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Q080C3 (DDL_SHEFN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Sfri_2550
OrganismShewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]
Taxonomic identifier318167 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000074787

Regions

Domain124 – 330207ATP-grasp
Nucleotide binding154 – 20956ATP By similarity

Sites

Metal binding2841Magnesium or manganese 1 By similarity
Metal binding2971Magnesium or manganese 1 By similarity
Metal binding2971Magnesium or manganese 2 By similarity
Metal binding2991Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q080C3 [UniParc].

Last modified October 31, 2006. Version 1.
Checksum: 053ED18BF968F6B0

FASTA33637,635
        10         20         30         40         50         60 
MPKINLLLLC GGGGAEHDIS LMSARFFESS LAKSDKFSVL KLVLDKHGHY HTEDGKLCDL 

        70         80         90        100        110        120 
TNRREIRFED NSTPAWSVDY VIPCIHGFPG ETGDIQSYFN LIQLPYFGCE SEASSNCFNK 

       130        140        150        160        170        180 
ITAKMWFSAL GVPNTPYIFL HQLDDDAIKQ TETAFDNWGS VFVKAASQGS SVGCYKVDVK 

       190        200        210        220        230        240 
ANIANVLKDA FSYAPYVVVE QTIHARELEV AVYQYQGEVV ATLPGEIICD SNTFYSFDEK 

       250        260        270        280        290        300 
YATNSKARTD VVADNLSPEI SQLIRDYAVK AFKGMKLRHL SRIDFFLTAD NQILLNEINT 

       310        320        330 
FPGLTPISMF PKMLQNHGHN FTEYLIDVIG QQVDLT 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000447 Genomic DNA. Translation: ABI72392.1.
RefSeqYP_751230.1. NC_008345.1.

3D structure databases

ProteinModelPortalQ080C3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING318167.Sfri_2550.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI72392; ABI72392; Sfri_2550.
GeneID4279458.
KEGGsfr:Sfri_2550.
PATRIC23499157. VBISheFri14343_2648.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycSFRI318167:GIXS-2634-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_SHEFN
AccessionPrimary (citable) accession number: Q080C3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 31, 2006
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways