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Protein

Lysophospholipase 3

Gene

PLB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Sequentially removes both fatty acyl groups from diacylglycerophospholipids and therefore has both phospholipase A and lysophospholipase activities. Substrate preference is phosphatidylserine > phosphatidylinositol. Does not cleave phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid and phosphatidylinositol-bisphosphate.1 Publication

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

GO - Molecular functioni

  • lysophospholipase activity Source: SGD

GO - Biological processi

  • phosphatidylinositol metabolic process Source: SGD
  • phosphatidylserine catabolic process Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YOL011W-MONOMER.
YEAST:YOL011W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysophospholipase 3 (EC:3.1.1.5)
Alternative name(s):
Phospholipase B 3
Gene namesi
Name:PLB3
Ordered Locus Names:YOL011W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL011W.
SGDiS000005371. PLB3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000002464827 – 659Lysophospholipase 3Add BLAST633
PropeptideiPRO_0000372444660 – 686Removed in mature formSequence analysisAdd BLAST27

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi56N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi82N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi129N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi221N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi283N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi313N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi351N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi495N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi519N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi547N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi571N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi588N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi614N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi659GPI-anchor amidated asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ08108.
PRIDEiQ08108.

Interactioni

Protein-protein interaction databases

BioGridi34393. 85 interactors.
DIPiDIP-4220N.
IntActiQ08108. 1 interactor.
MINTiMINT-535513.
STRINGi4932.YOL011W.

Structurei

3D structure databases

ProteinModelPortaliQ08108.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 592PLA2cPROSITE-ProRule annotationAdd BLAST554

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi627 – 643Poly-SerAdd BLAST17

Sequence similaritiesi

Belongs to the lysophospholipase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00510000051940.
HOGENOMiHOG000189547.
InParanoidiQ08108.
KOiK13333.
OMAiTANIHNW.
OrthoDBiEOG092C2IZ9.

Family and domain databases

InterProiView protein in InterPro
IPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
PfamiView protein in Pfam
PF01735. PLA2_B. 1 hit.
SMARTiView protein in SMART
SM00022. PLAc. 1 hit.
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiView protein in PROSITE
PS51210. PLA2C. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08108-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRPLCSKII ISYIFAISQF LLAANAWSPT DSYVPGTVSC PDDINLVREA
60 70 80 90 100
TSISQNESAW LEKRNKVTSV ALKDFLTRAT ANFSDSSEVL SKLFNDGNSE
110 120 130 140 150
NLPKIAVAVS GGGYRSMLTG AGVLAAMDNR TEGAYEHGLG GLLQSTTYLS
160 170 180 190 200
GASGGNWLVG TLALNNWTSV QDILNNMQND DSIWDLSDSI VTPGGINIFK
210 220 230 240 250
TAKRWDHISN AVESKQNADY NTSLADIWGR ALAYNFFPSL NRGGIGLTWS
260 270 280 290 300
SIRDFPVFQN AEMPFPISVA DGRYPGTKVI NLNATVFEFN PFEMGSWDPS
310 320 330 340 350
LNSFANVKYL GTNVSNGVPL ERGKCTAGFD NAGFIMGTSS TLFNQFLLRI
360 370 380 390 400
NSTHLPSFIT RLARHFLKDL SQDFNDIAVY SPNPFKDTKF LDSDYTTSIV
410 420 430 440 450
DSDSLFLVDG GEDDENVPVL PLIQKERDVD IIFAVDNSAD MRLAWPDGSS
460 470 480 490 500
LVHTYERQFV KQGQGMSFPY VPDTNTFVNL GLNKKPTFFG CDANNLTDLQ
510 520 530 540 550
YIPPLVVYLP NAEYSFNSNQ SAFKLSYSES QRRSMIQNGF EIATRNNFTD
560 570 580 590 600
DPEFMGCVGC AIIRRKQQAL NITLPPECET CFKNYCWNGT LDTTPLPDVE
610 620 630 640 650
KDVHHSFINV NSFNSSIGQE ESLYAGSSAS QSSSSSSSSS SSSEIPSATA
660 670 680
TLEKKAATNS GSHLSGISVK FSAMIMLTLL MFTGAV
Length:686
Mass (Da):75,077
Last modified:November 1, 1997 - v1
Checksum:i95A2DBF41BF3E20F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74753 Genomic DNA. Translation: CAA99010.1.
BK006948 Genomic DNA. Translation: DAA10772.1.
PIRiS66693.
RefSeqiNP_014632.1. NM_001183265.1.

Genome annotation databases

EnsemblFungiiYOL011W; YOL011W; YOL011W.
GeneIDi854151.
KEGGisce:YOL011W.

Similar proteinsi

Entry informationi

Entry nameiPLB3_YEAST
AccessioniPrimary (citable) accession number: Q08108
Secondary accession number(s): D6W256
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 22, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names