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Protein

Mast/stem cell growth factor receptor Kit

Gene

KIT

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. KIT promotes activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi554 – 5541MagnesiumBy similarity
Binding sitei609 – 6091ATPPROSITE-ProRule annotation
Active sitei777 – 7771Proton acceptorPROSITE-ProRule annotation
Binding sitei781 – 7811ATPPROSITE-ProRule annotation
Metal bindingi782 – 7821MagnesiumBy similarity
Metal bindingi795 – 7951MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi582 – 5898ATPPROSITE-ProRule annotation
Nucleotide bindingi657 – 6637ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 1306.
ReactomeiR-GGA-1257604. PIP3 activates AKT signaling.
R-GGA-1433557. Signaling by SCF-KIT.
R-GGA-1433559. Regulation of KIT signaling.
R-GGA-5673001. RAF/MAP kinase cascade.
R-GGA-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Mast/stem cell growth factor receptor Kit (EC:2.7.10.1)
Short name:
SCFR
Alternative name(s):
Proto-oncogene c-Kit
Tyrosine-protein kinase Kit
Gene namesi
Name:KIT
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 511487ExtracellularSequence analysisAdd
BLAST
Transmembranei512 – 53221HelicalSequence analysisAdd
BLAST
Topological domaini533 – 960428CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 960936Mast/stem cell growth factor receptor KitPRO_0000016756Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi45 ↔ 87PROSITE-ProRule annotation
Glycosylationi76 – 761N-linked (GlcNAc...)Sequence analysis
Disulfide bondi126 ↔ 175PROSITE-ProRule annotation
Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence analysis
Disulfide bondi141 ↔ 172PROSITE-ProRule annotation
Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence analysis
Disulfide bondi222 ↔ 276PROSITE-ProRule annotation
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence analysis
Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence analysis
Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence analysis
Glycosylationi318 – 3181N-linked (GlcNAc...)Sequence analysis
Glycosylationi338 – 3381N-linked (GlcNAc...)Sequence analysis
Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence analysis
Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence analysis
Disulfide bondi414 ↔ 481PROSITE-ProRule annotation
Glycosylationi453 – 4531N-linked (GlcNAc...)Sequence analysis
Glycosylationi469 – 4691N-linked (GlcNAc...)Sequence analysis
Modified residuei554 – 5541Phosphotyrosine; by autocatalysisBy similarity
Modified residuei556 – 5561Phosphotyrosine; by autocatalysisBy similarity
Modified residuei689 – 6891Phosphotyrosine; by autocatalysisBy similarity
Modified residuei706 – 7061Phosphotyrosine; by autocatalysisBy similarity
Modified residuei808 – 8081Phosphotyrosine; by autocatalysisBy similarity
Modified residuei921 – 9211Phosphotyrosine; by autocatalysisBy similarity

Post-translational modificationi

Ubiquitinated. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.By similarity
Autophosphorylated on tyrosine residues. KITLG/SCF binding promotes autophosphorylation. Phosphorylated tyrosine residues are important for interaction with specific binding partners (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ08156.
PRIDEiQ08156.

Expressioni

Tissue specificityi

High in the brain and testes and also present in the bursa of Fabricus, heart, kidney, lung, spleen thymus and ovary.

Gene expression databases

ExpressionAtlasiQ08156. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000022531.

Structurei

3D structure databases

ProteinModelPortaliQ08156.
SMRiQ08156. Positions 533-916.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 10276Ig-like C2-type 1Add
BLAST
Domaini111 – 19484Ig-like C2-type 2Add
BLAST
Domaini201 – 29494Ig-like C2-type 3Add
BLAST
Domaini303 – 39694Ig-like C2-type 4Add
BLAST
Domaini399 – 49799Ig-like C2-type 5Add
BLAST
Domaini575 – 913339Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0200. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000112008.
HOVERGENiHBG004335.
InParanoidiQ08156.
KOiK05091.
PhylomeDBiQ08156.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013151. Immunoglobulin.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR027263. SCGF_receptor.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016243. Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824. Tyr_kinase_rcpt_3_CS.
[Graphical view]
PfamiPF00047. ig. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PIRSFiPIRSF500951. SCGF_recepter. 1 hit.
PIRSF000615. TyrPK_CSF1-R. 1 hit.
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 3 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGAHLAWEL AHAVLLLSLI PAGGSVPHEE SSLVVNKGEE LRLKCNEEGP
60 70 80 90 100
VTWNFQNSDP SAKTRISNEK EWHTKNATIR DIGRYECKSK GSIVNSFYVF
110 120 130 140 150
VKDPNVLFLV DSLIYGKEDS DILLVCPLTD PDVLNFTLRK CDGKPLPKNM
160 170 180 190 200
TFIPNPQKGI IIKNVQRSFK GCYQCLAKHN GVEKISEHIF LNVRPVHKAL
210 220 230 240 250
PVITLSKSYE LLKEGEEFEV TCIITDVDSS VKASWISYKS AIVTSKSRNL
260 270 280 290 300
GDYGYERKLT LNIRSVGVND SGEFTCQAEN PFGKTNATVT LKALAKGFVR
310 320 330 340 350
LFATMNTTID INAGQNGNLT VEYEAYPKPK EEVWMYMNET LQNSSDHYVK
360 370 380 390 400
FKTVGNNSYT SELHLTRLKG TEGGIYTFFV SNSDASSSVT FNVYVKTKPE
410 420 430 440 450
ILTLDMLGND ILQCVATGFP APTIYWYFCP GTEQRCLDSP TISPMDVKVS
460 470 480 490 500
YTNSSVPSFE RILVESTVNA SMFKSTGTIC CEASSNGDKS SVFFNFAIKE
510 520 530 540 550
QIRTHTLFTP LLIAFGVAAG LMCIIVMILV YIYLQKPKYE VQWKVVEEIN
560 570 580 590 600
GNNYVYIDPT QLPYDHKWEF PRNRLSFGKT LGAGAFGKVV EATAYGLFKS
610 620 630 640 650
DAAMTVAVKM LKPSAHLTER EALMSELKVL SYLGNHINIV NLLGACTIGG
660 670 680 690 700
PTLVITEYCC YGDLLNFLRR KRDSFICPKH EEHAEAAVYE NLLHQAEPTA
710 720 730 740 750
DAVNEYMDMK PGVSYAVPPK ADKKRPVKSG SYTDQDVTLS MLEDDELALD
760 770 780 790 800
VEDLLSFSYQ VAKGMSFLAS KNCIHRDLAA RNILLTHGRI TKICDFGLAR
810 820 830 840 850
DIRNDSNYVV KGNARLPVKW MAPESIFNCV YTFESDVWSY GILLWELFSL
860 870 880 890 900
GSSPYPGMPV DSKFYKMIKE GYRMFSPECS PPEMYDIMKS CWDADPLQRP
910 920 930 940 950
TFKQIVQLIE QQLSDNAPRV YANFSTPPST QGNATDHSVR INSVGSSASS
960
TQPLLVREDV
Length:960
Mass (Da):107,312
Last modified:February 1, 1995 - v1
Checksum:i0E93850527AB68F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13225 mRNA. Translation: BAA02506.1.
PIRiJN0677.
RefSeqiNP_989692.1. NM_204361.1.
UniGeneiGga.606.

Genome annotation databases

GeneIDi378783.
KEGGigga:378783.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13225 mRNA. Translation: BAA02506.1.
PIRiJN0677.
RefSeqiNP_989692.1. NM_204361.1.
UniGeneiGga.606.

3D structure databases

ProteinModelPortaliQ08156.
SMRiQ08156. Positions 533-916.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000022531.

Proteomic databases

PaxDbiQ08156.
PRIDEiQ08156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi378783.
KEGGigga:378783.

Organism-specific databases

CTDi3815.

Phylogenomic databases

eggNOGiKOG0200. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000112008.
HOVERGENiHBG004335.
InParanoidiQ08156.
KOiK05091.
PhylomeDBiQ08156.

Enzyme and pathway databases

BRENDAi2.7.10.1. 1306.
ReactomeiR-GGA-1257604. PIP3 activates AKT signaling.
R-GGA-1433557. Signaling by SCF-KIT.
R-GGA-1433559. Regulation of KIT signaling.
R-GGA-5673001. RAF/MAP kinase cascade.
R-GGA-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Miscellaneous databases

PROiQ08156.

Gene expression databases

ExpressionAtlasiQ08156. baseline and differential.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013151. Immunoglobulin.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR027263. SCGF_receptor.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016243. Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824. Tyr_kinase_rcpt_3_CS.
[Graphical view]
PfamiPF00047. ig. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PIRSFiPIRSF500951. SCGF_recepter. 1 hit.
PIRSF000615. TyrPK_CSF1-R. 1 hit.
SMARTiSM00409. IG. 4 hits.
SM00408. IGc2. 3 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 5 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of the chicken c-kit proto-oncogene."
    Sasaki E., Okamura H., Chikamune T., Kanai Y., Watanabe M., Naito M., Sakurai M.
    Gene 128:257-261(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: White leghorn.
    Tissue: Brain.

Entry informationi

Entry nameiKIT_CHICK
AccessioniPrimary (citable) accession number: Q08156
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.