Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q08189

- TGM3_MOUSE

UniProt

Q08189 - TGM3_MOUSE

Protein

Protein-glutamine gamma-glutamyltransferase E

Gene

Tgm3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (31 May 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold By similarity. In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.By similarity

    Catalytic activityi

    Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.PROSITE-ProRule annotation

    Cofactori

    Binds 3 calcium ions per subunit. Binds 1 calcium ion as a zymogen, and binds 2 more calcium ions, or other divalent metal cations, after proteolytic processing By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi222 – 2221Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi225 – 2251Calcium 1By similarity
    Metal bindingi227 – 2271Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi228 – 2281Calcium 1By similarity
    Metal bindingi230 – 2301Calcium 1; via carbonyl oxygenBy similarity
    Active sitei273 – 2731PROSITE-ProRule annotation
    Metal bindingi302 – 3021Calcium 2By similarity
    Metal bindingi304 – 3041Calcium 2By similarity
    Metal bindingi306 – 3061Calcium 2By similarity
    Metal bindingi308 – 3081Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi325 – 3251Calcium 2By similarity
    Active sitei331 – 3311PROSITE-ProRule annotation
    Active sitei354 – 3541PROSITE-ProRule annotation
    Metal bindingi394 – 3941Calcium 3By similarity
    Metal bindingi416 – 4161Calcium 3; via carbonyl oxygen
    Metal bindingi444 – 4441Calcium 3By similarity
    Metal bindingi449 – 4491Calcium 3By similarity
    Sitei467 – 4682Cleavage; by CTSLBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: UniProtKB
    2. catalytic activity Source: UniProtKB
    3. GDP binding Source: Ensembl
    4. GTPase activity Source: Ensembl
    5. GTP binding Source: Ensembl
    6. magnesium ion binding Source: Ensembl
    7. protein-glutamine gamma-glutamyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. cell envelope organization Source: UniProtKB
    2. epidermis development Source: UniProtKB
    3. hair cell differentiation Source: UniProtKB
    4. keratinization Source: UniProtKB-KW
    5. keratinocyte differentiation Source: UniProtKB
    6. peptide cross-linking Source: UniProtKB
    7. protein tetramerization Source: UniProtKB
    8. skin development Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Keratinization

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-glutamine gamma-glutamyltransferase E (EC:2.3.2.13)
    Alternative name(s):
    Transglutaminase E
    Short name:
    TG(E)
    Short name:
    TGE
    Short name:
    TGase E
    Transglutaminase-3
    Short name:
    TGase-3
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Tgm3
    Synonyms:Tgase3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:98732. Tgm3.

    Subcellular locationi

    GO - Cellular componenti

    1. cornified envelope Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 467466Protein-glutamine gamma-glutamyltransferase E 50 kDa catalytic chainPRO_0000033654Add
    BLAST
    Chaini468 – 693226Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chainPRO_0000033655Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei111 – 1111PhosphotyrosineBy similarity
    Modified residuei112 – 1121PhosphothreonineBy similarity

    Post-translational modificationi

    Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Zymogen

    Proteomic databases

    MaxQBiQ08189.
    PaxDbiQ08189.
    PRIDEiQ08189.

    PTM databases

    PhosphoSiteiQ08189.

    Expressioni

    Tissue specificityi

    Expressed in skin and stomach and, at lower levels, in testis, kidney and spleen (at protein level). On the basis of its catalytic activity, detected in the epidermis, around the granular and spinous layers but not in the outermost cornified layers. In hair follicles, mainly located in the medulla and the hair cortex.2 Publications

    Developmental stagei

    Expression starts at 11.5 dpc in the early two-layered epidermis. From 12.5 dpc, mainly expressed in the periderm cells and weakly in the epidermal basal cells. After epidermis keratinization, at 15.5 to 17.5 dpc, detected in the granular, cornified layers and in the hair follicle. Also expressed in heart, lung, bone, muscle, testis and blood vessels at 12.5, 13.5, 14.5 and 16.5 dpc, respectively.1 Publication

    Gene expression databases

    BgeeiQ08189.
    CleanExiMM_TGM3.
    GenevestigatoriQ08189.

    Interactioni

    Subunit structurei

    Consists of two polypeptide chains, which are synthesized as a precursor form of a single polypeptide.

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000105928.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08189.
    SMRiQ08189. Positions 2-693.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG80379.
    GeneTreeiENSGT00740000115034.
    HOGENOMiHOG000231695.
    HOVERGENiHBG004342.
    InParanoidiA1L343.
    KOiK05620.
    OMAiEILPTRS.
    OrthoDBiEOG7WT40M.
    PhylomeDBiQ08189.
    TreeFamiTF324278.

    Family and domain databases

    Gene3Di2.60.40.10. 3 hits.
    3.90.260.10. 1 hit.
    InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002931. Transglutaminase-like.
    IPR008958. Transglutaminase_C.
    IPR013808. Transglutaminase_CS.
    IPR001102. Transglutaminase_N.
    [Graphical view]
    PANTHERiPTHR11590. PTHR11590. 1 hit.
    PfamiPF00927. Transglut_C. 2 hits.
    PF01841. Transglut_core. 1 hit.
    PF00868. Transglut_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
    SMARTiSM00460. TGc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49309. SSF49309. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q08189-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSALQIQNVN WQVPMNRRAH HTDKFSSQDS IVRRGQPWEI ILVCNRSLES    50
    GEDLNFIVST GPQPSESART KAVFSISGRS TGGWNAALKA NSGNNLAIAI 100
    ASPVSAPIGL YTLSVEISSR GRASSLKLGT FIMLFNPWLQ ADDVFMSNHA 150
    ERQEYVEEDS GIIYVGSTNR IGMVGWNFGQ FEEDILNISL SILDRSLNFR 200
    RDPVTDVARR NDPKYVCRVL SAMINGNDDN GVISGNWSGN YTGGVDPRTW 250
    NGSVEILKNW KKSGFRPVQF GQCWVFAGTL NTVLRCLGVP SRVITNFNSA 300
    HDTDRNLSVD VYYDAMGNPL EKGSDSVWNF HVWNEGWFVR TDLGPTYNGW 350
    QVLDATPQER SQGVFQCGPA SVNAIKAGDV DRNFDMIFIF AEVNADRITW 400
    IYNNRNNTQK QNSVDTHSIG KYISTKAVGS NSRMDVTDKY KYPEGSSEER 450
    QVHQKALDKL KPNASFGATS SRNPEGEDKE PSISGKFKVT GILAVGKEVS 500
    LSLMLKNMTN DRKTVTMNMT AWTIVYNGTL VHEVWKDSAT ISLDPEEEIQ 550
    YPVKIAYSQY ERYLKADNMI RITAVCKVPD EAEVVVERDV ILDNPALTLE 600
    VLEQAHVRKP VNVQMLFSNP LDQPVNNCVL LVEGSGLLRG SLKIDVPSLR 650
    PKEKSRIRFE IFPTRSGTKQ LLADFSCNKF PAIKAMLPID VSE 693
    Length:693
    Mass (Da):77,309
    Last modified:May 31, 2011 - v2
    Checksum:i834D86CD87AE0E9C
    GO

    Sequence cautioni

    The sequence AAA40421.1 differs from that shown. Reason: Frameshift at positions 637 and 650.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti290 – 2901P → R in AAA40421. (PubMed:8099584)Curated
    Sequence conflicti409 – 4091Q → P in AAI29892. (PubMed:15489334)Curated
    Sequence conflicti409 – 4091Q → P in AAI29891. (PubMed:15489334)Curated
    Sequence conflicti573 – 5731T → S in AAA40421. (PubMed:8099584)Curated
    Sequence conflicti588 – 5881R → W in AAA40421. (PubMed:8099584)Curated
    Sequence conflicti616 – 6161L → I in AAA40421. (PubMed:8099584)Curated
    Sequence conflicti639 – 6391R → G in AAA40421. (PubMed:8099584)Curated
    Sequence conflicti682 – 6821A → T in AAA40421. (PubMed:8099584)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10385 mRNA. Translation: AAA40421.1. Frameshift.
    AL808127 Genomic DNA. Translation: CAM19029.1.
    CH466519 Genomic DNA. Translation: EDL28248.1.
    BC129890 mRNA. Translation: AAI29891.1.
    BC129891 mRNA. Translation: AAI29892.1.
    CCDSiCCDS38240.1.
    PIRiB45991.
    RefSeqiNP_033400.2. NM_009374.3.
    UniGeneiMm.2961.

    Genome annotation databases

    EnsembliENSMUST00000110299; ENSMUSP00000105928; ENSMUSG00000027401.
    GeneIDi21818.
    KEGGimmu:21818.
    UCSCiuc008mig.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10385 mRNA. Translation: AAA40421.1 . Frameshift.
    AL808127 Genomic DNA. Translation: CAM19029.1 .
    CH466519 Genomic DNA. Translation: EDL28248.1 .
    BC129890 mRNA. Translation: AAI29891.1 .
    BC129891 mRNA. Translation: AAI29892.1 .
    CCDSi CCDS38240.1.
    PIRi B45991.
    RefSeqi NP_033400.2. NM_009374.3.
    UniGenei Mm.2961.

    3D structure databases

    ProteinModelPortali Q08189.
    SMRi Q08189. Positions 2-693.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000105928.

    PTM databases

    PhosphoSitei Q08189.

    Proteomic databases

    MaxQBi Q08189.
    PaxDbi Q08189.
    PRIDEi Q08189.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000110299 ; ENSMUSP00000105928 ; ENSMUSG00000027401 .
    GeneIDi 21818.
    KEGGi mmu:21818.
    UCSCi uc008mig.1. mouse.

    Organism-specific databases

    CTDi 7053.
    MGIi MGI:98732. Tgm3.

    Phylogenomic databases

    eggNOGi NOG80379.
    GeneTreei ENSGT00740000115034.
    HOGENOMi HOG000231695.
    HOVERGENi HBG004342.
    InParanoidi A1L343.
    KOi K05620.
    OMAi EILPTRS.
    OrthoDBi EOG7WT40M.
    PhylomeDBi Q08189.
    TreeFami TF324278.

    Miscellaneous databases

    NextBioi 301228.
    PROi Q08189.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q08189.
    CleanExi MM_TGM3.
    Genevestigatori Q08189.

    Family and domain databases

    Gene3Di 2.60.40.10. 3 hits.
    3.90.260.10. 1 hit.
    InterProi IPR023608. Gln_gamma-glutamylTfrase_euk.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR002931. Transglutaminase-like.
    IPR008958. Transglutaminase_C.
    IPR013808. Transglutaminase_CS.
    IPR001102. Transglutaminase_N.
    [Graphical view ]
    PANTHERi PTHR11590. PTHR11590. 1 hit.
    Pfami PF00927. Transglut_C. 2 hits.
    PF01841. Transglut_core. 1 hit.
    PF00868. Transglut_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000459. TGM_EBP42. 1 hit.
    SMARTi SM00460. TGc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49309. SSF49309. 2 hits.
    SSF81296. SSF81296. 1 hit.
    PROSITEi PS00547. TRANSGLUTAMINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The deduced sequence of the novel protransglutaminase E (TGase3) of human and mouse."
      Kim I.-G., Gorman J.J., Park S.-C., Chung S.-I., Steinert P.M.
      J. Biol. Chem. 268:12682-12690(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Epidermis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Analysis of epidermal-type transglutaminase (TGase 3) expression in mouse tissues and cell lines."
      Hitomi K., Horio Y., Ikura K., Yamanishi K., Maki M.
      Int. J. Biochem. Cell Biol. 33:491-498(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Transglutaminase 3 expression in C57BL/6J mouse embryo epidermis and the correlation with its differentiation."
      Zhang J., Zhi H.Y., Ding F., Luo A.P., Liu Z.H.
      Cell Res. 15:105-110(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    7. "Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles."
      Yamane A., Fukui M., Sugimura Y., Itoh M., Alea M.P., Thomas V., El Alaoui S., Akiyama M., Hitomi K.
      FEBS J. 277:3564-3574(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiTGM3_MOUSE
    AccessioniPrimary (citable) accession number: Q08189
    Secondary accession number(s): A1L343, A2ANC3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: May 31, 2011
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3