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Q080J4 (ACKA_SHEFN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetate kinase

EC=2.7.2.1
Alternative name(s):
Acetokinase
Gene names
Name:ackA
Ordered Locus Names:Sfri_2476
OrganismShewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]
Taxonomic identifier318167 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction By similarity. HAMAP-Rule MF_00020

Catalytic activity

ATP + acetate = ADP + acetyl phosphate. HAMAP-Rule MF_00020

Cofactor

Mg2+. Can also accept Mn2+ By similarity. HAMAP-Rule MF_00020

Pathway

Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. HAMAP-Rule MF_00020

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00020

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00020.

Sequence similarities

Belongs to the acetokinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processacetyl-CoA biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

organic acid metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

acetate kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400Acetate kinase HAMAP-Rule MF_00020
PRO_1000002254

Regions

Nucleotide binding208 – 2125ATP By similarity
Nucleotide binding283 – 2853ATP By similarity
Nucleotide binding331 – 3355ATP By similarity

Sites

Active site1481Proton donor/acceptor By similarity
Metal binding101Magnesium By similarity
Metal binding3851Magnesium By similarity
Binding site171ATP By similarity
Binding site911Substrate By similarity
Site1801Transition state stabilizer By similarity
Site2411Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q080J4 [UniParc].

Last modified October 31, 2006. Version 1.
Checksum: EE08E536A64B94E1

FASTA40043,437
        10         20         30         40         50         60 
MSDKLVLVLN CGSSSLKFAI IDAQSGDDKV SGLAECFGLE DSRIKWKFDG GKNEAKLGAF 

        70         80         90        100        110        120 
TAHREAVEFI VNNILAEHPE LAAQIKAVGH RIVHGGEKFT RSVIVTPEVI QGIEDCASLA 

       130        140        150        160        170        180 
PLHNPAHLIG IRAALASFPA LPQVAVFDTA FHQTMPEKAY IYALPYKLYR EHSIRRYGMH 

       190        200        210        220        230        240 
GTSNIYICRE AAKVLGKETK DTNIICAHLG NGASVTAIKD GKSVDTSMGL TPLEGLVMGT 

       250        260        270        280        290        300 
RCGDLDPSII FHLVKQLGYT LDEVNNLLNK QSGLLGISEL TNDCRGIEEG YQDGHKGATL 

       310        320        330        340        350        360 
ALDIFCYRLA KYIASYTVPL GRLDAIIFTG GIGENSDLIR EKVLNLLAIF NFKVDDNLNK 

       370        380        390        400 
AARFGQQGII TQVGSPIAMV IPTNEEWVIA EDAIKLITAK 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000447 Genomic DNA. Translation: ABI72321.1.
RefSeqYP_751159.1. NC_008345.1.

3D structure databases

ProteinModelPortalQ080J4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING318167.Sfri_2476.

Proteomic databases

PRIDEQ080J4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI72321; ABI72321; Sfri_2476.
GeneID4279387.
KEGGsfr:Sfri_2476.
PATRIC23499001. VBISheFri14343_2571.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0282.
HOGENOMHOG000288398.
KOK00925.
OMAKYVTQRA.
OrthoDBEOG69975F.

Enzyme and pathway databases

BioCycSFRI318167:GIXS-2559-MONOMER.
UniPathwayUPA00340; UER00458.

Family and domain databases

HAMAPMF_00020. Acetate_kinase.
InterProIPR004372. Ac/Proprionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERPTHR21060. PTHR21060. 1 hit.
PfamPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSPR00471. ACETATEKNASE.
TIGRFAMsTIGR00016. ackA. 1 hit.
PROSITEPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACKA_SHEFN
AccessionPrimary (citable) accession number: Q080J4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 31, 2006
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways