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Protein

Transducin-like enhancer protein 3

Gene

Tle3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity). May play an important role during spermatogenesis.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Spermatogenesis, Transcription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.
R-MMU-3769402. Deactivation of the beta-catenin transactivating complex.
R-MMU-4641265. Repression of WNT target genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Transducin-like enhancer protein 3
Alternative name(s):
ESG
Grg-3
Gene namesi
Name:Tle3
Synonyms:Esg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:104634. Tle3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512811 – 772Transducin-like enhancer protein 3Add BLAST772

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei203PhosphoserineCombined sources1
Modified residuei207PhosphoserineBy similarity1
Modified residuei211PhosphoserineBy similarity1
Modified residuei217PhosphoserineBy similarity1
Modified residuei232N6-acetyllysineBy similarity1
Modified residuei240PhosphoserineBy similarity1
Modified residuei245PhosphoserineBy similarity1
Modified residuei259PhosphothreonineCombined sources1
Modified residuei263PhosphoserineCombined sources1
Modified residuei267PhosphoserineCombined sources1
Modified residuei275N6-acetyllysineBy similarity1
Modified residuei286PhosphoserineCombined sources1
Modified residuei312PhosphothreonineBy similarity1
Modified residuei317PhosphoserineBy similarity1
Modified residuei319PhosphothreonineBy similarity1
Modified residuei321PhosphothreonineBy similarity1
Modified residuei328PhosphothreonineCombined sources1
Modified residuei334PhosphothreonineCombined sources1
Modified residuei413PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ08122.
PRIDEiQ08122.

PTM databases

iPTMnetiQ08122.
PhosphoSitePlusiQ08122.

Expressioni

Tissue specificityi

Expressed only in testis.

Gene expression databases

BgeeiENSMUSG00000032280.
ExpressionAtlasiQ08122. baseline and differential.
GenevisibleiQ08122. MM.

Interactioni

Subunit structurei

Homotetramer and heterooligomer with other family members. Binds FOXA2 (By similarity). Interacts with XIAP/BIRC4 (By similarity). Binds LEF1, TCF7, TCF7L1 and TCF7L2/TCF4. Interacts with TBX18 (via engrailed homology 1 repressor motif), leading to decreased of TBX18 transcriptional activity.By similarity2 Publications

Protein-protein interaction databases

BioGridi204218. 1 interactor.
STRINGi10090.ENSMUSP00000124131.

Structurei

3D structure databases

ProteinModelPortaliQ08122.
SMRiQ08122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati484 – 522WD 1Add BLAST39
Repeati530 – 569WD 2Add BLAST40
Repeati574 – 613WD 3Add BLAST40
Repeati616 – 655WD 4Add BLAST40
Repeati657 – 696WD 5Add BLAST40
Repeati698 – 737WD 6Add BLAST40
Repeati739 – 771WD 7Add BLAST33

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 131Q domainBy similarityAdd BLAST131
Regioni132 – 199GP domainBy similarityAdd BLAST68
Regioni200 – 268CcN domainBy similarityAdd BLAST69
Regioni269 – 452SP domainBy similarityAdd BLAST184

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi225 – 228Nuclear localization signalSequence analysis4

Domaini

WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.1 Publication

Sequence similaritiesi

Belongs to the WD repeat Groucho/TLE family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
GeneTreeiENSGT00550000074465.
HOVERGENiHBG004689.
InParanoidiQ08122.
TreeFamiTF314167.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR005617. Groucho/TLE_N.
IPR009146. Groucho_enhance.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR01850. GROUCHOFAMLY.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q08122-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYPQGRHPAP HQPGQPGFKF TVAESCDRIK DEFQFLQAQY HSLKVEYDKL
60 70 80 90 100
ANEKTEMQRH YVMYYEMSYG LNIEMHKQTE IAKRLNTILA QIMPFLSQEH
110 120 130 140 150
QQQVAQAVER AKQVTMTELN AIIGQQQLQA QHLSHATHGP PVQLPPHPSG
160 170 180 190 200
LQPPGIPPVT GSSSGLLALG ALGSQAHLAV KDEKNHHELD HRERESSTNN
210 220 230 240 250
SVSPSESLRA SEKHRGSADY SMEAKKRKAE EKDSLSRYDS DGDKSDDLVV
260 270 280 290 300
DVSNEDPATP RVSPAHSPPE NGLDKARGLK KDAPTSPASV ASSSSTPSSK
310 320 330 340 350
TKDLGHNDKS STPGLKSNTP TPRNDAPTPG TSTTPGLRSM PGKPPGMDPI
360 370 380 390 400
GIMASALRTP ITLTSSYPAP FAMMSHHEMN GSLTSPSAYA GLHNIPSQMS
410 420 430 440 450
AAAAAAAAAY GRSPMVSFGA VGFDPHPPMR ATGLPSSLAS IPGGKPAYSF
460 470 480 490 500
HVSADGQMQP VPFPHDALAG PGIPRHARQI NTLSHGEVVC AVTISNPTRH
510 520 530 540 550
VYTGGKGCVK IWDISQPGSK SPISQLDCLN RDNYIRSCKL LPDGRTLIVG
560 570 580 590 600
GEASTLTIWD LASPTPRIKA ELTSSAPACY ALAISPDAKV CFSCCSDGNI
610 620 630 640 650
AVWDLHNQTL VRQFQGHTDG ASCIDISHDG TKLWTGGLDN TVRSWDLREG
660 670 680 690 700
RQLQQHDFTS QIFSLGYCPT GEWLAVGMES SNVEVLHHTK PDKYQLHLHE
710 720 730 740 750
SCVLSLKFAY CGKWFVSTGK DNLLNAWRTP YGASIFQSKE SSSVLSCDIS
760 770
ADDKYIVTGS GDKKATVYEV IY
Length:772
Mass (Da):83,447
Last modified:November 24, 2009 - v3
Checksum:i39735F2718A8D27A
GO
Isoform 1 (identifier: Q08122-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2-8: YPQGRHP → CPCGLQ

Show »
Length:771
Mass (Da):83,212
Checksum:iF8263B8A418F757A
GO
Isoform 2 (identifier: Q08122-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     351-353: Missing.

Note: No experimental confirmation available.
Show »
Length:769
Mass (Da):83,145
Checksum:i5AF18B9723D42E6C
GO
Isoform 4 (identifier: Q08122-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     124-124: G → GVRGLPNLPLT

Note: No experimental confirmation available.
Show »
Length:782
Mass (Da):84,508
Checksum:iEEE3F366B5229276
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti545R → G in CAA51770 (PubMed:8365415).Curated1
Sequence conflicti559W → R in BAC27347 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0384412 – 8YPQGRHP → CPCGLQ in isoform 1. 2 Publications7
Alternative sequenceiVSP_038442124G → GVRGLPNLPLT in isoform 4. 1 Publication1
Alternative sequenceiVSP_007025351 – 353Missing in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73360 mRNA. Translation: CAA51770.1.
AC126672 Genomic DNA. No translation available.
BC006672 mRNA. Translation: AAH06672.1.
BC056465 mRNA. Translation: AAH56465.1.
AK031322 mRNA. Translation: BAC27347.1.
CCDSiCCDS52825.1. [Q08122-3]
CCDS52826.1. [Q08122-4]
PIRiS35681.
RefSeqiNP_001077396.1. NM_001083927.1. [Q08122-4]
NP_033415.2. NM_009389.2. [Q08122-3]
XP_006511091.1. XM_006511028.3. [Q08122-2]
UniGeneiMm.24255.

Genome annotation databases

EnsembliENSMUST00000160882; ENSMUSP00000124131; ENSMUSG00000032280. [Q08122-4]
ENSMUST00000162583; ENSMUSP00000124977; ENSMUSG00000032280. [Q08122-2]
ENSMUST00000162973; ENSMUSP00000124173; ENSMUSG00000032280. [Q08122-3]
GeneIDi21887.
KEGGimmu:21887.
UCSCiuc009pzo.1. mouse. [Q08122-4]
uc009pzp.1. mouse. [Q08122-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73360 mRNA. Translation: CAA51770.1.
AC126672 Genomic DNA. No translation available.
BC006672 mRNA. Translation: AAH06672.1.
BC056465 mRNA. Translation: AAH56465.1.
AK031322 mRNA. Translation: BAC27347.1.
CCDSiCCDS52825.1. [Q08122-3]
CCDS52826.1. [Q08122-4]
PIRiS35681.
RefSeqiNP_001077396.1. NM_001083927.1. [Q08122-4]
NP_033415.2. NM_009389.2. [Q08122-3]
XP_006511091.1. XM_006511028.3. [Q08122-2]
UniGeneiMm.24255.

3D structure databases

ProteinModelPortaliQ08122.
SMRiQ08122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204218. 1 interactor.
STRINGi10090.ENSMUSP00000124131.

PTM databases

iPTMnetiQ08122.
PhosphoSitePlusiQ08122.

Proteomic databases

PaxDbiQ08122.
PRIDEiQ08122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000160882; ENSMUSP00000124131; ENSMUSG00000032280. [Q08122-4]
ENSMUST00000162583; ENSMUSP00000124977; ENSMUSG00000032280. [Q08122-2]
ENSMUST00000162973; ENSMUSP00000124173; ENSMUSG00000032280. [Q08122-3]
GeneIDi21887.
KEGGimmu:21887.
UCSCiuc009pzo.1. mouse. [Q08122-4]
uc009pzp.1. mouse. [Q08122-3]

Organism-specific databases

CTDi7090.
MGIiMGI:104634. Tle3.

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
GeneTreeiENSGT00550000074465.
HOVERGENiHBG004689.
InParanoidiQ08122.
TreeFamiTF314167.

Enzyme and pathway databases

ReactomeiR-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.
R-MMU-3769402. Deactivation of the beta-catenin transactivating complex.
R-MMU-4641265. Repression of WNT target genes.

Miscellaneous databases

PROiQ08122.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032280.
ExpressionAtlasiQ08122. baseline and differential.
GenevisibleiQ08122. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR005617. Groucho/TLE_N.
IPR009146. Groucho_enhance.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR01850. GROUCHOFAMLY.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLE3_MOUSE
AccessioniPrimary (citable) accession number: Q08122
Secondary accession number(s): Q6PHP0, Q923A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 24, 2009
Last modified: November 2, 2016
This is version 150 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.