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Q08096

- RCL1_YEAST

UniProt

Q08096 - RCL1_YEAST

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Protein

RNA 3'-terminal phosphate cyclase-like protein

Gene

RCL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Does not have cyclase activity. Plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. Essential for viability.

GO - Molecular functioni

  1. endoribonuclease activity Source: SGD
  2. enzyme activator activity Source: SGD

GO - Biological processi

  1. endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  2. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  3. endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  4. positive regulation of catalytic activity Source: GOC
  5. rRNA processing Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-33427-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase-like protein
Gene namesi
Name:RCL1
Synonyms:RTC1
Ordered Locus Names:YOL010W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL010w.
SGDiS000005370. RCL1.

Subcellular locationi

GO - Cellular componenti

  1. nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 367366RNA 3'-terminal phosphate cyclase-like proteinPRO_0000156444Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ08096.
PaxDbiQ08096.
PeptideAtlasiQ08096.

Expressioni

Gene expression databases

GenevestigatoriQ08096.

Interactioni

Subunit structurei

Associates with U3 snoRNP but is not its structural component. Associates with RCL1.

Binary interactionsi

WithEntry#Exp.IntActNotes
BMS1Q089654EBI-14892,EBI-3683

Protein-protein interaction databases

BioGridi34394. 38 interactions.
DIPiDIP-4539N.
IntActiQ08096. 17 interactions.
MINTiMINT-566090.
STRINGi4932.YOL010W.

Structurei

3D structure databases

ProteinModelPortaliQ08096.
SMRiQ08096. Positions 7-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0430.
GeneTreeiENSGT00530000063404.
HOGENOMiHOG000157670.
KOiK11108.
OMAiFMKHPLR.
OrthoDBiEOG7PZS7V.

Family and domain databases

Gene3Di3.65.10.20. 2 hits.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR016443. RNA3'_term_phos_cyc_type_2.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03400. 18S_RNA_Rcl1p. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08096 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV
60 70 80 90 100
SFLRLIESVT NGSVIEISYT GTTVIYRPGI IVGGASTHIC PSSKPVGYFV
110 120 130 140 150
EPMLYLAPFS KKKFSILFKG ITASHNDAGI EAIKWGLMPV MEKFGVRECA
160 170 180 190 200
LHTLKRGSPP LGGGEVHLVV DSLIAQPITM HEIDRPIISS ITGVAYSTRV
210 220 230 240 250
SPSLVNRMID GAKKVLKNLQ CEVNITADVW RGENSGKSPG WGITLVAQSK
260 270 280 290 300
QKGWSYFAED IGDAGSIPEE LGEKVACQLL EEISKSAAVG RNQLPLAIVY
310 320 330 340 350
MVIGKEDIGR LRINKEQIDE RFIILLRDIK KIFNTEVFLK PVDEADNEDM
360
IATIKGIGFT NTSKKIA
Length:367
Mass (Da):40,171
Last modified:November 1, 1996 - v1
Checksum:iC5DDA7F9B0C1E296
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74752 Genomic DNA. Translation: CAA99009.1.
BK006948 Genomic DNA. Translation: DAA10773.1.
PIRiS66692.
RefSeqiNP_014633.1. NM_001183264.1.

Genome annotation databases

EnsemblFungiiYOL010W; YOL010W; YOL010W.
GeneIDi854152.
KEGGisce:YOL010W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z74752 Genomic DNA. Translation: CAA99009.1 .
BK006948 Genomic DNA. Translation: DAA10773.1 .
PIRi S66692.
RefSeqi NP_014633.1. NM_001183264.1.

3D structure databases

ProteinModelPortali Q08096.
SMRi Q08096. Positions 7-361.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34394. 38 interactions.
DIPi DIP-4539N.
IntActi Q08096. 17 interactions.
MINTi MINT-566090.
STRINGi 4932.YOL010W.

Proteomic databases

MaxQBi Q08096.
PaxDbi Q08096.
PeptideAtlasi Q08096.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL010W ; YOL010W ; YOL010W .
GeneIDi 854152.
KEGGi sce:YOL010W.

Organism-specific databases

CYGDi YOL010w.
SGDi S000005370. RCL1.

Phylogenomic databases

eggNOGi COG0430.
GeneTreei ENSGT00530000063404.
HOGENOMi HOG000157670.
KOi K11108.
OMAi FMKHPLR.
OrthoDBi EOG7PZS7V.

Enzyme and pathway databases

BioCyci YEAST:G3O-33427-MONOMER.

Miscellaneous databases

NextBioi 975908.
PROi Q08096.

Gene expression databases

Genevestigatori Q08096.

Family and domain databases

Gene3Di 3.65.10.20. 2 hits.
InterProi IPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR016443. RNA3'_term_phos_cyc_type_2.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view ]
PANTHERi PTHR11096. PTHR11096. 1 hit.
Pfami PF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view ]
PIRSFi PIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMi SSF55205. SSF55205. 1 hit.
TIGRFAMsi TIGR03400. 18S_RNA_Rcl1p. 1 hit.
PROSITEi PS01287. RTC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Rcl1p, the yeast protein similar to the RNA 3'-phosphate cyclase, associates with U3 snoRNP and is required for 18S rRNA biogenesis."
    Billy E., Wegierski T., Nasr F., Filipowicz W.
    EMBO J. 19:2115-2126(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Bms1p, a G-domain-containing protein, associates with Rcl1p and is required for 18S rRNA biogenesis in yeast."
    Wegierski T., Billy E., Nasr F., Filipowicz W.
    RNA 7:1254-1267(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BMS1.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRCL1_YEAST
AccessioniPrimary (citable) accession number: Q08096
Secondary accession number(s): D6W257
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3