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Protein

Calponin-1

Gene

Cnn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calponin-1
Alternative name(s):
Basic calponin
Calponin H1, smooth muscle
Gene namesi
Name:Cnn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:104979. Cnn1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002047681 – 297Calponin-1Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170Phosphothreonine; by ROCK2By similarity1
Modified residuei175Phosphoserine; by ROCK2By similarity1
Modified residuei180Phosphothreonine; by ROCK2By similarity1
Modified residuei184Phosphothreonine; by ROCK2By similarity1
Modified residuei259Phosphothreonine; by ROCK2By similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08091.
PaxDbiQ08091.
PeptideAtlasiQ08091.
PRIDEiQ08091.

PTM databases

iPTMnetiQ08091.
PhosphoSitePlusiQ08091.

Expressioni

Tissue specificityi

Smooth muscle, and tissues containing significant amounts of smooth muscle.

Gene expression databases

BgeeiENSMUSG00000001349.
CleanExiMM_CNN1.
ExpressionAtlasiQ08091. baseline and differential.
GenevisibleiQ08091. MM.

Interactioni

Subunit structurei

Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001384.

Structurei

3D structure databases

ProteinModelPortaliQ08091.
SMRiQ08091.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 131Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST104
Repeati164 – 189Calponin-like 1Add BLAST26
Repeati204 – 229Calponin-like 2Add BLAST26
Repeati243 – 268Calponin-like 3Add BLAST26

Sequence similaritiesi

Belongs to the calponin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2046. Eukaryota.
COG5199. LUCA.
GeneTreeiENSGT00550000074447.
HOGENOMiHOG000232113.
HOVERGENiHBG005186.
InParanoidiQ08091.
OMAiAGMAKSK.
OrthoDBiEOG091G0LY2.
PhylomeDBiQ08091.
TreeFamiTF313921.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
InterProiView protein in InterPro
IPR036872. Calponin-like_dom_sf.
IPR001997. Calponin/LIMCH1.
IPR000557. Calponin_repeat.
IPR001715. CH-domain.
IPR003096. SM22_calponin.
PfamiView protein in Pfam
PF00402. Calponin. 3 hits.
PF00307. CH. 1 hit.
PRINTSiPR00889. CALPONIN.
PR00888. SM22CALPONIN.
SMARTiView protein in SMART
SM00033. CH. 1 hit.
SUPFAMiSSF47576. SSF47576. 1 hit.
PROSITEiView protein in PROSITE
PS01052. CALPONIN_1. 3 hits.
PS51122. CALPONIN_2. 3 hits.
PS50021. CH. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q08091-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSAHFNRGP AYGLSAEVKN KLAQKYDHQR EQELREWIEG VTGRRIGNNF
60 70 80 90 100
MDGLKDGIIL CEFINKLQPG SVKKVNESTQ NWHQLENIGN FIKAITKYGV
110 120 130 140 150
KPHDIFEAND LFENTNHTQV QSTLLALASM AKTKGNKVNV GVKYAEKQER
160 170 180 190 200
RFEPEKLREG RNIIGLQMGT NKFASQQGMT AYGTRRHLYD PKLGTDQPLD
210 220 230 240 250
QATISLQMGT NKGASQAGMT APGTKRQIFE PGLGMEHCDT LNVSLQMGSN
260 270 280 290
KGASQRGMTV YGLPRQVYDP KYCLNPEYPE LSEPTHNHHP HNYYNSA
Length:297
Mass (Da):33,356
Last modified:October 1, 1994 - v1
Checksum:i0D1F1F31899700ED
GO
Isoform Beta (identifier: Q08091-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-256: Missing.

Show »
Length:257
Mass (Da):29,154
Checksum:iFCD47E474974BECB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35R → E in AAB01453 (Ref. 2) Curated1
Sequence conflicti35R → E in AAB01452 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000755217 – 256Missing in isoform Beta. CuratedAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19542 mRNA. Translation: CAA79602.1.
L49022 Genomic DNA. Translation: AAB01453.1.
L49022 Genomic DNA. Translation: AAB01452.1.
U28932 Genomic DNA. Translation: AAC52448.1.
U40351
, U38930, U40348, U40349, U40350 Genomic DNA. Translation: AAC52353.1.
CCDSiCCDS22921.1. [Q08091-1]
PIRiS36145. S31486.
RefSeqiNP_034052.3. NM_009922.4. [Q08091-1]
UniGeneiMm.4356.

Genome annotation databases

EnsembliENSMUST00000001384; ENSMUSP00000001384; ENSMUSG00000001349. [Q08091-1]
GeneIDi12797.
KEGGimmu:12797.
UCSCiuc009onv.2. mouse. [Q08091-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCNN1_MOUSE
AccessioniPrimary (citable) accession number: Q08091
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 25, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families