Reviewed, UniProtKB/Swiss-Prot Q08189 (TGM3_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein-glutamine gamma-glutamyltransferase E
      Short name=TGase E
      Short name=TGE
      Short name=TG(E)
    EC=2.3.2.13
Alternative name(s):
    Transglutaminase-3
Cleaved into the following 2 chains:
    1- Recommended name:
            Protein-glutamine gamma-glutamyltransferase E 50 kDa non-catalytic chain
    2- Recommended name:
            Protein-glutamine gamma-glutamyltransferase E 27 kDa catalytic chain

Gene names

Name:	Tgm3
Synonyms:	Tgase3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. It is responsible for the later stages of cell envelope formation in the epidermis and the hair follicle.
Catalytic activity	Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	This enzyme consists of two polypeptide chains, which are synthesized as a precursor form of a single polypeptide.
Sequence similarities	Belongs to the transglutaminase superfamily. Transglutaminase family.

Ontologies

Keywords
Biological process	Keratinization
Ligand	Calcium Metal-binding
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein Zymogen
Gene Ontology (GO)
Biological process	cell envelope organization Inferred from sequence or structural similarity. Source: UniProtKB hair cell differentiation Traceable author statement. Source: UniProtKB keratinization Inferred from electronic annotation. Source: UniProtKB-KW peptide cross-linking Traceable author statement. Source: UniProtKB protein tetramerization Inferred from sequence or structural similarity. Source: UniProtKB skin development Traceable author statement. Source: UniProtKB
Cellular component	cornified envelope Traceable author statement. Source: UniProtKB cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB extrinsic to internal side of plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	GDP binding Inferred from sequence or structural similarity. Source: UniProtKB GTP binding Inferred from sequence or structural similarity. Source: UniProtKB GTPase activity Inferred from sequence or structural similarity. Source: UniProtKB acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from sequence or structural similarity. Source: UniProtKB protein-glutamine gamma-glutamyltransferase activity Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Protein-glutamine gamma-glutamyltransferase E 50 kDa non-catalytic chain		PRO_0000033654
	Chain	471 – 693	223	Protein-glutamine gamma-glutamyltransferase E 27 kDa catalytic chain		PRO_0000033655
Sites
	Active site	273	1	By similarity
	Active site	331	1	By similarity
	Active site	354	1	By similarity
	Metal binding	394	1	Calcium By similarity
	Metal binding	396	1	Calcium By similarity
	Metal binding	444	1	Calcium By similarity
	Metal binding	449	1	Calcium By similarity
Amino acid modifications
	Modified residue	111	1	Phosphotyrosine By similarity
	Modified residue	112	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08189-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 8B3F0601735D4E84	FASTA	693	77,324
	10 20 30 40 50 60 MSALQIQNVN WQVPMNRRAH HTDKFSSQDS IVRRGQPWEI ILVCNRSLES GEDLNFIVST 70 80 90 100 110 120 GPQPSESART KAVFSISGRS TGGWNAALKA NSGNNLAIAI ASPVSAPIGL YTLSVEISSR 130 140 150 160 170 180 GRASSLKLGT FIMLFNPWLQ ADDVFMSNHA ERQEYVEEDS GIIYVGSTNR IGMVGWNFGQ 190 200 210 220 230 240 FEEDILNISL SILDRSLNFR RDPVTDVARR NDPKYVCRVL SAMINGNDDN GVISGNWSGN 250 260 270 280 290 300 YTGGVDPRTW NGSVEILKNW KKSGFRPVQF GQCWVFAGTL NTVLRCLGVR SRVITNFNSA 310 320 330 340 350 360 HDTDRNLSVD VYYDAMGNPL EKGSDSVWNF HVWNEGWFVR TDLGPTYNGW QVLDATPQER 370 380 390 400 410 420 SQGVFQCGPA SVNAIKAGDV DRNFDMIFIF AEVNADRITW IYNNRNNTQK QNSVDTHSIG 430 440 450 460 470 480 KYISTKAVGS NSRMDVTDKY KYPEGSSEER QVHQKALDKL KPNASFGATS SRNPEGEDKE 490 500 510 520 530 540 PSISGKFKVT GILAVGKEVS LSLMLKNMTN DRKTVTMNMT AWTIVYNGTL VHEVWKDSAT 550 560 570 580 590 600 ISLDPEEEIQ YPVKIAYSQY ERYLKADNMI RISAVCKVPD EAEVVVEWDV ILDNPALTLE 610 620 630 640 650 660 VLEQAHVRKP VNVQMIFSNP LDQPVNNCVL LVEGSGCSVA ASRLMCHPCV PKEKSRIRFE 670 680 690 IFPTRSGTKQ LLADFSCNKF PTIKAMLPID VSE

References

[1]	"The deduced sequence of the novel protransglutaminase E (TGase3) of human and mouse." Kim I.-G., Gorman J.J., Park S.-C., Chung S.-I., Steinert P.M. J. Biol. Chem. 268:12682-12690(1993) [PubMed: 8099584] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Epidermis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10385 mRNA. Translation: AAA40421.1.
IPI	IPI00117475.
PIR	B45991.
UniGene	Mm.2961
3D structure databases
SMR	Q08189. Positions 2-693.
ModBase	Search...
Protein-protein interaction databases
STRING	Q08189.
Proteomic databases
PRIDE	Q08189.
Genome annotation databases
Ensembl	ENSMUST00000028886; ENSMUSP00000028886; ENSMUSG00000027401; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:98732. Tgm3.
Phylogenomic databases
HOVERGEN	Q08189.
Enzyme and pathway databases
BRENDA	2.3.2.13. 244.
Gene expression databases
ArrayExpress	Q08189.
Bgee	Q08189.
CleanEx	MM_TGM3.
Genevestigator	Q08189.
GermOnline	ENSMUSG00000027401. Mus musculus.
Family and domain databases
InterPro	IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR002931. Transglutaminase-like. IPR008958. Transglutaminase_C. IPR013808. Transglutaminase_CS. IPR001102. Transglutaminase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 3 hits.
Pfam	PF00927. Transglut_C. 2 hits. PF01841. Transglut_core. 1 hit. PF00868. Transglut_N. 1 hit. [Graphical view]
SMART	SM00460. TGc. 1 hit. [Graphical view]
PROSITE	PS00547. TRANSGLUTAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

TGM3_MOUSE

Accession

Primary (citable) accession number: Q08189

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 15, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)