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Q08170

- SRSF4_HUMAN

UniProt

Q08170 - SRSF4_HUMAN

Protein

Serine/arginine-rich splicing factor 4

Gene

SRSF4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 2 (15 May 2002)
      Previous versions | rss
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    Functioni

    Plays a role in alternative splice site selection during pre-mRNA splicing. Represses the splicing of MAPT/Tau exon 10.1 Publication

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. poly(A) RNA binding Source: UniProtKB

    GO - Biological processi

    1. gene expression Source: Reactome
    2. mRNA 3'-end processing Source: Reactome
    3. mRNA export from nucleus Source: Reactome
    4. mRNA processing Source: ProtInc
    5. mRNA splicing, via spliceosome Source: Reactome
    6. negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
    7. RNA splicing Source: Reactome
    8. RNA splicing, via transesterification reactions Source: ProtInc
    9. termination of RNA polymerase II transcription Source: Reactome
    10. transcription from RNA polymerase II promoter Source: Reactome

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_1597. Transport of Mature mRNA derived from an Intron-Containing Transcript.
    REACT_1849. mRNA 3'-end processing.
    REACT_467. mRNA Splicing - Major Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/arginine-rich splicing factor 4
    Alternative name(s):
    Pre-mRNA-splicing factor SRP75
    SRP001LB
    Splicing factor, arginine/serine-rich 4
    Gene namesi
    Name:SRSF4
    Synonyms:SFRS4, SRP75
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:10786. SRSF4.

    Subcellular locationi

    Nucleus speckle 1 Publication

    GO - Cellular componenti

    1. nuclear speck Source: UniProtKB-SubCell
    2. nucleoplasm Source: Reactome
    3. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35702.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494Serine/arginine-rich splicing factor 4PRO_0000081925Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei78 – 781Phosphoserine1 Publication
    Modified residuei431 – 4311Phosphoserine2 Publications

    Post-translational modificationi

    Extensively phosphorylated on serine residues in the RS domain.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ08170.
    PaxDbiQ08170.
    PeptideAtlasiQ08170.
    PRIDEiQ08170.

    PTM databases

    PhosphoSiteiQ08170.

    Expressioni

    Gene expression databases

    ArrayExpressiQ08170.
    BgeeiQ08170.
    CleanExiHS_SFRS4.
    GenevestigatoriQ08170.

    Organism-specific databases

    HPAiHPA050975.

    Interactioni

    Subunit structurei

    Found in a pre-mRNA splicing complex with SRSF4/SFRS4, SRSF5/SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Interacts with PNN.2 Publications

    Protein-protein interaction databases

    BioGridi112327. 39 interactions.
    IntActiQ08170. 23 interactions.
    MINTiMINT-1410521.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08170.
    SMRiQ08170. Positions 3-78, 93-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 7271RRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini104 – 17774RRM 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi72 – 9726Gly-rich (hinge region)Add
    BLAST
    Compositional biasi179 – 494316Arg/Ser-rich (RS domain)Add
    BLAST

    Sequence similaritiesi

    Belongs to the splicing factor SR family.Curated
    Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0724.
    HOVERGENiHBG002295.
    InParanoidiQ08170.
    KOiK12893.
    OMAiREHAKSE.
    OrthoDBiEOG7H1JPR.
    PhylomeDBiQ08170.
    TreeFamiTF351335.

    Family and domain databases

    Gene3Di3.30.70.330. 2 hits.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF00076. RRM_1. 2 hits.
    [Graphical view]
    SMARTiSM00360. RRM. 2 hits.
    [Graphical view]
    PROSITEiPS50102. RRM. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08170-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPRVYIGRLS YQARERDVER FFKGYGKILE VDLKNGYGFV EFDDLRDADD    50
    AVYELNGKDL CGERVIVEHA RGPRRDGSYG SGRSGYGYRR SGRDKYGPPT 100
    RTEYRLIVEN LSSRCSWQDL KDYMRQAGEV TYADAHKGRK NEGVIEFVSY 150
    SDMKRALEKL DGTEVNGRKI RLVEDKPGSR RRRSYSRSRS HSRSRSRSRH 200
    SRKSRSRSGS SKSSHSKSRS RSRSGSRSRS KSRSRSQSRS RSKKEKSRSP 250
    SKEKSRSRSH SAGKSRSKSK DQAEEKIQNN DNVGKPKSRS PSRHKSKSKS 300
    RSRSQERRVE EEKRGSVSRG RSQEKSLRQS RSRSRSKGGS RSRSRSRSKS 350
    KDKRKGRKRS REESRSRSRS RSKSERSRKR GSKRDSKAGS SKKKKKEDTD 400
    RSQSRSPSRS VSKEREHAKS ESSQREGRGE SENAGTNQET RSRSRSNSKS 450
    KPNLPSESRS RSKSASKTRS RSKSRSRSAS RSPSRSRSRS HSRS 494
    Length:494
    Mass (Da):56,678
    Last modified:May 15, 2002 - v2
    Checksum:i5BBAB917C218C20A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 351N → D AA sequence (PubMed:1577277)Curated
    Sequence conflicti318 – 3225SRGRS → EQGQE in AAA36649. (PubMed:8321209)Curated
    Sequence conflicti436 – 4383TNQ → RNE in AAA36649. (PubMed:8321209)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti253 – 2531E → D.1 Publication
    Corresponds to variant rs2230679 [ dbSNP | Ensembl ].
    VAR_052230
    Natural varianti338 – 3381G → A.1 Publication
    Corresponds to variant rs2230677 [ dbSNP | Ensembl ].
    VAR_052231
    Natural varianti356 – 3561G → S.1 Publication
    Corresponds to variant rs2230678 [ dbSNP | Ensembl ].
    VAR_052232
    Natural varianti438 – 4381Q → E.
    Corresponds to variant rs1049928 [ dbSNP | Ensembl ].
    VAR_052233

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L14076 mRNA. Translation: AAA36649.1.
    BT007415 mRNA. Translation: AAP36083.1.
    AL590729, AL357500 Genomic DNA. Translation: CAI14326.1.
    AL357500, AL590729 Genomic DNA. Translation: CAH71635.1.
    BC002781 mRNA. Translation: AAH02781.1.
    AC004236 Genomic DNA. Translation: AAC04476.1.
    CCDSiCCDS333.1.
    PIRiA48133.
    RefSeqiNP_005617.2. NM_005626.4.
    UniGeneiHs.469970.

    Genome annotation databases

    EnsembliENST00000373795; ENSP00000362900; ENSG00000116350.
    GeneIDi6429.
    KEGGihsa:6429.
    UCSCiuc001bro.3. human.

    Polymorphism databases

    DMDMi20981726.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L14076 mRNA. Translation: AAA36649.1 .
    BT007415 mRNA. Translation: AAP36083.1 .
    AL590729 , AL357500 Genomic DNA. Translation: CAI14326.1 .
    AL357500 , AL590729 Genomic DNA. Translation: CAH71635.1 .
    BC002781 mRNA. Translation: AAH02781.1 .
    AC004236 Genomic DNA. Translation: AAC04476.1 .
    CCDSi CCDS333.1.
    PIRi A48133.
    RefSeqi NP_005617.2. NM_005626.4.
    UniGenei Hs.469970.

    3D structure databases

    ProteinModelPortali Q08170.
    SMRi Q08170. Positions 3-78, 93-164.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112327. 39 interactions.
    IntActi Q08170. 23 interactions.
    MINTi MINT-1410521.

    PTM databases

    PhosphoSitei Q08170.

    Polymorphism databases

    DMDMi 20981726.

    Proteomic databases

    MaxQBi Q08170.
    PaxDbi Q08170.
    PeptideAtlasi Q08170.
    PRIDEi Q08170.

    Protocols and materials databases

    DNASUi 6429.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000373795 ; ENSP00000362900 ; ENSG00000116350 .
    GeneIDi 6429.
    KEGGi hsa:6429.
    UCSCi uc001bro.3. human.

    Organism-specific databases

    CTDi 6429.
    GeneCardsi GC01M029474.
    H-InvDB HIX0160019.
    HGNCi HGNC:10786. SRSF4.
    HPAi HPA050975.
    MIMi 601940. gene.
    neXtProti NX_Q08170.
    PharmGKBi PA35702.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0724.
    HOVERGENi HBG002295.
    InParanoidi Q08170.
    KOi K12893.
    OMAi REHAKSE.
    OrthoDBi EOG7H1JPR.
    PhylomeDBi Q08170.
    TreeFami TF351335.

    Enzyme and pathway databases

    Reactomei REACT_1597. Transport of Mature mRNA derived from an Intron-Containing Transcript.
    REACT_1849. mRNA 3'-end processing.
    REACT_467. mRNA Splicing - Major Pathway.

    Miscellaneous databases

    ChiTaRSi SRSF4. human.
    GeneWikii SFRS4.
    GenomeRNAii 6429.
    NextBioi 24969.
    PROi Q08170.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q08170.
    Bgeei Q08170.
    CleanExi HS_SFRS4.
    Genevestigatori Q08170.

    Family and domain databases

    Gene3Di 3.30.70.330. 2 hits.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF00076. RRM_1. 2 hits.
    [Graphical view ]
    SMARTi SM00360. RRM. 2 hits.
    [Graphical view ]
    PROSITEi PS50102. RRM. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human SR proteins and isolation of a cDNA encoding SRp75."
      Zahler A.M., Neugebauer K.M., Stolk J.A., Roth M.B.
      Mol. Cell. Biol. 13:4023-4028(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, VARIANTS ASP-253; ALA-338 AND SER-356.
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lymph.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-192.
    6. "SR proteins: a conserved family of pre-mRNA splicing factors."
      Zahler A.M., Lane W.S., Stolk J.A., Roth M.B.
      Genes Dev. 6:837-847(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 35-45; 84-89; 126-137; 140-154 AND 172-179.
    7. Cited for: IDENTIFICATION IN A MRNA SPLICING COMPLEX WITH SRSF5; SNRNP70; SNRPA1; SRRM1 AND SRRM2.
    8. "Tau exon 10, whose missplicing causes frontotemporal dementia, is regulated by an intricate interplay of cis elements and trans factors."
      Wang J., Gao Q.S., Wang Y., Lafyatis R., Stamm S., Andreadis A.
      J. Neurochem. 88:1078-1090(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Pinin/DRS/memA interacts with SRp75, SRm300 and SRrp130 in corneal epithelial cells."
      Zimowska G., Shi J., Munguba G., Jackson M.R., Alpatov R., Simmons M.N., Shi Y., Sugrue S.P.
      Invest. Ophthalmol. Vis. Sci. 44:4715-4723(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PNN, SUBCELLULAR LOCATION.
    10. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78 AND SER-431, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-431, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSRSF4_HUMAN
    AccessioniPrimary (citable) accession number: Q08170
    Secondary accession number(s): Q5VXP1, Q9BUA4, Q9UEB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: May 15, 2002
    Last modified: October 1, 2014
    This is version 143 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3