Q08144 (TLG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-SNARE affecting a late Golgi compartment protein 2

Alternative name(s):
Syntaxin TLG2

Gene names

Name:	TLG2
Ordered Locus Names:	YOL018C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	t-SNARE that functions in transport from the endosome to the late Golgi and on the endocytic pathway.
Subunit structure	Interacts with VPS45. Ref.6
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Single-pass type IV membrane protein. Endosome membrane; Single-pass type IV membrane protein.
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Golgi apparatus Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	CVT pathway Inferred from mutant phenotype. Source: SGD Golgi to vacuole transport Inferred from genetic interaction. Source: SGD endocytosis Inferred from mutant phenotype. Source: SGD inositol phosphorylceramide metabolic process Inferred from mutant phenotype. Source: SGD mannosyl-inositol phosphorylceramide metabolic process Inferred from mutant phenotype. Source: SGD protein exit from endoplasmic reticulum Inferred from genetic interaction. Source: SGD vesicle fusion Inferred from direct assay. Source: SGD
Cellular component	integral to endosome membrane Inferred from direct assay Ref.4. Source: SGD trans-Golgi network Inferred from direct assay. Source: SGD
Molecular function	SNAP receptor activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VPS45	P38932	3	EBI-19302,EBI-20444

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	T-SNARE affecting a late Golgi compartment protein 2		PRO_0000210279
Regions
	Topological domain	1 – 317	317	Cytoplasmic Potential
	Transmembrane	318 – 338	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	339 – 397	59	Vesicular Potential
	Domain	244 – 306	63	t-SNARE coiled-coil homology
	Coiled coil	74 – 96	23	Potential
Experimental info
	Sequence conflict	397	1	L → R in AAT93237. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q08144 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CEBD6FE11278B1F0	FASTA	397	45,875
	10 20 30 40 50 60 MFRDRTNLFL SYRRTFPHNI TFSSGKAPLG DDQDIEMGTY PMMNMSHDIS ARLTDERKNK 70 80 90 100 110 120 HENHSDALPP IFIDIAQDVD DYLLEVRRLS EQLAKVYRKN SLPGFEDKSH DEALIEDLSF 130 140 150 160 170 180 KVIQMLQKCY AVMKRLKTIY NSQFVDGKQL SREELIILDN LQKIYAEKIQ TESNKFRVLQ 190 200 210 220 230 240 NNYLKFLNKD DLKPIRNKAS AENTLLLDDE EEEAAREKRE GLDIEDYSKR TLQRQQQLHD 250 260 270 280 290 300 TSAEAYLRER DEEITQLARG VLEVSTIFRE MQDLVVDQGT IVDRIDYNLE NTVVELKSAD 310 320 330 340 350 360 KELNKATHYQ KRTQKCKVIL LLTLCVIALF FFVMLKPHGG GSGGRNNGSN KYNNDDNKTV 370 380 390 NNSHDDGSNT HINDEESNLP SIVEVTESEN DALDDLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Tlg2p, a yeast syntaxin homolog that resides on the Golgi and endocytic structures." Abeliovich H., Grote E., Novick P., Ferro-Novick S. J. Biol. Chem. 273:11719-11727(1998) [PubMed: 9565594] [Abstract] Cited for: CHARACTERIZATION.
[5]	"A yeast t-SNARE involved in endocytosis." Seron K., Tieaho V., Prescianotto-Baschong C., Aust T., Blondel M.O., Guillaud P., Devilliers G., Rossanese O.W., Glick B.S., Riezman H., Keranen S., Haguenauer-Tsapis R. Mol. Biol. Cell 9:2873-2889(1998) [PubMed: 9763449] [Abstract] Cited for: CHARACTERIZATION.
[6]	"The Sec1p homologue Vps45p binds to the syntaxin Tlg2p." Nichols B.J., Holthuis J.C., Pelham H.R. Eur. J. Cell Biol. 77:263-268(1998) [PubMed: 9930650] [Abstract] Cited for: INTERACTION WITH VPS45.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74760 Genomic DNA. Translation: CAA99017.1. AY693218 Genomic DNA. Translation: AAT93237.1. BK006948 Genomic DNA. Translation: DAA10764.1.
PIR	S66700.
RefSeq	NP_014624.1. NM_001183272.1.
3D structure databases
ProteinModelPortal	Q08144.
SMR	Q08144. Positions 249-314.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4244N.
IntAct	Q08144. 7 interactions.
MINT	MINT-476443.
STRING	Q08144.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOL018C; YOL018C; YOL018C.
GeneID	854142.
KEGG	sce:YOL018C.
NMPDR	fig|4932.3.peg.5718.
Organism-specific databases
CYGD	YOL018c.
SGD	S000005378. TLG2.
Phylogenomic databases
eggNOG	fuNOG04186.
HOGENOM	HBG736421.
OMA	CVFALLM.
OrthoDB	EOG42NN91.
Gene expression databases
ArrayExpress	Q08144.
Genevestigator	Q08144.
GermOnline	YOL018C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006012. Syntaxin/epimorphin_CS. IPR010989. t-SNARE. IPR000727. T_SNARE_dom. [Graphical view]
KO	K08489.
Pfam	PF05739. SNARE. 1 hit. [Graphical view]
SMART	SM00397. t_SNARE. 1 hit. [Graphical view]
SUPFAM	SSF47661. t-snare. 1 hit.
PROSITE	PS00914. SYNTAXIN. 1 hit. PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975882.

Entry information

Entry name

TLG2_YEAST

Accession

Primary (citable) accession number: Q08144
Secondary accession number(s): D6W248, E9P933

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program