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Q08108

- PLB3_YEAST

UniProt

Q08108 - PLB3_YEAST

Protein

Lysophospholipase 3

Gene

PLB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. Substrate preference is phosphatidylserine > phosphatidylinositol. Does not cleave phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid and phosphatidylinositol-bisphosphate.1 Publication

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

    GO - Molecular functioni

    1. lysophospholipase activity Source: SGD

    GO - Biological processi

    1. phosphatidylinositol metabolic process Source: SGD
    2. phosphatidylserine catabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciMetaCyc:YOL011W-MONOMER.
    YEAST:YOL011W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysophospholipase 3 (EC:3.1.1.5)
    Alternative name(s):
    Phospholipase B 3
    Gene namesi
    Name:PLB3
    Ordered Locus Names:YOL011W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL011w.
    SGDiS000005371. PLB3.

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: SGD
    3. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 659633Lysophospholipase 3PRO_0000024648Add
    BLAST
    Propeptidei660 – 68627Removed in mature formSequence AnalysisPRO_0000372444Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi221 – 2211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi283 – 2831N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi313 – 3131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi495 – 4951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi519 – 5191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi547 – 5471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi571 – 5711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi588 – 5881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi614 – 6141N-linked (GlcNAc...)Sequence Analysis
    Lipidationi659 – 6591GPI-anchor amidated asparagineSequence Analysis

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    MaxQBiQ08108.
    PaxDbiQ08108.

    Expressioni

    Gene expression databases

    GenevestigatoriQ08108.

    Interactioni

    Protein-protein interaction databases

    BioGridi34393. 48 interactions.
    DIPiDIP-4220N.
    MINTiMINT-535513.
    STRINGi4932.YOL011W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08108.
    SMRiQ08108. Positions 88-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 592554PLA2cPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi627 – 64317Poly-SerAdd
    BLAST

    Sequence similaritiesi

    Belongs to the lysophospholipase family.Curated
    Contains 1 PLA2c domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG271081.
    GeneTreeiENSGT00510000051940.
    HOGENOMiHOG000189547.
    KOiK13333.
    OMAiVYLNNFT.
    OrthoDBiEOG7N37NC.

    Family and domain databases

    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view]
    PfamiPF01735. PLA2_B. 1 hit.
    [Graphical view]
    SMARTiSM00022. PLAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52151. SSF52151. 1 hit.
    PROSITEiPS51210. PLA2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q08108-1 [UniParc]FASTAAdd to Basket

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    MIRPLCSKII ISYIFAISQF LLAANAWSPT DSYVPGTVSC PDDINLVREA    50
    TSISQNESAW LEKRNKVTSV ALKDFLTRAT ANFSDSSEVL SKLFNDGNSE 100
    NLPKIAVAVS GGGYRSMLTG AGVLAAMDNR TEGAYEHGLG GLLQSTTYLS 150
    GASGGNWLVG TLALNNWTSV QDILNNMQND DSIWDLSDSI VTPGGINIFK 200
    TAKRWDHISN AVESKQNADY NTSLADIWGR ALAYNFFPSL NRGGIGLTWS 250
    SIRDFPVFQN AEMPFPISVA DGRYPGTKVI NLNATVFEFN PFEMGSWDPS 300
    LNSFANVKYL GTNVSNGVPL ERGKCTAGFD NAGFIMGTSS TLFNQFLLRI 350
    NSTHLPSFIT RLARHFLKDL SQDFNDIAVY SPNPFKDTKF LDSDYTTSIV 400
    DSDSLFLVDG GEDDENVPVL PLIQKERDVD IIFAVDNSAD MRLAWPDGSS 450
    LVHTYERQFV KQGQGMSFPY VPDTNTFVNL GLNKKPTFFG CDANNLTDLQ 500
    YIPPLVVYLP NAEYSFNSNQ SAFKLSYSES QRRSMIQNGF EIATRNNFTD 550
    DPEFMGCVGC AIIRRKQQAL NITLPPECET CFKNYCWNGT LDTTPLPDVE 600
    KDVHHSFINV NSFNSSIGQE ESLYAGSSAS QSSSSSSSSS SSSEIPSATA 650
    TLEKKAATNS GSHLSGISVK FSAMIMLTLL MFTGAV 686
    Length:686
    Mass (Da):75,077
    Last modified:November 1, 1997 - v1
    Checksum:i95A2DBF41BF3E20F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74753 Genomic DNA. Translation: CAA99010.1.
    BK006948 Genomic DNA. Translation: DAA10772.1.
    PIRiS66693.
    RefSeqiNP_014632.1. NM_001183265.1.

    Genome annotation databases

    EnsemblFungiiYOL011W; YOL011W; YOL011W.
    GeneIDi854151.
    KEGGisce:YOL011W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74753 Genomic DNA. Translation: CAA99010.1 .
    BK006948 Genomic DNA. Translation: DAA10772.1 .
    PIRi S66693.
    RefSeqi NP_014632.1. NM_001183265.1.

    3D structure databases

    ProteinModelPortali Q08108.
    SMRi Q08108. Positions 88-439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34393. 48 interactions.
    DIPi DIP-4220N.
    MINTi MINT-535513.
    STRINGi 4932.YOL011W.

    Proteomic databases

    MaxQBi Q08108.
    PaxDbi Q08108.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL011W ; YOL011W ; YOL011W .
    GeneIDi 854151.
    KEGGi sce:YOL011W.

    Organism-specific databases

    CYGDi YOL011w.
    SGDi S000005371. PLB3.

    Phylogenomic databases

    eggNOGi NOG271081.
    GeneTreei ENSGT00510000051940.
    HOGENOMi HOG000189547.
    KOi K13333.
    OMAi VYLNNFT.
    OrthoDBi EOG7N37NC.

    Enzyme and pathway databases

    BioCyci MetaCyc:YOL011W-MONOMER.
    YEAST:YOL011W-MONOMER.

    Miscellaneous databases

    NextBioi 975905.

    Gene expression databases

    Genevestigatori Q08108.

    Family and domain databases

    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view ]
    Pfami PF01735. PLA2_B. 1 hit.
    [Graphical view ]
    SMARTi SM00022. PLAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52151. SSF52151. 1 hit.
    PROSITEi PS51210. PLA2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Characterization and function in vivo of two novel phospholipases B/lysophospholipases from Saccharomyces cerevisiae."
      Merkel O., Fido M., Mayr J.A., Prueger H., Raab F., Zandonella G., Kohlwein S.D., Paltauf F.
      J. Biol. Chem. 274:28121-28127(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPLB3_YEAST
    AccessioniPrimary (citable) accession number: Q08108
    Secondary accession number(s): D6W256
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3