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Q08096

- RCL1_YEAST

UniProt

Q08096 - RCL1_YEAST

Protein

RNA 3'-terminal phosphate cyclase-like protein

Gene

RCL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Does not have cyclase activity. Plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. Essential for viability.

    GO - Molecular functioni

    1. endoribonuclease activity Source: SGD
    2. enzyme activator activity Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    2. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    3. endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    4. positive regulation of catalytic activity Source: GOC
    5. rRNA processing Source: MGI

    Keywords - Biological processi

    Ribosome biogenesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33427-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA 3'-terminal phosphate cyclase-like protein
    Gene namesi
    Name:RCL1
    Synonyms:RTC1
    Ordered Locus Names:YOL010W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL010w.
    SGDiS000005370. RCL1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleolus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 367366RNA 3'-terminal phosphate cyclase-like proteinPRO_0000156444Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ08096.
    PaxDbiQ08096.
    PeptideAtlasiQ08096.

    Expressioni

    Gene expression databases

    GenevestigatoriQ08096.

    Interactioni

    Subunit structurei

    Associates with U3 snoRNP but is not its structural component. Associates with RCL1.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BMS1Q089654EBI-14892,EBI-3683

    Protein-protein interaction databases

    BioGridi34394. 37 interactions.
    DIPiDIP-4539N.
    IntActiQ08096. 17 interactions.
    MINTiMINT-566090.
    STRINGi4932.YOL010W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08096.
    SMRiQ08096. Positions 7-361.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0430.
    GeneTreeiENSGT00530000063404.
    HOGENOMiHOG000157670.
    KOiK11108.
    OMAiFMKHPLR.
    OrthoDBiEOG7PZS7V.

    Family and domain databases

    Gene3Di3.65.10.20. 2 hits.
    InterProiIPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR016443. RNA3'_term_phos_cyc_type_2.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view]
    PANTHERiPTHR11096. PTHR11096. 1 hit.
    PfamiPF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
    SUPFAMiSSF55205. SSF55205. 1 hit.
    TIGRFAMsiTIGR03400. 18S_RNA_Rcl1p. 1 hit.
    PROSITEiPS01287. RTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q08096-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV    50
    SFLRLIESVT NGSVIEISYT GTTVIYRPGI IVGGASTHIC PSSKPVGYFV 100
    EPMLYLAPFS KKKFSILFKG ITASHNDAGI EAIKWGLMPV MEKFGVRECA 150
    LHTLKRGSPP LGGGEVHLVV DSLIAQPITM HEIDRPIISS ITGVAYSTRV 200
    SPSLVNRMID GAKKVLKNLQ CEVNITADVW RGENSGKSPG WGITLVAQSK 250
    QKGWSYFAED IGDAGSIPEE LGEKVACQLL EEISKSAAVG RNQLPLAIVY 300
    MVIGKEDIGR LRINKEQIDE RFIILLRDIK KIFNTEVFLK PVDEADNEDM 350
    IATIKGIGFT NTSKKIA 367
    Length:367
    Mass (Da):40,171
    Last modified:November 1, 1996 - v1
    Checksum:iC5DDA7F9B0C1E296
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74752 Genomic DNA. Translation: CAA99009.1.
    BK006948 Genomic DNA. Translation: DAA10773.1.
    PIRiS66692.
    RefSeqiNP_014633.1. NM_001183264.1.

    Genome annotation databases

    EnsemblFungiiYOL010W; YOL010W; YOL010W.
    GeneIDi854152.
    KEGGisce:YOL010W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z74752 Genomic DNA. Translation: CAA99009.1 .
    BK006948 Genomic DNA. Translation: DAA10773.1 .
    PIRi S66692.
    RefSeqi NP_014633.1. NM_001183264.1.

    3D structure databases

    ProteinModelPortali Q08096.
    SMRi Q08096. Positions 7-361.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34394. 37 interactions.
    DIPi DIP-4539N.
    IntActi Q08096. 17 interactions.
    MINTi MINT-566090.
    STRINGi 4932.YOL010W.

    Proteomic databases

    MaxQBi Q08096.
    PaxDbi Q08096.
    PeptideAtlasi Q08096.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL010W ; YOL010W ; YOL010W .
    GeneIDi 854152.
    KEGGi sce:YOL010W.

    Organism-specific databases

    CYGDi YOL010w.
    SGDi S000005370. RCL1.

    Phylogenomic databases

    eggNOGi COG0430.
    GeneTreei ENSGT00530000063404.
    HOGENOMi HOG000157670.
    KOi K11108.
    OMAi FMKHPLR.
    OrthoDBi EOG7PZS7V.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33427-MONOMER.

    Miscellaneous databases

    NextBioi 975908.
    PROi Q08096.

    Gene expression databases

    Genevestigatori Q08096.

    Family and domain databases

    Gene3Di 3.65.10.20. 2 hits.
    InterProi IPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR016443. RNA3'_term_phos_cyc_type_2.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view ]
    PANTHERi PTHR11096. PTHR11096. 1 hit.
    Pfami PF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
    SUPFAMi SSF55205. SSF55205. 1 hit.
    TIGRFAMsi TIGR03400. 18S_RNA_Rcl1p. 1 hit.
    PROSITEi PS01287. RTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Rcl1p, the yeast protein similar to the RNA 3'-phosphate cyclase, associates with U3 snoRNP and is required for 18S rRNA biogenesis."
      Billy E., Wegierski T., Nasr F., Filipowicz W.
      EMBO J. 19:2115-2126(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. "Bms1p, a G-domain-containing protein, associates with Rcl1p and is required for 18S rRNA biogenesis in yeast."
      Wegierski T., Billy E., Nasr F., Filipowicz W.
      RNA 7:1254-1267(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BMS1.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRCL1_YEAST
    AccessioniPrimary (citable) accession number: Q08096
    Secondary accession number(s): D6W257
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 10000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3