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Protein

RNA 3'-terminal phosphate cyclase-like protein

Gene

RCL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Does not have cyclase activity. Plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. Essential for viability.

GO - Molecular functioni

  • endoribonuclease activity Source: SGD
  • enzyme activator activity Source: SGD
  • RNA binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-33427-MONOMER.
ReactomeiR-SCE-6790901. rRNA modification in the nucleus and cytosol.
R-SCE-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase-like protein
Gene namesi
Name:RCL1
Synonyms:RTC1
Ordered Locus Names:YOL010W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL010W.
SGDiS000005370. RCL1.

Subcellular locationi

GO - Cellular componenti

  • 90S preribosome Source: GO_Central
  • nucleolus Source: SGD
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001564442 – 367RNA 3'-terminal phosphate cyclase-like proteinAdd BLAST366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ08096.
PRIDEiQ08096.

Interactioni

Subunit structurei

Associates with U3 snoRNP but is not its structural component. Associates with RCL1.

Binary interactionsi

WithEntry#Exp.IntActNotes
BMS1Q089654EBI-14892,EBI-3683

Protein-protein interaction databases

BioGridi34394. 40 interactors.
DIPiDIP-4539N.
IntActiQ08096. 19 interactors.
MINTiMINT-566090.

Structurei

Secondary structure

1367
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 14Combined sources6
Helixi17 – 27Combined sources11
Beta strandi31 – 34Combined sources4
Turni36 – 39Combined sources4
Beta strandi40 – 42Combined sources3
Helixi47 – 59Combined sources13
Beta strandi60 – 62Combined sources3
Beta strandi64 – 67Combined sources4
Beta strandi74 – 77Combined sources4
Beta strandi84 – 89Combined sources6
Helixi96 – 104Combined sources9
Helixi107 – 109Combined sources3
Beta strandi114 – 120Combined sources7
Beta strandi125 – 127Combined sources3
Helixi130 – 135Combined sources6
Helixi137 – 143Combined sources7
Beta strandi150 – 155Combined sources6
Turni159 – 161Combined sources3
Beta strandi164 – 170Combined sources7
Beta strandi190 – 200Combined sources11
Helixi203 – 216Combined sources14
Beta strandi219 – 230Combined sources12
Helixi233 – 235Combined sources3
Beta strandi240 – 249Combined sources10
Turni250 – 253Combined sources4
Beta strandi254 – 261Combined sources8
Helixi268 – 284Combined sources17
Beta strandi287 – 289Combined sources3
Turni291 – 293Combined sources3
Helixi294 – 302Combined sources9
Beta strandi306 – 313Combined sources8
Helixi315 – 317Combined sources3
Helixi320 – 333Combined sources14
Beta strandi338 – 341Combined sources4
Beta strandi350 – 355Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CLQX-ray2.02A1-367[»]
5JPQelectron microscopy7.30Z1-367[»]
ProteinModelPortaliQ08096.
SMRiQ08096.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000063404.
HOGENOMiHOG000157670.
KOiK11108.
OMAiQGSQNFR.
OrthoDBiEOG092C3Z6E.

Family and domain databases

CDDicd00875. RNA_Cyclase_Class_I. 1 hit.
Gene3Di3.65.10.20. 2 hits.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR016443. RNA3'_term_phos_cyc_type_2.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03400. 18S_RNA_Rcl1p. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV
60 70 80 90 100
SFLRLIESVT NGSVIEISYT GTTVIYRPGI IVGGASTHIC PSSKPVGYFV
110 120 130 140 150
EPMLYLAPFS KKKFSILFKG ITASHNDAGI EAIKWGLMPV MEKFGVRECA
160 170 180 190 200
LHTLKRGSPP LGGGEVHLVV DSLIAQPITM HEIDRPIISS ITGVAYSTRV
210 220 230 240 250
SPSLVNRMID GAKKVLKNLQ CEVNITADVW RGENSGKSPG WGITLVAQSK
260 270 280 290 300
QKGWSYFAED IGDAGSIPEE LGEKVACQLL EEISKSAAVG RNQLPLAIVY
310 320 330 340 350
MVIGKEDIGR LRINKEQIDE RFIILLRDIK KIFNTEVFLK PVDEADNEDM
360
IATIKGIGFT NTSKKIA
Length:367
Mass (Da):40,171
Last modified:November 1, 1996 - v1
Checksum:iC5DDA7F9B0C1E296
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74752 Genomic DNA. Translation: CAA99009.1.
BK006948 Genomic DNA. Translation: DAA10773.1.
PIRiS66692.
RefSeqiNP_014633.1. NM_001183264.1.

Genome annotation databases

EnsemblFungiiYOL010W; YOL010W; YOL010W.
GeneIDi854152.
KEGGisce:YOL010W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74752 Genomic DNA. Translation: CAA99009.1.
BK006948 Genomic DNA. Translation: DAA10773.1.
PIRiS66692.
RefSeqiNP_014633.1. NM_001183264.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CLQX-ray2.02A1-367[»]
5JPQelectron microscopy7.30Z1-367[»]
ProteinModelPortaliQ08096.
SMRiQ08096.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34394. 40 interactors.
DIPiDIP-4539N.
IntActiQ08096. 19 interactors.
MINTiMINT-566090.

Proteomic databases

MaxQBiQ08096.
PRIDEiQ08096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL010W; YOL010W; YOL010W.
GeneIDi854152.
KEGGisce:YOL010W.

Organism-specific databases

EuPathDBiFungiDB:YOL010W.
SGDiS000005370. RCL1.

Phylogenomic databases

GeneTreeiENSGT00530000063404.
HOGENOMiHOG000157670.
KOiK11108.
OMAiQGSQNFR.
OrthoDBiEOG092C3Z6E.

Enzyme and pathway databases

BioCyciYEAST:G3O-33427-MONOMER.
ReactomeiR-SCE-6790901. rRNA modification in the nucleus and cytosol.
R-SCE-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Miscellaneous databases

PROiQ08096.

Family and domain databases

CDDicd00875. RNA_Cyclase_Class_I. 1 hit.
Gene3Di3.65.10.20. 2 hits.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR016443. RNA3'_term_phos_cyc_type_2.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03400. 18S_RNA_Rcl1p. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRCL1_YEAST
AccessioniPrimary (citable) accession number: Q08096
Secondary accession number(s): D6W257
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.