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Protein

Serine/arginine-rich splicing factor 4

Gene

SRSF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in alternative splice site selection during pre-mRNA splicing. Represses the splicing of MAPT/Tau exon 10.1 Publication

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • sequence-specific mRNA binding Source: Ensembl

GO - Biological processi

  • mRNA 3'-end processing Source: Reactome
  • mRNA export from nucleus Source: Reactome
  • mRNA processing Source: ProtInc
  • mRNA splicing, via spliceosome Source: Reactome
  • negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • response to insulin Source: Ensembl
  • RNA export from nucleus Source: Reactome
  • RNA splicing Source: ProtInc
  • RNA splicing, via transesterification reactions Source: ProtInc
  • termination of RNA polymerase II transcription Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000116350-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 4
Alternative name(s):
Pre-mRNA-splicing factor SRP75
SRP001LB
Splicing factor, arginine/serine-rich 4
Gene namesi
Name:SRSF4
Synonyms:SFRS4, SRP75
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:10786. SRSF4.

Subcellular locationi

GO - Cellular componenti

  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6429.
OpenTargetsiENSG00000116350.
PharmGKBiPA35702.

Polymorphism and mutation databases

BioMutaiSRSF4.
DMDMi20981726.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000819251 – 494Serine/arginine-rich splicing factor 4Add BLAST494

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78PhosphoserineCombined sources1
Modified residuei84PhosphoserineCombined sources1
Modified residuei288PhosphoserineCombined sources1
Modified residuei290PhosphoserineCombined sources1
Modified residuei292PhosphoserineCombined sources1
Modified residuei431PhosphoserineCombined sources1
Modified residuei446PhosphoserineCombined sources1
Modified residuei456PhosphoserineCombined sources1
Modified residuei458PhosphoserineCombined sources1
Modified residuei460PhosphoserineCombined sources1

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ08170.
MaxQBiQ08170.
PaxDbiQ08170.
PeptideAtlasiQ08170.
PRIDEiQ08170.

PTM databases

iPTMnetiQ08170.
PhosphoSitePlusiQ08170.
SwissPalmiQ08170.

Expressioni

Gene expression databases

BgeeiENSG00000116350.
CleanExiHS_SFRS4.
ExpressionAtlasiQ08170. baseline and differential.
GenevisibleiQ08170. HS.

Organism-specific databases

HPAiHPA050975.

Interactioni

Subunit structurei

Found in a pre-mRNA splicing complex with SRSF4/SFRS4, SRSF5/SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Interacts with PNN.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TRA2BP629953EBI-722621,EBI-725485

Protein-protein interaction databases

BioGridi112327. 41 interactors.
IntActiQ08170. 28 interactors.
MINTiMINT-1410521.
STRINGi9606.ENSP00000362900.

Structurei

3D structure databases

ProteinModelPortaliQ08170.
SMRiQ08170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 72RRM 1PROSITE-ProRule annotationAdd BLAST71
Domaini104 – 177RRM 2PROSITE-ProRule annotationAdd BLAST74

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi72 – 97Gly-rich (hinge region)Add BLAST26
Compositional biasi179 – 494Arg/Ser-rich (RS domain)Add BLAST316

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0106. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00700000104103.
HOVERGENiHBG002295.
InParanoidiQ08170.
KOiK12893.
OMAiKSKDHAE.
OrthoDBiEOG091G0R9W.
PhylomeDBiQ08170.
TreeFamiTF351335.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRVYIGRLS YQARERDVER FFKGYGKILE VDLKNGYGFV EFDDLRDADD
60 70 80 90 100
AVYELNGKDL CGERVIVEHA RGPRRDGSYG SGRSGYGYRR SGRDKYGPPT
110 120 130 140 150
RTEYRLIVEN LSSRCSWQDL KDYMRQAGEV TYADAHKGRK NEGVIEFVSY
160 170 180 190 200
SDMKRALEKL DGTEVNGRKI RLVEDKPGSR RRRSYSRSRS HSRSRSRSRH
210 220 230 240 250
SRKSRSRSGS SKSSHSKSRS RSRSGSRSRS KSRSRSQSRS RSKKEKSRSP
260 270 280 290 300
SKEKSRSRSH SAGKSRSKSK DQAEEKIQNN DNVGKPKSRS PSRHKSKSKS
310 320 330 340 350
RSRSQERRVE EEKRGSVSRG RSQEKSLRQS RSRSRSKGGS RSRSRSRSKS
360 370 380 390 400
KDKRKGRKRS REESRSRSRS RSKSERSRKR GSKRDSKAGS SKKKKKEDTD
410 420 430 440 450
RSQSRSPSRS VSKEREHAKS ESSQREGRGE SENAGTNQET RSRSRSNSKS
460 470 480 490
KPNLPSESRS RSKSASKTRS RSKSRSRSAS RSPSRSRSRS HSRS
Length:494
Mass (Da):56,678
Last modified:May 15, 2002 - v2
Checksum:i5BBAB917C218C20A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35N → D AA sequence (PubMed:1577277).Curated1
Sequence conflicti318 – 322SRGRS → EQGQE in AAA36649 (PubMed:8321209).Curated5
Sequence conflicti436 – 438TNQ → RNE in AAA36649 (PubMed:8321209).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052230253E → D.1 PublicationCorresponds to variant rs2230679dbSNPEnsembl.1
Natural variantiVAR_052231338G → A.1 PublicationCorresponds to variant rs2230677dbSNPEnsembl.1
Natural variantiVAR_052232356G → S.1 PublicationCorresponds to variant rs2230678dbSNPEnsembl.1
Natural variantiVAR_052233438Q → E.Corresponds to variant rs1049928dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14076 mRNA. Translation: AAA36649.1.
BT007415 mRNA. Translation: AAP36083.1.
AL590729, AL357500 Genomic DNA. Translation: CAI14326.1.
AL357500, AL590729 Genomic DNA. Translation: CAH71635.1.
BC002781 mRNA. Translation: AAH02781.1.
AC004236 Genomic DNA. Translation: AAC04476.1.
CCDSiCCDS333.1.
PIRiA48133.
RefSeqiNP_005617.2. NM_005626.4.
UniGeneiHs.469970.

Genome annotation databases

EnsembliENST00000373795; ENSP00000362900; ENSG00000116350.
GeneIDi6429.
KEGGihsa:6429.
UCSCiuc001bro.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14076 mRNA. Translation: AAA36649.1.
BT007415 mRNA. Translation: AAP36083.1.
AL590729, AL357500 Genomic DNA. Translation: CAI14326.1.
AL357500, AL590729 Genomic DNA. Translation: CAH71635.1.
BC002781 mRNA. Translation: AAH02781.1.
AC004236 Genomic DNA. Translation: AAC04476.1.
CCDSiCCDS333.1.
PIRiA48133.
RefSeqiNP_005617.2. NM_005626.4.
UniGeneiHs.469970.

3D structure databases

ProteinModelPortaliQ08170.
SMRiQ08170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112327. 41 interactors.
IntActiQ08170. 28 interactors.
MINTiMINT-1410521.
STRINGi9606.ENSP00000362900.

PTM databases

iPTMnetiQ08170.
PhosphoSitePlusiQ08170.
SwissPalmiQ08170.

Polymorphism and mutation databases

BioMutaiSRSF4.
DMDMi20981726.

Proteomic databases

EPDiQ08170.
MaxQBiQ08170.
PaxDbiQ08170.
PeptideAtlasiQ08170.
PRIDEiQ08170.

Protocols and materials databases

DNASUi6429.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373795; ENSP00000362900; ENSG00000116350.
GeneIDi6429.
KEGGihsa:6429.
UCSCiuc001bro.4. human.

Organism-specific databases

CTDi6429.
DisGeNETi6429.
GeneCardsiSRSF4.
H-InvDBHIX0160019.
HGNCiHGNC:10786. SRSF4.
HPAiHPA050975.
MIMi601940. gene.
neXtProtiNX_Q08170.
OpenTargetsiENSG00000116350.
PharmGKBiPA35702.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0106. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00700000104103.
HOVERGENiHBG002295.
InParanoidiQ08170.
KOiK12893.
OMAiKSKDHAE.
OrthoDBiEOG091G0R9W.
PhylomeDBiQ08170.
TreeFamiTF351335.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000116350-MONOMER.
ReactomeiR-HSA-109688. Cleavage of Growing Transcript in the Termination Region.
R-HSA-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72187. mRNA 3'-end processing.

Miscellaneous databases

ChiTaRSiSRSF4. human.
GeneWikiiSFRS4.
GenomeRNAii6429.
PROiQ08170.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116350.
CleanExiHS_SFRS4.
ExpressionAtlasiQ08170. baseline and differential.
GenevisibleiQ08170. HS.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRSF4_HUMAN
AccessioniPrimary (citable) accession number: Q08170
Secondary accession number(s): Q5VXP1, Q9BUA4, Q9UEB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 15, 2002
Last modified: November 30, 2016
This is version 166 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.