKV SNPEGNGGYS TSNNYSTCPD
360 370 380 390 400
YEKGVWEANN TLPAMPSAAS AYFTSGAGSP MGTGIATQQS CDAKDDDDEE
410 420 430 440 450
DDDTSSSSSS SSSSSSSASS SSESSSSTSK ASSSSPSASE TSLLKSAASA
460 470 480
TSSSQSSSKS KGAAGIIEIP LIFRALAELY NLVL
Length:484
Mass (Da):51,870
Last modified:November 1, 1997 - v1
Checksum:iE65B9F4689B8B34C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74772 Genomic DNA. Translation: CAA99030.1.
AY693093 Genomic DNA. Translation: AAT93112.1.
BK006948 Genomic DNA. Translation: DAA10751.1.
PIRiS66713.
RefSeqiNP_014612.1. NM_001183284.1.

Genome annotation databases

EnsemblFungiiYOL030W; YOL030W; YOL030W.
GeneIDi854127.
KEGGisce:YOL030W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74772 Genomic DNA. Translation: CAA99030.1 .
AY693093 Genomic DNA. Translation: AAT93112.1 .
BK006948 Genomic DNA. Translation: DAA10751.1 .
PIRi S66713.
RefSeqi NP_014612.1. NM_001183284.1.

3D structure databases

ProteinModelPortali Q08193.
SMRi Q08193. Positions 23-366.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34370. 49 interactions.
DIPi DIP-4240N.
IntActi Q08193. 2 interactions.
MINTi MINT-545137.
STRINGi 4932.YOL030W.

Protein family/group databases

CAZyi GH72. Glycoside Hydrolase Family 72.

Proteomic databases

MaxQBi Q08193.
PaxDbi Q08193.
PeptideAtlasi Q08193.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL030W ; YOL030W ; YOL030W .
GeneIDi 854127.
KEGGi sce:YOL030W.

Organism-specific databases

CYGDi YOL030w.
SGDi S000005390. GAS5.

Phylogenomic databases

eggNOGi NOG73259.
GeneTreei ENSGT00390000011003.
HOGENOMi HOG000164982.
InParanoidi Q08193.
OMAi TSGWDQK.
OrthoDBi EOG7K3TW7.

Enzyme and pathway databases

BioCyci YEAST:G3O-33446-MONOMER.

Miscellaneous databases

NextBioi 975840.

Gene expression databases

Genevestigatori Q08193.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR004886. Glucanosyltransferase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF03198. Glyco_hydro_72. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages."
    Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G.
    J. Biol. Chem. 280:20894-20901(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, GPI-ANCHOR.
  6. "The Gas family of proteins of Saccharomyces cerevisiae: characterization and evolutionary analysis."
    Ragni E., Fontaine T., Gissi C., Latge J.-P., Popolo L.
    Yeast 24:297-308(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGAS5_YEAST
AccessioniPrimary (citable) accession number: Q08193
Secondary accession number(s): D6W235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3