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Protein

1,3-beta-glucanosyltransferase GAS5

Gene

GAS5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in cell wall biosynthesis and morphogenesis.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89Donor substrate; via carbonyl oxygenBy similarity1
Binding sitei159Donor substrateBy similarity1
Active sitei160Proton donorBy similarity1
Binding sitei160Acceptor substrateBy similarity1
Binding sitei201Acceptor substrate; via carbonyl oxygenBy similarity1
Binding sitei206Acceptor substrateBy similarity1
Active sitei262NucleophileBy similarity1
Binding sitei295Donor substrateBy similarity1

GO - Molecular functioni

  • 1,3-beta-glucanosyltransferase activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-33446-MONOMER.

Protein family/group databases

CAZyiGH72. Glycoside Hydrolase Family 72.

Names & Taxonomyi

Protein namesi
Recommended name:
1,3-beta-glucanosyltransferase GAS5 (EC:2.4.1.-)
Alternative name(s):
Glycolipid-anchored surface protein 5
Gene namesi
Name:GAS5
Ordered Locus Names:YOL030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL030W.
SGDiS000005390. GAS5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001048120 – 4621,3-beta-glucanosyltransferase GAS5Add BLAST443
PropeptideiPRO_0000010482463 – 484Removed in mature formSequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...)Sequence analysis1
Glycosylationi60N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi71 ↔ 100By similarity
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi215 ↔ 348By similarity
Disulfide bondi234 ↔ 265By similarity
Glycosylationi299N-linked (GlcNAc...)Sequence analysis1
Glycosylationi344N-linked (GlcNAc...)Sequence analysis1
Glycosylationi359N-linked (GlcNAc...)Sequence analysis1
Lipidationi462GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ08193.
PRIDEiQ08193.

Interactioni

Protein-protein interaction databases

BioGridi34370. 47 interactors.
DIPiDIP-4240N.
IntActiQ08193. 2 interactors.
MINTiMINT-545137.

Structurei

3D structure databases

ProteinModelPortaliQ08193.
SMRiQ08193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi395 – 403Asp/Glu-rich (highly acidic)9
Compositional biasi405 – 460Ser-richAdd BLAST56
Compositional biasi405 – 427Poly-SerAdd BLAST23
Compositional biasi432 – 439Poly-Ser8

Sequence similaritiesi

Belongs to the glycosyl hydrolase 72 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000011003.
HOGENOMiHOG000164982.
InParanoidiQ08193.
OMAiTSGWDQK.
OrthoDBiEOG092C1TYR.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR004886. Glucanosyltransferase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF03198. Glyco_hydro_72. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRSLTSAF VLSAGLAQAA SSSNSSTPSI EIKGNAFFNS ESGERFYIRG
60 70 80 90 100
VDYQPGGSSN LTDPLADASV CDRDVPVLKD LGINTVRVYT VDNSQDHSHC
110 120 130 140 150
MKLLQENGIY LILDVNTPTS AISRYDPACS YNADYLQNVF ATIDTFADYD
160 170 180 190 200
NVLGFFAGNE VINSVNTTNT ATYVKAVVRD MKKYIKARKY RQIPVGYSAA
210 220 230 240 250
DIVANRQLAA EYFNCGDEAD ARIDMFGVND YSWCGESSFV VSGYSTKMKL
260 270 280 290 300
YQDYSVPVFL SEFGCNQVKS SRPFTEIEAI YSTQMSSVFS GGLVYEYSNE
310 320 330 340 350
TNNYGLVQID GDKVTKLTDF ENLKNEYSKV SNPEGNGGYS TSNNYSTCPD
360 370 380 390 400
YEKGVWEANN TLPAMPSAAS AYFTSGAGSP MGTGIATQQS CDAKDDDDEE
410 420 430 440 450
DDDTSSSSSS SSSSSSSASS SSESSSSTSK ASSSSPSASE TSLLKSAASA
460 470 480
TSSSQSSSKS KGAAGIIEIP LIFRALAELY NLVL
Length:484
Mass (Da):51,870
Last modified:November 1, 1997 - v1
Checksum:iE65B9F4689B8B34C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74772 Genomic DNA. Translation: CAA99030.1.
AY693093 Genomic DNA. Translation: AAT93112.1.
BK006948 Genomic DNA. Translation: DAA10751.1.
PIRiS66713.
RefSeqiNP_014612.1. NM_001183284.1.

Genome annotation databases

EnsemblFungiiYOL030W; YOL030W; YOL030W.
GeneIDi854127.
KEGGisce:YOL030W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74772 Genomic DNA. Translation: CAA99030.1.
AY693093 Genomic DNA. Translation: AAT93112.1.
BK006948 Genomic DNA. Translation: DAA10751.1.
PIRiS66713.
RefSeqiNP_014612.1. NM_001183284.1.

3D structure databases

ProteinModelPortaliQ08193.
SMRiQ08193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34370. 47 interactors.
DIPiDIP-4240N.
IntActiQ08193. 2 interactors.
MINTiMINT-545137.

Protein family/group databases

CAZyiGH72. Glycoside Hydrolase Family 72.

Proteomic databases

MaxQBiQ08193.
PRIDEiQ08193.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL030W; YOL030W; YOL030W.
GeneIDi854127.
KEGGisce:YOL030W.

Organism-specific databases

EuPathDBiFungiDB:YOL030W.
SGDiS000005390. GAS5.

Phylogenomic databases

GeneTreeiENSGT00390000011003.
HOGENOMiHOG000164982.
InParanoidiQ08193.
OMAiTSGWDQK.
OrthoDBiEOG092C1TYR.

Enzyme and pathway databases

BioCyciYEAST:G3O-33446-MONOMER.

Miscellaneous databases

PROiQ08193.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR004886. Glucanosyltransferase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF03198. Glyco_hydro_72. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGAS5_YEAST
AccessioniPrimary (citable) accession number: Q08193
Secondary accession number(s): D6W235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.