Q08116 (RGS1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulator of G-protein signaling 1
Short name=RGS1

Alternative name(s):
B-cell activation protein BL34
Early response protein 1R20

Gene names

Name:	RGS1
Synonyms:	1R20, BL34, IER1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. This protein may be involved in the regulation of B-cell activation and proliferation.
Tissue specificity	B-cell specific. Expression is relatively low in B-cells and chronic lymphocytic leukemia B-cells; however, in other types of malignant B-cell such as non-Hodgkin lymphoma and hairy cell leukemia, expression is constitutively high.
Induction	In response to several B-cell activation signals.
Post-translational modification	Could be phosphorylated. Might be functionally regulated by protein kinase(s).
Sequence similarities	Contains 1 RGS domain.
Sequence caution	The sequence AAB26289.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH15510.1 differs from that shown. Reason: Erroneous initiation. The sequence BAG37976.1 differs from that shown. Reason: Erroneous initiation. The sequence BAG64242.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA51826.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Signal transduction inhibitor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Traceable author statement PubMed 10480894. Source: ProtInc immune response Traceable author statement Ref.5. Source: ProtInc termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	GTPase activator activity Inferred from Biological aspect of Ancestor. Source: RefGenome calmodulin binding Traceable author statement PubMed 10747990. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q08116-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q08116-2)

The sequence of this isoform differs from the canonical sequence as follows:
     149-209: INIDFRTRES...LNDLQANSLK → VSIKLIIISFSIKDPICRNNI

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 209

209

Regulator of G-protein signaling 1

PRO_0000204175

Regions

Domain

85 – 200

116

RGS

Amino acid modifications

Modified residue

32

1

Phosphoserine; by CK2 Potential

Modified residue

67

1

Phosphoserine; by PKC Potential

Modified residue

75

1

Phosphoserine; by CK2 Potential

Modified residue

159

1

Phosphothreonine; by PKC Potential

Modified residue

169

1

Phosphothreonine; by CK2 Potential

Modified residue

180

1

Phosphothreonine; by CK2 Potential

Modified residue

207

1

Phosphoserine; by PKC Potential

Natural variations

Alternative sequence

149 – 209

61

INIDF…ANSLK → VSIKLIIISFSIKDPICRNN I in isoform 2.

VSP_036422

Experimental info

Sequence conflict

177

1

V → A in BAG37976. Ref.1

Sequence conflict

194

1

D → H in CAA51826. Ref.5

Secondary structure

1

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209

Helix Strand Turn

Details...

Beta strand

72 – 75

4

Helix

76 – 81

6

Turn

82 – 84

3

Helix

86 – 91

6

Helix

93 – 105

13

Helix

110 – 122

13

Helix

126 – 128

3

Helix

129 – 140

12

Helix

153 – 163

11

Turn

168 – 171

4

Helix

172 – 184

13

Helix

186 – 191

6

Helix

194 – 202

9

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 24, 2009. Version 3. Checksum: D8D7D8E80819496A	FASTA	209	23,858
	10 20 30 40 50 60 MRAAAISTPK LDKMPGMFFS ANPKELKGTT HSLLDDKMQK RRPKTFGMDM KAYLRSMIPH 70 80 90 100 110 120 LESGMKSSKS KDVLSAAEVM QWSQSLEKLL ANQTGQNVFG SFLKSEFSEE NIEFWLACED 130 140 150 160 170 180 YKKTESDLLP CKAEEIYKAF VHSDAAKQIN IDFRTRESTA KKIKAPTPTC FDEAQKVIYT 190 200 LMEKDSYPRF LKSDIYLNLL NDLQANSLK
	Isoform 2 [UniParc]. Checksum: 96E56F3B53747867	FASTA	169	19,134
	10 20 30 40 50 60 MRAAAISTPK LDKMPGMFFS ANPKELKGTT HSLLDDKMQK RRPKTFGMDM KAYLRSMIPH 70 80 90 100 110 120 LESGMKSSKS KDVLSAAEVM QWSQSLEKLL ANQTGQNVFG SFLKSEFSEE NIEFWLACED 130 140 150 160 YKKTESDLLP CKAEEIYKAF VHSDAAKQVS IKLIIISFSI KDPICRNNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Skeletal muscle and Thymus.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-209 (ISOFORM 1). Tissue: Skin.
[4]	"Isolation and characterization of a novel B cell activation gene." Hong J.X., Wilson G.L., Fox C.H., Kehrl J.H. J. Immunol. 150:3895-3904(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-209 (ISOFORM 1). Tissue: B-cell.
[5]	"A B cell specific immediate early human gene is located on chromosome band 1q31 and encodes an alpha helical basic phosphoprotein." Newton J.S., Deed R.W., Mitchell E.L.D., Murphy J.J., Norton J.D. Biochim. Biophys. Acta 1216:314-316(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-209 (ISOFORM 1). Tissue: B-cell.
[6]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 14-209 (ISOFORM 1).
[7]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 14-209 (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK303138 mRNA. Translation: BAG64242.1. Different initiation.
AK315607 mRNA. Translation: BAG37976.1. Different initiation.
AL136987 Genomic DNA. Translation: CAC19805.1.
BC015510 mRNA. Translation: AAH15510.1. Different initiation.
S59049 mRNA. Translation: AAB26289.1. Different initiation.
X73427 mRNA. Translation: CAA51826.1. Different initiation.
AF493925 mRNA. Translation: AAM12639.1.
BT006668 mRNA. Translation: AAP35314.1.

IPI

IPI00873849.
IPI00908845.

PIR

S43436.

RefSeq

NP_002913.3. NM_002922.3.

UniGene

Hs.75256.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BV1	X-ray	2.00	A/B	63-205	[»]
2GTP	X-ray	2.55	C/D	63-205	[»]

ProteinModelPortal

Q08116.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59091N.

IntAct

Q08116. 4 interactions.

MINT

MINT-1367963.

STRING

9606.ENSP00000356429.

PTM databases

PhosphoSite

Q08116.

Polymorphism databases

DMDM

229470360.

Proteomic databases

PaxDb

Q08116.

PRIDE

Q08116.

Protocols and materials databases

DNASU

5996.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000367459; ENSP00000356429; ENSG00000090104.
ENST00000469578; ENSP00000464323; ENSG00000090104.

GeneID

5996.

KEGG

hsa:5996.

UCSC

uc001gsi.1. human.
uc010pou.1. human.

Organism-specific databases

CTD

5996.

GeneCards

GC01P192544.

H-InvDB

HIX0001431.

HGNC

HGNC:9991. RGS1.

MIM

600323. gene.

neXtProt

NX_Q08116.

PharmGKB

PA34361.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG312555.

HOGENOM

HOG000233512.

HOVERGEN

HBG013233.

InParanoid

Q08116.

KO

K16449.

OMA

KIYKAFV.

OrthoDB

EOG42822Z.

Gene expression databases

Bgee

Q08116.

CleanEx

HS_RGS1.

Genevestigator

Q08116.

GermOnline

ENSG00000090104. Homo sapiens.

Family and domain databases

Gene3D

1.10.196.10. 2 hits.

InterPro

IPR000342. Regulat_G_prot_signal.
IPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
[Graphical view]

Pfam

PF00615. RGS. 1 hit.
[Graphical view]

PRINTS

PR01301. RGSPROTEIN.

SMART

SM00315. RGS. 1 hit.
[Graphical view]

SUPFAM

SSF48097. Regulat_G_prot_signal_superfam. 1 hit.

PROSITE

PS50132. RGS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q08116.

GenomeRNAi

5996.

NextBio

23367.

SOURCE

Search...

Entry information

Entry name

RGS1_HUMAN

Accession

Primary (citable) accession number: Q08116
Secondary accession number(s): B2RDM9 , B4DZY0, Q07918, Q9H1W2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 127 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.