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Protein

Calponin-1

Gene

Cnn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calponin-1
Alternative name(s):
Basic calponin
Calponin H1, smooth muscle
Gene namesi
Name:Cnn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:104979. Cnn1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297Calponin-1PRO_0000204768Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei170 – 1701Phosphothreonine; by ROCK2By similarity
Modified residuei175 – 1751Phosphoserine; by ROCK2By similarity
Modified residuei180 – 1801Phosphothreonine; by ROCK2By similarity
Modified residuei184 – 1841Phosphothreonine; by ROCK2By similarity
Modified residuei259 – 2591Phosphothreonine; by ROCK2By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ08091.
MaxQBiQ08091.
PaxDbiQ08091.
PRIDEiQ08091.

PTM databases

iPTMnetiQ08091.
PhosphoSiteiQ08091.

Expressioni

Tissue specificityi

Smooth muscle, and tissues containing significant amounts of smooth muscle.

Gene expression databases

BgeeiQ08091.
CleanExiMM_CNN1.
ExpressionAtlasiQ08091. baseline and differential.
GenevisibleiQ08091. MM.

Interactioni

Subunit structurei

Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001384.

Structurei

3D structure databases

ProteinModelPortaliQ08091.
SMRiQ08091. Positions 13-142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 131104CHPROSITE-ProRule annotationAdd
BLAST
Repeati164 – 18926Calponin-like 1Add
BLAST
Repeati204 – 22926Calponin-like 2Add
BLAST
Repeati243 – 26826Calponin-like 3Add
BLAST

Sequence similaritiesi

Belongs to the calponin family.Curated
Contains 3 calponin-like repeats.PROSITE-ProRule annotation
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2046. Eukaryota.
COG5199. LUCA.
GeneTreeiENSGT00550000074447.
HOGENOMiHOG000232113.
HOVERGENiHBG005186.
InParanoidiQ08091.
OMAiRQIFEPT.
OrthoDBiEOG71P2B3.
PhylomeDBiQ08091.
TreeFamiTF313921.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001997. Calponin/LIMCH1.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamiPF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
[Graphical view]
PRINTSiPR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q08091-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF
60 70 80 90 100
MDGLKDGIIL CEFINKLQPG SVKKVNESTQ NWHQLENIGN FIKAITKYGV
110 120 130 140 150
KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER
160 170 180 190 200
RFEPEKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD
210 220 230 240 250
QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN
260 270 280 290
KGASQRGMTV YGLPRQVYDP KYCLNPEYPE LSEPTHNHHP HNYYNSA
Length:297
Mass (Da):33,356
Last modified:October 1, 1994 - v1
Checksum:i0D1F1F31899700ED
GO
Isoform Beta (identifier: Q08091-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-256: Missing.

Show »
Length:257
Mass (Da):29,154
Checksum:iFCD47E474974BECB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351R → E in AAB01453 (Ref. 2) Curated
Sequence conflicti35 – 351R → E in AAB01452 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei217 – 25640Missing in isoform Beta. CuratedVSP_000755Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19542 mRNA. Translation: CAA79602.1.
L49022 Genomic DNA. Translation: AAB01453.1.
L49022 Genomic DNA. Translation: AAB01452.1.
U28932 Genomic DNA. Translation: AAC52448.1.
U40351
, U38930, U40348, U40349, U40350 Genomic DNA. Translation: AAC52353.1.
CCDSiCCDS22921.1. [Q08091-1]
PIRiS36145. S31486.
RefSeqiNP_034052.3. NM_009922.4. [Q08091-1]
UniGeneiMm.4356.

Genome annotation databases

EnsembliENSMUST00000001384; ENSMUSP00000001384; ENSMUSG00000001349. [Q08091-1]
GeneIDi12797.
KEGGimmu:12797.
UCSCiuc009onv.2. mouse. [Q08091-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19542 mRNA. Translation: CAA79602.1.
L49022 Genomic DNA. Translation: AAB01453.1.
L49022 Genomic DNA. Translation: AAB01452.1.
U28932 Genomic DNA. Translation: AAC52448.1.
U40351
, U38930, U40348, U40349, U40350 Genomic DNA. Translation: AAC52353.1.
CCDSiCCDS22921.1. [Q08091-1]
PIRiS36145. S31486.
RefSeqiNP_034052.3. NM_009922.4. [Q08091-1]
UniGeneiMm.4356.

3D structure databases

ProteinModelPortaliQ08091.
SMRiQ08091. Positions 13-142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001384.

PTM databases

iPTMnetiQ08091.
PhosphoSiteiQ08091.

Proteomic databases

EPDiQ08091.
MaxQBiQ08091.
PaxDbiQ08091.
PRIDEiQ08091.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001384; ENSMUSP00000001384; ENSMUSG00000001349. [Q08091-1]
GeneIDi12797.
KEGGimmu:12797.
UCSCiuc009onv.2. mouse. [Q08091-1]

Organism-specific databases

CTDi1264.
MGIiMGI:104979. Cnn1.

Phylogenomic databases

eggNOGiKOG2046. Eukaryota.
COG5199. LUCA.
GeneTreeiENSGT00550000074447.
HOGENOMiHOG000232113.
HOVERGENiHBG005186.
InParanoidiQ08091.
OMAiRQIFEPT.
OrthoDBiEOG71P2B3.
PhylomeDBiQ08091.
TreeFamiTF313921.

Miscellaneous databases

PROiQ08091.
SOURCEiSearch...

Gene expression databases

BgeeiQ08091.
CleanExiMM_CNN1.
ExpressionAtlasiQ08091. baseline and differential.
GenevisibleiQ08091. MM.

Family and domain databases

Gene3Di1.10.418.10. 1 hit.
InterProiIPR001997. Calponin/LIMCH1.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
[Graphical view]
PfamiPF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
[Graphical view]
PRINTSiPR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTiSM00033. CH. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiPS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian calponin. Identification and expression of genetic variants."
    Strasser P., Gimona M., Moessler H., Herzog M., Small J.V.
    FEBS Lett. 330:13-18(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Smooth muscle.
  2. Gao J.M., Hwang J.M., Resek M.E., Jin J.P.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  3. Miano J.M., Olson E.N.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
    Tissue: Liver.
  4. "Developmental pattern of expression and genomic organization of the calponin-h1 gene. A contractile smooth muscle cell marker."
    Samaha F.F., Ip H.S., Morrisey E.E., Seltzer J., Tang Z., Solway J., Parmacek M.S.
    J. Biol. Chem. 271:395-403(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Lung, Spleen and Testis.

Entry informationi

Entry nameiCNN1_MOUSE
AccessioniPrimary (citable) accession number: Q08091
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 8, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.