Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q08096 (RCL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA 3'-terminal phosphate cyclase-like protein
Gene names
Name:RCL1
Synonyms:RTC1
Ordered Locus Names:YOL010W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Does not have cyclase activity. Plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. Essential for viability.

Subunit structure

Associates with U3 snoRNP but is not its structural component. Associates with RCL1.

Subcellular location

Nucleusnucleolus.

Miscellaneous

Present with 10000 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BMS1Q089654EBI-14892,EBI-3683

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 367366RNA 3'-terminal phosphate cyclase-like protein
PRO_0000156444

Amino acid modifications

Modified residue21N-acetylserine Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q08096 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C5DDA7F9B0C1E296

FASTA36740,171
        10         20         30         40         50         60 
MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV SFLRLIESVT 

        70         80         90        100        110        120 
NGSVIEISYT GTTVIYRPGI IVGGASTHIC PSSKPVGYFV EPMLYLAPFS KKKFSILFKG 

       130        140        150        160        170        180 
ITASHNDAGI EAIKWGLMPV MEKFGVRECA LHTLKRGSPP LGGGEVHLVV DSLIAQPITM 

       190        200        210        220        230        240 
HEIDRPIISS ITGVAYSTRV SPSLVNRMID GAKKVLKNLQ CEVNITADVW RGENSGKSPG 

       250        260        270        280        290        300 
WGITLVAQSK QKGWSYFAED IGDAGSIPEE LGEKVACQLL EEISKSAAVG RNQLPLAIVY 

       310        320        330        340        350        360 
MVIGKEDIGR LRINKEQIDE RFIILLRDIK KIFNTEVFLK PVDEADNEDM IATIKGIGFT 


NTSKKIA 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Rcl1p, the yeast protein similar to the RNA 3'-phosphate cyclase, associates with U3 snoRNP and is required for 18S rRNA biogenesis."
Billy E., Wegierski T., Nasr F., Filipowicz W.
EMBO J. 19:2115-2126(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Bms1p, a G-domain-containing protein, associates with Rcl1p and is required for 18S rRNA biogenesis in yeast."
Wegierski T., Billy E., Nasr F., Filipowicz W.
RNA 7:1254-1267(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BMS1.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z74752 Genomic DNA. Translation: CAA99009.1.
BK006948 Genomic DNA. Translation: DAA10773.1.
PIRS66692.
RefSeqNP_014633.1. NM_001183264.1.

3D structure databases

ProteinModelPortalQ08096.
SMRQ08096. Positions 7-361.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34394. 37 interactions.
DIPDIP-4539N.
IntActQ08096. 17 interactions.
MINTMINT-566090.
STRING4932.YOL010W.

Proteomic databases

MaxQBQ08096.
PaxDbQ08096.
PeptideAtlasQ08096.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL010W; YOL010W; YOL010W.
GeneID854152.
KEGGsce:YOL010W.

Organism-specific databases

CYGDYOL010w.
SGDS000005370. RCL1.

Phylogenomic databases

eggNOGCOG0430.
GeneTreeENSGT00530000063404.
HOGENOMHOG000157670.
KOK11108.
OMAFMKHPLR.
OrthoDBEOG7PZS7V.

Enzyme and pathway databases

BioCycYEAST:G3O-33427-MONOMER.

Gene expression databases

GenevestigatorQ08096.

Family and domain databases

Gene3D3.65.10.20. 2 hits.
InterProIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR016443. RNA3'_term_phos_cyc_type_2.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERPTHR11096. PTHR11096. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMSSF55205. SSF55205. 1 hit.
TIGRFAMsTIGR03400. 18S_RNA_Rcl1p. 1 hit.
PROSITEPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975908.
PROQ08096.

Entry information

Entry nameRCL1_YEAST
AccessionPrimary (citable) accession number: Q08096
Secondary accession number(s): D6W257
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families