Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA 3'-terminal phosphate cyclase-like protein

Gene

RCL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Does not have cyclase activity. Plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. Essential for viability.

GO - Molecular functioni

  • endoribonuclease activity Source: SGD
  • enzyme activator activity Source: SGD

GO - Biological processi

  • endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
  • endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • positive regulation of catalytic activity Source: GOC
  • rRNA processing Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-33427-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase-like protein
Gene namesi
Name:RCL1
Synonyms:RTC1
Ordered Locus Names:YOL010W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XV

Organism-specific databases

CYGDiYOL010w.
EuPathDBiFungiDB:YOL010W.
SGDiS000005370. RCL1.

Subcellular locationi

GO - Cellular componenti

  • 90S preribosome Source: GO_Central
  • nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 367366RNA 3'-terminal phosphate cyclase-like proteinPRO_0000156444Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ08096.
PaxDbiQ08096.
PeptideAtlasiQ08096.

Expressioni

Gene expression databases

GenevestigatoriQ08096.

Interactioni

Subunit structurei

Associates with U3 snoRNP but is not its structural component. Associates with RCL1.

Binary interactionsi

WithEntry#Exp.IntActNotes
BMS1Q089654EBI-14892,EBI-3683

Protein-protein interaction databases

BioGridi34394. 38 interactions.
DIPiDIP-4539N.
IntActiQ08096. 17 interactions.
MINTiMINT-566090.
STRINGi4932.YOL010W.

Structurei

Secondary structure

1
367
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 146Combined sources
Helixi17 – 2711Combined sources
Beta strandi31 – 344Combined sources
Turni36 – 394Combined sources
Beta strandi40 – 423Combined sources
Helixi47 – 5913Combined sources
Beta strandi60 – 623Combined sources
Beta strandi64 – 674Combined sources
Beta strandi74 – 774Combined sources
Beta strandi84 – 896Combined sources
Helixi96 – 1049Combined sources
Helixi107 – 1093Combined sources
Beta strandi114 – 1207Combined sources
Beta strandi125 – 1273Combined sources
Helixi130 – 1356Combined sources
Helixi137 – 1437Combined sources
Beta strandi150 – 1556Combined sources
Turni159 – 1613Combined sources
Beta strandi164 – 1707Combined sources
Beta strandi190 – 20011Combined sources
Helixi203 – 21614Combined sources
Beta strandi219 – 23012Combined sources
Helixi233 – 2353Combined sources
Beta strandi240 – 24910Combined sources
Turni250 – 2534Combined sources
Beta strandi254 – 2618Combined sources
Helixi268 – 28417Combined sources
Beta strandi287 – 2893Combined sources
Turni291 – 2933Combined sources
Helixi294 – 3029Combined sources
Beta strandi306 – 3138Combined sources
Helixi315 – 3173Combined sources
Helixi320 – 33314Combined sources
Beta strandi338 – 3414Combined sources
Beta strandi350 – 3556Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CLQX-ray2.02A1-367[»]
ProteinModelPortaliQ08096.
SMRiQ08096. Positions 7-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0430.
GeneTreeiENSGT00530000063404.
HOGENOMiHOG000157670.
KOiK11108.
OMAiDMAVVYM.
OrthoDBiEOG7PZS7V.

Family and domain databases

Gene3Di3.65.10.20. 2 hits.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR016443. RNA3'_term_phos_cyc_type_2.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03400. 18S_RNA_Rcl1p. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV
60 70 80 90 100
SFLRLIESVT NGSVIEISYT GTTVIYRPGI IVGGASTHIC PSSKPVGYFV
110 120 130 140 150
EPMLYLAPFS KKKFSILFKG ITASHNDAGI EAIKWGLMPV MEKFGVRECA
160 170 180 190 200
LHTLKRGSPP LGGGEVHLVV DSLIAQPITM HEIDRPIISS ITGVAYSTRV
210 220 230 240 250
SPSLVNRMID GAKKVLKNLQ CEVNITADVW RGENSGKSPG WGITLVAQSK
260 270 280 290 300
QKGWSYFAED IGDAGSIPEE LGEKVACQLL EEISKSAAVG RNQLPLAIVY
310 320 330 340 350
MVIGKEDIGR LRINKEQIDE RFIILLRDIK KIFNTEVFLK PVDEADNEDM
360
IATIKGIGFT NTSKKIA
Length:367
Mass (Da):40,171
Last modified:November 1, 1996 - v1
Checksum:iC5DDA7F9B0C1E296
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74752 Genomic DNA. Translation: CAA99009.1.
BK006948 Genomic DNA. Translation: DAA10773.1.
PIRiS66692.
RefSeqiNP_014633.1. NM_001183264.1.

Genome annotation databases

EnsemblFungiiYOL010W; YOL010W; YOL010W.
GeneIDi854152.
KEGGisce:YOL010W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74752 Genomic DNA. Translation: CAA99009.1.
BK006948 Genomic DNA. Translation: DAA10773.1.
PIRiS66692.
RefSeqiNP_014633.1. NM_001183264.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4CLQX-ray2.02A1-367[»]
ProteinModelPortaliQ08096.
SMRiQ08096. Positions 7-361.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34394. 38 interactions.
DIPiDIP-4539N.
IntActiQ08096. 17 interactions.
MINTiMINT-566090.
STRINGi4932.YOL010W.

Proteomic databases

MaxQBiQ08096.
PaxDbiQ08096.
PeptideAtlasiQ08096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL010W; YOL010W; YOL010W.
GeneIDi854152.
KEGGisce:YOL010W.

Organism-specific databases

CYGDiYOL010w.
EuPathDBiFungiDB:YOL010W.
SGDiS000005370. RCL1.

Phylogenomic databases

eggNOGiCOG0430.
GeneTreeiENSGT00530000063404.
HOGENOMiHOG000157670.
KOiK11108.
OMAiDMAVVYM.
OrthoDBiEOG7PZS7V.

Enzyme and pathway databases

BioCyciYEAST:G3O-33427-MONOMER.

Miscellaneous databases

NextBioi975908.
PROiQ08096.

Gene expression databases

GenevestigatoriQ08096.

Family and domain databases

Gene3Di3.65.10.20. 2 hits.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR016443. RNA3'_term_phos_cyc_type_2.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03400. 18S_RNA_Rcl1p. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Rcl1p, the yeast protein similar to the RNA 3'-phosphate cyclase, associates with U3 snoRNP and is required for 18S rRNA biogenesis."
    Billy E., Wegierski T., Nasr F., Filipowicz W.
    EMBO J. 19:2115-2126(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Bms1p, a G-domain-containing protein, associates with Rcl1p and is required for 18S rRNA biogenesis in yeast."
    Wegierski T., Billy E., Nasr F., Filipowicz W.
    RNA 7:1254-1267(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BMS1.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRCL1_YEAST
AccessioniPrimary (citable) accession number: Q08096
Secondary accession number(s): D6W257
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 27, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.