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Protein

Transducin-like enhancer protein 3

Gene

Tle3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity). May play an important role during spermatogenesis.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Spermatogenesis, Transcription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.
R-MMU-3769402. Deactivation of the beta-catenin transactivating complex.
R-MMU-4641265. Repression of WNT target genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Transducin-like enhancer protein 3
Alternative name(s):
ESG
Grg-3
Gene namesi
Name:Tle3
Synonyms:Esg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:104634. Tle3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 772772Transducin-like enhancer protein 3PRO_0000051281Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei203 – 2031PhosphoserineCombined sources
Modified residuei207 – 2071PhosphoserineBy similarity
Modified residuei211 – 2111PhosphoserineBy similarity
Modified residuei217 – 2171PhosphoserineBy similarity
Modified residuei232 – 2321N6-acetyllysineBy similarity
Modified residuei240 – 2401PhosphoserineBy similarity
Modified residuei245 – 2451PhosphoserineBy similarity
Modified residuei259 – 2591PhosphothreonineCombined sources
Modified residuei263 – 2631PhosphoserineCombined sources
Modified residuei267 – 2671PhosphoserineCombined sources
Modified residuei275 – 2751N6-acetyllysineBy similarity
Modified residuei286 – 2861PhosphoserineCombined sources
Modified residuei312 – 3121PhosphothreonineBy similarity
Modified residuei317 – 3171PhosphoserineBy similarity
Modified residuei319 – 3191PhosphothreonineBy similarity
Modified residuei321 – 3211PhosphothreonineBy similarity
Modified residuei328 – 3281PhosphothreonineCombined sources
Modified residuei334 – 3341PhosphothreonineCombined sources
Modified residuei413 – 4131PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ08122.
MaxQBiQ08122.
PaxDbiQ08122.
PRIDEiQ08122.

PTM databases

iPTMnetiQ08122.
PhosphoSiteiQ08122.

Expressioni

Tissue specificityi

Expressed only in testis.

Gene expression databases

BgeeiQ08122.
ExpressionAtlasiQ08122. baseline and differential.
GenevisibleiQ08122. MM.

Interactioni

Subunit structurei

Homotetramer and heterooligomer with other family members. Binds FOXA2 (By similarity). Interacts with XIAP/BIRC4 (By similarity). Binds LEF1, TCF7, TCF7L1 and TCF7L2/TCF4. Interacts with TBX18 (via engrailed homology 1 repressor motif), leading to decreased of TBX18 transcriptional activity.By similarity2 Publications

Protein-protein interaction databases

BioGridi204218. 1 interaction.
STRINGi10090.ENSMUSP00000124131.

Structurei

3D structure databases

ProteinModelPortaliQ08122.
SMRiQ08122. Positions 19-135, 443-772.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati484 – 52239WD 1Add
BLAST
Repeati530 – 56940WD 2Add
BLAST
Repeati574 – 61340WD 3Add
BLAST
Repeati616 – 65540WD 4Add
BLAST
Repeati657 – 69640WD 5Add
BLAST
Repeati698 – 73740WD 6Add
BLAST
Repeati739 – 77133WD 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 131131Q domainBy similarityAdd
BLAST
Regioni132 – 19968GP domainBy similarityAdd
BLAST
Regioni200 – 26869CcN domainBy similarityAdd
BLAST
Regioni269 – 452184SP domainBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi225 – 2284Nuclear localization signalSequence analysis

Domaini

WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.1 Publication

Sequence similaritiesi

Belongs to the WD repeat Groucho/TLE family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
GeneTreeiENSGT00550000074465.
HOVERGENiHBG004689.
InParanoidiQ08122.
TreeFamiTF314167.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR005617. Groucho/TLE_N.
IPR009146. Groucho_enhance.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR01850. GROUCHOFAMLY.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q08122-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYPQGRHPAP HQPGQPGFKF TVAESCDRIK DEFQFLQAQY HSLKVEYDKL
60 70 80 90 100
ANEKTEMQRH YVMYYEMSYG LNIEMHKQTE IAKRLNTILA QIMPFLSQEH
110 120 130 140 150
QQQVAQAVER AKQVTMTELN AIIGQQQLQA QHLSHATHGP PVQLPPHPSG
160 170 180 190 200
LQPPGIPPVT GSSSGLLALG ALGSQAHLAV KDEKNHHELD HRERESSTNN
210 220 230 240 250
SVSPSESLRA SEKHRGSADY SMEAKKRKAE EKDSLSRYDS DGDKSDDLVV
260 270 280 290 300
DVSNEDPATP RVSPAHSPPE NGLDKARGLK KDAPTSPASV ASSSSTPSSK
310 320 330 340 350
TKDLGHNDKS STPGLKSNTP TPRNDAPTPG TSTTPGLRSM PGKPPGMDPI
360 370 380 390 400
GIMASALRTP ITLTSSYPAP FAMMSHHEMN GSLTSPSAYA GLHNIPSQMS
410 420 430 440 450
AAAAAAAAAY GRSPMVSFGA VGFDPHPPMR ATGLPSSLAS IPGGKPAYSF
460 470 480 490 500
HVSADGQMQP VPFPHDALAG PGIPRHARQI NTLSHGEVVC AVTISNPTRH
510 520 530 540 550
VYTGGKGCVK IWDISQPGSK SPISQLDCLN RDNYIRSCKL LPDGRTLIVG
560 570 580 590 600
GEASTLTIWD LASPTPRIKA ELTSSAPACY ALAISPDAKV CFSCCSDGNI
610 620 630 640 650
AVWDLHNQTL VRQFQGHTDG ASCIDISHDG TKLWTGGLDN TVRSWDLREG
660 670 680 690 700
RQLQQHDFTS QIFSLGYCPT GEWLAVGMES SNVEVLHHTK PDKYQLHLHE
710 720 730 740 750
SCVLSLKFAY CGKWFVSTGK DNLLNAWRTP YGASIFQSKE SSSVLSCDIS
760 770
ADDKYIVTGS GDKKATVYEV IY
Length:772
Mass (Da):83,447
Last modified:November 24, 2009 - v3
Checksum:i39735F2718A8D27A
GO
Isoform 1 (identifier: Q08122-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-8: YPQGRHP → CPCGLQ

Show »
Length:771
Mass (Da):83,212
Checksum:iF8263B8A418F757A
GO
Isoform 2 (identifier: Q08122-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     351-353: Missing.

Note: No experimental confirmation available.
Show »
Length:769
Mass (Da):83,145
Checksum:i5AF18B9723D42E6C
GO
Isoform 4 (identifier: Q08122-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     124-124: G → GVRGLPNLPLT

Note: No experimental confirmation available.
Show »
Length:782
Mass (Da):84,508
Checksum:iEEE3F366B5229276
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti545 – 5451R → G in CAA51770 (PubMed:8365415).Curated
Sequence conflicti559 – 5591W → R in BAC27347 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2 – 87YPQGRHP → CPCGLQ in isoform 1. 2 PublicationsVSP_038441
Alternative sequencei124 – 1241G → GVRGLPNLPLT in isoform 4. 1 PublicationVSP_038442
Alternative sequencei351 – 3533Missing in isoform 2. 1 PublicationVSP_007025

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73360 mRNA. Translation: CAA51770.1.
AC126672 Genomic DNA. No translation available.
BC006672 mRNA. Translation: AAH06672.1.
BC056465 mRNA. Translation: AAH56465.1.
AK031322 mRNA. Translation: BAC27347.1.
CCDSiCCDS52825.1. [Q08122-3]
CCDS52826.1. [Q08122-4]
PIRiS35681.
RefSeqiNP_001077396.1. NM_001083927.1. [Q08122-4]
NP_033415.2. NM_009389.2. [Q08122-3]
XP_006511091.1. XM_006511028.2. [Q08122-2]
UniGeneiMm.24255.

Genome annotation databases

EnsembliENSMUST00000160882; ENSMUSP00000124131; ENSMUSG00000032280. [Q08122-4]
ENSMUST00000162583; ENSMUSP00000124977; ENSMUSG00000032280. [Q08122-2]
ENSMUST00000162973; ENSMUSP00000124173; ENSMUSG00000032280. [Q08122-3]
GeneIDi21887.
KEGGimmu:21887.
UCSCiuc009pzo.1. mouse. [Q08122-4]
uc009pzp.1. mouse. [Q08122-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73360 mRNA. Translation: CAA51770.1.
AC126672 Genomic DNA. No translation available.
BC006672 mRNA. Translation: AAH06672.1.
BC056465 mRNA. Translation: AAH56465.1.
AK031322 mRNA. Translation: BAC27347.1.
CCDSiCCDS52825.1. [Q08122-3]
CCDS52826.1. [Q08122-4]
PIRiS35681.
RefSeqiNP_001077396.1. NM_001083927.1. [Q08122-4]
NP_033415.2. NM_009389.2. [Q08122-3]
XP_006511091.1. XM_006511028.2. [Q08122-2]
UniGeneiMm.24255.

3D structure databases

ProteinModelPortaliQ08122.
SMRiQ08122. Positions 19-135, 443-772.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204218. 1 interaction.
STRINGi10090.ENSMUSP00000124131.

PTM databases

iPTMnetiQ08122.
PhosphoSiteiQ08122.

Proteomic databases

EPDiQ08122.
MaxQBiQ08122.
PaxDbiQ08122.
PRIDEiQ08122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000160882; ENSMUSP00000124131; ENSMUSG00000032280. [Q08122-4]
ENSMUST00000162583; ENSMUSP00000124977; ENSMUSG00000032280. [Q08122-2]
ENSMUST00000162973; ENSMUSP00000124173; ENSMUSG00000032280. [Q08122-3]
GeneIDi21887.
KEGGimmu:21887.
UCSCiuc009pzo.1. mouse. [Q08122-4]
uc009pzp.1. mouse. [Q08122-3]

Organism-specific databases

CTDi7090.
MGIiMGI:104634. Tle3.

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
GeneTreeiENSGT00550000074465.
HOVERGENiHBG004689.
InParanoidiQ08122.
TreeFamiTF314167.

Enzyme and pathway databases

ReactomeiR-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.
R-MMU-3769402. Deactivation of the beta-catenin transactivating complex.
R-MMU-4641265. Repression of WNT target genes.

Miscellaneous databases

PROiQ08122.
SOURCEiSearch...

Gene expression databases

BgeeiQ08122.
ExpressionAtlasiQ08122. baseline and differential.
GenevisibleiQ08122. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR005617. Groucho/TLE_N.
IPR009146. Groucho_enhance.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR01850. GROUCHOFAMLY.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein."
    Miyasaka H., Choudhury B.K., Hou E.W., Li S.S.-L.
    Eur. J. Biochem. 216:343-352(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: CD-1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 3).
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 324-772 (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain and Mammary tumor.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 42-772 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Testis.
  5. "All Tcf HMG box transcription factors interact with Groucho-related co-repressors."
    Brantjes H., Roose J., van De Wetering M., Clevers H.
    Nucleic Acids Res. 29:1410-1419(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LEF1; TCF7; TCF7L1 AND TCF7L2.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  7. "Transcriptional repression by the T-box proteins Tbx18 and Tbx15 depends on Groucho corepressors."
    Farin H.F., Bussen M., Schmidt M.K., Singh M.K., Schuster-Gossler K., Kispert A.
    J. Biol. Chem. 282:25748-25759(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TBX18.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. "The Groucho/TLE/Grg family of transcriptional co-repressors."
    Jennings B.H., Ish-Horowicz D.
    Genome Biol. 9:R205.1-R205.7(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203; THR-259; SER-263; SER-267; SER-286; THR-328 AND THR-334, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiTLE3_MOUSE
AccessioniPrimary (citable) accession number: Q08122
Secondary accession number(s): Q6PHP0, Q923A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 24, 2009
Last modified: July 6, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.