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Q08108 (PLB3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysophospholipase 3

EC=3.1.1.5
Alternative name(s):
Phospholipase B 3
Gene names
Name:PLB3
Ordered Locus Names:YOL011W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length686 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. Substrate preference is phosphatidylserine > phosphatidylinositol. Does not cleave phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid and phosphatidylinositol-bisphosphate. Ref.3

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Potential Ref.3.

Sequence similarities

Belongs to the lysophospholipase family.

Contains 1 PLA2c domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 659633Lysophospholipase 3
PRO_0000024648
Propeptide660 – 68627Removed in mature form Potential
PRO_0000372444

Regions

Domain39 – 592554PLA2c
Compositional bias627 – 64317Poly-Ser

Amino acid modifications

Lipidation6591GPI-anchor amidated asparagine Potential
Glycosylation561N-linked (GlcNAc...) Potential
Glycosylation821N-linked (GlcNAc...) Potential
Glycosylation1291N-linked (GlcNAc...) Potential
Glycosylation1661N-linked (GlcNAc...) Potential
Glycosylation2211N-linked (GlcNAc...) Potential
Glycosylation2831N-linked (GlcNAc...) Potential
Glycosylation3131N-linked (GlcNAc...) Potential
Glycosylation3511N-linked (GlcNAc...) Potential
Glycosylation4951N-linked (GlcNAc...) Potential
Glycosylation5191N-linked (GlcNAc...) Potential
Glycosylation5471N-linked (GlcNAc...) Potential
Glycosylation5711N-linked (GlcNAc...) Potential
Glycosylation5881N-linked (GlcNAc...) Potential
Glycosylation6141N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q08108 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 95A2DBF41BF3E20F

FASTA68675,077
        10         20         30         40         50         60 
MIRPLCSKII ISYIFAISQF LLAANAWSPT DSYVPGTVSC PDDINLVREA TSISQNESAW 

        70         80         90        100        110        120 
LEKRNKVTSV ALKDFLTRAT ANFSDSSEVL SKLFNDGNSE NLPKIAVAVS GGGYRSMLTG 

       130        140        150        160        170        180 
AGVLAAMDNR TEGAYEHGLG GLLQSTTYLS GASGGNWLVG TLALNNWTSV QDILNNMQND 

       190        200        210        220        230        240 
DSIWDLSDSI VTPGGINIFK TAKRWDHISN AVESKQNADY NTSLADIWGR ALAYNFFPSL 

       250        260        270        280        290        300 
NRGGIGLTWS SIRDFPVFQN AEMPFPISVA DGRYPGTKVI NLNATVFEFN PFEMGSWDPS 

       310        320        330        340        350        360 
LNSFANVKYL GTNVSNGVPL ERGKCTAGFD NAGFIMGTSS TLFNQFLLRI NSTHLPSFIT 

       370        380        390        400        410        420 
RLARHFLKDL SQDFNDIAVY SPNPFKDTKF LDSDYTTSIV DSDSLFLVDG GEDDENVPVL 

       430        440        450        460        470        480 
PLIQKERDVD IIFAVDNSAD MRLAWPDGSS LVHTYERQFV KQGQGMSFPY VPDTNTFVNL 

       490        500        510        520        530        540 
GLNKKPTFFG CDANNLTDLQ YIPPLVVYLP NAEYSFNSNQ SAFKLSYSES QRRSMIQNGF 

       550        560        570        580        590        600 
EIATRNNFTD DPEFMGCVGC AIIRRKQQAL NITLPPECET CFKNYCWNGT LDTTPLPDVE 

       610        620        630        640        650        660 
KDVHHSFINV NSFNSSIGQE ESLYAGSSAS QSSSSSSSSS SSSEIPSATA TLEKKAATNS 

       670        680 
GSHLSGISVK FSAMIMLTLL MFTGAV 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Characterization and function in vivo of two novel phospholipases B/lysophospholipases from Saccharomyces cerevisiae."
Merkel O., Fido M., Mayr J.A., Prueger H., Raab F., Zandonella G., Kohlwein S.D., Paltauf F.
J. Biol. Chem. 274:28121-28127(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z74753 Genomic DNA. Translation: CAA99010.1.
BK006948 Genomic DNA. Translation: DAA10772.1.
PIRS66693.
RefSeqNP_014632.1. NM_001183265.1.

3D structure databases

ProteinModelPortalQ08108.
SMRQ08108. Positions 88-439.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34393. 48 interactions.
DIPDIP-4220N.
MINTMINT-535513.
STRING4932.YOL011W.

Proteomic databases

PaxDbQ08108.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL011W; YOL011W; YOL011W.
GeneID854151.
KEGGsce:YOL011W.

Organism-specific databases

CYGDYOL011w.
SGDS000005371. PLB3.

Phylogenomic databases

eggNOGNOG271081.
GeneTreeENSGT00510000051940.
HOGENOMHOG000189547.
KOK13333.
OMAVYLNNFT.
OrthoDBEOG7N37NC.

Enzyme and pathway databases

BioCycYEAST:YOL011W-MONOMER.

Gene expression databases

GenevestigatorQ08108.

Family and domain databases

InterProIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMSSF52151. SSF52151. 1 hit.
PROSITEPS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975905.

Entry information

Entry namePLB3_YEAST
AccessionPrimary (citable) accession number: Q08108
Secondary accession number(s): D6W256
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families